1.Determination of adenosine in Ligia exotica with ultraviolet spectrophotometry
Weidong XUAN ; Jun BIAN ; Huawu ZENG ; Jing XEAO
Chinese Journal of Marine Drugs 1994;0(01):-
Objective To establish a UV method of determination of adenosine in Ligia exotica. Methods Adenosine was extracted with 37℃ water and separated by TLC silica gel, the absorbance was detected at 260nm with UV spectrophotometry.Results The cabibration curve for adenosion was linear within the range of 1.0~20.0?g?mL -1, r=0.9998 (n=6), the average recovery was 99.3%,RSD=1.9% (n=3). Conclusion The method was simple, convenient and accurate for determination of adenosine in Ligia exotica.
2.Elects of CI-930 plus PGE,or SQ-22536 on Arachidonic Acid Metabolism and cAMP Con-tents in Platelets
Huawu ZENG ; Ximheng CHEN ; Yuanying JING ; Kun LONG
Academic Journal of Second Military Medical University 1981;0(03):-
The contents of cAMP and (14C)-AA metabolites in rabbit platelets were measured with RIA and radioactive TLC, respectively. PGE, could cooperate with the effect of CI-930. on platelets to increase the cAMP contents. It had no influence on the effect of CI-930 on metabolites of (14C)-AA, at the same time. SQ-22536 100?mol?L-1 could not chang the effect of CI-930 on platelets, which increase cAMP contents and affect AA metabolism. The date suggest that there is not relation between the effect of CI-930 on AA metabolism and on the cAMP contents in platelets.