Objective To study the effect of prodnisolone on lipopolysaccharide(LPS)-induced RANTES by microglia.Methods The microglia were incubated in MEM culture medium,and different concentrations of LPS 0,100,500,1000?g/L) or LPS(100?g/L) and prodnisolone(10?mol/L) were added into the culture medium.12h later,the amount of RANTES was measured by ELISA.Results Microglia markedly produced RANTES in response to LPS.Prodnisolone significantly reduced the production of RANTES in microglia.Conclusion Prodnisolone has an inhibitive effect on the production of chemokines such as RANTES.

Objective To observe the effects of wogonin on lipopolysaccharide(LPS-induced) proinflammatory cytokine production in activated microglia.Methods By using LPS-induced microglia activation model,nitric oxide(NO),tumor necrosis factor-alpha(TNF-?) and interleukin-6(IL-6) secreted by extracellular fluid were measured by ELISA.Results Microglia markedly produced NO,TNF-? and IL-6 in response to LPS.Wogonin,administered at 20-30 ?mol/L,significantly reduced the production of NO,TNF-? and IL-6 in microglia.Conclusion Wogonin has an inhibitory effect on the production of proinflammatory cytokines.