1.The analysis of blood fat and high sensitive-C reactive protein in patients with subclinical hypothyroidism
Dongmei YIN ; Min XU ; Hui TIAN
Chinese Journal of Postgraduates of Medicine 2014;37(19):19-21
Objective To analyze the change and clinical significance of blood lipid and high sensitive-C reactive protein (hs-CRP) in patients with subclinical hypothyroidism.Methods Fifty patients with subclinical hypothyroidism were selected as observation group,and 50 health people with the same gender and age were selected as control group.The blood lipid and hs-CRP levels in the two groups were measured and compared.Results The total cholesterol (TC),triacylglycerol (TG),and low density lipoprotein cholesterol (LDL-C) in observation group were significantly higher than those in control group [(5.20 ±0.84) mmol/L vs.(4.79 ±0.80) mmol/L,(2.21 ±0.97) mmol/L vs.(1.78 ±0.60) mmol/L,(3.25 ±0.65) mmol/L vs.(2.83 ±0.51) mmol/L],high density lipoprotein cholesterol (HDL-C) was significantly lower than that in control group [(1.18 ± 0.15) mmol/L vs.(1.28± 0.22) mmol/L],and there were statistical differences (P < 0.05 or < 0.01).The hs-CPR in observation group was significantly higher than that in control group [(4.2 ± 1.1) mg/L vs.(3.0 ± 0.8) mg/L],and there was statistical difference (P <0.01).Conclusions Subclinical hypothyroidism can cause increased TC,TG,LDL-C and hs-CRP and decreased HDL-C,which may accelerate atherosclerosis and cardiovascular disease.It is necessary to timely screen and treat the disease.
2.Relationship of MTHFR gene polymorphisms with infertility.
Kai-min GUO ; Run-hui TIAN ; Hong-liang WANG
National Journal of Andrology 2016;22(2):171-174
The folate metabolic pathway plays important roles in cellular physiology by participating in nucleotide synthesis, DNA repair and methylation, and maintenance and stability of the genome. Methylenetetrahydrofolate reductase (MTHFR) is a key regulatory enzyme involved in folate metabolism. Polymorphisms of MTHFR may change the level of homocysteine and affect DNA synthesis and methylation, leading to an increased oxidative stress and disturbed methylation reactions and consequently affecting reproductive function. This article presents an overview on MTHFR gene polymorphisms, proposing that multicentered, large-sample and long-term prospective studies are needed to reveal the relationship between MTHFR gene polymorphisms and infertility.
DNA
;
biosynthesis
;
DNA Methylation
;
DNA Repair
;
Folic Acid
;
metabolism
;
Homocysteine
;
metabolism
;
Humans
;
Infertility
;
enzymology
;
genetics
;
Methylenetetrahydrofolate Reductase (NADPH2)
;
genetics
;
Polymorphism, Genetic
;
Prospective Studies
3.Investigation and application of a diabetes risk score in predicting incident diabetes in male senile people in Beijing
Min LIU ; Changyu PAN ; Hui TIAN ; Mengmeng JIN
Chinese Journal of Endocrinology and Metabolism 2010;26(12):1038-1041
Objective To develop a diabetes risk score (DRS)to predict the risk of development of incident diabetes in male senile people in Beijing. Methods DRS was developed basing on a test group including a cohort of 1 370 individuals aged 48-87 years without diabetes at baseline, followed for 10 years by Logistic regression and validated on a value group including a cohort of 340 individuals aged 43-88 years without diabetes at baseline. Results The model with the highest area under the ROC curve ( AUC ) included age, hypertension,history of hyperglycemia, body mass index, fasting plasma glucose, triglycerides, and high-density lipoproteincholesterol (HDL-C). DRS was developed basing on this model with a range from 0 to 12 and an optimal cut-off of 4. AUC were respectively 0. 726 ( 95% CI0. 692-0. 759 ) and 0. 765 ( 95% CI0. 691-0. 839 ) in test group and validation group. The sum score value ≥4 had sensitivity of 65.3% and 68. 1%, specificity of 70. 0% and 64.8%, positive predictive value of 37.0% and 23.2%, negative predictive value of 88.2% and 94. 1%.Conclusion The DRS, derived from clinical information combined with plasma glucose and lipids, is an effective tool to predict incident diabetes.
4.Culture Methods of the Oligotrophic Marine Microbes
Tian TIAN ; Dong-Mei LI ; Shi-Kun DAI ; Ke-Dong YIN ; Hui-Min SUN ; Xiang LI ;
Microbiology 2008;0(07):-
Molecular methods and fluoroscopic techniques suggest that rich microbial diversity exist in the marine environment, but less than 1% of these microbes can be cultured in the laboratory conditions, and that the cultivable dominant species were even less. This limitation has long been a barrier to the development of environmental microbiology and the utilization of marine resources. In the past decade, novel methods for culture and detection of these uncultured marine microbes have successfully applied to obtain several conventionally-uncultured microbes including those from extreme environments. Those progresses have inspired researchers greatly. Developments in the research of marine microbial resources are an important basis for the study of the micro-world and deserve increasing scientific attention.
5.Relationship between Acute Exacerbation of Asthma and Respiratory Tract Infections in Children
hui-xiang, XU ; hui-yan, ZHANG ; da-tian, CHE ; min, LU
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To explore the relationship between asthma exacerbation and respiratory tract infections of viruses,Mycoplasma pneumoniae(MP),Chlamydia pneumoniae(CP)in children.Methods Seven viruses including respiratory syncytial virus(RSV),adenovirus(AdV),influenza virus A(IFVA),influenza virus B(IFVB) and parainfluenza virus Ⅰ,Ⅱ,Ⅲ(PFVⅠ,Ⅱ,Ⅲ) from the nasopharyngeal aspirate of 74 patients with asthma were rapidly diagnosed by direct immunofluorescence assay,as well as the serum MP-IgM,CP-IgM were detected by the granule agglutinating method and indirect solid-phase enzyme immunoassay(EIA),respectively.Results Pathogens were detected in 29 out of 74 cases(39.2%) with asthma exacerbation.Of whom 14 cases(43.8%) were in infant and another 15 cases(18.8%) were in preschool and school children.RSV was the leading pathogen in infant,it was discovered in 6 cases(accounting for 18.8%).The se-cond pathogen was PFVⅢ which was discovered in 4 cases(12.5%).MP,AdV and CP accounted for 6.25%,3.1%,3.1%,respectively.But in preschool and school children,MP was the most common pathogen which were discovered in 9 cases(21.4%),the following pathogen was CP which was discovered in 3 cases(7.1%),PFVⅢ and RSV only accounted for 4.8%,2.4%,respectively.There was significant differences statistically between two groups in viral respiratory tract and atypical-microorganism infections rate(Pa
6.Clinical effect analysis of primary bone graft and VSD for open and complex pilon fractures
Dawei TIAN ; Min XIONG ; Hongsheng HE ; Xiaohua HUANG ; Hui ZHAO ; Peng ZHANG
International Journal of Surgery 2014;41(8):521-524,封3
Objective To investigate the clinical effect of primary bone graft and VSD for open and complex Pilon fractures.Methods Fifty-six cases with open and complex Pilon fractures admitted in Dongfeng general hospital between January 2010 and January 2011 were treated with primary bone graft and VSD,the clinical datum were collected,for example soft tissue union,fracture union,incidence rate of traumatic arthritis,ankle article function scores.Results Fracture healed,no soft tissue infection happened,according to Helfer scores criteria,there were 22 excellent,21 good,8 fair,5 poor,excellent and good rate were 76.8%.Conclusions The method of primary bone graft and VSD resolved many questions simultaneously,for example fracture fixation,bone defect and soft tissue coverage,which has feature of simpler operation,lower infection incidence rate,better fracture union and article function than conventional methods.
7.Comparative Study of Genetic Diversity of Spatolobi caulis from Guangxi by RAPD and ISSR Method
Hui TIAN ; Changyue JIANG ; Hua ZHU ; Xiaoxun WANG ; Min ZHOU ; Jian CUI ; Yi FENG
China Pharmacy 2015;26(31):4348-4350
OBJECTIVE:To compare genetic diversity of Spatolobi caulis from different areas of Guangxi by random amplified polymorphic DNA(RAPD)and inter-simple sequence repeat(ISSR). METHODS:Through using POPGENE 32 software,Ntsys software and SPSS 17.0 software,RAPD and ISSR methods were used to study genetic diversity of 9 samples of S. caulis from dif-ferent areas of Guangxi. RESULTS:After amplification of screened 3 RAPD primers and 4 ISSR primers,and there were 198 and 315 locus,and 37 and 80 polymorphism locus. Rates of polymorphism locus were 18.7% and 25.4%;the number of effective al-leles were 1.416 8 and 1.584 0;genetic diversity index were 0.269 4 and 0.351 3;Shannon diversity index were 0.431 6 and 0.529 9. All the values of ISSR marker were higher than RAPD marker. The average genetic similarity coefficient of ISSR and RAPD were 0.757 64 and 0.683 80,indicating ISSR was more sensitive for the detection of genetic diversity. The clustering result of them was close to each other. The correlation coefficient of them were 0.847,indicating very significant positive correlation at the level of 0.001. CONCLUSIONS:ISSR could reflect more information of genetic diversity than RAPD,and is more suitable for research of genetic diversity of S. caulis from different areas of Guangxi.
8.Analysis of ten-year mortality in the male senile population with elevated fasting plasma glucose
Mengmeng JIN ; Changyu PAN ; Hui TIAN ; Min LIU ; Haiyan SU ; Juming LU ; Yiming MU
Chinese Journal of Endocrinology and Metabolism 2008;24(2):157-160
Objective To observe the all-cause and cardiocerebrovascular disease(CCVD)-related mortalities in the senile male population and the relationship of them with fasting plasma glucose (FPG) level.Methods A survey was performed among 1 572 male subjects aged 60-90.All the subjects were groups were calculated by Kaplan-Meier method and the log-rank test was used to compare the survival rates of the 4 groups.Logistic regression model was used to analyze the correlation factors of all-cause mortality and CCVD-related regression model analysis showed that age was related with the all-cause mortality.Among all these factors, age, body mass index, FPG and the history of CCVD had the relationships with the mortality of CCVD.Conclusion In the older male population, CCVD are considered as one of the main causes of death.With the increased levels of FPG, the all-cause mortality and CCVD mortality of senile male population are increased.
9.Preparation and effect of bone targeting drug of the epirubicin-oxidized dextran-alendronate compound for osteosarcoma cells
Dawei TIAN ; Hongsheng HE ; Peng ZHANG ; Xiaohua HUANG ; Hui ZHAO ; Gang LI ; Min XIONG
Journal of Chinese Physician 2014;16(11):1498-1502,1507
Objective To prepare a new bone-targeted drug of epirubicin-oxidized dextran-alendronate compound,and explore the effect of compound on osteosarcoma cells.Methods (1) Based on Schiff's base theory,we synthesized the compound.(2) We investigated physicochemical character with ultraviolet (UV),infrared (IR),and 1H-nuclear magnetic resonance (1H-NMR),molecular weight and aldehyde group content in oxidized dextran,alendronate content in oxidized dextran-alendronate compound,epirubicin drug loading capacity in the compound,and rate of charge for three matters,and affinity to bone in vitro though Hydroxyapatite (HA) absorbing test.(3) We investigated the compound's cytotoxicity effect through methyl thiazolyl tetrazolium (MTT) test,apoptosis influence through flow cytometry (FCM).Results (1) There were typical physicochemical characters.(2) Oxidized dextran molecular weight (MW) was 4 251 ± 68,number of average molecular weight (MN) was 2 551 ± 42,and molecular weight distribution width was 1.78.Aldehyde group content of oxidized dextran was (10.41 ± 0.2)mmol/mg,epirubicin drug loading capacity was (5.28 ± 0.23) %,rate of charge was 5,and 2 ~ 3 between aldehyde group content in oxidized dextran and alendronate (ALN).(3) In the MTT test,epirubicin (EPI)-Dex-ALN,EPI,ALN obviously restrained osteosarcoma cell proliferation,the minimum concentration(μg/ml) was 1,1,and 40; IC50 (μg/ml) of EPI-Dex-ALN,EPI,ALN was 0.142,0.505,0.219; 0.251,0.739,and 0.518; 45.21,27.97,and 18.96 after 24 h,48 h,72 h; in FCM (flow cytometry),apoptosis and necrosis rate of EPI-Dex-ALN,ALN,and Dex was 80.13 ±4.05,97.01 ±2.58,31.53 ±6.9,and 14.01 ±2.51.Conclusions We successfully synthesized a new bone-targeted drug delivery system,which showed better bone affinity in vivo,and kept biological activity with powerful targeted function.
10.Enhancing effect of CpG on sensitivity of Lewis lung cancer to X-ray radiation in mice
Su-Juan YUAN ; Tian-Kui QIAO ; Ji-Min SHI ; Hui-Zhong HE ;
Chinese Journal of Cancer Biotherapy 1995;0(03):-
Objective: To explore the role of cytosine-phosphate-guanine oligodeoxynucleotide(CpG ODN)in enhan- cing the radiosensitivity to X-ray in mouse with Lewis lung cancer.Methods: The tumor-bearing mouse model was in- duced by injevting Lewis lung cancer cells into the right infra-axillary dermis.Thirty-two C57BL/6J mice were evenly ran- domized into 4 groups.Group A: the control group;Group B: the X-Ray radiation group;Group C: the CpG group; Group D: the CpG plus X-Ray radiation group.Group B was treated with X-Ray radiation only(3 Gy/F,on day 1,3,5, 8,10,and 12;the total dose was 18 Gy);group C was administered with CpG ODN 0.05 mg on day 1,3,5,8,10, and 12;group D was administered with CpG ODN 6h before X-ray radiation.The tumor growth and tumor growth delay (TGD)were observed in all groups.Meanwhile,the pathological change of the tumor tissue was observed with H-E staining method and the apoptosis of tumor cells were examined with the method of TUNEL.Results: The Lewis hmg cancer-bearing model was successfolly established in mice.The tumor volumes of the treatment groups were smaller than that in lhe control group(P