1.Effect of Quickread-bioindicator: An Observation
Shusen LIANG ; Huasheng WANG ; Ran XU
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To observe the effect of the quickread bioindicator. METHODS According to the GB15981-1995 The Measures and the Standards of the Assessment of Disinfection and Sterilization,to control conventionally the(7 high-pressure) steam sterilization cabinets by the quickread-bioindicators. RESULTS Using the effective control method shortened the waiting time by 45 hours,and in-time provided the qualified disinfected supply. CONCLUSIONS The effect of using the quickread-bioindicator resolves the problem of delay of the control result.This is an ideal,scientific,and practical method for effective control of the high pressure steam sterilization.
2.A Whole Range Quality Control of Disposable Sterile Medical Appliance by Supply Room
Xueying SUN ; Huasheng WANG ; Ran XU
Chinese Journal of Nosocomiology 2006;0(09):-
OBJECTIVE To investigate the procedures and advantages of whole range quality control of disposable sterile medical appliance. METHODS By analyzing the data concerning whole range quality control of disposable sterile medical appliance from Oct 2002 to Oct 2005 in our hospital to summarize the experiences in this field. RESULTS During the last 3 years, the supply room in our hospital had administrated disposable sterile medical appliance 30 069 000 articles altogether, with the qualification ratio of 100%, and no adverse reaction was found; furthermore, the waste was reduced for no massed articles occurred; the cost accounting was raised and the error ratio during delivery was reduced too, the annual error ratio dropped form 0.003% to 0.00066%. CONCLUSIONS The whole range quality control of disposable sterile medical appliance can raise the administration quality, reduce the waste during delivery, and guarantee the disposable sterile medical appliance being used safely and effectively, also, it can provide accurate and useful data of cost accounting to concerned departments.
3.Occupational Harmful Factors and Protective Measures in Department of Disinfection and Supply
Xueying SUN ; Huasheng WANG ; Ran XU
Chinese Journal of Nosocomiology 2006;0(08):-
OBJECTIVE To analyze occupational harmful factors in department of disinfection and supply and take protective measures in pertinency.METHODS We analyzed every tache of work flow in the course of disinfection and supply,and found out the potential occupational harmful factors.Based on them,the easy and feasible protective measures were proposed.RESULTS There existed many occupational harmful factors in the course of disinfection and supply.Therefore,the working environment and occupational protection among staff should be improved.CONCLUSIONS Analysis on the peril of occupational harmful factors,enforcement of protective consciousness and establishment and implementation of operation rules and protective measures are important steps of occupational safety management for staff in department of disinfection and supply.
4.Effect of Batch Monitoring System During Sterilization Process
Huasheng WANG ; Yuhua GAO ; Ran XU
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To verify the effect of batch monitoring system insteod of the indicator in the package.METHODS From Apr 17,2007 to Jul 29,2008,the batch monitoring system was used to monitor the sterilization effect of each batch,at the same time,inside each package we placed the indicator as control.RESULTS A total of 7057 batches were monitored,in which 7051 were eligible,and among 166870 packages in all,the indicators were all eligible,which included 56600 dressing packages and 110270 instrument packages.There was no difference between batch monitoring system and indicators.CONCLUSIONS The effect of batch monitoring system is credible,and the indicator inside package can be replaced.
5.Effects of deoxycytidine on DNA methylation state and expression of mRNA and protein of pl6 gene in human squamous lingual carcinoma SCC-9 cells
Huasheng XU ; Xuanping HUANG ; Xi LIN
The Journal of Practical Medicine 2016;32(16):2613-2616
Objective To study the effects of deoxycytidine (5-aza-2 deoxycytidine, DAC) on DNA Methylation state and expression of mRNA and protein of pl6 gene in human squamous lingual carcinoma SCC-9 cells in vitro. Methods The SCC-9 cells were divided into four groups, group 0, 1, 2 and 3 which processed using three gradients concentration of DAC. The group 0 without DAC was as the control group. Q-MSP was used to detect the state of methylation of the p16 in SCC-9 cells treated by DAC after 48 hours. Real-time fluorescence quantitative PCR was used to detect mRNA expression level changing of the p16 in SCC-9 cells treated by DAC. Immunohistochemical method was used to detect the expression of p16 protein. Results The hypermethylation and non-methylated p16 gene in SCC-9 was mixed with the results of Q-MSP. The results of Real-time PCR showed that mRNA expression of p16 in SCC-9 cells which treated by the different concentration of DAC for 48 hours was higher the control group. And difference was statistically significant (P < 0.05). The high expression of p16 protein was found in the experimental group with immunohistochemical method. Conclusion The p16 gene methylation states of SCC-9 may be suppresses and the recovery of mRNA and protein expression of p16 gene must be prompted by DAC.
6.RFID Information Management Technology Applying in CSSD
Huasheng WANG ; Ran XU ; Shusen LIANG ; Wei XU
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To build up an integrated,flexible,real-time backward management system for recycling medical appliance.METHODS The radiofrequency identification technique(RFID) information management technology was adopted.RESULTS Exertion of RFID information management technology in Central Sterile Supply Department(CSSD) not only promoted its work more effectively,correctly and feasibly,but also guaranteed the quality of recycling medical appliance.CONCLUSIONS The RFID information management technology applying in CSSD,could control the whole process of managing recycling medical appliance backward completely,and gain the legal witness and humanity management.
7.Washing Quality for Medical Instruments and Its Impact Factors
Huasheng WANG ; Xueying SUN ; Shusen LIANG ; Ran XU
Chinese Journal of Nosocomiology 2006;0(05):-
OBJECTIVE To explore the impact factors on the process of washing and disinfection for medical instruments.METHODS The reasons that tend to cause bad washing quality were analyzed in the process of washing for medical instruments and corresponding measures were taken.RESULTS Efficient washing procedures should be installed in the process of washing for medical instruments and washing effects should be affirmed.CONCLUSIONS Efficient washing methods should be taken to guarantee successful sterilization and control hospital infection.
8.Reversal effect of FG020318 on multidrug resistance in retinoblastoma cell line SO-Rb50
Huasheng YANG ; Guoying LIU ; Juanjuan XU ; Ping ZHANG ; Danping HUANG
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: To investigate the multidrug resistance(MDR),reversal activity of 2-[4-(2-pyridin-2-yl-vinyl) henyl]-4,5-bis-(4-N,N-diethylaminophenyl)-1(H)-imidazole(FG020318) in a retinoblastoma subline SO-Rb50/VCR,which is resistant to vincristine.METHODS: The procedure of stepwise increase in drug concentrations was used to obtain SO-Rb50/VCR,which was resistant to 200 ?g/L vincristine. The chemosensitivity of this drug resistant cell line with and without FG020318 or cyclosporine A(CSA) were detected by MTT assay and the function of p-glycoprotein(P-gp) was examined by rhodamine 123 accumulation detected with flow cytometry(FCM).RESULTS: FG020318(2.5 ?mol/L) significantly reduced IC50 and increased the rhodamine accumulation in a concentration-dependent manner. It was much stronger than the positive control CSA in reversal of MDR.CONCLUSION: A new tumor MDR modulator FG020318 partly reverses MDR in SO-Rb50/VCR.It may be a promising new drug to tackling MDR.
9.Clinical study on treatment of patients with upper digestive tract malignant tumors of middle and late stage with Ginkgo biloba exocarp polysaccharides capsule preparation
Huasheng CHEN ; Fan ZHAI ; Yunfei CHU ; Fang XU ; Aihua XU ; Linchang JIA
Journal of Integrative Medicine 2003;1(3):189-91
OBJECTIVE: To observe the clinical efficacy of Ginkgo biloba exocarp polysaccharides (GBEP) capsule preparation in treating upper digestive tract malignant tumors of middle and late stage. METHODS: Eighty-six patients of the upper digestive tract malignant tumors were treated with GBEP capsule preparation taken orally. The clinical symptoms and the qualities of life of the patients with single GBEP and combined with operation, radiotherapy or intervention chemotherapy were observed. The tumor size was measured by electronic gastroscope before and after treatment with single GBEP. Objective response rate (RR) of the tumor was calculated. The survival period of patient was observed. The changes of blood routine examination in the patients treated with radiotherapy were observed. RESULTS: GBEP preparation could markedly improve the patients'clinical symptoms. Karnofsky scoring of the patients markedly increased after treatment. There were 2 CR (complete response, 6.3%), 22 PR (partial response, 68.8%)and 5 SD (stable disease, 15.6%) of 32 cases with single GBEP preparation. The survival periods of the 32 cases were markedly prolonged. The preparation could relieve the inhibited hematopietic function and the weight loss due to radiotherapy. CONCLUSION: GBEP capsule preparation has some definite therapeutic effects on upper digestive tract malignant tumors of middle and late stage.
10.Dysphagia after radiotherapy:esophageal barium fluoroscopy examination of swallowing in nasopharyngeal carcinoma patients
Fengjie LIN ; Luying XU ; Huiqin CHEN ; Huasheng LI ; Sufang QIU ; Shaojun LIN ; Cairong HU ; Jun LU
China Oncology 2015;(5):371-376
Background and purpose:Currently, subjective questionaire is the most frequently used methods to evaluate swallowing dysfunctions after radiotherapy in nasopharyngeal carcinoma patients, while lacking of effective objective examinations. This study aimed to explore effective methods to evaluate swallowing dysfunctions after radiotherapy in nasopharyngeal carcinoma patients, and gain knowledge of the incidence and severity of swallowing dysfunctions. Methods: From Oct. 2013 to Dec. 2013, 128 consecutive outpatients with previously treated nasopharyngeal carcinoma received esophageal barium lfuoroscopy examination at there regularly follow-ups to evaluate swallowing function. Among these patients, 89 were primary treated with intensity modulated radiation therapy (IMRT) and 39 with conventional radiotherapy (CRT). In this study, each patient received esophageal barium lfuoroscopy examination for 3 times with thin, thick and pasty barium and were dynamically observed using X-ray fluoroscopy from front and lateral direction. Swallowing dysfunctions were defined as follows:①The bolus could not be swallowed and blocked in the mouth;②The dilute barium diverted to the glottis or trachea;③Residual barium delayed in the pyriform sinus and vallecula;④The movement of the hyoid bone or epiglottis were restricted;⑤Bolus prolong through the pharynx;⑥Barium slowed down when went though the esophageal entrance. Results:Of the 128 patients, incidence of dysphagia was 60.2%for the entire cohort, 52.8%for IMRT group and 76.9%for CRT group. Incidence of dysphagia for IMRT group was signiifcantly lower than CRT group (P=0.018). Dysphagia incidence within 1 year, 1 to 2 years and more than 2 years after RT were 63.1%, 33.3%and 69.0%, respectively (P=0.019). Conclusion:There was a high incidence of swallowing dysfunction for the nasopharyngeal carcinoma patients treated with radiotherapy and dysphagia incidence decreased when treated with IMRT. Esophageal barium lfuoroscopy examination is objective method to evaluate the incidence and severity of the swallowing dysfunction.