ObjectiveTo investigate the effects of recombinant human erythropoietin (rhEPO) on the apoptosis of human mesenchymal stem cells induced by H2O2 and related cell signaling pathways.Methods After being cultured in vitro, human MSCs were treated with rhEPO.Phosphorylation of ERK1/2,p38 MAPK and PI3K/Akt was detected by using Western blotting.One h after pretreatment with1U/ml rhEPO,cells were cultured in the presence of1mmol/L H2O2 for1h.Cell morphology was observed under the inverted microscopy.Cell apoptosis was determined by using flow cytometry,migration assay was performed in Transwell chambers,and adhesion assay was performed by plastic dishes.ResultsRhEPO could increase phosphorylation of PI3K/Akt pathway in human MSCs,but reduce phosphorylation of p38MAPK.RhEPO had no obvious effect on ERK1/2 pathway and total proteins of Akt and p38MAPK.RhEPO could decrease apoptosis of human MSCs induced by H2O2 (P＜0.01) and the inhibitory effect was abrogated by Ly294002 but not anisomysin.Conclusion RhEPO could protect MSCs from apoptosis. Activation of PI3K/Akt pathway was involved in the effect of rhEPO on apoptosis.

OBJECTIVE To investigate the prevalence of metallo-?-lactamase in carbapenem-resistant clinical Pseudomonas aeruginosa isolates.METHODS Sixteen strains of P.aeruginosa isolated from Tianjin General Hospital during 2005-2006 which were resistant to all tested antibiotics besides carbapenem were studied.The general PCR was adopted to amplify the resistance genes of matallo-?-lactamae.Restriction endonucleases performed restriction analysis were used to certify the type of genes of matallo-?-lactamase by different endonuclease recognition site.RESULTS Two of the 16 clinical strains of P.aeruginosa(named P02 and P16) were confirmed bearing IMP-1 metallo-?-lactamase gene detected by PCR amplification and restriction analysis.CONCLUSIONS P.aeruginosa producing IMP-1 metallo-?-lactamase is the major type in our hospital.The hydrolyzing effect of the metallo-?-lactamase is not the main machanism in these carbapenem-resistant P.aeruginosa isolates.

Objective To determine the genotypes of human papillomavirus (HPV) and to estimate their recurrence of condyloma acuminatum (CA) among female in Xi'an.Methods HPV genotype was detected with human papilloma virus genotyping kit in 63 female patients with CA.Follow-up was carried out to observe the recurrence of CA in these patients after CO2 laser treatment.Results Of the 63 specimens,61 (96.83％) were positive for HPV,and a total of 16 HPV subtypes were identified.The predominant genotypes were HPV6 (59.2％),11 (22.95％),42 (8.20％),43 (8.20％),52 (8.20％),16 (6.56％) and 18 (4.92％) respectively.43 cases of single HPV infection was accounted for 70.49％ (43/61),mixed infection in 14 cases was accounted 22.95 ％ (14/61).61 cases of patients with laser treatment,observing the recurrence of cuses after 3-month later,it was found that the recurrence rate in higher risk group and mixed infection group was higher than that in low-risk group.Conclusion HPV6,11 is main HPV infection type of the female patients in Xi'an,the recurrence of CA is higher in patients with high risk HPV than in those with low risk HPV and mixted infection.Mixted infection of HPV and high risk HPV is a risk factor for recurrence of female genital warts in Xi'an region.

Dysarthria ; etiology ; Encephalitis, Viral ; complications ; Facial Paralysis ; etiology ; Humans ; Syndrome

OBJECTIVETo investigate the reproducibility of Gleason scores for prostate cancer.

METHODSBased on the revised Gleason Scoring System of the International Society of Urological Pathology ( ISUP) , we analyzed the reproducibility and difference of Gleason scores in 49 cases of prostate cancer using the methods of combination and grouping.

RESULTSThe total reproducibility of Gleason scores among 15 pathologists was good (κ = 0.642), 62.2% by the combination method, the highest in Gleason 5 + 5 (81.2%) and 5 +4 (73.3%), then in Gleason 4 + 4 (67.5%), 3 + 3 (64.0%), 4 +3 (61.3%), and 3 + 4 (44.0%), and the lowest in Gleason 4 + 5 (38.9%) and 3 + 5 (33.3%). The total reproducibility of Gleason scores by the grouping method was 71.4%, the highest in Gleason 9-10 (84.9%) , then in Gleason 7 (76.7%) and 6 (64.0%), and the lowest in Gleason 8 (60.7%).

CONCLUSIONThe reproducibility of Gleason scores remains to be further improved in prostate cancer, mainly concerning the understanding of Gleason 3 and 4 carcinoma.

Aims To establish several behavioral paradigms to characterize the psychotropic effects of hallucinogens which ze-brafish was utilized as a model animal, and then to investigate the effects of potent hallucinogenic drugs on these models. Methods With the video record and track system, the behavior was recorded and quantified automatically. In the experiments, the bottom dwelling test, social behavior and mirror test were performed to test the hallucinogenic effects of drugs. Metham-phetamine (METH, 2 mg·L-1) and ketamine (20 mg·L-1) were selected as experimental challenges. The 30 min pre-treat-ment time was chosen based on our prior experience in zebrafish models. Results Compared to the normal group, in dwelling test, acute exposure of zebrafish to METH and ketamine de-creased transitions significantly, and in mirror reflection test, the drug-treated fish changed the preference for mirror zone, and ex-hibited aggressive for their mirror images. The pretreatment of METH and ketamine significantly reduced the contact durations, and the ketamine inhibited the contact frequency each other, the results indicated that the social interaction of zebrafish was im-paired. Conclusion The results confirm high sensitivity of ze-brafish models to hallucinogenic compounds with complex behav-ioral and physiological effects.

Objective To investigate the dynamic changes in brain natriuretic peptide (BNP) and the outcome of patients with chronic congestive heart failure (CHF) after the treatment with metoprolol. Methods Sixty-five patients with CHF were admitted to the Forth Affiliated Hospital of Zhengzhou University during April 2004 to April 2007, and they fulfilled the diagnostic criteria of chronic CHF issued by New York Heart Association (NYHA). Of all the patients the left ventricular end-diastolic dimension (LVEDD) ≥65mm. All the patients were randomly assigned into metoprolol group (34 cases) and control group (31 cases), and received conventional therapies [rest, reduced sodium intake, administration of digitalis, diuretic, angiotensin-converting enzyme inhibitor (ACEI) and aldosterone antagonists, etc.]. The patients in metoprolol group were given metoprolol (6.25mg as initiative dose and dose was increased according to illness). NYHA functional classitication, cardiothoracic ratio on chest X-ray film, cardiac output (CO), cardiac index (CI), left ventricular ejection fraction (EF) and LVEDD in echocardiogram were observed, and the plasma BNP level was determined with an immunoradiometric assay (IRMA). Results After 5 months of treatment, the clinical symptoms of patients were improved obviously and p1asma BNP level was significantly lowered in both groups, while p1asma BNP level was significantly lower in metoprolol group compared with control group (200.6?12.1 vs 120.7?14.1, P

Objective To investigate the distribution and expression of adiponectin receptors in different tissues of normal Wistar rats. Methods The rats were euthanized and the total RNA was extracted from equal weight tissue samples of cardiac muscle, skeletal muscle, liver, kidney, testicle and fat. RT-PCR was employed to amplify the isolated cDNA, and the expression of adiponectin receptors in different tissues was semi-quantitatively analyzed. Results AdipoR1 was observed in rat cardiac muscle, skeletal muscle, liver, kidney, testicle and fat, with the highest expression in testicle and fat (P

Objective To study MRI characteristics of viral encephalitis and the possibility of differentiating diagnosis with acute disseminated encephalomyelitis(ADEM).Methods MRI findings in 56 patients with viral encephalitis and 50 patients with ADEM were analysed and emphasized on MR findings of viral encephalitis.Results The abnormal signal lesions were found in 56 patients with viral encephalitis,including multiple or single,symmetrical or asymmetrical large patch shape lesions,the lesions located mainly in cortices,subcortical and basal ganglia and thalami.The lesions appeared as long T_1 and long T_2 on MRI.Enhanced MRI was performed in 27 cases,and demonstrated abnormal enhancement in 17 cases,including large patch or gyrus shape enhancement in 10 cases.Multiple and asymmetrical spot-patch white matter lesions were revealed in the frontal lobe,parietal lobe,occipital lobe,temporal lobe,and periventricular areas with ADEM.The lesions were long T_1 and long T_2 on MRI.Enhanced MRI was performed in 20 cases,and demonstrated abnormal enhancement in 15 cases,peripheral ring-type or patch shape enhancement was observed in all cases.Conclusion MRI is one of the important methods in diagnosis of viral encephalitis,it can provide evidences for early diagnosis and differentiation in the certain degree.