Objective To investigate the therapeutic effectiveness of video-assisted thoracoscopic surgery(VATS)and thoracotomy for spontaneous pneumothorax in high altitude.Methods 256 concecutive patients diagnosed as having spontaneous pneumothorax in high altitude between July 1997 and August 2007 were analyzed retrospectively.VATS was performed in 158 cases and thoracotomy was performed in 98 cases.The operating time,blood loss during operation,chest drainage time,postoperative hospital stay,the costs of operation,the total costs of hospital stay and postoperative complication were compared between the two groups.Results The operating time in VATS group and thoracotomy group was(55.6?5.8)min and(66.3?5.1)min respectively(t=11.678,P=0.000),the blood loss during operation(40.6?5.8)ml and(120.3?6.5)ml respectively(t=11.678,P=0.000),the chest drainage time(2.6?0.7)d and(3.2?0.8)d,respectively(t =3.024,P=0.000),the postoperative hospital stay(4.8?0.9)d and(5.2?0.6)d respectively(t =4.168,P=0.000),the the costs of operation(9876?687)Renminbi Yuan and(4948?562)Renminbi Yuan respectively(t =44.532,P=0.000),the the total costs of hospital stay(17 028?956)Renminbi Yuan and(13 892?835)Renminbi Yuan respectively(t=21.743,P=0.000),the postoperative complication 12 cases and 38 cases respectively(?2 =21.351,P=0.000).The number of recurrence after operation was not statistical significant difference(?2=0.734,P=0.512).Conclusion VATS is accepted easily by patients and the total curative effect is dominant comparing to standard thoracotomy.The VATS method is the first selection for spontaneous pneumothorax in high altitude.But the defect of VATS is higher in the costs of operation and hospital stay.Decreasing applying the consumptive material of one time can reduce the costs.

This paper introduces a microtiter-plate scanning instrument for medical laboratory equipment,which is controlled by the embedded PC when moving along the X direction and Y direction on the microtiter-plate plane. The designs of the driving circuit and orienting circuit are included in this paper. This instrument has been used in PKU fluorescence laboratory equipment for the newborn baby.

Objective To observe the effect of Sijunzi Decoction (SD) on the mitochondrial DNA deletion of the aging model mice, and to explore its anti-aging biomolecular mechanism. Methods Kunming mice were divided into 6 groups according to the body weight: normal control group, .model control group, high-, middle-and low-dose SD groups (at the dose of 20, 10, 5 g·kg-1 respectively), and vitamin E group (250 mg·kg-1), 20 mice in each group.The mice models of aging were induced by D-galactose. Water maze test was used to evaluate the learning and memory ability of the aging model mice. The effect of SD on the mitochondrial DNA deletion of the aging model mice was ob-served. Results In the hippocampal mtDNA of model control group and low-dose SD group, there were about 3.87 kb fragment missing. But there was no missing pieces in high-and middle-dose SD groups. Conclusion The anti-aging biomolecular mechanism of SD is probably related to the preventive effect on the mutation and deletion of the mitochondrial DNA in the aging model mice.

Objective To explore the clinical value of high-sensitivity C-reactive protein(hsCRP) in chronic kidney disease(CKD) and the relationship between hsCRP and the different stages of CKD in children.Methods This study population consisted of patients who was diagnosised as CKD in Beijing Children′s Hospital from Mar.2007 to Feb.2008.The total number of subjects was 62 patients with 35 male and 27 female,the mean age was(8.7?4.0)years old,the course was from 3 months to 10 years.The healthy control group contains 30 healthy children with 16 male and 14 female,the mean age was(7.8?3.2) years old.The serum levels of hsCRP,creatine and ?2-microglobulin(?2-MG) were determined.The glomerular filtration rate(GFR) was caculated.The relationship between serum levels of hsCRP and ?2-MG,blood pressure as well as the renal function in these patients with CKD,stratified according to GFR were evaluated.The relationship between hsCRP and the blood pressure was evaluated.The statistical analysis for the data was done with SPSS 12.0 software.Use the t-test,order and examination and correlation analysis,P

Objective To evaluate the accuracy of CHROMagar Candida medium in identifying common Candida species .Meth‐ods Articles were extensively collected by searching the databases of MEDLINE and EMBase ,the Chinese Biomedical Database (CBM ) ,the Chinese Scientific Journals Database (CSJD) ,the Chinese Journal Full Text Database (CJFD) and through other ways . The qualities of these articles were assessed by using the quality assessment of diagnostic accuracy studies(QUADAS) .At last , summary receiver operating characteristic (SROC) curve was performed by the Meta‐Disc software ,so as to summarize diagnostic accuracy of CHROMagar Candida medium in identifying common Candida species .Results A total of 7 articles meeting all criteria were enrolled in this study .All 7 articles reported the accuracy of CHROMagar Candida medium in identifying the Candida albi‐cans ,the pooled sensitivity and specificity was 98 .3% and 98 .8% respectively ,and area under SROC curve (AUC) was 0 .998 0 .A‐mong them ,6 articles reported the accuracy of CHROMagar Candida medium in identifying Candida tropicalis ,the pooled sensitivity and specificity was 92 .5% and 99 .8% respectively ,and the AUC was 0 .998 3 .Among them ,5 articles reported the accuracy of CHROMagar Candida medium in identifying Candida Glabrata ,the pooled sensitivity and specificity was 98 .3% and 98 .7% respec‐tively ,and the AUC was 0 .996 8 .Conclusion CHROMagar Candida medium could quickly identify clinical common Candida species and results are reliable .

By taking the course of laboratory hematology in Curtin University in Australia as an ex-ample, this paper introduced the characteristics of its teaching mode, teaching contents, teaching methods, assessment methods and laboratory practice. The advantages and disadvantages of education characteristics between Australia and China were compared and analyzed on the training methods and the training objec-tives, respectively. The education characteristics of laboratory hematology in Curtin University provided an important inspiration for our education reform in the field of medical laboratory in the future.

BACKGROUND:Previous studies have reported that rapamycin can affect the proliferation, migration and adhesion abilities of endothelial progenitor cels, but there is no report on the effect of autophagy, as wel as the interaction between autophagy and apoptosis. OBJECTIVE: To observe the effect of rapamycin activated autophagy activation on the proliferation, apoptosis, and cycle of endothelial progenitor cels. METHODS:Density gradient centrifugation was used to obtain mononuclear cels from bone marrow, and the mononuclear cels were inoculated on human fibronectin-coated culture plate.Then after cultured for 7 days the adherent cels colected were the endothelial progenitor cels. Different concentrations of rapamycin (0.01, 0.1, 1 and 10 μg/L) were added and cultured for 24 hours. Western blot was used to detect the LC3-II protein expression and monitor the induction of autophagy, flow cytometry was used to observe the cel cycle progression and apoptosis changes, and methylthiazolyldiphenyl-tetrazolium bromide colorimetric assay was used to observe the proliferation ability. Meanwhile, the ultrastructural changes were observed under transmission electron microscope. RESULTS AND CONCLUSION:Compared with the control group, there was no significant increasing of LC3-II protein expression of endothelial progenitor cels in 0.01 μg/L rapamycin group, and the LC3-II protein expression was in the high level. The LC3-IIprotein expression in the 1 μg/L and 10 μg/L rapamycin groups was higher than that in the control group, but lower than that in the 0.01 μg/L rapamycin group, which indicated that autophagywas particularly active when the concentration of rapamycin was 0.01 μg/L. The apoptosis of endothelial progenitor cels was increased with the increasing of concentration of rapamycin, and the proliferation rate was decreased with the increasing of concentration of rapamycin. The results indicate that activation of autophagy by bapamycin can promote the cel apoptosis, change the cel cycle significantly, and can inhibit the proliferation of endothelial progenitor cels.

Objective To compare the effect of Sijunzi Tang and Yougui Yin on learning-memory ability and free radicals in aging rat model.Methods The aging rat model was induced by intraperitoneal injection of D-galactose.The swimming time and error times in water maze test were observed and the free radicals SOD,MDA,NO,NOS in rat brain tissue were measured.Results Sijunzi Tang could shorten the rat swimming time in water maze test.Yougui Yin could increase SOD level and decrease MDA level in brain tissue of model rat,while Sijunzi Tang could decrease NO and NOS level.Conclusion Sijunzi Tang could improve the rat spatial learning and memory ability,and Yougui Yin could relieve the damage of free radical in brain tissue of the aging rat model.

Objective To observe the effects of polysaccharide extracted from Yangwei Mixture on immunologic functions in mice.Method Immuno-suppressant mice model was established by intraperitoneal injection of CTX.The effects of the polysaccharide were observed through phagocytosis test of mice celiac macrophagocyte and serum hemolysin level test as well as lymphocyte transformation test.Result The polysaccharide can obviously increase the percentage of phagocytosis of mice celiac macrophagocyte,accelerate the formation of hemolysin and promote the lymphocyte transformation.Conclusion Polysaccharide extracted from Yangwei Mixture is a good immuno-stimulant,and can counteract the immuno-suppression induced by CTX.

Objective To investigate the feasibility of fast track surgery (FTS) application in arthroscopic reconstruction of anterior cruciate ligament.Methods A total of 80 cases of anterior cruciate ligament(ACL) tear patients were randomized into two groups.While the experimental group (n =42) was treated with the conception of FTS, and the control group(n =38) was treated with the traditional methods.The postoperative pain, knee Lysholm score, quality of life score (SF-36), knee joint activity (EUROPEP), and postoperative infection were compared between the two groups.Result The VAS score of post-operation pain (VAS) 1 h(1.80±1.13) ,6 h(1.91±0.98), 12 h(1.73±0.95) ,24 h(1.68±0.65) ,48 h(1.69±0.90) ,the time stay in hospital (7 ± 1), total cast (205 600 ± 7 600) yuan, were significantly decreased compared to the traditional therapy group(VAS : (7.00± 1.56), (7.41±0.93), (6.82±1.12), (6.65±0.76), (6.34±0.88) ,P ＜0.05 or P＜0.01;(12± 1) d, P =0.021;(28 600±5 400) yuan, P =0.042).Recovery time of ROM of experimental group was significant lower than control group (P ＜ 0.05).But the comparison of patients, satisfaction of post-operation in two groups was inverse(P=0.007).The Lycholm scores of post-operation of FTS group were remarkably higher than the control group and the scores of prior treatment(85.1 ± 14.2) vs (73.6 ±12.3);P＜ 0.05).Conclusion The new methods of FTS can apparently accelerates recovery after ACL reconstruction,reduces complications, shorten time stay in hospital, cut down the total cost, and improve the experience and the satisfaction of patients.It was safety and feasible.