1.The therapeutic efficacy of minimally invasive modified transforaminal lumbar interbody fusion combined with unilateral nail holding system in patients with extreme lateral lumbar disc herniation
Zhenpeng GUO ; Hui SHANG ; Bingkui LI ; Xiaopeng GUO ; Biwen CENG ; Lei DENG
Chinese Journal of Postgraduates of Medicine 2015;38(12):905-908
Objective To investigate the therapeutic efficacy of minimally invasive modified transforaminal lumbar interbody fusion (TLIF) combined with unilateral nail holding system in patients with extreme lateral lumbar disc herniation.Methods Thirty-eight patients with extreme lateral lumbar disc herniation were divided into minimally invasive modified TLIF combined with unilateral nail holding system group (observation group) and conventional open TLIF group (control group) with 19 cases each according to random digital table method.The therapeutic efficacy was compared between 2 groups.Results Surgery was completed successfully in 2 groups,and there was no perioperative complications.The amount of bleeding and hospital stay length in observation group were significantly less than those in control group:(152 ± 82) ml vs.(258 ± 104) ml and (6.5 ± 2.3) d vs.(12.7 ± 3.6) d,and the rate of fusion and score of Japan Orthopaedic Association 6 months after surgery in observation group were significantly higher than those in control group:18/19 vs.10/19 and (14.5 ± 2.1) scores vs.(12.6 ± 1.9) scores.There were statistical differences (P < 0.01).There was no statistical difference in complications between 2 groups (P > 0.05).Conclusion Minimally invasive modified TLIF combined with unilateral nail holding system in patients with extreme lateral lumbar disc herniation has less blood loss and shorter hospital stay,and it is effective and safe.
2.Anti-miR-145 promotes human airway smooth muscle cell proliferation and osteopontin synthesis in vitro.
Peifen CHEN ; Zhihui QIU ; Guohua HUANG ; Xiangmei ZHANG ; Wujian PENG ; Hui CENG ; Wenyan LAI
Journal of Southern Medical University 2015;35(7):1073-1075
OBJECTIVETo investigate the effect of anti-miR-145 on human airway smooth muscle cell (HASMC) proliferation and osteopontin systhesis in vitro and explore the mechanisms.
METHODSHASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes in osteopontin synthesis after the treatment was quantified with Western blotting.
RESULTSTreatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis (P<0.01).
CONCLUSIONAnti-miR-145 promotes HASMC proliferation and osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis of airway remodeling.
Airway Remodeling ; Apoptosis ; Cell Proliferation ; Cells, Cultured ; Humans ; MicroRNAs ; antagonists & inhibitors ; Myocytes, Smooth Muscle ; drug effects ; Osteopontin ; biosynthesis ; Respiratory System ; cytology
3.High Expression, Purification and Immuno-indentification of Recombinant HIV Surface Antigen gp120
Chen, CHEN ; Kun, LIU ; Jin-zhong, WANG ; Ming, MA ; Yun-qi, CENG ; Qi-min, CHEN ; Xiao-ke, ZHU ; Jian-hui, XIA
Virologica Sinica 2001;16(2):109-113
Different-length fragments of the HIV trans-membrane antigen (gp120) gene were expressed in Escherichia coli, using T7 expression system. SDS-PAGE stained by Coomassic Brilliant Blue showed no expression of full-length gp120 and poor expression of half-length gp120 fragments from the N-terminal, but high expression of 1/3-lgenth gp120 gene fragments from the N-terminal (including V1/V2 epitopes), more than 18% of total bacterial protein. Western blot showed fairly good reactivity to serum from HIV-infected individual. On this basis, we expressed the corresponding tp 120 fragments of the HIV strains epidemic in China. This study laid a solid foundation for exploration of high expression of gp 120 in E.coli and development of HIV serological diagnostic system for Chinese people.
4.SUMO-2/3 can covalently bind to progesterone receptor B to regulate its transcriptional activity.
Bai-yu HAN ; Fa-ceng LI ; Long CHENG ; Xiao-jie XU ; Kai JIANG ; Jie FU ; Yong-jian HAN ; Zhao-hui LV ; Jing-tao DOU ; Hao ZHANG ; Qi-nong YE
Journal of Southern Medical University 2011;31(9):1493-1497
OBJECTIVETo investigate whether progesterone receptor B (PRB) can be sumoylated by SUMO-2/3 and the effect of sumoylation on PRB transcriptional activity.
METHODSSUMO-2/3 cDNA was amplified from MCF-7 cDNA and cloned into the eukaryotic expression vector pcDNA3-FLAG. The plasmid pXJ40-myc-PRB was cotransfected with pcDNA3FLAG-SUMO2, pcDNA3FLAG-SUMO3 or the mock control into 293T cells, and PRB sumoylation was detected by immunoprecipitation and Western blotting. The effect of PRB sumoylation on its transcriptional activity was determined using reporter luciferase assay.
RESULTSpcDNA3FLAG-SUMO2 and pcDNA3FLAG-SUMO3 vectors were successfully constructed. SUMO-2/3 could bind covalently to PRB and increase its transcriptional dependent on the presence of progesterone.
CONCLUSIONPRB can be sumoylated by SUMO-2/3 and its function is regulated by this modification.
Animals ; Cell Line ; Humans ; Plasmids ; genetics ; Receptors, Progesterone ; genetics ; metabolism ; Small Ubiquitin-Related Modifier Proteins ; genetics ; metabolism ; Transcription, Genetic ; Transfection ; Ubiquitination ; Ubiquitins ; genetics ; metabolism
5.Spatial Distribution of Parvalbumin-Positive Fibers in the Mouse Brain and Their Alterations in Mouse Models of Temporal Lobe Epilepsy and Parkinson's Disease.
Changgeng SONG ; Yan ZHAO ; Jiajia ZHANG ; Ziyi DONG ; Xin KANG ; Yuqi PAN ; Jinle DU ; Yiting GAO ; Haifeng ZHANG ; Ye XI ; Hui DING ; Fang KUANG ; Wenting WANG ; Ceng LUO ; Zhengping ZHANG ; Qinpeng ZHAO ; Jiazhou YANG ; Wen JIANG ; Shengxi WU ; Fang GAO
Neuroscience Bulletin 2023;39(11):1683-1702
Parvalbumin interneurons belong to the major types of GABAergic interneurons. Although the distribution and pathological alterations of parvalbumin interneuron somata have been widely studied, the distribution and vulnerability of the neurites and fibers extending from parvalbumin interneurons have not been detailly interrogated. Through the Cre recombinase-reporter system, we visualized parvalbumin-positive fibers and thoroughly investigated their spatial distribution in the mouse brain. We found that parvalbumin fibers are widely distributed in the brain with specific morphological characteristics in different regions, among which the cortex and thalamus exhibited the most intense parvalbumin signals. In regions such as the striatum and optic tract, even long-range thick parvalbumin projections were detected. Furthermore, in mouse models of temporal lobe epilepsy and Parkinson's disease, parvalbumin fibers suffered both massive and subtle morphological alterations. Our study provides an overview of parvalbumin fibers in the brain and emphasizes the potential pathological implications of parvalbumin fiber alterations.
Mice
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Animals
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Epilepsy, Temporal Lobe/pathology*
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Parvalbumins/metabolism*
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Parkinson Disease/pathology*
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Neurons/metabolism*
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Interneurons/physiology*
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Disease Models, Animal
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Brain/pathology*
6.Correction: Spatial Distribution of Parvalbumin-Positive Fibers in the Mouse Brain and Their Alterations in Mouse Models of Temporal Lobe Epilepsy and Parkinson's Disease.
Changgeng SONG ; Yan ZHAO ; Jiajia ZHANG ; Ziyi DONG ; Xin KANG ; Yuqi PAN ; Jinle DU ; Yiting GAO ; Haifeng ZHANG ; Ye XI ; Hui DING ; Fang KUANG ; Wenting WANG ; Ceng LUO ; Zhengping ZHANG ; Qinpeng ZHAO ; Jiazhou YANG ; Wen JIANG ; Shengxi WU ; Fang GAO
Neuroscience Bulletin 2023;39(11):1747-1748