Hepatocyte transplantation is an effective method for the end stage liver diseases. This procedure includes hepatoeyte isolation, culture, cryopreservation and so on. The cryopreservation is an important aspect for hepatocyte transplantation, which determines the viability after the resuscitation. The hepatocyte cryopreservation will be summarized in this passage.

Objective To explore the therapeutic effect of budesonide combined with salbutamol on serum interleukin (IL-6) and tumor necrosis factorα (TNF-α) in treatment of bronchial pneumonia. Methods A total of 110 cases of bronchial pneumonia patients were randomly divided into the study group and the normal group, with 55 cases in each group, the two groups were given oxygen, anti-inflammatory and symptomatic treatment, the study group received budesonide combined with salbutamol treatment, the curative effect and changes of serum IL-6, TNF-alpha levels were compared between two groups. Results The disappeared time of cough, fever, shortness of breath,rhonchi and moist rales, hospitalization time in study group were significantly shorter than the conventional group, the difference was statistically significant (P<0.05). After 5 days' treatment, the serum IL-6, TNF-α in study group were lower than normal group(P<0.05), the PaO2, SaO2 in study group were higher than normal group (P<0.05). The efficacy in study group was higher than normal group (P<0.05). Conclusion Budesonide combined with salbutamol in treatment of bronchial pneumonia patients could improve clinical symptoms and anti-inflammatory effect, so as to shorten the treatment time and improve the clinical effect.

Cancer and stroke are the major diseases causing serious harm to human health.Studies show that as compared with normal people,patients with cancer are more prone to stroke.We reviewed the research progress on cancer patients with stroke within the past decade,including the common cancer type which causes stroke,the stroke pathogenesis of cancer patients,clinical characteristics,treatment and prognosis of cancer patients with comorbid stroke,in order to provide the evidence for the prevention and treatment of stroke in cancer patients.

As one important part of external conditions,a harmonious social environment is necessary precondition for human health.This article inquires the function of a harmonious social environment to human health from internal demand,basic prerequisite and post-disaster social environmental support of human health.

Objective:To analyze clinical and pathological features of Reed nevi (pigmented spindle cell nevi) .Methods:The clinical and pathological data were collected from 26 patients with Reed nevi, who visited Department of Dermatology of Xijing Hospital from January 2015 to June 2019, and analyzed retrospectively.Results:Among the 26 patients, 15 were males, and 11 were females. Their average age of onset was 17.35 years, and median age of onset was 13.85 years. Reed nevi occurred predominantly on the lower limbs (12 cases) , followed by the face (6 cases) , upper limbs (5 cases) and trunk (3 cases) . All the skin lesions were black in color, and 7 cases presented with maculae, 19 with flat papules, 22 with quasi-circular skin lesions, and 4 with irregular skin lesions. The diameter of skin lesions ranged from 2 to 10 mm, and 23 cases had skin lesions with a diameter of ≤ 5 mm. As histopathological examination showed, pathological subtypes included junctional nevus (15 cases) and compound nevus (11 cases) ; melanocytes were spindle-shaped with obvious cytochromes in 26 cases; nevus cell nests merged with the surrounding epidermis in 4 cases, and obvious fissures were formed between nevus cell nests and the surrounding epidermis in 22 cases; no obvious cell atypia or mitotic figure was observed in any of the 26 cases, and Kamino bodies were observed in 6 cases. All the skin lesions were resected by surgery, and no recurrence was observed during the follow-up of 1 - 5 years.Conclusion:Reed nevi can manifest as irregular lesions, pagetoid distribution of spindle cells of various sizes can be observed histopathologically, and they need to be differentiated from melanoma.

Objective To establish a detection method of Salmonella in laboratory animals based on a high-throughput sequencing technology, and to apply it in detection of Salmonella in laboratory animals.Methods DNA samples were extracted from mouse feces.Universal primers for 16S rDNA, 23S rDNA, 16S-23S rDNA, 23S-5S rDNA region, gyrB preferred area were designed, respectively.Each primer was tested and analyzed to determine the best amplification conditions and build a database.Forty-two samples of Salmonella were assayed by Illumina high-throughput sequencing technology and evaluated the specificity and stability of this method.Results The species preferred region of Salmonella was gyrB gene region.The primers for gyrB gene were FP5 ’-AACCACCGCAATCAGACCTT3‘ and FP5 ’-AGCCACGAAACCTTCACYA-3’.The primers were optimized and determined, through a high-throughput sequencing, and the sequence analysis detected very small amount of Salmonella in the 42 samples, indicating that this detection method is stable, highly sensitive, and the limit of detection reached to 0-102 CFU.Conclusions We have established a complete detection system for detection of Salmonella in laboratory animals based on a high-throughput sequencing technology, This system can detect trace amounts of Salmonella in laboratory animals, and this detection method is stable and highly sensitive, which can be also used in detection of other kinds of pathogenic microorganism in laboratory animals.

Objective To investigate the relationship between the expression of trannsient receptor potential vanilloid 1 (TRPV1) and the severity of airway remodeling in elderly patients with chronic obstructive pulmonary disease(COPD).Methods According to airflow obstruction severity,totally 100 cases of elderly patients with COPD were divided into chronic obstructive pulmonary disease Global Initiative(gold) grade 1 in 23 cases,24 cases of grade GOLD2,GOLD3 27 cases,GOLD4 26 cases,respectively.The TRPV1 concentrations in induced sputum supernatant and serum from each level of elderly patients with COPD as well as in 50 cases of healthy old people were analyzed.Results TRPV1 concentrations in serum and induced sputum in the COPD group was significantly increased compared with the healthy elderly group[(9.94±2.91)μg/L vs.(3.68±0.46)μg/L,(3.29± 1.32)μg/L vs.(0.70 ± 0.30)μg/L] (P ＜ 0.01).The serum and induced sputum TRPV1 concentrations in the mutual pairwise comparison between the elderly COPD patients with all levels had statistical difference (P ＜ 0.01).Conclusion The expression of TRPV1 protein become increased with the severity of airway remodeling in elderly patients with chronic obstructive pulmonary disease.

This study was aimed to analyze effects on quality of life (QOL) for stable chronic obstructive pulmonary disease (COPD) by Shu-Fei Paste (SFP) with different sticking time, in order to determine the optimal sticking time. A multicenter, randomized, controlled clinical trial was designed. A total of 164 stable COPD cases were randomly divided into Group A and Group B. There were 82 cases in Group A (sticking time for 6-8 h), and 82 cases in Group B (sticking time for 8-12 h). The paste was given 10 days before the summer “dog days”, on the 1st, 10th, and 20th day during the summer “dog days”, and 10 days after the summer “dog days”. At the end of the study, 18 cases were fallen off or removed. The 146 cases (71 cases in Group A and 75 cases in Group B), which met the require-ments of the study, were analyzed. The results showed that both groups can improve QOL in patients with stable COPD after treatment. However, there were no statistical differences. There were no statistical differences between the sticking time for 6-8 h and sticking time for 8-12 h. However, the sticking time for 6-8 h with low incidence of severe bullous and better safety. Therefore, the best choice of sticking time was 6-8 h. It was concluded that there was no statistical differences on the treatment between the sticking time for 6-8 h and 8-12 h. However, the sticking time for 8-12 h had high rate of severe bullous and low safety. The best sticking time of SFP was 6-8 h.

This study was aimed to analyze the therapeutic effect of Shu-lung post with different stimula-tion intensities on chronic obstructive pulmonary disease (COPD), in order to determine the best stimulus intensity. A multicenter, randomized, controlled clinical trial was designed to take COPD patients at stable stage as study sub-jects. Patients were randomly divided into two groups, which were the foaming group and non-foaming group. The application was given 10 days before the summer dog days, on the 1st, 10th and 20th day within the dog days, and 10 days after the dog days. The results showed that a total of 164 cases were enrolled in the study, with 18 drop-out cases, and 146 cases fitted in the program. There was statistical difference on acute exacerbation frequency
after treatment between the foaming group and non-foaming group (P < 0.05). There were statistical differences on clinical symptoms and signs of total score (P< 0.01) during the 8-month follow-up. It was concluded that Shu-lung post with different stimulation intensities were positively correlation with clinical efficacy.

Objective To observe the effects of different levels manganese (Mn) on spatial learning and memory in neonate rats.Methods Neonate rats were distributed to control (normal saline) and MnCl210,20,30mg/kg groups randomly.Each groups included 10 litters in a cage with a dam.Neonate rats were intraperitoneal injection exposed to MnCl2 over PND 1-21.All groups were evaluated behavioral performance using open field and Morris water maze.Blood and hippocampus Mn levels were determined using ICP-MS.Results 1) For each group,blood Mn were (35.58 ± 13.77) μg/L,(80.00 ± 12.98) μg/L,(238.51 ± 31.43) μg/L,(348.47 ±34.07) μg/L and hippocampus Mn were (576.82 ± 79.78) μg/g,(798.33 ± 40.60) μg/g,(1017.23 ± 117.23)μg/g,(1278.76 ± 281.48) μg/g respectively.Blood and hippocampus Mn concentrations in Mn-exposed groups were significant increased compared to control (P ＜ 0.01),and there was a positive correlation in blood Mn and hippocampus Mn(OR =0.91,95％ CI=0.81-0.96,P＜ 0.01).2) Therewere no significant differences on travelled distance in open field among all groups,which meant that Mn exposure had no effect on their locomotion.3) In the hidden platform trials of the Morris water maze test,only on 3rd day,Mn-expose groups spent more time to find the platform compared to the control(P ＜ 0.01).The average escape latency were(21.77 ± 7.10)s,(33.78 ± 9.95)s,(37.17 ± 13.68) s,(41.92 ± 16.74) s respectively.Though the latency were increased with the Mn exposure levels increasing among the Mn-expose groups,no statistically significant differences were observed.There were no statistically effects on latency to find the platform of all groups in other training days.The result in probe trails showed that there were no statistically effects on swimming velocity,the number of crossing over the former platform and the time spent in the targeted quadrant.Conclusion Mn exposure exerts effects on the learning,but no doseeffect relationship.There are no effects on memory of neonate rats of Mn exposure.