Diagnosis of sphenoid sinus disease is very difficult because the location of sinus is deep and hidden within the skull and the symptoms of sphenoiditis are nonspecific. However, thanks to new technologies in imaging (CT and MRI) and nasal endoscopy, the literature on sphenoid sinus fungus ball have been published more. But all of the SSFB which have been reported are isolated or unilateral. We reported one rare case of bilateral sphenoid sinus fungus balls. This patient was treated in our department. Headache was the only symptom in this case. The patient was treated by sphenoidotomy via endoscopic approach and removal both of the lesions. No recurrence was found after 6-months follow-up.
Adult ; Humans ; Male ; Mycoses ; pathology ; Sphenoid Sinusitis ; microbiology

Objective:To investigate therapeutic effect of reinfusion of tumor-infiltrating lymphocyte(TIL)with clustered regularly interspaced short palindromic repeats/CRISPR-associated 9(CRISPR/CAS9)knockout programmed death-1(PD-1)on colon cancer in mice.Methods:Subcutaneous injection of CT26 was used to establish mouse colon cancer model.TIL was extracted from tumor tissue of three model mice,and peripheral blood lymphocytes were extracted.PD-1 gene was knocked out of TIL.Reinfusion experiments were divided into control group(Control),lymphocyte group(Lym),tumor-bearing mouse TIL group(TIL),lentivirus empty empty group(pVSV-G-PX458-NC)and PX458-PD-1-sgRNA1 group(PD-1-sgRNA1),with 10 mice in each group.Tumor tissue quality and tumor inhibition rate were detected in each group.TUNEL was used to detect cell apoptosis in tumor tissues of mice.ELISA was used to detect contents of TNF-α and IFN-γ in tumor tissues of mice.Immunohistochemistry was used to detect expressions of CD4+T and CD8+T cells in tumor tissue.Immunofluorescence was used to detect expressions of proliferating cell nuclear antigen-67(Ki-67)and vascular endothelial growth factor(VEGF).Western blot was used to detect expressions of PD-1 and its ligand PD-L1 in tumor tissues.Results:PD-1-sgRNA1 could significantly inhibit growth of mouse tumor cells in vivo,inhibit expressions of Ki-67 and VEGF in tumor tissues,as well as expressions of PD-1 and PD-L1,elevate apoptosis rate,contents of TNF-α and IFN-γ in tumor tissues,and expressions of CD4+T and CD8+T cells(all P<0.05).Conclusion:Reinfusion of TIL with CRISPR/CAS9 knockout PD-1 can significantly inhibit expressions of Ki-67 and VEGF in colon cancer mice,enhance infiltration of CD4+T and CD8+T cells,induce tumor cell apoptosis and inhibit tumor growth.

Objective:To investigate the factors associated with complications following the implantation of external-expansion devices with an injection port.Methods:A retrospective study was conducted, including 68 patients who underwent expansion device implantation at the Department of Plastic Surgery, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, from January 2019 to May 2022 and the patients experienced postoperative complications (case group). Additionally, 195 patients who underwent the same procedure during the same period but did not experience complications were selected as control group, following a 1∶3 ratio. Preoperatively, different shapes and sizes of expansion devices were chosen according to the lesion location and area. Observational indicators included admission diagnosis, preoperative white blood cell count, preoperative hemoglobin count, gender, age, implantation site of the expander, season of admission, and duration of postoperative drainage tube placement. Univariate and multivariate logistic regression analyses were used to analyze factors associated with postoperative complications.Results:Univariate logistic regression analysis revealed that implantation at the trunk ( OR=0.439, 95% CI: 0.207-0.901, P=0.028), admission diagnosis of microtia ( OR=4.155, 95% CI: 1.735-10.206, P=0.001), and admission season from April to September ( OR=3.269, 95% CI: 1.819-6.073, P<0.001) were associated with complications following expansion device implantation. Multivariate logistic regression analysis showed that admission season from April to September ( OR=3.272, 95% CI: 1.796-6.156, P<0.001) was a significant factor associated with postoperative complications. Conclusion:Implantation site at the trunk, admission diagnosed with microtia, and admission season from April to September are the factors associated with complications following expansion device implantation.