Objective To evaluate the usage situation and the trend of traditional Chinese medicine injection for activating blood circulation in our hospital. Methods The sales amount and drug use frequency (DDDs) and drug utilization index (DUI) of traditional Chinese medicine injection for activating blood circulation were sorted and analyzed in our hospital from the fourth quarter of 2011 to the third quarter of 2012. Results The total sales amount of traditional Chinese medicine injection for activating blood circulation gradually increased in our hospital during that time. The proportion of varieties with the ratio DUI sum/DDDs sort of closed to 1 (0.75≤order ratio≤1.25) in the three quarters of 2012 accounted for 53.33%, 53.33%, and 66.67%, respectively. This indicated that the usage of traditional Chinese medicine injection for activating blood circulation was more unreasonable, but tended to be reasonable. Conclusion The usage of traditional Chinese medicine injection for activating blood circulation was basically rational in our hospital, and efficacy of clinical pharmacist intervention was obvious. Effective and safe evaluation of traditional Chinese medicine injection can be further strengthened, in order to improve the efficacy and reduce the adverse reaction.

Objective To detect the expression of KL-6 mucin in the tissue and serum of hepatoma in different hepatoma patients,and to investigate the value of KL-6 mucin as a tumor marker in the diagnosis of hepatoma.Methods The expression of KL-6 mucin in the hepatoma tissues of 81 patients with hepatocellular carcinoma(HCC),21 patients with cholangiocarcinoma(CC),12 patients with combined hepatocellular and cholangiocarcinoma(HCC-CC)and 56 patients with metastatic liver cancer(MLC)was detected by immunohistochemical analysis.The expression of KL-6 mucin in the serums of 34 HCC patients,8 CC patients,30 MLC patients and 19 healthy individuals was detected by enzyme-linked immunosorbent assay,and all the data were analyzed by t test.Results Expression of KL-6 mucin was detected in the cholangiocarcinoma tissues in all CCand HCC-CC patients.In several hepatoma cells and partial hepatoma tissues of patients with MLC,the expression of KL-6 mucin was detected.No expression of KL-6 mucin was detected in the hepatocellular carcinoma tissuesand non-cancerous tissues of patients with HCC or HCC-CC.The serum levels of KL-6 mucin expression in CC patients were signifcantly higher than those in healthy individuals,HCC and MLC patients(t=5.58,5.34,4.00.P＜0.01).The difference of the serum levels of KL-6 mucin expression between MLC patients and healthy individuals had statistical significance(t=2.77,P＜0.01).However,no significant difference in serum levels of KL-6 mucin expression was found between HCC patients and healthy individuals and between HCC patients and MLC patients(t=2.03,1.89,P＞0.05).Conclusion The expression of KL-6 inucin in CC patients is significantly higher than in patients with other types of hepatoma in both tissue and serum levels.Thus,KL-6 may be a usetul new tmnor marker for distinguishing CC from other types of hepatoma.

The expression of resistin protein in normal human abdominal, thigh, pregnant women abdominal, non-pregnant women abdominal subcutaneous adipose tissue and placenta and the relationship between obesity, type 2 diabetes mellitus (T2DM), pregnant physiological insulin resistance (IR) and gestational diabetes mellitus (GDM) was investigated. The expression of resistin protein in normal human abdominal, thigh, pregnant women abdominal, non-pregnant women abdominal subcutaneous adipose tissue and placenta was detected by using Western blotting method. Fasting serum glucose concentration was measured by glucose oxidase assay. Serum cholesterol (CHOL), serum triglycerides (TG), serum HDL cholesterol (HDL-C) and serum LDL cholesterol (LDL-C) were determined by full automatic biochemical instrument. Fasting insulin was measured by enzyme immunoassay to calculate insulin resistance index (IRI). Height, weight, systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured to calculate body mass index (BMI) and body fat percentage (BF %). Resistin protein expression in pregnant women placental tissue (67 905 +/- 8441) (arbitrary A values) was much higher than that in subcutaneous adipose tissue in pregnant women abdomen (40 718 +/- 3818, P < 0.01), non-pregnant women abdomen (38 288 +/- 2084, P < 0.01), normal human abdomen (39 421 +/- 6087, P < 0.01) and thigh (14 942 +/- 6706, P < 0.001) respectively. The resistin expression in abdominal subcutaneous adipose tissue showed no significant difference among pregnant, non-pregnant women and normal human, but much higher than that in thigh subcutaneous adipose tissue (P < 0.001). Pearson analysis revealed that resistin protein was correlated with BMI (r = 0.42), fasting insulin concentration (r = 0.38), IRI (r = 0.34), BF % (r = 0.43) and fasting glucose (r = 0.39), but not with blood pressure, CHOL, TG, HDL-C and LDL-C. It was suggested that resistin protein expression in human abdominal subcutaneous adipose tissue was much higher than that in human thigh subcutaneous adipose tissue. Resistin was closely related with central obesity, leading to IR, subsequently obesity and T2DM. Resistin protein expression in placental tissue was much higher than that in subcutaneous adipose tissue in normal human abdomen, pregnant abdomen, non-pregnant women abdomen and thigh. It was indicated that resistin protein could be secreted from human placental tissue. Resistin might be one of the factors that lead to pregnant physiological IR and GDM.

Objective:To investigate the clinicopathological and molecular genetic characteristics of tall cell variant and hobnail variant of papillary thyroid carcinoma (PTC).Methods:Twenty-one cases of tall cell variant (TCV-PTC) of PTC (TCV-PTC) and ten cases of hobnail variant of PTC (HV-PTC), as the highly aggressive group, were collected from Xuanwu Hospital from August 2009 to August 2015. Twenty-two cases of follicular variant and 21 classical PTC cases were included as control. Relevant clinical and pathologic data were obtained, and in some cases, paraffin samples were selected for gene mutation spectrum analysis using second generation sequencing.Results:There were 18 males and 56 females; 57 patients were younger than 55 years of age, and 17 patients were 55 years or older. The mean tumor size was 1.6 cm for the high-aggressive group (TCV-PTC and HV-PTC), 1.1 cm for the follicular subtype, and 1.6 cm for the classical type. There were 54 cases with thyroid capsule invasion, 24 cases with extra-thyroidal invasion, and 45 patients with lymph node metastases. Regional recurrence occurred in 7 cases, no recurrence in 54 cases, and 13 patients were lost to follow-up. The highly aggressive group was more likely to show extra-thyroidal invasion, lymph node metastases and recurrence than those with classical PTC ( P<0.05). Within this cohort, BRAF V600E mutation was detected in 53 cases and TERT promoter mutation in 6 cases. Compared with the single mutation group and no mutation group, BRAF and TERT promoter co-mutation group was more commonly detected in older age, male, larger tumor size and more prone to extra-thyroid invasion ( P<0.05). In addition, among BRAF and TERT co-mutation cases, the highly-aggressive group accounted for the highest proportion (5/6). Conclusions:TCV-PTC and HV-PTC, as highly-aggressive variants of PTC, show more aggressive biologic behavior (more lymph node metastasis, external thyroid invasion and recurrences) than the classical and follicular variants of PTC. Coexisting BRAF and TERT promoter mutations may be associated with invasive biologic behavior.

Objective To observe the effect of Zhongtong'an Capsules combined with rivaroxa-ban tablets on deep venous thrombosis(DVT)of lower extremities after total knee arthroplasty.Methods A total of 82 patients were randomly divided into control group and experimental group,with 41 cases in each group.The control group was treated with rivaroxaban tablets after surgery,while the experimental group was treated with oral administration of Zhongtong'an Capsules on the ba-sis of the control group.The main outcome indicators(occurrence of DVT of lower extremities),sec-ondary outcome indicators[circumference of lower extremities,blood flow velocity of femoral deep vein,whole blood high shear viscosity,whole blood low shear viscosity,hematocrit,plasma viscosity index,serum prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen(FIB),and plasma D-dimer(D-D)],and therapeutic effects[the Visual Analogue Scale(VAS)score for pain,score of American Hospital for Special Surgery(HSS)forknee function]were compared be-tween the two groups.Results After 14 days of treatment,the incidence of DVT of lower extremities in the experimental group was 9.76％(4/41),which was significantly lower than 31.71％(13/41)in the control group(x2=6.011,P＜0.05).Compared with the control group,the experimental group had faster venous blood flow velocity and reduced circumference of lower extremities after 7 and 14 days of treatment(P＜0.05).After 7 and 14 days of treatment,all the hemorheological-related indicators in the experimental group were significantly lower than those in the control group(P＜0.05).After 7 and 14 days of treatment,the PT and APTT in the experimental group were significantly longer than those in the control group,while the FIB and D-D levels were significantly lower than those in the control group(P＜0.05).After 7 and 14 days of treatment,the VAS scores decreased and the HSS scores increased significantly in both groups,and the VAS scores in the experimental group were significantly lower than those in the control group,while the HSS scores were significantly high-er than those in the control group(P＜0.05).Conclusion Zhongtong'an Capsules combined with rivaroxaban tablets can effectively improve the hypercoagulable state of patients after knee arthroplas-ty and reduce the formation of postoperative DVT.

Objective To observe the effect of Zhongtong'an Capsules combined with rivaroxa-ban tablets on deep venous thrombosis(DVT)of lower extremities after total knee arthroplasty.Methods A total of 82 patients were randomly divided into control group and experimental group,with 41 cases in each group.The control group was treated with rivaroxaban tablets after surgery,while the experimental group was treated with oral administration of Zhongtong'an Capsules on the ba-sis of the control group.The main outcome indicators(occurrence of DVT of lower extremities),sec-ondary outcome indicators[circumference of lower extremities,blood flow velocity of femoral deep vein,whole blood high shear viscosity,whole blood low shear viscosity,hematocrit,plasma viscosity index,serum prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen(FIB),and plasma D-dimer(D-D)],and therapeutic effects[the Visual Analogue Scale(VAS)score for pain,score of American Hospital for Special Surgery(HSS)forknee function]were compared be-tween the two groups.Results After 14 days of treatment,the incidence of DVT of lower extremities in the experimental group was 9.76％(4/41),which was significantly lower than 31.71％(13/41)in the control group(x2=6.011,P＜0.05).Compared with the control group,the experimental group had faster venous blood flow velocity and reduced circumference of lower extremities after 7 and 14 days of treatment(P＜0.05).After 7 and 14 days of treatment,all the hemorheological-related indicators in the experimental group were significantly lower than those in the control group(P＜0.05).After 7 and 14 days of treatment,the PT and APTT in the experimental group were significantly longer than those in the control group,while the FIB and D-D levels were significantly lower than those in the control group(P＜0.05).After 7 and 14 days of treatment,the VAS scores decreased and the HSS scores increased significantly in both groups,and the VAS scores in the experimental group were significantly lower than those in the control group,while the HSS scores were significantly high-er than those in the control group(P＜0.05).Conclusion Zhongtong'an Capsules combined with rivaroxaban tablets can effectively improve the hypercoagulable state of patients after knee arthroplas-ty and reduce the formation of postoperative DVT.

The expression of resistin protein in normal human abdominal, thigh, pregnant women abdominal, non-pregnant women abdominal subcutaneous adipose tissue and placenta and the relationship between obesity, type 2 diabetes mellitus (T2DM), pregnant physiological insulin resistance (IR) and gestational diabetes mellitus (GDM) was investigated. The expression of resistin protein in normal human abdominal, thigh, pregnant women abdominal, non-pregnant women abdominal subcutaneous adipose tissue and placenta was detected by using Western blotting method.Fasting serum glucose concentration was measured by glucose oxidase assay. Serum cholesterol (CHOL), serum triglycerides (TG), serum HDL cholesterol (HDL-C) and serum LDL cholesterol (LDL-C) were determined by full automatic biochemical instrument. Fasting insulin was measured by enzyme immunoassay to calculate insulin resistance index (IRI). Height, weight, systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured to calculate body mass index (BMI) and body fat percentage (BF %). Resistin protein expression in pregnant women placental tissue (67 905±8441) (arbitrary A values) was much higher than that in subcutaneous adipose tissue in pregnant women abdomen (40 718 ± 3818, P ＜ 0.01), non-pregnant women abdomen (38 288±2084, P＜0.01), normal human abdomen (39 421±6087, P＜0.01)and thigh (14 942 ±6706, P＜0. 001) respectively. The resistin expression in abdominal subcutaneous adipose tissue showed no significant difference among pregnant, non-pregnant women and normal human, but much higher than that in thigh subcutaneous adipose tissue (P＜0. 001). Pearson analysis revealed that resistin protein was correlated with BMI (r=0.42), fasting insulin concentration (r=0.38),IRI (r=0. 34), BF % (r=0.43) and fasting glucose (r=0. 39), but not with blood pressure,CHOL, TG, HDL-C and LDL-C. It was suggested that resistin protein expression in human abdominal subcutaneous adipose tissue was much higher than that in human thigh subcutaneous adipose tissue. Resistin was closely related with central obesity, leading to IR, subsequently obesity and T2DM. Resistin protein expression in placental tissue was much higher than that in subcutaneous adipose tissue in normal human abdomen, pregnant abdomen, non-pregnant women abdomen and thigh. It was indicated that resistin protein could be secreted from human placental tissue. Resistin might be one of the factors that lead to pregnant physiological IR and GDM.

OBJECTIVETo investigate the effect of bone marrow mesenchymal stem cells (BMSCs) on secretion of inflammatory cytokines in macrophages stimulated by lipopolysacharide (LPS).

METHODSRat BMSCs and macrophages were isolated, cultured, and identified. The BMSCs and macrophages, cultured alone or in co-culture, were treated with LPS or PBS or without treatment and tested for interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) concentrations in the supernatants at 1, 3, 5, 7, 12, and 24 h after the treatment using enzyme-linked immunosorbent assay.

RESULTSExposure to LPS caused significantly increased IL-10 and TNF-α concentrations in the supernatant of cultured macrophages but not in BMSC culture. Macrophages co-cultured with BMSCs showed significantly lowered IL-10 and TNF-α secretions in response to LPS exposure as compared with the macrophages cultured alone.

CONCLUSIONBMSCs can reduce LPS-induced secretion of inflammatory cytokines by the macrophages to ameliorate inflammatory reactions.

Animals ; Cells, Cultured ; Coculture Techniques ; Enzyme-Linked Immunosorbent Assay ; Inflammation ; Interleukin-10 ; secretion ; Lipopolysaccharides ; Macrophages ; secretion ; Mesenchymal Stromal Cells ; cytology ; Rats ; Tumor Necrosis Factor-alpha ; secretion