1.The inflatable penile prosthesis made in China for treatment of erectile dysfunction.
Ji-hong LIU ; Yan ZHANG ; Zheng LI ; Yi Dong LIU ; Bing CHEN
Chinese Journal of Medical Instrumentation 2005;29(4):267-269
Erectile dysfunction (ED) is a common male sexual dysfunction. Some ED patients who do not respond to any nonsurgical therapy will eventually be candidate for penile prostheses. This paper introduces the characteristics and application of inflatable penile prosthesis made in China.
Erectile Dysfunction
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therapy
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Humans
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Male
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Penile Prosthesis
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Prosthesis Design
2.Pathogenicity evaluation and splicing correction of COL4A5 splice mutation in X-linked Alport syndrome
Tianrong ZHOU ; Huhan ZHANG ; Chunyan WU ; Qi LI ; Sheng HUANG ; Yong DUAN
Chinese Journal of Nephrology 2021;37(11):872-880
Objective:To analyze the splicing mutation site of COL4A5 gene in a family with X-linked dominant Alport syndrome and explore the possibility of exon specific U1 small nuclear RNA (snRNA) gene therapy. Methods:The clinical data of the proband and family members of Alport syndrome were collected, and the gene mutations in the whole exon of a series of nephropathy genes in the proband were detected by high-throughput sequencing. The splice site changes and pathogenicity caused by COL4A5 c.546+5G>A mutation were analyzed by online software. Minigene experiment was used to verify and analyze the effect of COL4A5 gene mutation site c.546+5G>A in the proband of Alport syndrome family, and transient transfection and introduction of modified U1 snRNA to correct splicing mutation. Results:The results of gene sequencing showed that there was a hemizygous variation of COL4A5 gene in the proband and his half brother, and the variation site was c.546+5G>A. The results of online software for analyzing the pathogenicity of splice variation showed that the original donor splicing site could not be detected after mutation, suggesting that there was a great possibility of affecting splicing. The abnormal splicing mode of COL4A5 gene with c.546+5G>A mutation—deletion of exon 9 was verified by hybridized small gene detection. The abnormal splicing mutation could be partially corrected by the modified U1 snRNA. The correction ratios of ExSpeU1 (MT), ExSpeU1(E9+1), ExSpeU1(E9+9) and ExSpeU1(E9+11) to exon 9 deletion caused by c.546+5G>A were 0, 43.81%, 52.09% and 48.12%, respectively. Conclusions:The pathogenicity of the new splicing mutation of COL4A5 is verified, and the modified U1 snRNA can partially correct the abnormal splicing.
3. Effect of short-term mindfulness intervention on anxiety and perceptual stress among senior high school students
Chunyang ZHANG ; Shixun JIANG ; Kangjie ZUO ; Wei XU
Chinese Journal of Behavioral Medicine and Brain Science 2019;28(9):797-801
Objective:
To explore the internal mechanism of short-term mindfulness intervention in reducing individual anxiety and stress perception.
Methods:
Totally 134 subjects were recruited and randomized into attention group (