OBJECTIVETo study the molecular epidemiological characteristics of Salmonella in animal source foods in Hunan.

METHODSThe fair trade markets and supermarkets of ten cities were chosen to sample animal source foods for isolating Salmonella in Hunan province in 2010. A total of 692 samples were collected by aseptic sampling, included 159 livestock meats, 152 poultry meats, and 381 aquatic products.Salmonella strains isolated were subjected to stereotyping, antimicrobial susceptibility testing and pulsed field gel electrophoresis (PFGE).

RESULTSSalmonella was detected in 93 of 692 animal food samples with the detection rate of 13.4%. The detection rates of Salmonella in poultry meats, livestock meats and aquatic products were 23.0% (35/152), 22.6% (36/159) and 5.8% (22/381) respectively. Therefore, the detection rate in aquatic products was lower than that of poultry meats and livestock meats (χ(2) = 33.86, P < 0.05; χ(2) = 33.29, P < 0.05, respectively). The serotypes of isolates showed diversity, and Salmonella Derby (33/94, 35.1%) was the predominant serotypes.79.8% (75/94) strains showed resistant to more than one antibiotic used in the test, 31.9% (30/94) strains showed resistant to more than 5 antibiotics. A significant difference was observed for multidrug resistance between Salmonella isolated from poultry (47.2%, 17/36) and livestock meats (22.2%, 8/36) (χ(2) = 4.96, P < 0.05). And the highest resistant rate was found in tetracycline, as high as 62.8% (59/94). All the strains were divided into 69 PFGE subtypes.Furthermore the dominating subtypes were type 7 (6 strains), type 15 (4 strains), type 22 (6 strains).

CONCLUSIONInspection results showed that Salmonella contamination in animal source foods were serious in Hunan province, and the isolates expressed high level resistance to the antibiotics.Furthermore the PFGE results indicated that there were epidemic strains of Salmonella in Hunan.

Animals ; Anti-Bacterial Agents ; China ; epidemiology ; Drug Resistance, Multiple, Bacterial ; Electrophoresis, Gel, Pulsed-Field ; Food Microbiology ; Meat ; microbiology ; Microbial Sensitivity Tests ; Poultry ; microbiology ; Salmonella ; classification ; Salmonella enterica ; Seafood ; microbiology ; Tetracycline Resistance

Objective To explore the effects of WeChat-based self-management education on patients with depression. Methods Totally 108 patients with depression who were discharged from a municipal mental health center between January and December 2016 received self-management education with the WeChat platform for six months. The frequency of reading push contents on WeChat, Insight and Treatment Attitude Questionnaire (ITAQ), Medication Adherence Questionnaire and Beliefs About Medical Questionnaire (BMQ) were used to assess the patients one month and six months after the management. The patients' insight and treatment attitude as well as medication adherence before and after the introduction of WeChat-based self-management education was compared. Results After six months of WeChat-based self-management education, the patients with depression showed better frequency of reading disease publicity materials, personized guidance, WeChat games and watching video and pictures in push contents of WeChat than that after one month of self-management (P<0.05); the patients showed higher insight and treatment attitude scores, lower medication adherence scores and better scores in the dimensions of beliefs about medical than that after one month of self-management (P<0.01). Conclusions WeChat-based self-management education can effectively improve the adherence, perception to disease and rehabilitation of those depression patients discharged from hospital.

Objective: To analyze the molecular characteristics of Listeria monocytogenes strains from ready-to eat food in China. Methods: A total of 239 Listeria monocytogenes strains isolated from ready-to-eat food in 2017, all strains underwent whole-genome sequencing (WGS) , and comparisons uncovered population structure derived from lineages, clonal complex, serogroups, antimicrobial susceptibility and virulence, which were inferred in silico from the WGS data. Core genome multilocus sequence typing was used to subtype isolates. Results: All strains were categorized into three different lineages, lineage Ⅱ was the predominant types in food, and IIa was the main serogroups. CC8, CC101 and CC87 were the first three prevalent CCs among 23 detected CCs, accounting for 49.4%. Only 4.6% (11 isolates) of tested strains harbored antibiotic resistance genes, which were mostly trimethoprim genes (7 isolates, 2.9%). All strains were positive for LIPI-1, and only a part of strains harbored LIPI-3 and LIPI-4, accounting for 13.8% (33 isolates) and 14.2% (34 isolates), respectively. ST619 carried both LIPI-3 and LIPI-4. 51.5% (123 isolates) of strains carried SSI-1, and all CC121 strains harbored SSI-2. Different lineages, serogroups and CCs can be separated obviously through cgMLST analysis, and 24 sublineages were highly concordant with CCs. Conclusion Ⅱa was the main serogroups in ready-to-eat food isolates in China; CC8, CC101 and CC87 were the prevalent CCs, and CC87 isolates was hypervirulent isolates, cgMLST method can be adopted for prospective foodborne disease surveillance and outbreaks detection.