Objective To study the changes of serum vascular endothelial growth factor(VEGF)levels before and after interventional therapy of hepatocellular carcinoma(HCC),in order to find out a experimental proof that can start the anti-angiogenesis therapy.Methods Enzyme linked immunosorbentassay(ELISA)was used to detect serum levels of VEGF before transcathter arterial chemo-ebolization and in 24 hours,72 hours,7 days,14 days,30 days,after the operation,in 50 healthy individuals and 54 HCC pationts.Results Serum levels of VEGF in 54 patients with HCC were significantly higher than those in healthy individuals(P

OBJECTIVE To prove the batch monitoring system to be effective and practicable.METHODS The sterilization process of each batch with the batch monitoring system was monitored.RESULTS Among monitored 7057 6 batches failed,the failure rate was 0.09%.CONCLUSIONS By analyzing the failure causes,we should enhance the maintenance of the sterilizator and limit the using times of the monitor device.It proves that the batch monitoring system is effective and practicable to monitor the pulse-vacuum-pressure-steaming sterilizator.

OBJECTIVE To explore the methods of centralized disinfection for bed units in the hospital.METHODS Choose the appropriate methods to disinfection the bed units,make the basic condition,set up the administration rules and validation disinfection process.RESULTS The centralized disinfection for bed units could supply inpatients safety,clear and comfortable treatment environment and living space.CONCLUSIONS Applying the centralized disinfection for bed units is the necessary tendency for modern hospital.

OBJECTIVE To verify the effect of batch monitoring system insteod of the indicator in the package.METHODS From Apr 17,2007 to Jul 29,2008,the batch monitoring system was used to monitor the sterilization effect of each batch,at the same time,inside each package we placed the indicator as control.RESULTS A total of 7057 batches were monitored,in which 7051 were eligible,and among 166870 packages in all,the indicators were all eligible,which included 56600 dressing packages and 110270 instrument packages.There was no difference between batch monitoring system and indicators.CONCLUSIONS The effect of batch monitoring system is credible,and the indicator inside package can be replaced.

Objective To screen down regulated genes and find down regulated novel genes in gastric cancer. Methods Genes mRNA expression were detected between gastric cancer and normal gastric mucous membrane of five patients using cDNA microarray. Genes mRNA expression signals on hybridization membranes were analysized with computer software. Down regulated genes in gastric cancer were screened. cDNA suppression subtraction library was established by counterpart normal gastric mucous membrane mRNA(Tester) subtracting gastric cancer tissues mRNA(Driver) of five patients. After identification of the subtraction library, positive clones choosen randomly were sequenced , and down regulated novel genes in gastric cancer were screened. Some of the genes were identified by RT PCR.Results Down regulated genes in gastric cancer consist of 60 genes including tumor suppressing genes, apoptosis related genes, DNA replication and transcript or translate related genes, cell cycle related genes, cell migration related genes, etc. Two unknown gene fragments in gastric cancer were cloned. Conclusions Sixty down regulated genes in gastric cancer are confirmed. They are involved in gastric tumorigenicity and metastasis. cDNA subtraction library of gastric cancer was constructed successfully. Two down regulated novel gene fragments in gastric cancer was found.