Integrin is a main factor that mediates adhesive attraction between the cell and the extracellular matrix,and it participates in the formation of new vessel lumens and the construction of the rete vasculosum by regulating endothelial cell adhesion and migration.The extracellular matrix is the ligand of integrin.Collagen and laminin's deposition form new vessel lumens.The vascular endothelial growth factor promotes the proliferation of endothelial cells,accelerates angiogenesis and increases the permeability of blood vessels.This review focuses on the roles and correlation of integrin and VEGF in angiogenesis.

Humans ; Occupational Diseases ; diagnosis

Objective To explore the efficacy and safety of tocilizumab in combination with disease-modifying anti-rheumatoid drugs (DMARDs) for the treatment of rheumatoid arthritis (RA) patients with moderate to severe activity and inadequate response to DMARDs. Methods Thirty-two RA patients with inadequate response to DMARDs were treated with tocilizumab along with stable dose of DMARDs for 12 weeks, adverse reactions were recorded, clinical laboratory and physiological indices were recorded 4, 8, and 12 weeks after treatment. The routine blood, liver and kidney function tests, morning stiffness, rest pain, tender joint count, swollen joint count, overall evaluation of the patient and physician to disease and health assessment questionnaire, blood sedimentation, C-reactive protein (CRP), Disease activity score 28 (DAS28), simplified disease activity index (SDAI) score, clinical disease activity index (CDAI). Repetitive measure analysis of variance wase used for statistical analysis. Results The clinical laboratory indices and DAS28, SDAI, CDAI scores observed in all of the patients were significantly improved (P<0.05). After 8 and 12 weeks of treatment, disease activity was further improved with statistical significance (P<0.05). The levels of IL-6 were downregulated obvious compared with before [(26±14) pg/ml vs (76±39) pg/ml, t=-6.925, P<0.01], and no obvious adverse reactions were observed. Conclusion Tocilizumab can quickly improve the symptoms and the control disease activity of refractory active RA within a few weeks.

OBJECTIVE:To observe clinical efficacy and safety of sertraline hydrochloride combined with esomeprazole in the treatment of patients with gastroesophageal reflux disease complicating with depression. METHODS:80 patients with gastroesopha-geal reflux disease complicating with depression selected from our hospital were divided into control group and observation group according to random number table,with 40 cases in each group. Control group received Esomeprazole magnesium enteric-coated tablets orally 40 mg,qd;observation was additionally given Sertraline hydrochloride tablets 50 mg,qd,on the basis of control group. Both groups received treatment for 1 month. Clinical efficacies of 2 groups were observed as well as clinical symptom score,gastroesophageal reflux disease diagnostic questionnaire (GERD Q scale) score,SDS score. The occurrence of ADR was compared between 2 groups. RESULTS:The total effective rate of observation group 92.50%,which was significantly higher than 77.50% of control group,with statistical significance (P<0.05). Before treatment,there was no statistical significance in clinical symptom score,GERD Q scale score and SDS score(P>0.05). After treatment,above scores of 2 groups were improved signifi-cantly,and the observation group was significantly better than the control group,with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between 2 groups (P>0.05). CONCLUSIONS:Sertraline hydrochloride com-bined with esomeprazole shows significantly therapeutic efficacy in the treatment of patients with gastroesophageal reflux disease complicating with depression,and can effectively alleviate the clinical symptoms and improve depression with good safety.

Objective To study the value of Oxytocin challenge test (OCT) in high-risk pregnancy with abnormal umbilical artery resistance and the clinical significance of performing the umbilical velocimetry during OCT. Methods One hundred and seventy patients with abnormal umbilical artery resistance but maintained forward diastolic flow and 178 patients with normal umbilical blood flow received OCT, umbilical velocimetry was conducted during OCT. If the umbilical artery pulsatility index (PI) increased more than 0.5 during contraction and OCT was positive, cesarean section was performed in the same day, otherwise, vaginal delivery was tried. Results The rate of positive OCT was 45.3% and 10.1% respectively and the rate of positive OCT with umbilical artery PI increasing more than 0.5 was 37.4% and 6.7% respectively in abnormal umbilical artery flow resistance group and normal umbilical artery flow group ( P

Objective To explore the relationship between KISS-1 gene and metastasis of bladder carcinoma,and to study the effect of the stable expression of KISS-1 gene on the invasion of bladder carcinoma cell line T24.Methods Fluorescent quantitative PCR was used to detect the expression of KISS-1 mRNA in primary bladder carcinoma without metastasis and primary bladder carcinoma with metastasis.Recombinant vector pIRES2-AKS-1 was constructed and transfected into T24 cells.Single clone of stably transfected cells was screened,and the changes in the invasive ability of T24 cells was detected after transfection.Results The expression level of KISS-1 mRNA in primary bladder carcinoma with metastasis was significantly lower than that in primary bladder carcinoma without metastasis(P＜0.05).The expression of KISS-1 protein in the single clone of stably transfected cells increased significantly,and the invasive ability significantly decreased(P＜0.05).Conclusion KISS-1 gene is correlated with the metastasis of bladder carcinoma,and the up-regulated expression of KISS-1 gene can inhibite the invasiveness of T24 cell line.

Objective To observe the effect of exercise on insulin resistance induced by a high fat diet and to explore the underlying mechanism.Methods Thirty healthy male Wistar rats were randomly divided into a normal control group (NC group) and a high-fat group (HF group),fed with normal chow and a high fat diet respectively.Eighteen weeks later,insulin resistance had appeared in the HF group.The HF group was then randomly subdivided into a high-fat diet control group (HC group,fed a high fat diet) and an exercise group (HE group,fed a high fat diet and subjected to swimming training for 6 weeks).After 24 weeks,an insulin sensitivity index was calculated.Serum free fatty acid (FFA) and skeletal muscle triglyceride (TG) were detected.Phosphorylation of adenosine monophosphate kinase-α (AMPKα) in skeletal muscle was detected by Western blotting.Results At twenty-four weeks the insulin sensitivity of the HC group had decreased significantly compared to the NC group.Serum FFA level and skeletal muscle TG content had both increased.Average phosphorylation of AMPKα in the HC group decreased to 48.7% of the NC group average.Compared to the HC group,insulin sensitivity and phosporylation of AMPK-α were elevated significantly in the HE group.Serum FFA level and skeletal muscle TG content were both lower.However,compared to the NC group,both serum FFA level and skeletal muscle TG content had increased in the HE group,while insulin sensitivity and phosphorylation of AMPKα had significantly decreased.Conclusions Exercise can significantly improve insulin resistance induced by a high-fat diet through decreasing serum FFA level and ectopic li-pid accumulation in skeletal muscle.

Objective:The infusion pumps are high risk equipment in clinic, the clinical risk of it is much higher the general medical equipment and diagnostic. Methods:FLUKE lagu1 was used to calibrate the Smiths infusion pumps in different conditions. Results: As a result, the quality control of it is particularly important. We introduce a method to calibrate model 1200 infusion pump from the angle of quality control. Conclusion: Infusion pump quality control testing should accord with clinical practice. Infusion pumps calibrated to provide security for the clinical infusion therapy and reduce the medical risk.

Medical devices are widely used in the treatment of diseases,health care and rehabilitation process as modern science and technology products,and become an important means in the field of modern medicine. How to ensure that medical devices are used safe and effective is the common problem in production,management,use and monitoring.

Objective To study the clinical effects of shortening the drainage time for patients with dural tears after posterior spinal sur -gery.Methods A total of 120 patients with dural tears after posterior spinal surgery were randomly divided into study group and control group,60 patients in each group .Patients in control group had wound drainage tubes removed after 5 days and patients in study group had wound drainage tubes removed after 3 days.The disappearance time of leakage ,incision healing time and complication rate were compared between two groups.Results The disappearance time of leakage in study group was (13.7 ±3.8)days which was significantly less than (20.0 ±5.1)days in control group(P<0.05).The incision healing time in study group was (22.7 ±4.9)days which has no significant difference with (23.9 ± 5.7)days in control group.The postoperative infection rate was 3.3%(2/60) which was significantly less than 20.0%(12/60) in control group(P<0.01).Conclusion Shortening drainage time can decrease the disappearance time of leakage and postoperative infection rate .