1.Music Therapy for Verbal Development of Autistic Child:A Case Report
Chinese Journal of Rehabilitation Theory and Practice 2016;22(3):261-264
Objective To discuss the intervention of music therapy for autistic child in verbal development. Methods An autistic child accepted receptive and recreative music therapy, his development of verbal and relative abilities were observed. Results The verbal and rela-tive abilities significantly improved after treatment. Conclusion Music therapy is very effective on language development in the autistic child, as well as the development of moods, sensory perception, communication, behavior and cognition.
2.Effects of geldanamycin on expression of HSP,mutant p53 and CDK4 in human breast cancer cells
Journal of Third Military Medical University 2003;0(13):-
Objective To investigate the effect of geldanamycin(GA) on the expression of HSP(HSP90,HSP70,HSP27) and oncoprotein(mutant p53,CDK4) in human breast cancer cell line MDA-MB-435s.Methods Proliferation of MDA-MB-435s cells was measured with MTT assay.The mRNA expression levels of HSP90,HSP70 and HSP27 were detected by RT-PCR.The protein expression levels of HSP90,HSP70,HSP27,mutant p53 and CDK4 were detected by Western blot.Results GA inhibited the proliferation of MDA-MB-435s cells in a time-dose dependent manner.The expression level of mRNA and protein of HSP90,HSP70 and HSP27 were increased,and the increasing of HSP70 was the most significant,while the protein expression level of mutant p53 and CDK4 in MDA-MB-435s cells were reduced obviously after 48-hour treatment with 400 nmol/L GA as compared with that of control cells.Conclusion GA inhibits the proliferation of MDA-MB-435s cells by down-regulating the level of mutant p53 and CDK4 protein,and GA involves in the protection of cell stress through increasing the expression of HSP90,HSP70 and HSP27.
3.Clinical effect of cemented long stem prosthesis and memory alloy embracing fixator in the treatment of type B2 periprosthetic fractures
Clinical Medicine of China 2017;33(3):259-262
Objective To study the clinical effect of cemented long stem prosthesis and memory alloy embracing fixator in the treatment of type B2 periprosthetic fractures.Methods Fom April 2014 to April 2016,102 cases of peripheral B2 periprosthetic fractures patients were selected and randomly divided into bone cement long stem prosthesis revision surgery(group A) and revision of cemented long stem prosthesis combined with memory alloy embracing fixator(group B),each group with 51 cases.Clinical efficacy was evaluated according to Beals and Tower criteria,used Harris score to evaluate the hip the joint function,used the Visual Analogue Scale(VAS) score to evaluate the pain,after follow-up of 40 months,the clinical curative effect of 2 groups of patients,fracture healing time,complications and Harris score and VAS score were observed.Results The excellent rate of B group was significantly higher than that of A group,with statistical significance(90.2% vs.72.5%,χ2=5.239,P<0.05).Fracture healing time of group B was shorter than that of group A,with statistically significant ((10.2±2.5) weeks vs.(21.3±8.9) weeks,t=8.575,P<0.05).Harris the score of group B was higher than the group A,with statistically significant((89.9±13.5) points vs.(71.2±18.9) points,t=5.750,P<0.05).VAS score of group B was significantly lower than the group A,with statistically significant((1.23±0.25) points vs.(5.98±1.08) points,t=30.600,P<0.05).There were no complications such as infection,nonunion,loss of reduction and loosening of internal fixation in the 2 groups.Conclusion Revision of cemented long stem prosthesis combined with memory alloy embracing fixator is an ideal methods for the treatment of type B2 periprosthetic femoral fractures,which provides a good initial stability for fracture healing.
4.Role of extracellular signal-regulated protein kinase in estrogen-induced proliferation of breast cancer cell line MCF-7
Suhua LI ; Huayu DENG ; Li CHEN
Academic Journal of Second Military Medical University 1981;0(04):-
Objective:To investigate the role of extracellular signal-regulated protein kinase(ERK) in estrogen-induced proliferation and cell cycle transformation of breast cancer cell line MCF-7 and the related mechanisms.Methods: Estrogen receptor-positive breast cancer cell line MCF-7 was used in our study.The effects of 17?-E2 on the proliferation of MCF-7 cells was investigated by MTT assay to determine the optimal concentration of 17?-E2 for the following experiment.The effect of PD98059 on 17?-E2-induced proliferation of MCF-7 cells was measured by MTT assay to determine the intermediate concentration of PD98059.The cell cycle was analyzed by flow cytometry and telomerase activity was determined by Telomerase repeat amplification protocol PCR(TRAP-PCR) silver staining.The expression of wild-type p53 and phosphorylated ERK1/2 protein was determined by Western blotting and the expression of wild-type p53 mRNA was detected by RT-PCR.Results: ERK phosphorylation inhibitor PD98059 inhibited the proliferation of MCF-7 cells treated with 17?-E2 in a time-and dose-dependent manner(P
5.Effect of EGCG on invasion of breast cancer cells and its possible mechanism
Huayu DENG ; Li LU ; Weike FAN
Chinese Journal of Pathophysiology 2000;0(11):-
AIM: To study the effects of (-)-epigallocatechin-3-gallate (EGCG), a tea extract, on the invasion and metastasis of breast cancer cell line MDA-MB-231 and the possible mechanisms in vitro. METHODS: The expression of MUC1 in breast cancer cells treated with or without EGCG was detected by immunohistochemistry. The effect of EGCG on invasion of MDA-MB-231 cells was evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane (Matrigel). Gelatin zymography was performed to detect the secretion of collagenase-Ⅳ. RESULTS: EGCG reduced the expression of MUC1, significantly suppressed the invasion of tumor cells to basement membrane and reduced the secretion of collagenase-Ⅳ. CONCLUSION: In vitro, EGCG may suppress invasion, metastasis, and collagenase-Ⅳ secretion in MDA-MB-231 cells by inhibiting the production of MUC1.
6.Advance in Diagnosis for Carpal Tunnel Syndrome (review)
Yang LI ; Xiangyang ZHU ; Huayu HUANG
Chinese Journal of Rehabilitation Theory and Practice 2013;19(3):246-249
Carpal tunnel syndrome (CTS) is a common peripheral neuropathy, which include a series of symptoms results from median nerve injury. Electrical physical examination is the gold standard for diagnosis of CTS. More new technology, such as high frequency ultrasound and magnetic resonance imaging, provides more information for the diagnosis of CTS.
7.Ultrasound-guided parathyroid homogenate injection in anterior fascia of the flexor carpus vs. intramuscular transplantation for secondary hyperparathyroidism
Hao LI ; Huayu LI ; Peng LI ; Feng SHEN ; Dazheng FANG
Chinese Journal of General Surgery 2021;36(2):127-131
Objectives:To evaluate forearm intramuscular autograft Tx vs. forearm subcutaneous ultrasound-guided homogenate injection transplantation. Methods:One hundred-ten end-stage chronic renal failure patients who after total parathyroidectomy and autologous forearm Tx from Mar 2011 to Mar 2020 were enrolled for retrospective analysis.The control group received autologous parathyroid forearm intramuscular autograft Tx, and the observation group had the anterior fascia of the flexor carpus ultrasound-guided homogenate injection Tx. We evaluated the effects of transplantation between the two groups.Results:There were no significant differences in the levels of iPTH, blood calcium, blood phosphorus, and calcium-phosphorus product between the two groups of patients 30 minutes , 1 month , and 3 months after surgery.The two-dimensional ultrasound simulated ellipse dispersion volume in the observation group is much smaller than that in the control group [(2.51±0.41) cm 3vs. (1.91±0.55) cm 3, t=6.519, <0.001] The linear regression equation of the actual volume of the three-dimensional ultrasound in the observation group and the iPTH value in the observation group after 3 months is Y1=47.728+ 122.337X, r1=0.892, P<0.05. The linear regression equation of the two-dimensional ultrasound simulated ellipse diffusion volume in the observation group and the iPTH value in the observation group at 3 months after operation was Y2=121.408+ 81.736X, r2=0.883, P<0.05. Conclusion:Compared with intramuscular Tx, the recovery levels of iPTH, calcium, phosphorus and calcium-phosphorus product after the anterior fascia of the flexor carpus ultrasound-guided homogenate parathyroid glands injection Tx are similar, but its operation is more simple and diffusion volume is smaller.
8.Evaluation on method performance decision chart of automatic blood coagulation analyzer
Huayu CHEN ; Li ZHANG ; Nan LI ; Xu LIU
International Journal of Laboratory Medicine 2014;(15):2071-2072
Objective To adopt the method performance decision chart to evaluate the performance of the Stago R Evolution au-tomatic blood coagulation analyzer .Methods The method performance decision chart was drawn by the results of external quality assessment and internal quality control according to the requirement of the total error in the clinical laboratory improvement amend-ment (CLIA′88) .The 6 indexes of 3 items including prothrombin time(PT) ,activated partial thromboplastin time(APTT) and fi-brin (Fg) were analyzed .Results The 2 indexes of the PT normal level and APTT abnormal level in the automatic blood coagula-tion analyzer were qualified .The PT abnormal level was fine and 3 indexes of the APTT normal level ,Fg normal level and abnormal level were excellent .Conclusion Six indexes of automatic blood coagulation analyzer could be evaluated by the method evaluation decision chart and it was more directly and clearly compared with σvalue statistical table .
9.Training of medical students' critical thinking ability in pathophysiology teaching
Longjiang LI ; Li TANG ; Yi CHE ; Yi SHEN ; Huayu DENG
Chinese Journal of Medical Education Research 2011;10(5):570-572
Carrying out the training of critical thinking in pathophysiology teaching is appropriate, and the medical students critical thinking ability can be achieved via construction of the awareness, and diverse teaching methods which include questioning, exploration and discussion.
10.Simple assessment on short term precision of XE-2100 automatic hematology analyzer
Huayu CHEN ; Yongtie GUO ; Qian LI ; Xu LIU
International Journal of Laboratory Medicine 2014;(15):2082-2083
Objective To quickly understand whether the newly introduced XE-2100 automatic hematology analyzer meets the precision promised by the manufacturer .Methods After adjusting and calibrating the new hematology analyzer ,the anticogulated blood samples by EDTA-K2 were detected for 10 times and the main parametric precisions were calculated by the Sigma unit .Results The Sigma score of this automatic hematology analyzer labeled by the manufacturer was 39 ,the actual coefficient of variation (CV) in 8 indexes was less than that labeled by the manufacturer ,the operating Sigma score was full mark 48 .Conclusion The ac-tual precision of the XE-2100 automatic hematology analyzer is preliminarily reached the precision promised by the manufacturer , the operating Sigma score is in the high quality level and achieves the expected requirements in daily use .