To study the relationship between angiotensin converting enzyme(ACE) gene polymorphism and angiotensin converting enzyme activity,the polymerase chain reaction(PCR) was used to detect an insertion/deletion(I/D) polymorphism of ACE gene in its sixteenth intron.The genotype,enzyme coupling spectrophotometry were used to measure serum ACE activity among the three genotypes.Results showed that the Serum ACE activities were (337 6?92 0),(292 7?76 5 )and(243 5?58 9)u/L for genotypes DD,ID and ⅠⅠ respectively.The findings indicate that the ACE gene polymorphism closely associated with serum ACE activity.

Objective To investigate the clinical significance of HCV core antigen (HCV-cAg )in hepatitis C screening. Methods From October 2014 to October 2015 ,a total of 8000 serum samples were collected from the outpatient and inpatient. HCV-cAg and hepatitis C antibody (HCV-Ab) were detected by using ELISA.The positive samples of HCV-cAg or HCV-Ab were performed the confirmative diagnostic detection by PCR.Results Among 8000 serum samples ,82 positive samples were screened out ,in which 73 cases were HCV-RNA positive by elementary screening with the standard of HCV-cAg or HCV-Ab positive ,a-mong which 72 were HCV-RNA positive and and 9 cases were HCV-RNA negative by HCV-RANA confirmation.The sensitivity and specificity of HCV-cAg were 45.83% and 99.98% ,sensitivity and specificity of HCV-Ab were 94.44% and 99.90% ,which of HCV-cAg and HCV-Ab combined detection were 100.00% and 98.86% respectively.Conclusion HCV-cAg and HCV-Ab play complementary role in HCV screening.Their combined detecting contributes to increase the screening rate of HCV.

Objective Apolipoprotein E(ApoE) gene polymorphism and alzheimer and coronary heart disease was studied ,and the correlation between them was evaluated.Methods The polymerase chain reaction-restriction fragment length polymorphism was used to amply 227 base-pari DNA fragment in the fourth exon of ApoE gene,and digested them with restriction enzyme CfoI,electrophoresis on 8% polyacrylamaid gel for the cleavaged products,subsequently genotype was determined by silver staining and allele frequencies were counted.Results apoE genotype and allele frequencies was detected in 33 case with Alzheimer and 37 case with coronary heart disease.The results showed that the frequencies of ApoE ? 4 was significantly higher than that in age-matched controls(P

Objective To introduce three new methods for measuring breast volume. Methods The breast volume was measured and the 3D image yielded with a special software or calculated with two mathematical formulas, according to its base, horizontal and saggital sections. Results The average deviations of these protocols were less than 10 % of the actual sizes in our investigation. Conclusion The computer aided measurement for the volume of the breast is an accurate and reliable method and can be used in the designs for augmentation and reduction of mammaplasty.

Objective The relationship among angiotensin converting enzyme(ACE) gene polymorphism,angiotensin converting enzyme activity and coronary heart disease(CHD) was studied.Method Teh polymerase chain reaction was used to detect an insertion/deletion(I/D) of ACE gene polymorphism in the sixteen intron,then genotype and allele freqencies were counted .The method of enzyme coupling ratio was used to detect ACE activity .Results In 51 case with CHD ,the DD genotype and D allele freqencies of ACE gene were 35% and 61% respectively ,ACE average activity was (350 3?91 1)su/L.In 83 normal control subject,they were 16% and 45% and (286 7?79 6)su/L respectively.The freqencies of DD genotype and D allele and ACE average activity had significant difference.Conclusion The deletion polymorphism of ACE gene might be an latent risk factor.The higher ACE activity might play an important role in the development of CHD.

This paper briefly introduces the research background, combined with the practical of project, analysis the feature of the construction of information collection system of TCM clinics, and puts forward how to use the method of project management in the construction of information collection system of TCM clinics. By exploring the Project Phasing, Work Breakdown Structure, Project Responsibility Matrix, and Project management standard system, it hopes to strengthen the awareness of project management in the construction of information collection system of TCM clinics, and to guide the project management and the system construction.

Objective To investigate the relationship between the change of blood flow in exceedingly expanded skin and the viability of the flaps. Methods In 21 expansions of 10 patients, the skin blood flow and its amplitude were measured by laser Doppler in different stages. The results were compared with the final outcomes of patients. Results The volume of blood flow and its amplitude of normal skin were very low and stable, but after expanded, the volume of blood flow and the amplitude grew up, which were significantly higher than that of normal skin (P

Aim To investigate the effects of emodin isolated from Guangxi P.multiflorum Thunb on the expression of KU70/KU80 in hypoxic nasopharyngeal cancer CNE-1 cells and reveal the relationship between radiosensitization of emodin monomer and DNA repair genes.Methods The expression of hypoxia inducible factor-1?(HIF-1?)and DNA double-strand break repair genes(KU70/KU80)between the experimental groups and the control group under hypoxic condition was detected by the real-time fluorescence quantitative RT-PCR.Results Expression of HIF-1? was significantly increased under hypoxia condition.HIF-1? had no change after treatment with emodin alone.The expression level of KU70/KU80 was induced by radiation.Compared with radiation alone group,radiation combined hypoxia group obviously enhanced the expression of KU70/KU80.KU70/KU80 mRNA expression significantly reduced after radiation combined with emodin under hypoxic condition.Conclusion In the hypoxic environment,emodin combined with radiotherapy can effectively inhibit the expression of HIF-1 ? and DNA double-strand break repair genes(KU70/KU80),which may be its mechanism of radiosensitization.

Objective:To investigate the effect of different ventilation time in the prone position on patients with endogenous/exogenous ARDS.Methods:30 endogenous/30 exogenous ARDS patients were randomly devided into 4 groups,ventilation in the prone position for 2 h and 4 h.Recording the score of APCHEII,oxygenation index,the absorption situation in X-ray,HR,MAP,extubation time,the time out of ICU.Results:The APCHEII scores HR and MAP in four groups have no significant statistics (P＞0.05);4h ventilation for endogenous ARDS patients has a better indicators than 2 h in oxygenation index,the absorption situation in X-ray,extubation time and the time out of ICU (P＜0.05);2 h and 4 h ventilation for exogenous ARDS patients can improve indicators above,two groups have no significant statistics (P＞0.05),the results of exogenous groups are precede than endogenous group (P＜0.05).Conclusion:Ventilation in the prone position can improve the situation of ARDS patients,both endogenous patients and exogenous patients.Exogenous ARDS patients have a better treatment effect after the ventilation of 2h,however,endogenous patients need longer time and have a non-ideal prognosis.

Objective To study the inhibitory effect of arsenic trioxide(As_2O_3) on the tumor growth of breast cancer cell line MCF-7 implanted subcutaneously in nude mice and its mechanism.Methods BALB/C-nu/nu nude mice were subcutaneously injected with MCF-7 breast cancer cell line,and treated with intraperitoneal injection of As_2O_3 and 5-FU in different concentrations.The implanted tumor was weighed,and the tumor inhibition rates were calculated.The apoptosis of the implanted tumor was detected by flow cytometry.The expressions of bcl-2 and Fas induced by As_2O_3 were examined by immunohistochemical method.Routine blood test and bone marrow test were used to observe the function of hematopoietic system after As_2O_3 treatment.Results The growth of implanted tumor was markedly inhibited with 5-FU,low dose and high dose As_2O_3,the inhibitory rates being 38.33%、51.42% and 62.43%,respectively.The inhibitory effect of As_2O_3 was significantly stronger than that of 5-FU(P