Objective To explore the experiment of Mg2+on effects of cell apoptosis of rabbit secondary brain injury. Methods Fifty New Zealand white rabbits, irrespective of genders were selected, they were randomly divided into ex-perimental group, control group and operation control group, 20 rats in each group of the experimental group and the control group, 10 rabbits were in the sham operation group; animal were used magnesium sulfate after brain injury in the early vein,through the pathology examination of brain tissue around contusion foci, X-ray examination of electron microscopy and cell apoptosis detection, the effect of magnesium ion on the cell apoptosis in secondary brain damage was analyzed. Results The control group after 6 h of brain injury, pathology showed focus of the lesions appeared haem-orrhage, the organization gap widened, peripheral edema, 48 h after brain injury was most significant, the lesions ap-peared cell membrane structure complete, the nucleus pycnosised nerve cells,that was to say, neural cell apoptosis, apoptotic cells were relatively dense; Neural cell degeneration and necrosis of experimental group were less , brain edema was lighter, there were no significant differences on both sides of the lesions; the expression of TUNEL positive cells in normal brain tissue was less, 6-hour-injury specimens appeared TUNEL powder in a small number of positive cells, the number of TUNEL positive cells of specimens of two groups increased with the prolongation of injury time, the peak period was 24 hours, TUNEL positive cells at different stages in the experimental group were significantly lower than those of the control group at the same time segment, the difference had statistical significance (P<0.05). Conclusion Magnesium ions can reduce the number of nerve cell apoptosis of rabbit secondary brain injury, play a pro-tective role in injury of cerebral nerve cells.

Objective To investigate the relationship between serum secreted phospholipase A2 (sPLA2) and the severity of the disease in cobra bite patients. Methods Fifty-two cobra bite patients admitted to our hospital from January 2015 to December 2015 were selected according to the inclusion and exclusion criteria. According to the degree of disease, patients were divided into the light and heavy risk groups. According to the degree of swelling of the affected limbs, patients were divided into the mild swelling group and moderate-severe swelling group. According to the time difference between visits, patients were divided into: visit time <8 h group and visit time ≥ 8 h group. Twenty healthy adult volunteers served as the control group. The levels of serum sPLA2, C reactive protein (CRP), D-dimer (DD), lactic acid (LAC) and peripheral blood leukocyte count (WBC) were measured and compared in different groups. Quantitative data were analyzed using t test and rank sum test. Results The levels of serum sPLA2 in the light and heavy risk group were statistically different from those in the control group (P<0.05), and were statistically significant between the light and heavy risk groups (P<0.01) and between the mild swelling group and moderate-severe swelling group (P<0.05). The serum sPLA2 levels in group A and group B were also significantly different from those in the control group (P<0.05). The level of sPLA2 was positively correlated with the level of LAC, DD and WBC, and the correlation coefficients were 0.3142, 0.2752 and 0.6534, respectively. Conclusions The higher the level of serum sPLA2, the more serious the patient's poisoning symptoms. It is of certain clinical value to evaluate the condition of cobra.