1.Prevention and treatment overview of deep vein thrombosis in perioperative period
International Journal of Traditional Chinese Medicine 2010;32(5):463-465
This article analyzed both the etiopathogenisis and high risk factors of DVT in patients of orthopaedics diseases. Furthermore, it explained both the prophylaxis and therapies of DVT from traditional Chinese medicine (TCM),western medicine, and combined traditional Chinese and western medicine point of view.
2.Blastic plasmacytoid dendritic cell neoplasm: report of a case.
Chinese Journal of Pathology 2011;40(10):710-711
Aged
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Antineoplastic Combined Chemotherapy Protocols
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therapeutic use
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CD4 Antigens
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metabolism
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CD56 Antigen
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metabolism
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Cyclophosphamide
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therapeutic use
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Dendritic Cells
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pathology
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Diagnosis, Differential
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Doxorubicin
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therapeutic use
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Female
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Humans
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Leukemia, Myeloid
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metabolism
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pathology
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Lymphoma, Extranodal NK-T-Cell
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metabolism
;
pathology
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Plasmacytoma
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drug therapy
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metabolism
;
pathology
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
;
metabolism
;
pathology
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Prednisone
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therapeutic use
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Skin Neoplasms
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drug therapy
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metabolism
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pathology
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Vincristine
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therapeutic use
3.Human infection due to Streptococcus suis.
Chinese Journal of Epidemiology 2005;26(9):645-648
4. The aberrant methylation of fragile histidine triad (FHIT) gene and its significance of the expression of FHIT protein in lung cancer
Tumor 2007;27(11):902-906
Objective: To detect the expression of fragile histidine triad (FHIT) protein and aberrant methylation of its gene in lung carcinoma tissue and discuss their significance in tumorigenesis of lung cancer. Methods: The expression of FHIT protein was determined by immunohistochemical SP staining and its aberrant methylation was detected by methylation-specific PCR (MSP) in lung cancer and adjacent lung tissues (n = 60). The sequencing of the amplified products of MSP was performed. The 60 patients were followed up. Results: The expression of FHIT protein in adjacent lung tissues was obviously higher than that in lung cancer tissues. There was significant difference between them (76.7% vs 50%, P < 0.05). The aberrant methylation of FHIT gene was significantly higher in lung cancer tissues than that in adjacent lung tissues (68.3% vs 35.0%, P <0.001). And the aberrant methylation of FHIT gene occurred frequently in FHIT-negative patients compared with FHIT-positive patients (83.3% vs 53.5%, P <0. 05). The expression of FHIT protein and the aberrant methylation of FHIT gene had no correlation with clinicopathological parameters (age, sex, smoking status, histological type, general classification, TNM staging, and lymph node metastasis) in the patients with lung cancer (P > 0.05). The survival time was longer in FHIT-positive patients than FHIT-negative patients (P <0.05). The FHIT protein was the risk factor associated with the disease-free survival time of patients (P <0.01). Conclusion: The aberrant methylation of FHIT gene occurrs frequently and the expression of FHIT protein is significantly down-regulated in lung cancer. FHIT may play an important role in the tumorigenesis and development of lung cancer. FHIT protein could be used as an important factor for predicting the prognosis of patients.
5. Expression of fragile histidine triad protein in lung cancer tissues and its correlation with multidrug resistance protein 1
Academic Journal of Second Military Medical University 2006;27(11):1218-1221
Objective: To investigate the expression of fragile histidine triad (FHIT) protein in lung carcinoma tissues and its relationship with multidrug resistance protein 1 (MDR1), and to discuss the clinical significance of the changes of FHIT expression. Methods: The expression of FHIT protein was detected in the lung cancerous tissues and the adjacent tissues (n = 60) using S-P staining method. The expression of MDR1 in lung carcinoma tissues was also detected. The expression of FHIT was analyzed in lung cancerous tissues with different clinicopathological parameters and its relationship with MDR1 was analyzed at the same time. Results: The expression of FHIT protein in the adjacent tissues was obviously higher than that in the lung cancerous tissues(76.7% vs 50%, P<0.01). No correlation was found between FHIT expression and the age, sex, smoking, general type, TNM staging, histological type, and lymph node metastasis in the patients with lung cancer. The expression of MDR1 protein in lung cancerous tissues positive for FHIT was obviously higher than those negative for FHIT(83.3% vs 60%, P<0.01). Conclusion: The expression of FHIT protein in lung cancer is evidently down regulated and is correlated with the expression of MDR1. Potential multiple drug resistance should be considered in FHIT gene therapy.
7.Discussion on the botanical origin of Isatidis radix and Isatidis folium based on DNA barcoding.
Acta Pharmaceutica Sinica 2013;48(12):1850-1855
This paper aimed to investigate the botanical origins of Isatidis Radix and Isatidis Folium, and clarify the confusion of its classification. The second internal transcribed spacer (ITS2) of ribosomal DNA, the chloroplast matK gene of 22 samples from some major production areas were amplified and sequenced. Sequence assembly and consensus sequence generation were performed using the CodonCode Aligner. Phylogenetic study was performed using MEGA 4.0 software in accordance with the Kimura 2-Parameter (K2P) model, and the phylogenetic tree was constructed using the neighbor-joining methods. The results showed that the length of ITS2 sequence of the botanical origins of Isatidis Radix and Isatidis Folium was 191 bp. The sequence showed that some samples had several SNP sites, and some samples had heterozygosis sites. In the NJ tree, based on ITS2 sequence, the studied samples were separated into two groups, and one of them was gathered with Isatis tinctoria L. The studied samples also were divided into two groups obviously based on the chloroplast matK gene. In conclusion, our results support that the botanical origins of Isatidis Radix and Isatidis Folium are Isatis indigotica Fortune, and Isatis indigotica and Isatis tinctoria are two distinct species. This study doesn't support the opinion about the combination of these two species in Flora of China.
Chloroplasts
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genetics
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DNA Barcoding, Taxonomic
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methods
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DNA, Plant
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genetics
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DNA, Ribosomal Spacer
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genetics
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Genes, Plant
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genetics
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Isatis
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classification
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genetics
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Phylogeny
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Plant Leaves
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genetics
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Plants, Medicinal
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classification
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genetics
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Species Specificity
8.Application of immunocytochemistry in the cytopathology.
Chinese Journal of Pathology 2005;34(10):672-673
10.Surgical progress on early stage non-small call lung cancer
Xiuyi ZHI ; Hui LI ; Donghong CHEN
Cancer Research and Clinic 2010;22(1):2-6
Surgical resection (usually lobectomy) is considered the treatment option for individuals with stage Ⅰ and Ⅱ non-small cell lung cancer. The surgical treatment of stage Ⅰ and Ⅱ non-small cell lung cancer (NSCLC) continues to evolve in the areas of intraoperative lymph node staging (specifically the issue of lymph node dissection vs sampling), the role of sublobular resections instead of lobectomy for treatment of smaller tumors (especially peripheral carcinoma ≤2 cm in diameter), and the use of video-assisted techniques to perform anatomic lobectomy. Video-assisted thoracic surgery (VATS) lobectomy provides a minimally invasive approach for the management of early-stage lung cancer. Questions about the safety of VATS lobectomy and its adequacy as a cancer operation compared with open thoraeotomy have hindered its universal acceptance among thoracic surgeons. Evidence suggests that VATS lobectomy can be safely performed and is an adequate cancer operation for early-stage NSCLC. Recently, robots have been introduced into surgical procedures in an attempt to facilitate surgical performance. However, adequately powered well-balanced studies comparing VATS with open thoracotomy for lobectomy are lacking in the literature.