Objective To study the changes of blood gastrointestinal hormones concentration and effect of early feeding in premature infants on it. Methods Seventy-two premature infants were divided into 4 groups: self-sucking (group Ⅰ),tube feeding (group Ⅱ),minimal enteral nutrition (MEN, group Ⅲ), non-nutritive sucking (NNS, group Ⅳ). The plasma motilin and serum gastrin were measured within 12 hour before enteral feeding, on day 3 and day 7 of life by radioimmunoassay. 16 full-term neonates were studied as control. Results (1)The serum concentration of gastrin in premature infants before enteral feeding, on day 3 and day 7 were (61?24)ng/L,(93?37)ng/L,(126?45) ng/L respectively, which were significantly lower than that of control(126?30) ng/L,(135?34)ng/L,and (178?46)ng/L,(P

OBJECTIVE: To study the correlation between quantity of the connexin43 express and ischemia time in rabbit. METHODS: Left coronary arteries were ligated to create early myocardial ischemia during distinct time, HBFP staining was used in the ischemia spot and SP method was used to show the expression of connexin43 and the ischemia are were measured by the computer image analysis. RESULTS: Negative correlation was observed between the connexin43 quantity and the ischemia time. The significant was difference. CONCLUSION With ischemia time raised, expression of connexin43 was descented.
Animals ; Connexin 43/metabolism* ; Female ; Immunohistochemistry ; Male ; Myocardial Ischemia/pathology* ; Myocardium/pathology* ; Rabbits ; Staining and Labeling/methods* ; Time Factors

We examined the biocompatibility and the safety of a-calcium sulfate hemihydrate (CSH)/multi-walled carbon nanotube (MWCNT) composites for bone reconstruction application. The biocompatibility of the CSH/MWCNT composites was evaluated by the measures which taking L929 fibroblast cells cultured in the extracted liquid of the composite soaking solution and putting bone marrow stromal cells planted on the composite pellets in vitro, respectively. The cell proliferation was evaluated by MTT test and further observed using an inverted optical microscope and a scanning electric microscope. The toxicity of the composites was evaluated by acute and subacute systemic toxicity test. Long-term muscle and bone implantation in vivo tests were also conducted. L929 fibroblast cells grew well in the extracted liquid, as well as bone marrow stromal cells that could adhere on the surface of sample pellets and proliferated rapidly. MTT test showed that there were no significant differences between the experimental and control groups (P > 0.05). In vivo test manifested that the composites were no toxicity, no irritation to skin and good for bone defect reconstruction. It was proved that a-calcium sulfate hemihydrate (CSH)/multi-walled carbon nanotube (MWCNT) composites exhibited excellent biocompatibility for the potential application in bone tissue engineering.
Animals ; Biocompatible Materials ; chemistry ; Bone Marrow Cells ; cytology ; Bone Substitutes ; chemical synthesis ; chemistry ; Calcium Sulfate ; chemistry ; Cell Line ; Cell Proliferation ; Fibroblasts ; cytology ; Materials Testing ; Nanotubes, Carbon ; chemistry ; Rabbits ; Stromal Cells ; cytology ; Tissue Engineering ; methods ; Toxicity Tests