OBJECTIVE:To evaluate the current situation and the trend of NSAIDs(Non-steroidal antiinflammatory dr_ ugs).METHODS:NSAIDs used in16hospitals in Wanzhou area were statically analyzed with a reference to the DDDs(defind_ ed daily dose).RESULTS:The consumption sum and DDDs increased with an annually rate of2.4%,3.1%respectively.Of the top10manufacturers in sales volume,the percentage for the sales volume of the domestic manufacturers decreased from62%in2001to24%in2003.CONCLUSION:Effort should be taken for the development of the new preparation of NSAIDS that of low side-effects so as to meet the needs of the patients.

0.05). CONCLUSION: The carbohydrate diet can not influence on pharmacokinetics parameters of gatifloxacin.

OBJECTIVE:To study the effect of high protein diet on pharmacokinetics of gatifloxacin.METHODS:A randomized crossover design was performed in 8 Beagle dogs.In the two study periods,a single oral 17 mg?kg-1 dose was administered to empty stomach or with high protein diet in dogs.Plasma concentration of gatifloxacin were measured by HPLC.The pharmacokinetic parameters were calculated by 3p97.RESULTS:The concentration-time curves were a one compartment model in empty stomach or with high protein diet in beagle dogs.The t1/2ke were(4.00?0.84)and(9.28?1.32)h,tmax(2.29?1.08)and(4.98?2.76)h,Cmax(6.06?0.87)and(3.42?1.20)?g?mL-1,AUC(0～t)(56.74?10.80)and(68.66?15.92)?g?h?mL-1,respectively.There was significant difference between the two groups in the main pharmacokinetic parameters(P

OBJECTIVE: To study the effect of lipid diet on the pharmacokinetics of gatifloxacin.METHODS: 8 Beagle dogs were randomly assigned to receive single oral dose of gatifloxacin capsules(17 mg?kg-1) on empty stomach or in combination with lipid diet by a randomized crossover design plasma concentrations of gatifloxacin were measured by HPLC.The pharmacokinetic parameters were calculated by 3p97,and the effect of the lipid diet on the pharmacokinetics of gatifloxacin was evaluated.RESULTS: The concentration-time curves fitted one compartment model in empty stomach group and lipide diet group.The main pharmacokinetic parameters were as follows: t1/2ke were(4.00?0.84) and(7.87?1.54) h;tmax(2.29?1.08) and(3.61?2.72) h;Cmax(6.06?0.87) and(2.91?1.27) ?g?mL-1;AUC(0～t)(56.74?10.80) and(45.35?10.81) ?g?h?mL-1,respectively There were significant differences between the two groups in the t1/2Ke and Cmax(P

OBJECTIVE: To survey and objectively evaluate the present situation and trend of the usages of the prescription drugs and over the counter( OTC) drugs. METHODS: To make a survey of the usage for above - mentioned drugs in 51 hospitals of 4 provinces in southwest China during the period 1999- 2000 concerning the sum of money for drugs, main kinds of drugs etc. . RESULTS: The sum of money for the prescription drugs and OTC drugs increased 55% and 33% in 2000 compared with those in 1999, respectively.The sums of money for OTC drugs accounted for 2.18% and 2.56% of those of the prescription drugs in 2000 and in 1999,respectively.CONCLUSION:The sums of money for the prescription drugs and OTC drugs increased rapidly ,but total sale of OTC drugs was very low compared with that of the prescription drugs.

Objective To learn the correlation between thyroid function and arteriosclerosis in elderly popu-lation,and to provide research data for early clinical intervention.Methods 7 1 elderly patients with hypothyroidism were selected as A group,76 elderly patients with subclinical hypothyroidism were selected as B group,70 healthy elderly cases were selected as C group.Triiodothyronine (FT3 ),tetraiodothyronine (FT4 ),thyrotrophin (TSH ), triglyceride (TG),total cholesterol (TC ),low density lipoprotein cholesterol (LDL -C ),high density lipoprotein cholesterol (HDL-C),C reactive protein (CRP)levels of the three groups were detected.Carotid artery intima media thickness (CIMT)was examined by color Doppler ultrasonography.Results FT4 levels of A group,B group were (5.25 ±4.17)pmol/L,(10.74 ±5.02)pmol/L respectively,which were lower than (18.53 ±6.38)pmol/L of C group.TSH levels of A group,B group were (16.45 ±6.96)mU/L,(6.25 ±3.84)mU/L respectively,which were higher than (3.37 ±1.13)mU/L of C group (t=14.650,8.232,15.522,6.037,all P<0.05).FT4 level of A group was lower than that of B group,TSH level of A group was higher than B group (t=7.185,11.097,P<0.05).TC, LDL-C levels of A group,B group were (5.96 ±1.01)mmol/L,(4.15 ±1.69)mmol/L,(5.64 ±0.94)mmol/L, (3.64 ±1.17)mmol/L respectively,which were higher than those of C group [(5.17 ±1.12)mmol/L,(3.20 ± 1.06)mmol/L](t=4.400,2.754,3.992,2.374,all P<0.05).TC,LDL-C levels of A group were higher than B group (t=1.990,2.139,all P<0.05).CIMT,CRP levels of A group,B group were (0.90 ±0.23)mm,(6.20 ± 1.31)mg/L,(0.79 ±0.17)mm,(4.55 ±1.05)mg/L respectively,which were higher than those of C group [(0.68 ±0.11)mm,(3.12 ±0.88)mg/L](t=7.229,4.599,16.364,8.878,all P<0.05).CIMT,CRP levels of A group were higher than B group (t=3.312,8.453,all P<0.05 ).CIMT and TSH,LDL -C,CRP levels were positively correlated (r=0.789,0.647,0.583,all P<0.05),CIMT and FT4 levels were negatively correlated (r=-0.636,P<0.05 ).Conclusion Hypothyroidism,subclinical hypothyroidism are associated with atherosclerosis in elderly population,and elderly patients with hypothyroidism,subclinical hypothyroidism are at high risk of atherosclerosis.

OBJECTIVE:To establish a RP-HPLC method for the determination of the content of emodin in Compound shouwu mixture.METHODS:The determination was performed on Diamonsil C_(18) column(150 mm?4.6 mm,5?m) with the mobile phase consisted of methanol- 0.1%phosphoric acid(68:32).The detective wavelength was set at 220 nm.RESULTS: The linear range of emodin was 7.8～78.0?g?mL~(-1)(r= 0.999 8) with an average recovery rate of 98.25%(RSD = 1.09%,n=9).CONCLUSION:The method is accurate,sensitive and reproducible,and it is applicable for the quality control of the Compound shouwu mixture.

OBJECTIVE:To compare pharmacokinetic parameters of the common tablets and sustained-release melatonin tablets in beagle dogs in vivo.METHODS:The blood concentration of melatonin of6Beagle dogs was determined by HPLC and the pharmacokinetic parameters were calculated with3p97software after administered random crossover with single dose of sustained-release melatonin tablets6mg or the common tablets3mg.RESULTS:The concentration-time curves of both the common tablets and sustained-release melatonin tablets were in conformity with two-compartment model,C max of common tablets and sustained-release tablets were(11.27?3.77)ng/ml and(8.31?5.11)ng/ml respectively,t max of which were(0.50?0.18)h and(1.00?0.37)h respectively,t 1/2ke of which were(1.21?0.52)h and(3.27?0.89)h,AUC 0～t of which were(25.23?7.71)(ng?h)/ml and(38.03?16.45)(ng?h)/ml respectively.CONCLUSION:Compared with the common tablets,the sustained-release tablets showed slower absorption,longer peak time,lower peak concentration,slower elimination and longer duration.

Objective To investigate the variation of myocardial glycometabolism and the release of cardiac creatine kinase and cardiac troponin I on isolated rat heart after stimulated with hyperinsulinism.Methods Thirty Sprague-Dawley rats were random divided into five groups,and their hearts were taken out to be mounted onto a Langendorff perfusion apprartus to perfuse with krebs-Henseleit buffer contained diversity concentration of insulin as 0,10,20,30,50 U/L.The coronary effluent was collected on time,and the concentration of glum was determined to evaluate the glycometabolism of the rat heart,meanwhile the release of cardiac creutine kinase(CK)and cardiac troponin I were detected to evaluate the state of myocardial damage.Results Compared with control group,the rat hearts of the hyperinsulinism group had a significantly depression of glucose uptake and oxidation,and the level of CK and cTn-I in the coronary effluent was increased.Conclusion After the isolated rat hearts were stimulated with hyperinsulinism,the glucose uptake of cadiocyte were suppressed,myocardial insulin resistance appeared,and the rat myocardial got damaged.

Objective To investigate the process of apoptosis in lungs and liver induced by crushing hindlimbs of rat, and study the mechanismof crush injury. Methods The rat experimental model of hindlimbs crush injury was established. The cell apoptosis in lungs and liver was detected by TUNEL assay, and the expression of Bax, Bcl-2 and caspase-3 apoptin was examined by immunohistochemistry. Results C ompared with the control group, the partial muscle injury of rat’s hindlimbs was more serious with more apoptosis observed in lungs and liver (P<0.05). The expression of Bax was up-regulated and Bcl-2 was down-regulated, whereas caspase-3 expression was activated (P<0.05). Conclusion The cell apoptosis has increased significantly in lungs and liver after crush injury of hindlimbs in rat. The corre-lation factor released during tissue injury may mediate apoptosis process.