Ovarian cancer has the highest mortality rate among cancers of female reproductive system.Early stage of ovarian cancer shows no signs or asymptomatic and no effective screening approach is available.Soluble biomarkers represent the biomarkers existing and can be detected in the body fluid .The detection of soluble biomarkers possess the advantages of being relatively simple , minimal invasive, and low costs, thus they are very useful in screening , therapeutic response monitoring , and prognosis prediction of cancer.However, biomarkers used for ovarian cancer diagnosis are limited , and the overall sensitivity and specificity of the tests are still below clinical satisfaction currently .Thus, seeking for new ovarian cancer soluble biomarkers is current research focus.This paper discuses the features and diagnostic value of current and potential ovarian cancer biomarkers , the reasonable combination strategy in detection of ovarian cancer biomarkers in clinical practice is also discussed .

Vitamin D binding protein ( VDBP) is a multi-functional plasma globulin which binds to the vitamin D ( VD) in the circulation system and transporting the latter to various effective organs to exert its biological functions .Studies have found that VD and its carrier VDBP are associated with the risk of various cancers such as breast cancer , prostate cancer , and colorectal cancer however , lacking sufficient epidemiological evidences so far .Furthermore , it is still controversial regarding how VD controls and affects cancer progression.In addition, studies have also demonstrated that VDBP derived macrophage-activating factor (GcMAF) is a potent lymphokine in cancer treatment .Nonetheless, the relationship between VDBP and cancer including the VDBP polymorphism, VDBP plasma concentration are still unclear; more studies are needed to further uncover its role in cancer development and progression , thus providing new evidences for cancer diagnosis, treatment, and prognosis.

Objective To observe the toxicity, apoptosis and cell proliferation cycle of Mangiferin on the BEL 7404 human hepatocellular carcinoma cell line, and explore the possibility of Mangiferin as a new tumor chemopreventive drug. Methods MTT method was used to determine the effect of Mangiferin on cell proliferation, microscopy method was used to observe cytotoxicity of Mangiferin, and flow cytometry method was used to determine apoptosis induction and cell cycle proliferation blocking effects of Mangiferin. Results The cytotoxicity effects were observed in various concentrations of Mangiferin and at different exposure times. The effects were enhanced as the Mangiferin concentration increased and exposure time prolonged. The apoptosis effect was also enhanced, and cell cycle arrested at G 2/ M phase. All the above effects became significant when cells exposed to the concentration from 20 ?mol/L to 200 ?mol/L of Mangiferin at 24 h. Conclusions Mangiferin is cytotoxic, and may induce apoptosis. Mangiferin has cell cycle blocking effects on hepatocellular carcinoma cells. Its pharmaceutical potentials is worth for further investigating.

Objective To investigate the applicative value of hysteroscopic surgery in the treatment of endogenous cesarean scar pregnancy ( CSP) . Methods A total of 34 patients with CSP in this hospital from April 2012 to October 2014 were treated by hysteroscopic surgery .Seven patients received uterine curettage under hysteroscopy and 27 patients got uterine artery embolization therapy before hysteroscopic surgery .All of them were regularly reviewed of β-hCG levels and ultrasound examinations . Results The surgical procedure was successfully completed in all the 34 cases.The operation time was (17.5 ±3.5) min, the intraoperative blood loss was (17.5 ±7.5) ml, and the hospitalization stay was 4-5 days.All of them had no serious complications .Their β-hCG levels were dropped to normal range within 28 days after operation , and all the patients had menstruation within 2 months after surgery . Conclusion Hysteroscopic surgery for CSP has advantages of good visibility , less bleeding, simple performance, high success rate, less postoperative complications , and shorter hospitalization time , being worthy of clinical utilization .

BACKGROUND: Dialysis-related amyloid may occur during long-term dialysis for patients with uraemia, of which the main evocator is β_2-microglobulin (β_22M); therefore, how to eliminate 132M from blood is always the focus of research. OBJECTIVE: To observe ability of removal of β_2-microglobulin (β_2M) from serum using two kinds of polyethersulfone (PES) membrane materials with various degrees of sulfonation.METHODS: These materials were incubated in radio-labeled β_2M (~(125)Ⅰ-β_2M) solution and human serum respectively at appointed time at 37 ℃, and then the amounts of ~(125)Ⅰ-β_2M and serumβ_2M adsorbed by materials were measured by radio immunoassay. RESULTS AND CONCLUSION: In the ~(125)Ⅰ-β_2M system, amounts of ~(125)Ⅰ-β_2M adsorbed by the materials decreased in the following sequence PES with high degree of sulfonation > PES with low degree of sulfonation > PES, whatever the source of PES was. In the serum system, amounts of β_2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES with high degree of sulfonation > PES with low degree of sulfonaUon > PES. Sulfonated PES removed β_2M more than PES did and the adsorption of β_2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of β_2M may result in less β_2M available for incorporation into amyloid. The use of sulfonated PES membranes may lessen the likelihood of development of dialysis-related amyloidosis, which remains a major source of morbidity for patients treated with long-term hemodialysis.

This study was performed to evaluate the adsorption of beta2-microglobulin(beta2 M) by blood dialysis membrane materials which are polyethersulfone (PES), sulfonated polyethersulfones, (PES-SO3Na-I and PES-SO3Na-I ) in vitro incubated in human serum and radiolabeled beta2M (125I-beta2 M) solution respectively. In these experiments, the materials were incubated in 125I-beta2 M solution and human serum at the appointed time ranging from 15 minutes to four hours at 37 degrees C, and then the amounts of 125I-beta2M and serum beta2M adsorbed by materials were measured by radioimmunoassay (RIA). In the 125I-beta2 M system, amounts of 125I-beta2M adsorbed by the materials decreased in sequence of PES-SO3 Na-II > PES-SO3Na-I > PES. In the serum system, amounts of beta2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES-SO3Na-II > PES-SO3Na-I > PES. Sulfonated PES removes beta2M more than PES does and the adsorption of beta2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of beta2M may result in less beta2M available for incorporation into amyloid. The use of PES-SO3Na membranes lessens the likelihood of dialysis-related amyloidosis (DRA) development, which remains a major source of morbidity for patients treated with long-term hemodialysis.
Adsorption ; Amyloidosis ; blood ; Humans ; Polymers ; chemistry ; Renal Dialysis ; adverse effects ; Sulfones ; chemistry ; beta 2-Microglobulin ; blood

Objective To explore the predictive value of different CT signs in surgical treatment in patients with acute closed intestinal and mesenteric injury.Methods The CT data and follow-up results of 120 patients with acute closed intestinal and mesenteric injury(including 58 cases of surgical treatment and 62 cases of conservative treatment)were analyzed retrospectively.The diagnostic value and prognostic value in surgical treatment of different CT signs were analyzed.Results There were significant differences between the operation group and the conservative treatment group in mesenteric vascular amputation,mesenteric hematoma,vascular contrast agent extravasation, intestinal wall defect,pneumoperitoneum and peritoneal effusion (χ2＝4.69,P＝0.03;χ2＝21.40,P＜0.001;χ2＝13.95,P＜0.001 ;χ2＝11.34,P＝0.001;χ2＝80.65,P＜0.001).The CT signs of intraperitoneal effusion had the highest sensitivity for intestinal and mesenteric injury,and vascular contrast agent extravasation and intestinal wall defect had the highest specificity for surgical predictability of intestinal and mesenteric injury.ROC curves showed that abdominal gas accumulation had the highest predictive value for intestinal and mesenteric injuries (AUC＝0.92,95%CI:0.87－0.97),followed by mesenteric hematoma (AUC＝0.73,95%CI:0.64－0.82).Conclusion Intraperitoneal effusion has the highest sensitivity for intestinal and mesenteric inj ury,and vascular contrast agent extravasation and intestinal wall defect have the highest specificity for surgical predictability of intestinal and mesenteric injury.The overall value of intraperitoneal gas in predicting intestinal and mesenteric injury is highest.Combining different CT signs can improve the accuracy of clinical intervention for acute closed intestinal and mesenteric injury.

OBJECTIVETo raise awareness of the pathogenesis and diagnosis of thalassemia by reporting one case of α thalassemia patient with a large deletion fragment and analyzing the pedigree.

METHODSFirstly, blood cells and hemoglobin electrophoresis analysis were used for screening of thalassemia, and then three common kinds of deletional α thalassemia in Chinese was detected by Gap-PCR, three common kinds of non- deletional α thalassemia and seventeen common mutations of β thalassemia in Chinese were analyzed by using PCR- RDB. The unknown mutation of samples was identified with Multiplex Ligation-dependent Probe Amplification (MLPA) and DNA sequencing.

RESULTSThe proband female presented with microcytic hypochromic anemia(hemoglobin 71 g/L, mean corpuscular volume 52.4 fl, mean corpuscular hemoglobin 16.1 pg), and hemoglobin A2 1.4%. The identified large deletion fragment length was 21 925 bp, so far which had not been reported in the world and was named -α²¹·⁹. It was registered in USA DNA database and GenBank accession number as KF360979. The genotype of her mother and father and brother were αα/-α²¹·⁹, --(SEA)/-α³·⁷, αα/-α³·⁷ respectively, and the genotype of her and her sister were the same of --(SEA)/-α²¹·⁹. Her husband gene of thalassemia had no mutation, so prenatal diagnosis of thalassemia was not carried out in the pregnant woman.

CONCLUSIONThe discovery of -α(21.9) deletion mutation was enriched the DNA mutation gene database of thalassemia, and had important significance for genetic counseling and thalassemia prenatal diagnosis.