Objective:To investigate the relationship between uterine artery blood flow parameters detected by ultrasound examination during early pregnancy and pregnancy outcomes in pregnant women who had a history of recurrent spontaneous abortion.Methods:A total of 100 pregnant women with a history of recurrent spontaneous abortion who were at 6-10 weeks of gestation and received treatment in Zhuji Maternal and Child Health Hospital from June 2020 to June 2022 were included in the study group. Using a case-control study method, 100 normal pregnant women who were at 6-10 weeks of gestation who concurrently underwent physical examination were selected and included in the control group. Both groups of pregnant women were tested for uterine artery blood flow parameters using color Doppler ultrasound diagnostic equipment, including peak systolic/diastolic velocity (S/D), resistance index (RI), and pulsatility index (PI). The occurrence of early miscarriage was monitored in both groups. The value of uterine artery blood flow parameters in the prediction of adverse pregnancy outcomes and the correlation between uterine artery blood flow parameters and adverse pregnancy outcomes were analyzed. Multivariate logistic regression was performed to analyze the relationship between uterine artery blood flow parameters and adverse pregnancy outcomes.Results:S/D, RI, and PI in the study group were (3.87 ± 0.43), (0.93 ± 0.16), and (1.73 ± 0.41), respectively, which were significantly higher than (2.54 ± 0.37), (0.64 ± 0.14), and (1.24 ± 0.35) in the control group ( t = 23.44, 13.64, 9.09, all P < 0.05). The early abortion rate in the study group was 57.0% (57/100), which was significantly higher than 2.0% (2/100) in the control group ( χ2 = 72.73, P < 0.05). The receiver operating characteristic curve analysis showed that S/D sensitivity and specificity in the prediction of adverse pregnancy outcomes were 79.1% and 70.2%, respectively, RI sensitivity and specificity were 88.4% and 84.2%, respectively, and PI sensitivity and specificity were 65.1% and 75.4%, respectively. Pearson analysis results showed that S/D, RI, and PI were positively linearly correlated with adverse pregnancy outcomes ( r = 0.587, 0.648, 0.613, all P < 0.05). Conclusion:Uterine artery blood flow parameters S/D, RI, and PI are abnormally high in pregnant women with a history of recurrent spontaneous abortion in the early stage of pregnancy. These parameters are closely associated with adverse pregnancy outcomes and are the independent risk factors of adverse pregnancy outcomes.

Objective:To explore the expression and clinical significance of miR-143 in papillary thyroid cancer (PTC) .Methods:Tumor samples and adjacent tissues from 52 patients with PTC were obtained from Jan. 1st, 2018 to Mar. 31st, 2018 in Thyroid Surgery Department of the Affiliated Yantai Yuhuangding Hospital of Qingdao University. Quantitative reverse-transcriptase PCR (RT-qPCR) was used to measure the expression of miR-143 in those subjects. In addition, the relationship between the expression levels of miR-143 and the clinicopathological characteristics was analyzed.Results:RT-qPCR indicated that the expression of miR-143 was down-regulated in PTC, which was significantly lower than that in adjacent tissues ( t=-21.39, 95% CI: 18.20-15.07, P<0.001) . Low expression of miR-143 was related to the number of lymph node metastasis ≥3 in central compartment ( t=10.13, P=0.012) and lateral neck lymph node metastasis ( t=-4.67, P<0.001) . Conclusion:Downregulation of miR-143 in PTC is linked to the metastasis of PTC and may be a potential target for therapeutic intervention.

Objective:To explore the clinical significance of recurrent laryngeal nerve inlet zone(RLNIZ) lymph node metastasis in papillary thyroid cancer(PTC).Methods:The clinical data of the clinicopathologic characteristics of 738 cases with papillary thyroid cancer at our centers from Jul 2017 to Jun 2018 was retrospectively reviewed. 108 cases with RLNIZ lymph node dissection for pathological examination were included. The relationship between metastasis of RLNIZ lymph node and clinicopathologic characteristics was analyzed.Results:RLNIZ lymph node was detected in 12.3%(91/738)cases, the mean lymph node number in RLNIZ was 1.5±0.7, and 30.8%(28/91) cases suffered RLNIZ lymph node metastasis. RLNIZ lymph node metastasis(LNM) is associated with tumor size( P=0.028), capsular invasion( P=0.019), No. of central compartment LNM( P<0.001) and lateral neck LNM( P<0.001). No. of central compartment LNM was found to be the independent risk factor of RLNIZ lymph node metastasis. The incidence of dysphagia and inferior parathyroid damage was 0.9%(1/108)respectively. Conclusions:RLNIZ lymph node metastasis is common among PTC patients , therefore, RLNIZ lymph node should be routinely removed especially in patients with tumor size over 1cm、suspected capsular invasion and lateral neck lymph node metastasis confirmed by preoperative imaging examination.

Objective: To explore the clinical significance of metastasis of lymph nodes between sternocleidomastoid and sternohyoid muscle (LNSS) in papillary thyroid cancer (PTC). Methods: A total of 175 patients with PTC who underwent thyroidectomy with LNSS dissection were retrospectively analyzed. Univariate and multivariate Logistic regression analyses were used to determine the independent risk factors for LNSS metastasis in PTC. Results: The rate of detectable LNSS was 70.9% (124/175) and metastasis rate was 7.4% (13/175). Of 13 cases with LNSS metastasis, 10 with the coexistence of cervical lymph node metastasis. Univariate Logistic regression analysis showed that multiple focal cancer, tumor located in the lower pole of thyroid, belt-shaped muscle invasion, lateral cervical lymph node metastasis, cN+ , the number of cervical lymph nodes with metastasis and the number of lymph nodes with metastasis in level Ⅳwere the risk factors for LNSS metastasis (P<0.05). Multivariate Logistic regression analysis suggested that tumor located in the lower pole of thyroid and the number of cervical lymph nodes with metastasis >6 were the independent risk factors for LNSS metastasis (P<0.05). Given the number of cervical lymph nodes with metastasis as a predictor for the LNSS metastasis, the sensitivity was 92.3%, the specificity was 66.7% and the accuracy rate was 68.6%. Conclusions LNSS metastasis is commom in PTC, with a metastasis rate of 7.4%. PTC in the lower pole of thyroid and the number of cervical lymph nodes with metastasis > 6 are independent risks for LNSS metastasis.