The study on human embryonic stem cell regeneration is one of the current hot issues of life science.Dialectically analyzing its benefits and harms and the scientific application of the therapy of human diseases.It will surely initiate a new model of clinic treatment.

Objective: To construct the eukaryotic expression recombinant plasmid of cadF gene of Campylobacter jejuni Pen: 19 and to identify its express in COS-7 cells.Methods: Total DNA of Campylobacter jejuni Pen:2?Pen:8?Pen:19 and Pen: 21 were used as templates respectively and cadF gene DNA with KpnI and EcoRI sites were amplified by PCR reactions using same primer.Because of its closest association with Guillain-Barr? syndrome,the PCR product of Pen:19 was selected as target gene and inserted into the eukaryotic expression vector pcDNA3.1(+) and the recombinant plasmid pcDNA3.1(+)-cadF was constructed.The recombinant plasmid was identified by restriction enzyme digestion and PCR reaction and was confirmed by sequencing.The recombinant plasmid was then transfected into mammalian cell COS-7 for mRNA expression and the expression were investigated by RT-PCR reaction.Results: It was confirmed with restriction enzyme digestion and PCR reaction and sequencing that cadF gene was cloned into pcDNA3.1(+) successfully.The objective gene was amplified from COS-7 cell transfected with the recombinant plasmid pcDNA3.1(+)-cadF by RT-PCR.Conclusion: The eukaryotic expression recombinant plasmid pcDNA3.1(+)-cadF is constructed and expressed in COS-7 cell successfully.The results obtained lay the foundation for research on development of Campylobacter jejuni DNA vaccine.

Objective To evaluate the diagnostic value of serum angiopoietin-2 (Ang-2) level for gastric cancer.Methods Serum Ang-2 level waa measured by enzyme-linked immunosorbent assays (ELISA) in samples from 108 patients with gastric cancer,50 patients with chronic gastritis and 50 normal controls.Results The serum Ang-2 level in patients with gastric cancer [(1539.0 ± 449.3) ng/L] was significantly higher than that in patients with gastritis [(1044.6 ± 246.1) ng/L,P ＜ 0.01] and normal control [(1075.6 ± 228.2) ng/L,P ＜ 0.01)].There was no significant difference of serum Ang-2 level between patients with gastritis and normal control.Gastric cancer patients with lymph node metastasis had significantly higher serum Ang-2 level than those without metastasis (P ＜0.01).The area under ROC curve of serum Ang-2 level for diagnosis of gastric cancer was 0.819.Conclusion The serum Ang-2 level will be a useful indicator for diagnosis of gastric cancer.

At present,in the opinions of the domestic and foreign scholars,the only hope of long-term survival even cure of hilar cholangiocarcinoma was obtained by radical resection(ie R0 resection).The application of preoperative assessment with appropriate and comprehensive strategies can judge the respectability and Intraoperative safety accurately to the tumor,thereby it can improve the radical resection rate and safety of patients with hilar cholangiocarcinoma.In this paper,preoperative assessment strategies are reviewed.

BACKGROUND: Severe or prolonged stress is harmful to health and even induces many mental and physical disorders and then impairs cognitire functions of the organism.OBJECTIVE: To investigate the effects of stress on the changes in amino acid and chronic stress on long-term potentiation (LTP) of hippocampus of rats.DESIGN: Completely randomized controlled experiment.SETTING: Mental Health Center of Shantou University.MATERIALS: The experiment was completed in the Medical College of Shantou University in December 2000. Totally 16 adult male SD rats were divided randomly into control group and stress group with 8 in each group.METHODS: Mice in stress group were swum compulsively for 4 weeds.Chronic stress model was established, and isolated hippocampal slice(500 μm) combined with electrophysiological technique was used to observe the changes of LTP in hippocampal CA1 field. High-frequency stimulation (HFS) was applied to Schaffer collaterals in hippocampus CA3, then the changes of PS amplitude and fEPSPs slope in CA1 pyramidal neurons were observed. High performance liquid chromatography (HPLC) coupled with UV detection was used for the quantificative analysis of hippocampal amino-acid neurotransmitters.MAIN OUTCOME MEASURES: ① Population spike (PS) amplitude and field excitatory postsynaptic potentials (fEPSPs) slope were used to indicate the changes of LTP. ② Changes of amino acid of hippocampus.RESULTS: One rat in stress group died because of drowning in the second week of experiment was supplied, and others entered the final analysis. ① The increases of PS amplitude and fEPSPs slope after HFS in control group were significantly higher than those in stress group (P＜ 0.05). ② The levels of aspartate and glutamate in control group were significantly lower than those in stress group [(2.425±0.211) μmol/g,(6.016±0.677) μmol/g. P＜ 0.01; (4.746±0.609) μmol/g. (8.094±1.035) μmol/g.P ＜ 0.01]; but there was no significant difference of γ-aminobutyric acid between control and stress groups [(4.229±0.449) μmol/g, (4.249± 0.463) μmol/g, P ＞ 0.05].CONCLUSION: It is suggested that chronic stress can inhibit the development of LTP in hippocampal CA1 field and increase the levels of hippocampal aspartate and glutamate, but does not affect the level of γ-aminobutyric acid. The accumulation of hippocampal excitatory amino acids induced by chronic stress may be the neurobiochemical basis of impairments in learning and memory abilities.

AlM: To observe the clinical curative effect of calcium dobesilate capsule and yimaikang tablet for treating retinal vein occlusion ( RVO) .METHODS:A total of 120 patients (167 eyes) with RVO were divided into 2 groups at random. The control group of 60 cases ( 82 eyes ) were given calcium dobesilate capsule, and the treatment group of 60 cases ( 85 eyes ) received calcium dobesilate capsule and yimaikang tablet. Changes of visual acuity and clinical effects after 3 courses of treatment were compared and analyzed for all patients.RESULTS: Markedly effective 15 cases ( 23 eyes ) , effective 42 cases (57 eyes) and invalid 3 cases (5 eyes) in the 60 cases (85 eyes) of the treatment group, the total effective rate was 94. 1%. Eighty-two eyes of 60 cases in the control group, markedly effective in 8 cases ( 12 eyes), effective 38 cases (49 eyes), ineffective 14 cases (21 eyes), The total effective rate was 74. 4% in the control group. There were significant differences between the 2 groups of curative effect, and low incidence rate of adverse reaction. CONCLUSlON: Calcium dobesilate capsules and yimaikang tablet is effective and safe in the treatment of RVO.

Objective To study the outcome of pregnancy with lupus nephritis. Methods The clinical data of 9 pregnancies with lupus nephritis were retrospectively malyzed. Results Hypertension,proteinuria,injured renal function and abnormal serological marker were complicated with nine patients with lupus nephritis in pregnancy. The outcome of pregnancy was as following:abortion 2 cases,stillbirth 1 case,premature delivery 4 cases,term delivery 2 cases. All neonate survived without malformation. There was no postpartum hemorrhage or death. Patients with active SLE had very high fetal mortality( 100% ). And SLE got worsen in 50% of them. However,fetal mortality rate in pa-tients with controlled or quiescent SLE was 11.1% ,which was much lower than it in other patients(P<0.05). Con-clusion Lupus nephritis should be controlled before pregnancy. Pregnancy outcome could be improved by careful multidisciplinary monitoring and proper usage of adrenal cortical hormone.

A totel of 120 patients with CT or MRI proved as acute cerebral infarction group of anterior circulation and 90 non-infarction patients as the control group were studied. The diameter,inter median thickness( IMT) ,peak systolic velocity ( PSV ) , end diastolic velocity ( EDV ) , early shrinkage acceleration time ( AT ) , resistance index ( RI) ,and pulsatility index( PI) of carotid artery,and whether there was arteriosclerotic plaque or stenosis,and the properties of plaque were examined with color Doppler ultrasound. In acute cerebral infarction group of anterior cir-culation,the IMT was thicker that in the control group(P<0. 01). The incidence of the film thickness,moderate-se-vere stenosis and instable carotid artery plaque in infaiction group of anterior circulation were obviousely higher than those in the control group(P<0. 01). Arterial complete or incomplete occlusion was observed in severe cases. The blood flow parameters showed that the PSV and EDV of common carotid and internal carotid in acute cerebral in-farction group of anterior circulation were lowerer than those in the control group. RI and PI of internal carotid in a-cute cerebral infarction group of anterior circulation were higher than those in the control group(P<0. 05),while AT was lower than those in the control group.

Objective To explore left ventricular longitudinal axis systolic asynchrony by real-time triplane quanti-tativetissue velocity imaging(RT-3PE QTVI) and two-dimensional speckle tracking imaging(STI) in patients with dilated cardiomyopathy. Methods Thirty normal adults were included as the control group and thirty adults with DCM. The images from enough frame rates RT-3PE QTVI and STI were obtained from series long-axis views. Simp-son method was used to measure LVEF. Q-lab software was used to measure peak systolic tissue velocity(Vs) and peak systolic longitudinal strain (Ls),its time to peak point from each segment(Ts,Tls) and the maximal temporal difference of Ts,Tls(Ts-diff,Tls-diff),and the standard deviation of. Ts,Tls(Ts-SD,Tls-SD),which were served as systolic asynchrony indexes in assessing LV longitudinal axis systolic asynchrony. Results ① Compared the con-trol group and DCM group,there were significant differences in LVEDd,LVESd,LVEDL,LVESL,LVEF,FS (P<0.01). ② In comparison with the control group,the Ls and Vs of each segment were decreased in the DCM group (P<0.01). Compared with the control group,the parameters of Tls and Ts in DCM group had obvious ahead or de-layed(P<0.05). ③ Compared with the control group,the parameters of Ts-SD, Tls-SD, Ts-diff, Tls-diff,were significantly different in DCM group (P<0.01). Ts-SD,Tls-SD,Ts-diff,Tls-diff corrrlated negatively to LVEF(r=-0.67,-0.72,-0.75, -0.77,P<0.01). Conclusion The prevalence of left ventricular longitndinal axis systolic asynchrony exits in patients with DCM. There is a better technique to evaluate LV longitudinal axis systolic asynchrony by RT-3PE QTVI and STI than only one.

Hepatitis A is one of the global public health problems,and China has high prevalence. In order to decrease the incidence of hepatitis A,China has integrated Hepatitis A vaccine into Expanded Program on Immunization (EPI) in 2007. The paper will provide some ideas on the immunogenicity and impact factors of the different types of hepatitis A vaccines that are used in China.