In condition of direct vision on fundus of eye, microperimeter can quantitatively detect the retinal light sensitivity of macular area, and record real time tracking of the eye, automatically analyze the location and stability of fixation. Microperimeter matches fundus image with micro cyclogram point-to-point, thus it achieves the combination of visual function and structure. The characteristics of microperimeter have good relations with subjective vision, so it can be a new method for the accurate vision evaluation and has application potential to assess the visual function in legal medicine. In this ar-ticle, we summarize the principle, method and parameters of microperimeter. Also, the applications of microperimeter in vision assessment are focused in order to provide a reference for the assessment of visual function in the legal medicine.

AIM To study the effect l-menthol pretreated nasal cavity on insulin pharmacological bioavailability(PBA). METHODS To set up diabetic rats model,nasal cavities were pretreated with l-menthol for two hours,insulin was dripped, blood glucose concentrations of different time were determined,and the area over the curve (AOC) and pharmacological bioavailability(PBA) were calculated. RESULTS After rats nasal cavity was pretreated with different concentrations l-menthol, nasal cavity absorption of insulin markedly increased between 2 h and 3 h,and PBA of insulin nasal cavity absorption increased respectively 5.77,7.00 and 3.12 times than control group. CONCLUSION l-Menthol promotes the nasal cavity absorption of insulin.

AIM: To study the effects of l-menthol and its binary systems on percutaneous penetration and deposit effects of 5-fluorouracil (5-FU). METHODS: Percutaneous absorption experiment was performed on the apparatus of isolated skin, penetrated amounts of different times of 5-FU were determined, the cumulate penetrated amounts and permeation rates and increased permeation times were calculated, and the deposit effect of 5-FU was investigated. RESULTS: L-menthol of different concentrations could significantly enhance the percutaneous penetration of 5-FU, its increased permeation times were 1.25 and 1.45 and 1.37 times respectively,and the action of 5-FU with 2% l-menthol was the strongest. 5-FU with 2% and 4% l-menthol had a marked deposit effect. When some kinds of enhancers were used alone or combined with l-menthol, the percutaneous penetration of 5-FU had a significantly increase. CONCLUSION: L-menthol and its binary systems can markedly promote the percutaneous penetration and the deposit effect of 5-FU.

In condition of direct vision on hindus of eye, microperimeter can quantitatively detect the retinal light sensitivity of macular area, and record real time tracking of the eye, automatically analyze the location and stability of fixation. Microperimeter matches hindus image with micro cyclogram point-to-point, thus it achieves the combination of visual function and structure. The characteristics of microperimeter have good relations with subjective vision, so it can be a new method for the accurate vision evaluation and has application potential to assess the visual function in legal medicine. In this article, we summarize the principle, method and parameters of microperimeter. Also, the applications of microperimeter in vision assessment are focused in order to provide a reference for the assessment of visual function in the legal medicine.
Forensic Medicine/legislation & jurisprudence* ; Humans ; Retina ; Vision, Ocular ; Visual Acuity ; Visual Field Tests

Chronic Disease ; Humans ; Sinusitis ; diagnosis ; therapy

AIM: To investigate the nasal absorption of ligustrazine and promotion action of synthetic borneol. METHODS: In situ nasal recirculation method was used to study the dose effect relationship of nasal absorption of ligustrazine and the effect of synthetic borneol on nasal absorption of ligustrazine in rats. RESULTS: When the concentrations of ligustrazine were 0.25 , 0.75 ,and 1.25 g?L -1 in circulation liquid, respectively, the corresponding values of the absorption rate constant k were 0.0195 , 0.0227 , and 0.0241 min -1 . However, when the concentrations were 2.5 and 5.0 g?L -1 in circulation liquid, the absorption rate constant k lowered with the concentration being added. CONCLUSION: Ligustrazine can be absorbed through the nasal mucous and synthetic borneol can promote the absorption.

Objective:To investigate the effect of synthetic borneol on penetration mechanism of ligustrazine hydrochloride. Methods: Permeation tests in vitro through rabbit skin in two compartment diffusion cells were performed to study the effect of synthetic borneol on transdermal absorption of ligustrazine hydrochloride by changing concentrations of synthetic borneol and rabbit skin condition. Results: Ligustrazine hydrochloride permeation coefficient increased linearly with incerease of concentrations of synthetic borneol, while those through strippde stratum corneum skin were not varied. Synthetic borneol enhanced the deposit functions of whole skin and stripped stratum corneum skin. Conclusions: Synthetic borneol could enhance the percutaneous absorption of ligustrazine hydrochloride, mainly through the stratum corneum, and increase the deposit function.

Animals ; Bridged-Ring Compounds ; poisoning ; Disease Models, Animal ; Female ; Male ; Mice ; Sodium Oxybate ; therapeutic use ; Unithiol ; therapeutic use

BACKGROUND: Bone morphogenetic protein-2 has been previously proved to not only stimulate and different bone tissue-derived cells, but also induce differentiation from cell strain into osteoblasts; however, direct application of bone morphogenetic protein has poor effects on repairing bone defects. OBJECTIVE: To study new bone formation in a rabbit model of avascular necrosis of the femoral head (ANFH) following recombinant human bone morphogenetic protein-2 (rhBMP-2)/fibrin sealant (FS) implantation combining with core decompression. DESIGN, TIME AND SETTING: A randomized controlled animal experiment was performed at the Affiliated Hospital of Medical College of Chinese People’s Armed Police Force from January 2005 to December 2007. MATERIALS: Composite was made by rhBMP-2 and FS, and the final concentration of rhBMP-2 was 1 mg/L. METHODS: Animal models of ANFH were made by injecting hormone. The rabbits were randomly divided into three groups, including rhBMP-2/FS implantation group, rhBMP-2 implantation group, and core decompression alone group. MAIN OUTCOME MEASURES: Signal changes of femoral head and sclerotin were detected using MRI method; new bone formation was observed under optic microscopy; calcium content was measured using atomic absorrtion spectrophotometer. RESULTS: MRI indicated that new bone replaced primary bone defect channel at week 8 after rhBMP-2/FS implantation. A few of new bones were observed in the rhBMP-2 implantation group, and fiber tissue was still observed in the core decompression alone group. Morphology suggested that a great quantity of mature bone trabecula and plate-shaped bone replaced primary bone defect channel at week 8 after rhBMP-2/FS implantation. Bone defect was decreased in the rhBMP-2 implantation group, accompanying with a few of bone trabecula and blood capillary but a large quantity of fiber tissues. At week 8 after core decompression alone, bone defect was decreased, and a few of new bones were observed. Fiber tissue still existed in the center, and any bone tissue did not fill in it. Calcium content in the rhBMP-2/FS implantation group was greater than rhBMP-2 implantation group and core decompression alone group (P