Objective: To investigate the effects of alcohol, chitosan、 101 juice clarifying agent and ZTC natural clarifying agent on content of active components and the solid compositions in zhonglizhikang decoction. Methods: The solid compositions of different samples were determined by the methods adopted in pharmacopeia, and the chrysophanol content was determined by HPLC. Results: Chitosan has no effect on the content of chrysophanol, and solid compositions decreased by 30.8%. But alcohol、 101 juice clarifying agent and ZTC natural clayifying agent can decrease solid compositions and the content of chrysophanol. Conclusion: Chitosan can decrease solid compositions but has no effect on the content of active components.

Objective To construct and identify the transgenic plant vector recombinant pBI-Eg95 plasmid of Echinococcus granulosus. Methods Total RNA was extracted from hydatid cyst protoscoleces of Echinococcus granulosus after sonication. A couple of specific primers were designed on the basis of known sequences of Eg95 gene. The desired gene was amplified by PCR technique from the cDNA, and then was cloned into the plant expression vector pBI121 to construct the recombinant pBI-Eg95 plasmid. The recombinant plasmid was electroporated into Agrobocterium tumefaciens (At) LBA4404 strain. The positive recombinant clones were confirmed by restriction endonuclease digestion and characterized by PCR. Results For RT-PCR, a specific band around 471 bp was amplified. The result of DNA sequencing of Eg95 showed the identity with the published sequence. The same band was obtained by restriction endonuclease digestion and PER from the plasmids of positive recombinant At(rAt). Conclusions The recombinant pBI-Eg95 plasmid was successfully constructed, and it provides the basis to further research of the transgenic plant vaccine of Echinococcus granulosus.

Objective To measure the levels of serum transferrin receptors (sTfR) and serum ferritin (SF) in children with hemoglobin H disease for investigation the iron metabolism(sideropenic or iron overload) and to guide the proper intervention.To get a best index of judging the iron overload in HbH children by analysis of the values of sTfR and sTfR/lgSF.Methods Peripheral blood specimens were obtained from 50 cases of HbH children and 30 cases of healthy normal controls,the levels of sTfR and SF were examined by enzyme linked immunosorbent assays(ELISA).The diagnosis efficiency of sTfR and sTfR/lgSF in HbH children′s iron metabolism was judged by receiver operator cha-racteristic (ROC) curve analysis.Results The level of sTfR in HbH group was (6.74?1.02) mg?L-1,which was significantly lower than that in healthy control group[(8.09?0.67) mg?L-1](P

A catalytic kinetic photometric method for the determination of nitrite with surfactant sensitizer has been developed. The method is based on the catalysis of NO2- to the discoloring reaction of pyronine B oxidized by potassium bromate in the solution of dilute H3PO4. When cetylpyridine bromide was used as a sensitizer of the reaction, the sensitivity increased by 2.5 times approximately. There was linear relationshop of lg(Ao/A) with nitrite in the range 0.005 ～ 0.15 mg/L. The detection limit was 0.004 mg/L. The method was used in the determination of trace nitrite in water and vegetable samples with satisfactory results.

Cell Proliferation ; Cell Transformation, Neoplastic ; Epigenesis, Genetic ; Hodgkin Disease ; genetics ; immunology ; metabolism ; pathology ; Humans ; Immunophenotyping ; NF-kappa B ; metabolism ; Receptor, Notch1 ; metabolism ; Reed-Sternberg Cells ; immunology ; metabolism ; pathology ; Transcription Factor AP-1 ; metabolism

Objective To optimize the extraction process of total flavonoids in Hypericum perforatum L.. Methods The concentration and volume of alcohol, extraction time and frequency were systematically studied by the total flavonoids as the marker. Results The more effective way to extract total flavones was extraction with 8 times amount of 70% alcohol under reflux for 3 times and 2 h for each time. Conclusion This method obtains more effective components. It is stable and reliable.

The public welfare of public hospitals is determined by the social attributes of its public sector,which is a manifestation of public welfare in health management.The public welfare of public hospitals is conceptual,which is existed by the specific form,thus affects people's awareness about the public welfare in public hospitals.The paper introduced the concept of public welfare of public hospitals,analyzed the characteristics of public welfare in public hospitals,and summarized the manifestations of public welfare and the intervention policies on public welfare in public hospitals since the reform of public hospitals in China.Then the paper put forward the suggestions on the development path of public welfare in public hospitals.

OBJECTIVE: To provide reference for drugstores on the promotion of marketing of drugs.METHODS: Through market research,the principle,the advantageous position and the details for drugs display were analyzed.RESULTS & CONCLUSIONS: The display of drugs was correlated to their marketing.The marketing terminal of drugs should pay attention to the art of drugs display.

Objective:To investigate the dynamic change of tetramethylpyrazine (TMP) in processing of Chuangxiongchatiao Granules. Methods: To explore the influences of the different extraction, concentration and drying method on the transferring rate of TMP by HPLC. Results: Transferring rate of TMP is higher by alcohol than by water. The loss of TMP in concentrating process is lower by rotating distillation than by direct heating concentration and by reduced pressure concentration. The transferring rate of TMP is higher by reduced pressure desiccation than by ordinary pressure. Conclusion: The better extrating way of Chuangxiong is by alcohol in the processing of Chuangxiongchatiao Granules; the concentrating and drying process should be done at the low temperature and by rapid dry process.

Colitis, Ulcerative ; Humans ; Infant, Newborn ; Male