AIM: To investigate the role of angiotensin II (Ang II)and Ang II receptor (Ang II R) in the ischemic injury of kidney.METHODS: The expression of renal Ang II type 1 receptor (AT 1R) gene in renovascular hypertensive rats was detected by reverse transcriptase-polymerase chain reaction (RT-PCR), and levels of Ang II in the kidney and plasma were examined by radioimmunoassay.RESULTS: The study showed that the contents of Ang II in plasma and renal tissue in experimental group were increased signifiantly ( P

Objective: To identify the metabolites of Pulsatilla saponin D (PSD) in the intestinal microflora of rats in vitro. Methods: The sample of PSD incubated in intestinal microflora of rats in vitro was analyzed by a ultraperformance liquid chromatography/hybrid triple quadrupole linear ion trap mass spectrometry (UPLC-Q-trap-MS). The data were processed with Analyst 1.5 software, and the metabolites of PSD were confirmed by comparing the retention time and MS/MS spectra. The possible structures of metabolites were proposed by analyzing the proposed fragmentation pathway. Results: PSD (M0, parent) and seven metabolites were identified, including hederagenin 3-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinosyl (M1), hederagenin 3-O-α-L-glucopyranosyl-(1→4)-α-L-arabinosyl (M2), hederagenin (M3), hydroxylation of PSD (M4), methylation of PSD (M5 and M6), and dehydrogenation of PSD (M7). Conclusion: PSD has a wide range of metabolic in the intestinal microflora of rats in vitro.

OBJECTIVE: To identify the metabolites of Flos scabiosae extract in rats after oral administration for exploring its metabolism mode in vivo. METHODS: Welch Ultimate UHPLC C18 column (2.1 mm×100 mm, 1.7 μm) was used for the analysis, and the column temperature was maintained at 25℃. Gradient elution was conducted with mobile phase consisting of 0.1% acetic acid water (A) and acetonitrile (B) at a flowing rate of 0.35 mL·min-1. The injection volume was 6 μX. Urine, plasma, and feces samples were collected during 0-24 h or 0-12 h after oral administration of Flos scabiosae extract to rats. The samples and control samples were analyzed by UHPLC-Q-T MS/MS in the scanning mode to acquire the full-scan chromatograms. The data acquisition and processing analysis were conducted using compound retention time, precise molecular mass, elemental composition, isotopic abundance, and mass spectra with Peak View v1.2 data processing software. Metabolites were identified by comparison of chromatograms of the urine, feces and plasma samples after administraion of Flos scabiosae extract with those of the blank biological samples and the herb extract. RESULTS: The developed method is applicable to the analysis and identification of metabolites of Flos scabiosae extract in biological matrices after oral administration. Based on the investigation of Flos scabiosae extract in rats, 28 parent compounds with 31 metabolites were detected in rat urine, feces, and plasma. CONCLUSION: In this study, the developed method is simple and efficient. The main metabolism pathways of Flos scabiosae extract in rats may include oxidation, demethylation, glucuronidation, and sulfation. This study provides a novel pattern and illumination for the research of material basis of Flos scabiosae.

〔Abstract〕 The paper elaborates the definition of Biobank and the significance of constructing Biobank standardized system, through comparison of Biobank development process and experiences between China and abroad, it analyzes the existing problems and reasons of Biobank standardized system construction in China, puts forward corresponding advices.

Objective To explore the quality control method of leucodepleted red cell concentrate (LDRC) preparation ,to choice an optimal processing , in order to raise the quality of LDRC,so as to ensure the efficiency and safty for the preparation application.Methods 62 bag red cells concentrates suspensin(each 150ml,preparation from 200ml whole blood) were investigated.By linear regression multianalyzed the effects of some factors on efficiency for removal of leukocytes from red cell concentrates.Free hemoglobin value(n=8) was detected before and after leukodepleted filtration.1% samples were tested and bacterial cultured,and observed the clinical adverse effects after LDRC tranfusion.Results ⑴The plasma and platelet in red cell contentrate before filtration,the time of filtration had influence significantly on the result of leukocyte depletion.⑵The percentage of leukodepleted red cell in which residual leukocyte less than 5?10 6/unit and 1?10 6/unit were 100% and 46.9%. The percentage of leukodepleted red cell in which the leukocyte removal efficiency more than 2log(99%)and 3log(99.9%) were 100% and 56.1% .After controlling the time of filtration,the percentage of leukodepleted red cell in which residual leukocyte less than 1?10 6/unit was up from 46.9% to 90.91% and the percentage of leukodepleted red cell in which the leukocyte removal efficiency more than 3log(99.9%)/bag was up from 56.1% to 78.1%.⑶Red blood cell recovery rate was (90.48?4.73)%. There was no significant change of free hemoglublin before and after filtration.⑷No bacteria were detected by germiculture.⑸Evident side effects was no seen after transfusion.Conclusions During the course of removal of leukocytes from red cell concentrates by filtration, the sustained quality control is necessary for improving the quality of leukodepleted red cell.So enforcing aseptic manipulation rigorously and controlling the components of blood,the time of blood storage and filtration appropriately are quite important.

Objective: To optimize the extracting process of total flavonoids from Gynostemma pentaphyllum. Methods: The total flavonoids were extracted with ethanol 95% after the cellulose treatment to G. pentaphyllum, and the effects of enzyme dosage, enzymatic treatment temperature, enzymatic treatment time, and pH value on the total flavonoids yield was studied with response surface method. Results: The optimal enzymatic extracting process of total flavonoids was as follows: enzyme dosage 120 U/mL, enzymatic temperature 49 °C, enzymatic time 150 min, and pH value 3.9, and the total flavonoids yield reached 0.357%. Conclusion: Cellulase is suitable for the total flavonoid extraction from G. pentaphyllum.

OBJECTIVE: To prepare paclitaxel loaded D-α-tocopheryl polyethylene glycol 1000 succinate(TPGS)-modified carboxymethyl chitosan-rhein polymeric micelles (PTX/TPGS-CR PMs) and preliminarily evaluate their performance. METHODS: PTX/TPGS-CR PMs was prepared by dialysis method, and the preparation procedure of PTX/TPGS-CR PMs was optimized by single factor with the drug loading, encapsulation rate and particle size as the indicators, then the optimized preparation procedure was verified. The safety of PTX/TPGS-CR PMs was initially investigated by the hemolysis test and the vascular irritation test. The cytotoxicity of PTX/TPGS-CR PMs in Hela cells was studied by MTT assay. Cell uptake experiments were performed by laser confocal microscopy and flow cytometry to investigate the uptake of PTX/TPGS-CR PMs by Hela cells. RESULTS: The particle size and PDI of PTX/TPGS-CR PMs prepared by the optimized preparation were (197.3±4.4) nm and (0.131±0.021), respectively. The Zeta potential was (-31.8±0.5) mV. The drug loading and encapsulation efficiency were (48.20±3.03)% and (87.26±4.91)%, respectively. The hemolytic test results showed that the hemolysis rate was less than 1.71%. No obvious irritation was observed after intravenous injection. The cytotoxicity of PTX/TPGS-CR PMs in Hela cells was concentration-and time-dependent. Cell uptake experiments showed that PTX/TPGS-CR PMs could be efficiently uptake by Hela cells. CONCLUSION: The PTX/TPGS-CR PMs has high drug loading and encapsulation efficiency, good safety. And they exhibite slightly better antitumor activity in vitro than Taxol®.

Objective To propose a stream-media technology based on the new transmission technology. Methods Firstly,we explored the advantages and disadvantages of exiting PACS image in LAN (Local Area Network) and WAN (Wide Area Network) and then expounded distance diagnosis related to HIS and PACS,VPN technology,wireless network applications,more points consultation applied technology. At last,we made an analysis of the characteristics of the stream-media transmission technology. Results The characteristics of low bandwidth occupied,high security,and high scalability of stream-media transmission technology fit the needs of PACS development. Conclusion Application of streammedia technology transferred in PACS image provides a new economical and practical technical solution to break through limitation of network bandwidth in medical imaging integrated information,and to thoroughly make use of medical resource.

Objective To explore the efficacy of conventional physical therapy,acupuncture and hormone intervention for treating Bell's palsy so as to suggest an optimal treatment in clinical practice.Methods Clinical data on 1170 patients with Bell's palsy (BP) treated at the First Affiliated Hospital of Jinan University between October 2012 and November 2015 were collected and analyzed retrospectively.The Kruskal-Wallis test was used to compare the differences in disease course and in total cost among the patients.Seasons of the year,age,treatments,as well as the presence of immune-related diseases were the covariates studied.Multivariate logistic regression equation was used to test for any significant relationships.Results Physical therapy and hormones were found to have shortened the average course of treatment,but immune-related complicating diseases were shown to be risk factors.Patients receiving acupuncture for more than 1 month had longer courses of the disease than those without.Patients whose treatment combined hormones with physical therapy had the shortest disease course and lower total cost on average.Conclusion Early hormone intervention combined with physical therapy can shorten the course of BP and lower the cost of treatment.

Objective To detect the plasma amino-terminal pro-B-type natriuretic peptide (NT-ProBNP) of children with congenital heart disease(CHD) prior to heart surgery,to analysize its sensitivity,specificity and Youden index by receiver operating characteristic(ROC) curve,to explore the cut-off values of plasma NTProBNP for the diagnosis of congestive heart failure(CHF) in children under 5 years old before and after age stratification.Method The plasma NT-proBNP was detected for 100 children with CHD prior to heart surgery (experimental group) and 100 normal children(control group) between January 2011 to January 2013.Age stratification(0 ～ 1 year group,～3 years group and ～ 5 year group) was conducted in children with CHF younger than 5 years old to explore the cut-off values of plasma NT-ProBNP for the diagnosis of CHF.Results The NT-proBNP of 34 patients with CHF(n =100) was in accordance to Ross criteria The cut-off values were 502 ng/ L before age stratification,and they were 552 ng/L,449 ng/L,349 ng/L after age stratification for 0 ～ 1 year group,～ 3 years group and ～ 5 group.Conclusion The cut-off values of plasma NT-ProBNP for CHF were 552 ng/L、449 ng/L、349 ng/L for 0 ～ 1 year group,～ 3 years group and ～ 5 year group.