Objective:To establish a method for the determination of protocatechuic acid, protocatechuic, catechin, epicatechin and rutin in Begonia grandis Dry. Methods:The 5 active ingredients in the samples were extracted by 70% methanol and analyzed by ultra performance liquid chromatography (UPLC). Chromatographic separation was performed on a Waters ACQUITY HSS T3(2. 1 mm × 100 mm, 1. 8 μm) column with gradient elution, and the mobile phase was solvent A: acetonitrile and solvent B:0. 2% glacial acetic acid (0-12 min:A:5→25 and B:95→75;12-30 min:A:25→50 and B:75→45). The effluent was monitored by a VWD detector set at 280 nm. The flow rate was set at 0. 2ml/min and the injection volume was 1μl. The column temperature was maintained at 30℃. Results:The calibration curves were all linear (r≥0. 99) over the test ranges. The average recovery was 96. 92%-99. 75%(n=6). Conclusion:The established method can rapidly obtain an accurate and reproducible result, which can be used to control the quality of Begonia grandis Dry.

OBJECTIVE:To establish a method for simultaneous determination of vicexin 2,vicexin 1 and schaftoside in Shilin-tong tablet. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase of methanol-0.1% phosphoric acid (gradient elution)at the flow rate of 0.8 ml/min,the detection wavelength was 334 nm,the column temperature was 25 ℃, and the injection volume was 10 μl. RESULTS:The linear range was 0.31-3.10 μg for vicexin 2 (r=0.9999),0.28-2.80 μg for vicexin 1(r=0.9999)and 0.39-3.90 μg for schaftoside(r=0.9999),respectively;RSDs of precision,stability and reproducibili-ty tests were lower than 3.0%;recoveries were 95.2%-100.4%(RSD=1.6%,n=9),96.3%-100.8%(RSD=1.4%,n=9) and 95.7%-100.9%(RSD=1.7%,n=9),respectively. CONCLUSIONS:The method is simple,sensitive ,accurate,reliable and repro-ducible,and can be used for the quality control of Shilintong tablet.

Audiology ; China ; Congresses as Topic

A questionnaire survey was conducted among 319 mental health workers from 319 grassroots health institutions of 21 districts (cities,counties) in Chengdu in March 2013.The items of survey included general information of participants,subjective perceptions and status of community mental health services.Total 252 effective questionnaires were received,the average age of participants was (33.12 ± 9.39) years (19-61);59.5％ had clinical medicine and 16.3％ had public health background;most of them had educational levels of three-year college or below (84.9％);the average duration of working experience was 18 months;86.5％ was part-time;most of participants (87.4％) thought that the mental health service was a hard job and only 25.3％ expressed satisfaction with the work.Only 16.3％ of community health service centers had rehabilitation facilities for mental illness patients.Public health education was carried out for average (2.21 ± 1.62) times/year,the education for mental patients and family members was (1.62 ± 1.83) times/year.Between areas with sufficient detection rate of mental illness and those with insufficient detection rate,there was significant difference in training frequency of mental workers (P =0.012),there were no significant differences in having formal employment of mental workers (P =0.064),professional guidance,co-working with police officers,co-working with Disabled Persons' Federation,and the duration of working experience (all P ＞ 0.05).The results suggest that the capacity of mental health service in community levels is relatively weak in Chengdu,and the personnel training,public education and rehabilitation work of mental health need to be strengthened.

AIM: To investigate effect of autogenous conjunctival flap transplantation in the treatment of severe fungal corneal ulcer.
METHODS: A total of 83 cases ( 83 eyes ) with severe fungal corneal ulcer treated in our hospital from Jan. 2013 to Jan. 2016 were selected. Among them, 36 cases ( 36 eyes) were treated with drug therapy, and 47 patients(47 eyes ) were treated with autogenous conjunctival flap transplantation, two group did fungal culture and identification, observed the two groups treatment effect.RESULTS:The effective rate of surgical treatment group was 89%, significantly higher than that of drug treatment group, the difference was statistically significant ( P <0.05 ); The effective rate of surgical treatment of filamentous bacteria was 92%, which was significantly higher than that of drug treatment, the difference was statistically significant(P<0. 05). The effective rates of surgical treatment and drug treatment of yeast were 80%and 78%, the difference was not statistically significant( P<0. 05).
CONCLUSION: Autologous conjunctival flap transplantation in the treatment of severe fungal corneal ulcer has good curative effect. The operation is simple, worthy of recommendation method. Filamentous bacterial corneal ulcer without good drug therapy effect, surgical treatment should be performed in a timely manner to improve the curative effect.

S100A4, a member of S100 superfamily of Ca~(2+)-binding proteins, is a polypeptide containing 101 amino acids. S100A4, which is overexpressed in most tumor cells, plays pivotal roles in growth, invasion and migration of tumor by regulating cell cycle progression, differentiation, apoptosis, invasion and metastasis of cells. This article reviews S100A4′s structure, function and potential application as the new target in the therapy of tumors.

Burns ; complications ; therapy ; Cicatrix, Hypertrophic ; therapy ; Humans ; Keloid ; therapy

Audiology ; China ; Congresses as Topic

Recently, it is reported that regulatory T cells (Tregs) can be reprogrammed into a novel population [interleu?kin (IL)-17+Foxp3+T cells] phenotypically and functionally resembling Th17 cells under complicated cytokine circumstanc?es. IL-17+Foxp3+T cells are characterized by production of IL-17 and expression of retinoic acid receptor related orphan re?ceptor (ROR)γt, demonstrating dual functions in immune response and providing novel insight into the interconnection be?tween Tregs and Th17 cells. In this review, we lay emphasis on the phenotype features, origination, differentiation and the pleiotropic functions of IL-17+Foxp3+T cells. Furthermore, we summarized the functions of IL-17+Foxp3+T cells in inflam?matory disease and tumor microenvironment.

AlM:To discuss the application of prism alternate cover test ( PACT) and perimeter strabismometry in strabismic operation.METHODS: A total of 145 patients with concomitant esotropia or exotropia, aging 2 ~ 50 years old, were included. They were examined by PACT and perimeter strabismometry before operation. The strabismic operations were performed, the orthotropia rate of postoperation was assessed.RESULTS:Using PACT and perimeter strabismometry, there was a significantly difference of deviating angle ( P<0. 05) in mild and severe strabismic group. But there was no significantly difference of deviating angle (P>0. 05) in moderate strabismic group. The functional cure rate of postoperation was respectively 70. 83%, 67. 01%; the clinical cure rate was 20. 84%, 24. 74%; invalid rate was 8. 33%, 8. 25% .CONCLUSlON: The application of PACT and perimeter strabismometry in strabismic operation design, referring to the eye axial length, this can be helpful of improving the cure rate postoperation and decreasing the danger of reopreation.