ObjectiveTo explore the clinical effectiveness of intralesional glucocorticoid therapy on infantile hemangioma in proliferative stage,and variance of vascular endothelial growth factor(VEGF) expression in the hemangioma tissues.MethodsExperimental group:5 cases were injected with triamcinolone acetonide 3 days before operation,5 cases were done 6 days before operation,5 cases were done 9 days before operation and 5 cases were done 2 weeks before operation(the dose of injection was 1 mg/cm2 per time,but not exceeding 2 mg/kg).Control group:5 infantile hemangioma cases in involuting stage and 5 infantile hemangioma cases in proliferative stage did not received any therapy.The specimen of the hemangioma were collected to detect the expression of VEGF by two-step immunohistochemistry.Every optical slice was detected by 5 campus visualis of high power lens.ResultsObserving the specimen dyed by HE in the light microscope,there was no significant difference between injected group and non-injected group.But by means of immunohistochemistry,the expression of VEGF could been down regulation by the glucocorticoid(F=17.919 1P

Objective: To examine the regulatory effect of corticotrophin-releasing hormone (CRH) on glutamate-mediated current (IGLU) in cultured hippocampal neurons and to study the related mechanism. Methods: Immunofluorescence analysis was used to investigate whether the cultured hippocampal neurons express CRH receptors, and the whole-cell patch-clamp technique was used to examine the direct modulation of CRH on IGLU and the possible intracellular signal pathway. Results: Two minutes' exposure to CRH obviously depressed IGLU in the neurons in a dose-dependent manner. CRH receptor antagonist α-helical CRH or CRH receptor type 1 (CRHR1) antagonist antalarmin completely blocked CRH-induced depression of IGLU; whereas, CRH receptor type 2 (CRHR2) antagonist astressin-2B failed to block the effects of CRH. Application of the PKC inhibitor Gö6976 totally blocked the CRH-induced decrease of IGLU. Conclusion: CRH can inhibit IGLU in primary cultured hippocampal neurons, which is mediated by CRHR1 and may involve the PKC signal pathway.

Objective To explore diagnosis and prognosis of heart failure in children by measuring brain natriurettic peptide(BNP) and cardiac troponin I(CTnI).Methods Forty-one children subjects with heart failure were chosen,and then they were divided into three stages,early stage of heart failure,stage of heart failure and stage of recovery according to the ROSS cardiac function score method.In contrast with the former group,41 healthy children were chosen as control group.BNP and CTnI levels of blood plasma were analyzed by radioimmunoassay(RIA) and chemiluminescent immunoassay(CLIA) respectively,the relationship between the BNP level of blood plasma and prognosis of CHF group was analyzed.Results At early stage of heart failure,BNP and CTnI levels began to rise.They amount to highest peaks at stage of heart failure and went down at stage of recovery.But they were still higher than those of control group(P

Objective To analyze the clinical and molecular epidemiology in children with measles in Shanghai and identify the clinical characteristics and the prevalent genotype of measles virus.Methods The clinical features of measles-such as ages of onset,crowds and epidemiological data were retrospectively analyzed in 39 children with measles from Nov.2005 to Jun.2006 in Children′s Hospital Affiliated to Fudan University.Results of blood routine test,liver function test and chest X-ray were also included.Sputum examinations were carried out on the ones who had bronchitis or bronchopneumonia.Throat swab specimen within 3 days of the onset of rashes were collected and were sent to Center for Disease Control and Prevention (CDC) of Shanghai to isolate measles virus,then the genotypes of the isolated viruses were determined subsequently in CDC of China.Results Twenty-nine cases out of the 39 children were from foreign provinces (74.36%).Among of them,24 cases were younger than (or as young as) 9 months old.Among the 39 cases,35 children had never been inoculated with measles vaccine,and to speak of the ones who were younger than 8 months,the ratio was 57.14%.Five cases out of the 39 children had contacted with measles sufferers.Clinical manifestation were fever,rashes,Koplik spots and catarrh.Twenty-three cases were co-infected with respiratory system and only 8 cases were identified as infection with bacteria or other viruses.The genotype of all analyzed measles viruses belongs to H1a.Conclusions There are also some changes in age groups and endemic distribution,such as younger age and more immigrants except for the typical clinical manifestation of fever,rashes,Koplik spots and catarrh.The predominant genotype is still H1a as in most parts of China.There is no evidence to demonstrate any relationship between the raised morbidity of measles and the genovariation of measles virus.

Humans ; Infant, Low Birth Weight ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; diagnosis ; prevention & control ; therapy ; Intracranial Hemorrhages ; diagnosis ; prevention & control ; therapy ; Phenobarbital ; therapeutic use

Air Pollutants ; analysis ; Animals ; Child ; Esters ; toxicity ; Humans ; Liver ; drug effects ; Phthalic Acids ; analysis ; metabolism ; toxicity ; Reproduction ; drug effects ; Soil Pollutants ; analysis ; Thyroid Gland ; drug effects ; Water Pollutants, Chemical ; analysis

Adolescent ; Angiotensin-Converting Enzyme Inhibitors ; therapeutic use ; Blood Pressure ; drug effects ; Child ; Child, Preschool ; Female ; Fosinopril ; pharmacology ; therapeutic use ; Humans ; Male ; Nephritis ; drug therapy ; Proteinuria ; drug therapy ; Purpura, Schoenlein-Henoch ; complications

Child ; Child, Preschool ; Female ; Humans ; Infant ; Intercellular Adhesion Molecule-1 ; blood ; Leukocytes, Mononuclear ; metabolism ; Lymphocyte Function-Associated Antigen-1 ; blood ; Male ; Seizures, Febrile ; blood

Objective To observe the effect of Jianpi Yangrong Tablets (JYT) on mice with cyclophosphamide-induced low immunity. Methods Sixty NIH mice were equally randomized into 6 groups: low-, middle-and high-dose JYT groups (4、8 and 16gkg-1d-1 respectively), batyl alcohol group (0.055gkg-1d-1), model group and normal group. The mice received gastric gavage of the corresponding drug according to the experimental design. On the 5th day of medication, the mice received intraperitoneal injection of cyclophosphamide 200mg/kg to induce the models of low immunity. On the 9th day of medication, peripheral white blood cell (WBC) count, carbon clearance test, bone marrow nucleated cells count, spleen index and proliferative reaction of spleen lymphocyteswere observed.Results The number of WBC in middle-and high-dose JYT groups and batyl alcohol group was higher than that in the model group (P

NF-?B, a group of important transcription factors, are introduced and discussed here on several aspects: their component and molecular structure; their activity control by I?B and IKK, the mechanism of their activation; their important roles in transcriptional regulation for large numbers of genes; and their importance in immunity, inflammation, cell survival, proliferation and apoptosis. The analysis of the relation between NF-?B and disease occurrence, the analysis of the relation between NF-?B and disease therapy, and the application prospect of the new strategy regarding the novel drug design and correlative diseases therapy on the basis of NF-?B as the target, are also included.