Objective: To investigate the anti-inflammatory effect and the possible mechanism of water and ethanol extracts of propolis. Method: Forty male Wistar rats were divided into 5 groups: normal group, model group, medicine groups, two groups treated with water and ethanol extracts of propolis. The acute pleurisy model was established by injecting carrageenan. The effects of propolis on acute pleurisy was studied by counting leukocytes, measuring the content of MDA, lysozyme and activity of SOD in serum and the content of NO, protein and PGE2 in pleural effusion. Results: The propolis solutions extracted by water and ethanol presented obvious effect on inflammation. It could antagonize the purulent pleurisy, reduce the number of leukocytes and the content of MDA, lysozyme and activity of SOD in serum and the contents of NO, protein and PGE2 and decrease the inflammation. Conclusion: Propolis displays anti-inflammatory effects by decreasing the action of NO and PGE2 and preventing the activation of protein kinase.

AIM: To study the effect of propolis on the expression of CD54 and activation of NF-?B p65 in lung tissue of acute lung injury (ALI) rats. METHODS: 40 male Wistar rats were divided into 5 groups: normal control, model control, dectancyl group, water soluble derivative of propolis (WSP) group and ethanol extracted propolis (EEP) group. ALI animal model was performed by oleic acid and LPS twice attack. The pathologic slice was observed with light microscope and the NF-?B p65 activity and CD54 expression were tested by immunohistochemistry (SABC and SP). RESULTS: Both EEP and WSP antagonized the lung edema, decreased the inflammation and inhibited the expression of CD54 and activation of NF-?B p65. CONCLUSION: The increase in the expression of CD54 and the activation of NF-?B p65 in the lung tissues of ALI were involved in the formation of ALI. Propolis ameliorated the lung damage, which maybe related to the inhibition of CD54 expression and NF-?B p65 activation.

Objective To establish a detection method of Salmonella in laboratory animals based on a high-throughput sequencing technology, and to apply it in detection of Salmonella in laboratory animals.Methods DNA samples were extracted from mouse feces.Universal primers for 16S rDNA, 23S rDNA, 16S-23S rDNA, 23S-5S rDNA region, gyrB preferred area were designed, respectively.Each primer was tested and analyzed to determine the best amplification conditions and build a database.Forty-two samples of Salmonella were assayed by Illumina high-throughput sequencing technology and evaluated the specificity and stability of this method.Results The species preferred region of Salmonella was gyrB gene region.The primers for gyrB gene were FP5 ’-AACCACCGCAATCAGACCTT3‘ and FP5 ’-AGCCACGAAACCTTCACYA-3’.The primers were optimized and determined, through a high-throughput sequencing, and the sequence analysis detected very small amount of Salmonella in the 42 samples, indicating that this detection method is stable, highly sensitive, and the limit of detection reached to 0-102 CFU.Conclusions We have established a complete detection system for detection of Salmonella in laboratory animals based on a high-throughput sequencing technology, This system can detect trace amounts of Salmonella in laboratory animals, and this detection method is stable and highly sensitive, which can be also used in detection of other kinds of pathogenic microorganism in laboratory animals.

An ECG waves separation technique based on math ematical morphology is suggested and discussed in this paper. Without detection of QRS complexes, the QRS complexes can be eliminated by series morphological o perations. Then the start points and end points of P-wave and T-wave can be de cided and the waves of ECG are separated qualitatively and quantitatively. The r esults of qualitative separations are rather satisfied and the SDs of quantitative separation results are not very large. Also, ECG signals are filtered and bas eline is normalized by morphological method.

In the paper, iridology and computer-aided iridiagnosis technologies are briefly introduced and the extraction method of the collarette contour is then investigated. The iris map can be overlapped on the original iris image based on collarette contour extraction. The research on collarette contour extraction and iris map overlap is of great importance to computer-aided iridiagnosis technologies.
Diagnosis, Computer-Assisted ; methods ; Humans ; Image Processing, Computer-Assisted ; methods ; Iris Diseases ; diagnosis ; Software

Objective To understand the molluscicidal effects of Eomecon Chionantha Alkaloids (ECA) on Oncomelania hupensis.Methods By using immersion methods for different durations of time the molluscicidal effects of ECA and the rate of snails climbing-upward were observed. Results While the solution concentrations and temperatures of ECA were at 1.25 mg/L,30℃;2.5 mg/L,25℃;5 mg/L and 10 mg/L,25℃ respectively,and the snails were immersed for 72 hours,the mortality of Oncomelania snails were 100%. The hatching rates of Oncomelania eggs after immersed for 72 h in the solution of 5 mg/L were 0～10%. ECA showed an inhibiting effect on the snails' climbing-upward in the concentrations of 5 mg/L. Conclusion ECA has molluscicidal effect on Oncomelania and is one of the potential plant molluscicides.

Background: Helicobacter pylori (Hp) infection is closely related to a variety of extraintestinal diseases such as coronary heart disease and diabetes, and the mechanism is not yet clear. Low serum bilirubin level is a risk factor of these extraintestinal diseases and may be involved in the pathogenic mechanism of Hp infection. Aims: To investigate the correlation of Hp infection with serum bilirubin. Methods: A total of 10 843 individuals taken routine physical examinations from June 2016 to June 2017 at Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology were enrolled. Hp infection was detected. Hp infection rate was compared between groups with different gender, age and BMI. Serum total bilirubin (TBil), direct bilirubin (DBil), indirect bilirubin (IBil) levels between Hp-positive group and Hp-negative group were compared. Correlation of Hp infection with serum bilirubin was analyzed. Results: Hp infection rate was 35.0%. No significant difference in Hp infection rate was found between groups with different gender. However, Hp infection rate was statistically different between groups with different age or BMI (P<0.001). Compared with Hp-negative group, serum TBil, DBil, IBil levels were significantly decreased in Hp-positive group (P<0.001). Multiple linear regression analysis showed that Hp infection was negatively correlated with serum TBil, DBil, IBil levels [regression coefficient was -0.805 (95% CI: -1.256-0.353), -0.134 (95% CI: -0.243-0.026), -0.667 (95% CI: -1.047-0.287), respectively; P<0.001, P=0.015, P=0.001]. Conclusions: Hp infection is negatively correlated with serum TBil, DBil and IBil levels. Hp infection may be involved in the pathogenesis of extraintestinal diseases such as coronary heart disease and diabetes via decreasing serum TBil, DBil and IBil levels.

Objective: To determine the effect of neoadjuvant chemotherapy on the overall survival (OS) in patients with International Federation of Gynecology and Obstetrics (IFGO) stage I B cervical cancer. Methods: The randomized controlled trials and retrospective casecontrol studies on neoadjuvant chemotherapy plus radical surgery vs initial radical surgery in patients with IFGO stage I B cervical cancer were searched in PubMed, EMBASE and CENTRAL (Cochrane Central Register of Controlled Trials) databases, and the subgroup analysis was performed according to the type of the study. The hazard ratio (HR) and 95% confidence interval (CI) were used to describe the effect of neoadjuvant chemotherapy on OS of FIGO stage I B cervical cancer patients. The quality of randomized controlled trials was evaluated by Cochrane Collaboration's tool for assessing the risk of bias. The quality of retrospective studies was evaluated according to the Newcastle-Ottawa Scale (NOS) scoring system. The publication bias was tested. Results: Three randomized controlled trials and three retrospective observational studies involving 1 080 stage I B patients with cervical cancer were included in this Meta-analysis. The combined HR estimate of OS for stage I B patients with cervical cancer receiving neoadjuvant chemotherapy plus surgery was 0.71 (95% CI: 0.55-0.93) using fixed effect model. In Meta-analysis of randomized controlled trials, the combined HR estimate of OS for stage I B patients with cervical cancer receiving neoadjuvant chemotherapy plus surgery was 0.77 (95% CI: 0.58-1.03) using fixed effect model. In Meta-analysis of retrospective observational studies, the combined HR estimate of OS for stage I B patients with cervical cancer receiving neoadjuvant chemotherapy plus surgery was 0.45 (95% CI: 0.23-0.88). The funnel map showed no publication bias. Conclusion: Neoadjuvant chemotherapy can significantly improve the OS of stage I B cervical cancer patients. Additional randomized controlled studies with large-scale sample are needed to verify this survival beneft.

OBJECTIVE:To establish the method for the content determination of rapamycin (RAPA) in human monocyte THP-1 derived foam cells,and to study the effects of RAPA targeting preparation(RAPA-NP-Apt)targeting at foam cells. METH-ODS:Foam cells model were established through THP-1 cells were induced by oxidized low density lipoprotein. Foam cells were incubated with 200 ng/mL RAPA or 200,400,800 ng/mL RAPA-NP-Apt for 60 min. The content of RAPA was determined by HPLC. The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-water(90:10,V/V) at flow rate of 1.0 mL/min. The column temperature was set at 40 ℃,and the detection wavelength was 278 nm. The sample size was 20 μL. RESULTS:The concentration of RAPA ranged 50-6400 ng/mL (r=0.99996) with average recovery of 98.72%(RSD=0.62%,n=3). RSDs of inter-day and intra-day were not more than 6.15%(n=6),RSD of stability was lower than 2%(n=6),and RSD of repeatability was 1.64%(n=6). After foam cells were incubated with RAPA or low-concentration,medi-um-concentration and high-concentration of RAPA-NP-Apt,the contents of RAPA were 12,43,98,140 ng/106 cells. CONCLU-SIONS:The method is simple,stable and reproducible. It can be used for content determination of RAPA in foam cells. RA-PA-NP-Apt can improve the effects of RAPA targeting at foam cells.

The accuracy and diagnostic values of transcranial Doppler (TCD) in transient ischemic attack (TIA) caused by the stenosis or occlusion of intracranial or extracranial blood vessels were investigated. Of the 50 TIA patients receiving routine TCD examinations, 39 cases (77 %) were diagnosed as having unilateral or bilateral stenosis or occlusion of MCA, ACA, siphon segment of internal carotid artery, which was furthermore confirmed by digital subtraction angiography (DSA) or MRA, 11 (22 %) cases were normal. An analysis on the TCD findings of the flow patterns and hemodynamic changes of the stenotic or occluded blood vessels was made in comparison with that obtained by angiography. It was showed that angiography demonstrated 17 unilateral MCA stenosis, 47 bilateral multi-stenosis, 1 occlusion of the siphon segment of the internal carotid artery, while TCD revealed 17 unilateral stenosis, 64 bilateral stenosis, 1 occlusion respectively, with an accordance rate of 78.7 %. It was concluded that the hemodynamic changes secondary to the stenosis of the basalcranial arteries, especially the moderate to severe stenosis or occlusion, might be an important risk factor for TIA. TCD examination achieved significant clinic values in the diagnosing of TIA.