Objective:To construct the recombinant plasmid pBI-Eg95 of E.granulosus,and to study its expression efficiency in the Agrobacterium tumefaciens(At) LBA4404 strain.Methods:Total RNA was extracted from hydatid cyst protoscoleces shattered by supersound.The Eg95 coding gene was amplified by RT-PCR,and then was cloned into the plant expression vector pBI121 to construct the recombinant plasmid pBI-Eg95.The recombinant plasmid was electroporated into At(LBA4404) strain.The recombinant At(rAt) was induced by IPTG for 0,1,3,5,7,9,11or13 hour,respectively.The expression product was identified by SDS-PAGE and Western blot assay.Results:The 471bp Eg95 coding gene was successfully amplified,and the recombinant pBI-Eg95 plasmid was successfully constructed,which was confirmed by restriction endonuclease digestion and PCR.The recombinant plasmid pBI-Eg95 was expressed successfully in the rAt,and the expression product with an approximately Mr of 16.5 kD could be detected by sera from mice infected by E.granulosus,and the expression efficiency was about 20 percent of the total bacterial protein.Conclusion:The recombinant plasmid could provide the basis for further research of the transgenic plant vaccine of E.granulosus.

Objective:To explore the relationships among narcissism,empathy and prosocial behaviors.Methods:Four hundred and eight participants finished Narcissism Personality Questionnaire,Hyper sensitivity Narcissism Scale,The Interpersonal Reactivity Index,and Questionnaire of Prosocial Behavior.Results:① Overt -narcissism was slightly correlated with covert -narcissism.② After controlling age and gender,overt narcissism could negatively predicate perspective taking,and personal distress,and positively predicate fantasy;and covert narcissism could negatively predicate perspective taking and empathic concern,and positively predicate fantasy and personal distress.③After controlling age,gender and empathy,overt narcissism could significantly predicate self-reported proscial behaviors.Conclusion:Overt narcissism and covert narcissism have differences;both overt and covert narcissists are lacking of empathy for others.

Objective:To investigate the antitumor effect of vitamin E succinate combined with tamoxifen on the graft model of human breast cancer in nude mice and to analyze the possible mechanism.Methods: MCF-7 human breast cancer cells were inoculated subcutaneously in 40 nude mice.Then the mice were equally divided into 4 groups: blank control group(non-treatment),TAM treatment group(5 mg/kg),VES treatment group(150 mg/kg) and the TAM+VES treatment group(TAM 5 mg/kg +VES 150 mg/kg).The size of the tumor was measured and both the cell cycle and cell surface Fas/FasL were determined by flow cytometry 4 weeks after treatment.Fas/FasL expression in tumor tissue was detected with immunohistochemistry and cell apoptosis was detected by TUNEL method.Results: The co-administration of VES and TAM had more potent inhibitory effect on the growth of solid tumor than VES or TAM administered alone(P

〔Abstract〕 The paper takes nearly 300 clinical doctors in 5 hospitals in Jining as the research objects .By questionnaire , it under-stands the dynamic factors , channels and obstacles for them to acquire professional information and their satisfaction , analyzes their infor-mation requirements and existing problems , and points out that libraries in medical colleges and universities should focus on the improve -ment of the authority , timeliness and comprehensive retrieval of professional information resources and provide local hospitals with services of the think tank .

A scientific and rational questionnaire for emotional work was designed according to the 5 factors and 30 items screened by item analysis, confidence analysis and efficiency test based on the references and twice questionnaire investigations, in order to provide reference for the emotional work management in academic libraries.

The paper analyzes the feasibility that libraries in medical universities and colleges perform information services for standardized training of Traditional Chinese Medicine (TCM) residents,and discusses the contents of information services,including the cultivation of professional skills,clinical thinking ability,scientific research ability and humanistic quality.It aims to expand characteristic services of libraries in medical universities and colleges,enhance their service ability and strengthen their position and influence.

Objective: To construct and identify the recombinant pCD-Eg95 plasmid of Echinococcus granulosus. Methods: Total RNA was extracted from hydatid cyst protoscoleces shattered by supersound. The Eg95 coding gene was amplified by RT-PCR, and then was cloned into the eukaryotic expression vector pCDNA3.1 to construct the recombinant pCD-Eg95 plasmid. The recombinant plasmid was electroporated into E. coliBL21(DE3) strain. Results: The 471 bp Eg95 coding gene was successfully amplified, and the recombinant pCD-Eg95 plasmid was successfully constructed, which was confirmed by restriction endonuclease digestion and PCR. Conclusion: The results could provide the basis for the further research of the nucleic acid vaccine of Echinococcus granulosus.

Aim To study the regulation mechanisms of deacetylase inhibitor SAHA in p21WAF1/CIP1 promoter acetylation in breast cancer MCF-7 cells.Methods We used quantitative real-time PCR, Western blot and DNA-ChIP to determine the effects on the regulation of cell cycle with SAHA treatment in MCF-7 cells.By DNA-ChIP, we assessed the acetylation level of p21WAF1/CIP1 promoter.Results SAHA significantly affected the expression of cell cycle-related factors, and induced the mRNA and protein expression of p21WAF1/CIP1.SAHA could adjust the acetylation level of p21WAF1/CIP1 promoter.Conclusion SAHA regulates the cell cycle progression by adjusting the acetylation level of p21WAF1/CIP1 promoter in MCF-7 cells.

Inspect the anchorage molars with band and the anchorage molars with buccal tube with dental plaque index and gum in- dex and then statistically process the objective data obtained.The results show that both wearing band and wearing buccal tube lead to dental plaque.The influence is less worse on the the anchorage molars with buccal tube.

By analyzing the Military Health System winning "Heliang & Heli" Fund Award between 1994 and 2009,we found that the Military Health System possessed a good scientific basis and high level,but with an unbalanced scientific research development,which suggests the Military Health System should further strengthen its independent innovation ability.