Objective:To explore the effects of probiotics on non-motor symptoms in patients with Parkinson disease through Meta-analysis, so as to provide objective evidence for the clinical practice of probiotics on non-motor symptoms in patients with Parkinson disease.Methods:Randomized controlled trials on probiotics for non-motor symptoms of Parkinson disease were searched in Cochrane Library, PubMed, Embase, Web of Science, China National Knowledge Infrastructure, VIP, Wanfang Data and SinoMed. The search time limit was from the establishment of the database to September 1, 2022. Two researchers independently conducted article screening, data extraction, and quality evaluation, and used RevMan 5.4.1 for Meta-analysis.Results:A total of 543 patients were included in 8 articles. The results of Meta-analysis showed that the clinical efficacy of probiotics on constipation in patients with Parkinson disease [ OR=7.25, 95% CI (2.00, 26.23) , P=0.003], constipation symptoms [ SMD=1.21, 95% CI (0.38, 2.04) , P=0.004], constipation-associated quality of life [ SMD=0.93, 95% CI (0.60, 1.27) , P<0.001], average number of weekly spontaneous defecation [ SMD=0.80, 95% CI (0.42, 1.17) , P<0.001], anxiety [ SMD=0.54, 95% CI (0.28, 0.80) , P<0.001], depression [ SMD=0.73, 95% CI (0.48, 0.97) , P<0.001] and sleep quality [ SMD=0.53, 95% CI (0.16, 0.90) , P=0.005] were better than those in the control group, with statistically significant differences. Conclusions:Probiotics can effectively improve constipation, anxiety, depression, and sleep disorders in non-motor symptoms of Parkinson disease patients.