China Journal Full-text Database (CJFD) was undertaken to identify relevant articles. This article aims to classify data on ossicular replacement prosthesis (ORP) and to analyze the application of different kinds of ORP to auditory rehabilitation. Studies demonstrated that different ORP had different advantage or disadvantage. Autologous cartilage is convenient to obtain and difficult to absorb such as antilobium cartilage, nasal septum cartilage and so on. But degeneration and absorption are detected in a long-term. Homograft tooth with good histocompatibility, tooth sound conduction, weak rejection and high stability is easy to collect, to store for a long time, and to apply in clinic, as well as without organic matter after disposing. However, this kind of tooth is easy to move and cannot be used by most physicians. Titanium ORP possesses good biocompatibility and histocompatibility, avirulence, corrosion resistant properties, light mass, and is hard to dislocate, can firmly connect to the stirrup bone, resulting in a postoperative significant outcome. Bioceramic ORP is widely used in ear surgery, because it has inorganic salt component, good biocompatibility, and low dislocation rate. With deep studies on biomaterials and discovery of new materials, many scholars have designed a compound auditory ossicle to combine the predominance of various materials and to avoid their shortcomings.

This article analyzed both the etiopathogenisis and high risk factors of DVT in patients of orthopaedics diseases. Furthermore, it explained both the prophylaxis and therapies of DVT from traditional Chinese medicine (TCM),western medicine, and combined traditional Chinese and western medicine point of view.

Objective To develop a new intelligent medicine chest to update medical prescription and select medicine automatically based on the patient's requirements.Methods Information interaction was realized with Bluetooth and the connection between Java-based Yiyi health management app and a PLC module of the chest,so that auto dressing,medicine sorting and distribution could be executed according to medical prescription.The medicine chest was composed of PLC,constant-speed motor,sheave,lead rail mechanism,guide slot for medicine distribution,base,stricking mechanism and pushpull electromagnet.Results The chest eliminated medicine abuse,missed use or excessire use of the elderly.Conclusion The chest gains advantages in structure and function,and thus is worthy promoting practically.

Objective:To evaluate the application status and change trends of insulin and its analogs in Nanjing area during 2011 to 2013. Methods:The varieties, sales amount and DDDs of insulin and its analogs in 43 hospitals of Nanjing during 2011 to 2013 were analyzed. Results:The use of insulin and its analogs was increased year by year from 2011 to 2013 with the average annual growth rate of 16%. Glargine, pre-mixed insulin 30R, aspart 30, human insulin and aspart ranked the top five in the sum of money with the proportion above 80%. Pre-mixed insulin 30R, aspart 30, glargine, human insulin and common insulin ranked the top 5 in DDDs. Conclusion:The application of insulin and its analogs is wide in clinics, and the usage and sum of money both show rapid growth trend.

Objective To make the clinical evaluation of Targis/Vectris restorations. Methods 337 cases with Targis/Vectris restorations were rechecked 12～50 months after cementation respectively. The restorations were evaluated clinically according to the USPHS standard. Results The changes of Targis/Vectris restorations in colour match, boundary line, wear and tear, marginal adaptation and surface grain were not obvious within 12～50 months. The secondary caries were not found. The successful rate of the restorations was 95.4 %.Conclusion The clinical prosthetic results of Targis/Vectris restorations are reliable.

Objective To explore the effects and mechanisms of urokinase-type plasminogen activator (uPA) on high glucose-induced rat mesangial cells proliferation and phenotype transformation. Methods Rat mesangial cells were cultured and incubated in media containing either 5 mmol/L D-glucose or 30 mmol/L D-glucose with or without addition of wortmannin, or uPA (105 U/L) for different time periods. At the end of the incubation period, mesangial cells proliferation was assessed by MTT assay and flow cytometric analysis. Cyclin-dependent kinase 2 (CDK2) and p27kip1 expression and activation of Akt were evaluated by Western blotting and Akt kinase assay respectively. Furthermore, the expression and distribution of α-SMA were detected with laser confocal microscopy. Results MTT assay and flow cytometric analysis demonstrated that high glucose induced mesangial cells proliferation (P＜0.05) and an incresed proportion of cells in G2/M+S stage after 24 h incubation (P＜0.01), which were attenuated by uPA or wortmannin (P＜0.01). High glucose induced the enhance of Akt activity after 3 h (P＜0.05), and the effect was inhibited by wortmannin or uPA (P＜0.01). High glucose did not alter CDK2 expression (P＞0.05),but significantly inhibited p27kip1 expression (P＜0.05), which was attenuated by wortmannin or uPA (P＜0.01). High glucose induced the up-regulation of α-SMA expression and perinucleus location in mesangial cells after 24 h (P＜0.01), which were alleviated by wortmannin or uPA (P＜0.01). Conclusion uPA up-regulates p27kip1 expression and counteracts high glucose-induced mesangial cells proliferation and phenotype transformation via blocking PI3K-Akt signaling pathway.

Objective How to avoid the rejection of the synthetic patch and human tissue has become an urgent problem to be solved.The article investigated thein vivobiocompatibility of polypropylene mesh scaffold with adipose -derived stem cells(ADSCs) in rabbits. Methods Rabbit ADSC suspension were prepared.ADSCs were seeded onto polypropylene mesh scaffolds after passage and amplification and cultured invitro for 1 week .The polypropylene mesh and ADSC fixed polypropylene mesh were implanted respec-tively into the surface of rectus abdominis in rabbits.4 weeks later, adhesion and erosion of the meshes were evaluated, HE staining was used in histological observation and RT-PCR was applied to detect the dynamic changes of VEGF mRNA level.ADSCs were isola-ted from rabbit subcutaneous adipose tissue after collagenase digesting, filtrating and centrifuging. Results The results of flow cy-tometry showed that the expressions of CD44, CD73, CD90, CD45, CD14 and CD34 were 98.54%, 95.32%, 98.49%, 1.21%, 3.01%, 2.14%, respectively.Polypropylene mesh, ADSC-fixed polypropylene mesh had different degrees of corrosion and adhesion , but polypropylene mesh showed denser adhesion.In comparison with polypropylene,ADSC fixed polypropylene meshes induced a mil -der chronic inflammation response,with lower scores for inflammation (1.1 ±0.2 vs 0.6 ±0.1, P=0.001), higher scores for neovas-cularization (17.0 ±0.0 vs 2.6 ±0.3, P=0.000) and fibroblastic proliferation(0.9 ±0.1 vs 2.2 ±0.2, P=0.001).Relative a-mounts of VEGF mRNA of were significantly lower for ADSC-fixed polypropylene compared with the remaining polypropylene meshes (t=94.6, P<0.05). Conclusi on Polypropylene mesh scaffold with ADSCs exhibits excellent cellular compatibility and have a bright future in clinical practice.

Objective: To investigate the expression of p53 protein in the adaptable reaction of EL-4 cells induced by low dose ionizing radiation (LDR), which may provide the experimental clues for studying the repair mechanism of DNA damage in the adaptable reaction of ELA cells induced by LDR. Methods: EL-4 cells were divided into control group, radiation groups (the radiation dosage was 1, 2, and 3 Gy), and adaptable radiation groups (75 mGy + 1 Gy, 75 mGy + 2 Gy, and 75 mGy + 3 Gy). The p53 mRNA and protein expression in EL-4 cells was measured by flow cytometry and RT-PCR methods, respectively. Results: The expression levels of p53 protein in the three adaptive radiation groups were significantly higher than the normal control group (P<0.01). Adaptive radiation at 75 mGy induced adaptable reaction of EL-4 cells and decreased the expression level of p53 protein compared with control group (P<0.01). The expression level of p53 mRNA showed the same change as its protein level in EL-4 cells. Conclusion: The expression level of p53 protein is decreased in the adaptive radiation group, which suggests that p53 might play important roles in the adaptable reaction of EL-4 cells induced by LDR.

Objective: To study the color in ShoFu colormeter plate. Methods: The values of color degree of the cervical, body and incisal end of various teeth in the ShoFu colormeter plate were measured by digital camera and analyzed by computer using CIE1976L*a*b* system and JISZ8722 standard. Results: In ShoFu plate the brightness in the cervical, body and incisal end of various teeth changed following a certain rule, the brightness in group R and VR were higher than that in group A. While the values of color degree in other groups changed without any rule. Conclusion: The digital camera and computer may be used in dental color study.

A patient with basidiobolomycosis is reported,the clinical course described,the ultra- structural and histologic changes observed,and mycologic examination was made.The results indicated that:(1)In the course of using corticosteroids,because the inflammatory reaction was suppressed par- tially,the clinical manifestation was relatively relieved.In the loci the pathogenic fungi still grew well, and focal dissemination was observed;(2) In the course of using itraconazole,the drug exerted fungistatic and fungicidal action through the destruction of cell membranes of fungi.Meanwhile,itra- conazole also exerted a synergistic action with the defense function of the human body,resulting in an enhanced therapeutic effects.The strong acute inflammatory reaction was triggered by the destroyed fungi caused by itraconazole.Then the fungi were cleared,the lesions were absorbed and repaired.Sur- gical intervention may be helpful to remove abscesses and fibrous nodules formed in the clinical course, so as to shorten the course of treatment.