1.Quality Standard for Mongolia Drug Liangxue Shiwei San
Rina SHA ; Chunlong HE ; Huanyun WANG ; Shumin LI
China Pharmacist 2015;(5):756-758
Objective:To establish the identification method for Aucklandia Lappa and Picrorhiza kurrooa Royle ex Benth and the content determination of hydroxysaffor yellow A in Liangxue Shiwei San. Methods:The identification was carried out by TLC. The con-tent of hydroxysaffor yellow A was determined by HPLC. The column was Kormasil C18 (250 mm × 4. 6 mm, 5 μm) and the flow rate was 1. 0 ml·min-1. The mobile phase was methanol-0. 5% acetic acid (30∶70). The detection wavelength was 403 nm and the col-umn temperature was 25℃,and the sample size was 10 μl. Results:The TLC spots were clear with high resolution without interference from the negative sample. A good linearity of hydroxysaffor yellow A was within the range of 1.212-48.480μg·ml-1(r=0.999 8). The average recovery was 98. 13%(RSD=1. 6%,n=6). Conclusion:The established TLC and HPLC methods are simple and accu-rate with good reproducibility, which can be used in the quality control of Liangxue Shiwei San.
2. Effects of zinc ions on biological functions of human umbilical vein endothelial cells
Pang BAO ; Huanyun LIU ; Yuqing WANG ; Yajun TAN ; Lufeng LI ; Chunxin XU ; Lan HUANG ; Xiaohui ZHAO
Chinese Journal of Cardiology 2018;46(5):390-395
Objective:
To evaluate the effect of zinc ions on human umbilical vein endothelial cells biological functions.
Methods:
The primary human umbilical vein endothelial cells were cultured with the ECM medium, and cells were divided into 8 groups: the control group(routine culture,
3.Preliminary study on 99Tc m-3PRGD 2 imaging to verify the anti-angiogenesis mechanism and efficacy of Mongolian medicine Sendeng-4 decoction for rheumatoid arthritis
Hong QU ; Yu WU ; Guojian ZHANG ; Xiangcheng WANG ; Cheng WANG ; Huanyun WANG ; Kaixiu ZHANG ; Wenrui WANG ; Xuemei WANG
Chinese Journal of Nuclear Medicine and Molecular Imaging 2022;42(5):289-293
Objective:To explore the therapeutic mechanism of Mongolian medicine Sendeng-4 decoction for rheumatoid arthritis by 99Tc m-hydrazinonicotinamide-(polyethylene glycol) 4-E[(polyethylene glycol) 4-c((Arg-Gly-Asp)fk)] 2 (3PRGD 2) imaging. Methods:A total of 200 female SD rats (age: 6-7 weeks) were divided into collagen-induced arthritis (CIA) group ( n=176) and blank control group ( n=24). Rats in the CIA group were divided into Sendeng-4 decoction treatment group ( n=24), etanercept treatment group ( n=24), and negative control group ( n=24) by simple random sampling method. 99Tc m-3PRGD 2 SPECT/CT imaging was performed before and after modeling and treatment. The differences of target/non-target (T/NT) ratio and serological, pathological, and immunohistochemical results among groups were compared by one-way analysis of variance or Kruskal-Wallis rank sum test. The correlation was analyzed by Pearson correlation or Spearman correlation analysis. Results:There were 95 (95/176) CIA models successfully established. The T/NT ratios of Sendeng-4 decoction treatment group and etanercept treatment group were lower than that of negative control group (0.260± 0.094, 0.238±0.099, 0.766±0.144 ; F=163.00, P<0.001), while there was no significant difference between the two drug treatment groups ( P>0.05). After drug treatment, serum levels of vascular endothelial growth factor (VEGF), tumor necrosis factor-α (TNF-α) and α vβ 3 were significantly lower than those of negative control group ( F values: 49.43-92.36, all P<0.001), pathological score was also lower than that of negative control group ( H=34.25, P<0.001), and levels of immunohistochemical makers (VEGF, TNF-α, α vβ 3, CD31, CD34) were also lower than those of negative control group ( H values: 13.51-26.84, all P<0.001), while there were no significant differences between the two drug treatment groups (all P>0.05). The T/NT ratios were positively correlated with above indictors in Sendeng-4 decoction treatment group ( r values: 0.56-0.59, rs values: 0.49-0.69), etanercept treatment group ( r values: 0.50-0.55, rs values: 0.46-0.70) and negative control group ( r values: 0.55-0.80, rs values: 0.58-0.86, P<0.001 or P<0.05). Conclusion:Verified by 99Tc m-3PRGD 2 SPECT/CT imaging and molecular pathology, Mongolian medicine Sendeng-4 decoction can inhibit neovascularization by down-regulating vascular factors such as VEGF, resulting in delaying the progression of the disease and improving clinical symptoms.
4.Content determination of 11 components in Terminalia chebula from different origins and comprehensive evaluation of their chemometrics
Xin JIU ; Jun LI ; Huiwen ZHANG ; Yunxia BAI ; Hong LIU ; Huanyun WANG
China Pharmacy 2022;33(3):299-307
OBJECTIVE To establi sh the method for the con tent determination of 11 components in Terminalia chebula from different origins ,and to provide reference for their quality evaluation and superior provenance screening. METHODS Taking 16 batches of T. chebula from different origins as test samples ,high performance liquid chromatography tandem triple quadrupole mass spectrometry was established to determine the contents of 11 components,such as vitexin ,gallic acid ,methyl gallate ,ethyl gallate,ellagic acid ,corilagin,shikimic acid ,ferulic acid ,luteolin,quercetin and rutin. The determination was performed on Shim-pack GIST-HP C 18 column with mobile phase consisted of 0.1% formic acid solution-methanol at the flow rate of 0.25 mL/ min(gradient elution ). The sample size was 3 μL,and the column temperature was 35 ℃. Electrospray ionization source was used in positive and negative ion mode ,with multiple reaction monitoring. The atomized gas flow rate was 3 L/min,the heating gas flow rate was 10 L/min,the interface temperature was 300 ℃,the desolvent temperature was 526 ℃,and the heating block temperature was 400 ℃ . Grey correlation analysis (GRA)and technique for order preference by similarity to ideal solution (TOPSIS)methods were used to compare ,analyze and comprehensively evaluate T. chebula from different origins. RESULTS The results of content determination methodology met the relevant requirements. The contents of 11 components in 16 batches of T. chebula were 7.27-106.38,5 370.24-31 010.43,21.42-1 097.50,4.26-111.09,17 940.42-38 490.18,6 247.26-40 182.18,12 125.94- 209 519.96,2.71-9.04,0.24-44.12,1.49-9.17 and 25.35-126.51 μg/g,respectively. The results of GRA and TOPSIS analysis showed that the comprehensive qualities of sample H 12(from Yunnan ),H11(from Guangxi ),H5(from Hunan ),H14(from Guangdong),H13(from Sichuan ),H8(from Guangdong ),H1(from Yunnan )were better. CONCLUSIONS The established method is fast ,sensitive and reliable ,and can be suitable for comprehensive evaluation of the internal quality and superior provenance screening of T. chebula .
5.Study on HPLC fingerprint and chemical pattern recognition of Mongolian medicine Sanzisan
Yanyan LIU ; Huiwen ZHANG ; Yunxia BAI ; Hong LIU ; Huimin XIA ; Xin JIU ; Huanyun WANG
China Pharmacy 2022;33(3):319-325
OBJECTIVE To establish the HPLC fingerprint of Mongolian medicine Sanzisan ,and to evaluate its internal quality by chemical pattern recognition technique comprehensively. METHODS HPLC method was used. Using geniposide as reference,HPLC fingerprints of 15 batches of Sanzisan were drawn with Similarity Evaluation System of TCM Chromatogram Fingerprint(2012 edition). Similarity evaluation and common peaks identification were conducted. Combined with cluster analysis (CA),principal component analysis (PCA),and orthogonal partial least squares-discriminant analysis (OPLS-DA),the quality of 15 batches of Sanzisan was evaluated ,and the differential markers that affected its quality were screened. RESULTS There were 29 common peaks in 15 batches of Sanzisan ,and the similarity was no less than 0.952,indicating that the chemical composition of the 15 batches of Sanzisan had good consistency. A total of 13 common peaks were identified ,which were chebulic acid ,gallic acid,punicalin,punicalagin A ,punicalagin B ,jasminoside B ,caffeic acid ,corilagin,geniposide,chebulagic acid ,1,2,3,4,6- O-galloylglucose,chebulinic acid ,ellagic acid. Both CA and PCA could divide 15 batches of Sanzisan into four categories ,and the classification results were consistent ,indicating that the quality of 15 batches of Sanzisan had certain differences. Fourteen differential markers (chebulic acid ,gallic acid ,ellagic acid ,etc)that lead to the quality difference between batches were screened out by OPLS-DA. CONCLUSIONS Established HPLC fingerprint analysis method is simple and stable. Combined with chemical pattern recognition analysis ,it can be used for the quality control of Sanzisan.
6.Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis
Lijun SUN ; Jun LI ; Qiutong WANG ; Huimin XIA ; Yuewu WANG ; Qian ZHANG ; Huiwen ZHANG ; Huanyun WANG
China Pharmacy 2023;34(24):3006-3011
OBJECTIVE To establish the methods to identify the chemical components of Ixeris chinensis, and determine the contents of 7 components (chlorogenic acid, luteolin, quercetin, rutin, protocatechuic acid, isochlorogenic acid A, luteoloside). METHODS HPLC-Q-Exactive-MS was used to identify the chemical components of I. chinensis. The contents of 7 components in I. chinensis, including chlorogenic acid, were determined by HPLC-MS/MS. RESULTS A total of 45 components were identified in I. chinensis, including 20 organic acids, 13 flavonoids, 4 fatty acids, 4 amino acids, 3 nucleosides, and 1 coumarin. The linear range of chlorogenic acid, luteolin, quercetin, rutin, protocatechuic acid, isochlorogenic acid A and luteoloside were 503.00- 25 150.00, 42.00-2 100.00, 5.05-252.50, 20.05-1 002.50, 25.10-1 255.00, 750.00-37 500.00, 196.00-9 800.00 ng/mL (r≥0.999 2), respectively. RSDs of precision, stability and reproducibility tests were all less than 3.00% (n=6), and average recovery ranged from 96.72% to 105.84% (all RSD<4.00%, n=6). The contents of 7 components in 3 batches of I. chinensis were 1 145.77- 3 261.25, 23.75-97.90, 0.92-2.12, 1.06-23.18, 9.35-21.85, 833.25-1 045.58, 199.56-1 869.78 μg/g, respectively. CONCLUSIONS The established methods for identification and content determination are rapid and simple, and can be used for the identification of chemical components and the content determination of 7 components in I. chinensis.