1.Expression and function of long intergenic non-protein coding RNA-regulator of reprogramming in high-grade ovarian serous cancer
Huanhuan JIANG ; Yanhui LOU ; Xiangyu WANG ; Yi HAN ; Zhumei CUI
Chinese Journal of Obstetrics and Gynecology 2016;51(12):921-927
Objective To investigate the expression of long intergenic non-protein coding RNA-regulator of reprogramming (Linc-ROR) in high-grade ovarian serous cancer, and explore the relationship between Linc-ROR expression and biological function of high-grade ovarian serous cancer. Methods A total of 34 high-grade ovarian serous cancer tissue samples and 19 normal fallopian tube tissue samples were collected between June 2014 and February 2016. Real-time reverse transcription (RT)-PCR was used to detect the Linc-ROR mRNA expression in different samples. The relationship between Linc-ROR expression level and ovarian cancer International Federation of Gynecology and Obstetrics (FIGO) stage, lymph node metastasis was analyzed. Constructed Linc-ROR small interference RNA (siRNA) and pIRES2-EGFP-Linc-ROR plasmid, then Linc-ROR siRNA and pIRES2-EGFP-Linc-ROR plasmid were respectively transfected into SKOV3 cells. Cell proliferation, migration and invasion ability were assessed by cell counting kit-8 (CCK-8), wound healing assay and transwell invasion assay. Results (1) The expression level of Linc-ROR mRNA was significantly higher in high-grade ovarian serous cancer than normal fallopian tube tissues (4.31± 0.38 vs 1.03 ± 0.21; t=25.842, P<0.01). With the progression of FIGO stages, the expression of Linc-ROR was increased (F=95.702, P<0.01), and it was associated with lymph node metastasis (t=7.397, P<0.01). (2) The results of RT-PCR showed that the expression level of linc-ROR in Linc-ROR-i group was significantly lower than that in Linc-ROR-NC-i group (0.30 ± 0.11 vs 1.02 ± 0.10; t=15.269, P<0.01). The expression level in Linc-ROR-p group was significantly higher than that in Linc-ROR-NC-p group (8.90 ± 0.45 vs 1.03 ± 0.17;t=21.934, P<0.01). The CCK-8 assay showed that when the cells were cultured for 3, 4, 5 and 6 days, the A value in Linc-ROR-i group was significantly lower than that in Linc-ROR-NC-i group (P<0.05). And the A value in Linc-ROR-p group was significantly higher than that in Linc-ROR-NC-p group (P<0.05). Wound healing assay showed that, after 48 hours incubation, migration rate of cells in Linc-ROR-i group was significantly less than that in the Linc-ROR-NC-i group [(52±4)%vs(67±5)%;t=5.720,P<0.01]. The migration of cells in Linc-ROR-p group was significantly greater than that in the Linc-ROR-NC-p group [(84±4)%vs(66±4)%;t=7.330,P<0.01]. Cell transwell invasion assay showed that, after 48 hours of incubation, the number of invasive cells in Linc-ROR-i group was lower than that in Linc-ROR-NC-i group (74 ± 3 vs 104 ± 3; t=15.810,P<0.01). And the number of invasive cells in Linc-ROR-p group was higher than that in Linc-ROR-NC-p group (217 ± 4 vs 108 ± 5; t=38.060, P<0.01). Conclusion Highly expressed Linc-ROR could enhance the proliferation, migration and invasion ability of high-grade ovarian serous cancer cells, which may be one of the important molecules in the occurrence and development, invasion and metastasis of high-grade ovarian serous cancer.
2.The Correlation between the Response Thresholds of ASSR and Pure Tone Thresholds in Young Deaf Children
Dongmei SHI ; Huanhuan YI ; Jing LI ; Fugen HAN
Journal of Audiology and Speech Pathology 2016;24(5):452-454
Objective To investigate the correlation between auditory steady-state response (ASSR)and behavioral audiometry threshold in deaf children.Methods A total of 63 cases (126 ears)of severely and profoundly hearing impaired children from 0.5 years to 6 years were divided to Group A of 0.5~1.0 years,Group B of 1.1~3.0 years and Group C of 3.1~6.0 years old.ASSR and behavioral hearing tests were conducted in the sound field. 0.5~1.0 years old children were tested with the behavior observation Audiometry (BOA),1~2 years old children with visual reinforcement audiometry (VRA),while those of 3 ~6 with play audiometry (PA).Results For the ASSR response thresholds and pure tone hearing thresholds at 0.5,1,2 and 4 kHz,the correlation coefficients were 0.75,0.76,0.76,and 0.83,respectively.There was a significant correlation (P <0.01 ).The hearing thresholds were generally lower than the ASSR response thresholds,and with increasing frequency,the gaps were narrowed between the two.For Group A at each frequency the response thresholds and behavioral audiometry correlations were lower than those of Group B and C with the lowest at 0.5 kHz.Conclusion For the children of 0.5~6 years, ASSR and behavioral audiometric thresholds have good correlations.ASSR can provide information about the behav-ior thresholds for young children and those high risk children and for fitting hearing aids.
3.Recombinant lentiviral vector transfected sheep bone marrow mesenchymal stem cells and osteogenic gene expression changes
Xiangzhen HAN ; Huiyu HE ; Yang HU ; Jiaojiao BA ; Huanhuan WANG ; Xue MI
Chinese Journal of Tissue Engineering Research 2014;(6):821-828
METHODS:Lentiviral vectors carrying bFGF and BMP-2 were constructed to transfect sheep bone marrow mesenchymal stem cells. cells were divided into four groups:bFGF group, BMP-2 group, co-transfection group BACKGROUND:Basic fibroblast growth factor (bFGF) can promote the proliferation of bone marrow stromal cells, and bone morphogenetic protein-2 (BMP-2) has an important significance in the induction of new bone formation.
OBJECTIVE:To analyze the effects of bFGF, BMP-2 and their co-transfection on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells and to compare the relative expressions of col agen I, osteocalcin and osteopontin before and after celltransfection, thereby providing theoretical implications for seed cells in the construction of tissue-engineered bone.
and control group. The RNA was extracted using real-time quantitative PCR to detect mRNA levels of col agen I, osteocalcin, and osteopontin.
RESULTS AND CONCLUSION:Significant difference in non-specific osteogenic gene expressions was found among the four groups (P<0.05). bFGF and BMP-2 showed an interaction (P<0.05). Expressions of col agen I and osteocalcin in the co-tranfection group were higher than those in the other three groups (P<0.05), but osteopontin expression exhibited no difference (P>0.05). In vitro experiments showed that the relative expression of col agen I, osteocalcin and osteopontin were higher in the co-transfection group, indicating the cells from the co-transfection group have strongest osteogenic capacity that are suitable for seed cells for bone tissue engineering.
4.Derivatization of Oligosaccharides and Analysis by Matrix-assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry
Huanhuan HAN ; Yan MA ; Lu WANG ; Wanjun ZHANG ; Junying WEI ; Yangjun ZHANG ; Xiaohong QIAN
Chinese Journal of Analytical Chemistry 2010;38(3):307-312
A derivatization method for the determination of oligosaccharides with high sensitivity by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry(MALDI-TOF-MS) based on a labeling regent 1-(4-Cyanophenyl)-4-piperidinecarbohydrazide(CPH) was proposed. The hydrazones were generated by the reaction of the reducing ends of oligosaccharides and the hydrazide of CPH. The derivatives were directly analyzed by MALDI-TOF-MS. Reaction conditions such as reaction temperature, concentration of acetic acid and excess ratio of CPH regent were optimized. When the oligosaccharides reacted with 100 time excess of CPH at 100 ℃ and in 0.125% acetic acid (V/V), the yield was highest. The results showed the derivatization with CPH increased the sensitivity of MS detection and this method was suitable for the analysis of oligosaccharides′ compositions.
5.Assistant role of manifestations under tracheoscopy in the diagnosis of invasive pulmonary aspergillosis in severe patients
Huanhuan TIAN ; Shasha HAN ; Fangyu NING ; Xiaoli LIU ; Xiao HUANG ; Dong HAO ; Xiaozhi WANG ; Tao WANG
Chinese Critical Care Medicine 2021;33(4):478-482
Objective:To evaluate the assistant role of manifestations under tracheoscopy in the diagnosis of invasive pulmonary aspergillosis (IPA) in severe patients.Methods:A retrospective study was conducted. The patients with suspected IPA admitted to intensive care unit (ICU) of Affiliated Hospital of Binzhou Medical College from January 2015 to December 2019 were enrolled. The diagnosis, clinical diagnosis and suspected diagnosis were made according to the grading criteria of Guidelines for the diagnosis and treatment of invasive fungal infection in severe patients (2007). Those who met the criteria were enrolled in the IPA group, and those who did not meet the criteria or other pathogens were enrolled in the non-IPA group. The general data of the patients were collected, and the changes of tracheal and bronchial mucosa under tracheal microscope before and after treatment were recorded, as well as the results of galactomannan (GM) test and aetiology culture of bronchoalveolar lavage fluid (BALF). The baseline, bronchoscopy and pulmonary CT manifestations and their dynamic changes were compared in each group. Results:A total of 142 patients with suspected IPA were finally enrolled. Among them, 12 were pathologically proven IPA, 77 were probable IPA, 22 were possible IPA, and 31 were undefined IPA. Of the 142 patients, 60 had typical manifestations of mucosal injury under bronchoscopy, including 7 proven IPA patients (58.3%), 52 probable IPA patients (67.5%), and 1 possible IPA patient (4.5%), but none undefined IPA patient. The patients undergoing lung CT scan were 12 proven IPA patients (100%), 73 probable IPA patients (94.8%), and 21 possible IPA patients (95.5%), respectively. Most of the Chest CT showed patchy or strip density increasing and other non-specific manifestations. There were 3 proven IPA patients (25.0%), 7 probable IPA patients (9.0%), and 0 possible IPA patient (0%) who had typical IPA CT manifestations (halo sign and cavity or crescent sign). Among the patients of proven IPA and probable IPA (89 cases), there were a total of 35 cases with endoscopic airway mucosal injury and tracheoscopy reexamination ≥ 3 times. All the 35 patients received anti-aspergillus treatment, among which 16 survived and 19 died. Among the 16 patients who survived, the microscopic appearance of mucosal injury was gradually reduced and the clinical manifestations were gradually improved. Of the 19 patients who died, 16 had deteriorated endoscopic airway mucosal injury.Conclusions:The specific manifestations of severe patients with bronchial mucosal injury are of great significance in the diagnosis of IPA. In the case of severe patients who cannot receive pathological examination or chest CT in time, dynamic observation of the changes of airway mucosal injury is a simple auxiliary method to discover the changes of patients' condition in time, evaluate the effect of antifungal therapy and the prognosis of IPA.
6.Community promotion of superficial needling plus club swing for post-stroke motion impairment of the shoulder joint
Huanhuan NI ; Yaochi WU ; Xiangdong SHI ; Yang LI ; Yiyi ZHANG ; Huiling ZENG ; Li JI ; Dequan HUANG ; Chunshui HUANG ; Chouping HAN
Journal of Acupuncture and Tuina Science 2017;15(2):109-114
Objective: To investigate the community promotion feasibility of superficial needling plus club swing for post-stroke motion impairment of the shoulder joint. Methods:A total of 180 cases (duration <1.5 years) with post-stroke motion impairment of the shoulder joint were recruited from three community health centers in Changning District, 60 from each community. They were randomly allocated into an observation group (n=90) and a control group (n=90). Patients in both groups received standard internal and rehabilitation care. Patients in the observation group received additional superficial needling plus club swing. The visual analogue scale (VAS) was conducted before and 60 d after the treatment to evaluate the severity of shoulder pain. The active movement of the shoulder joint and activities of daily living (ADL) were also observed. Results:There were no between-group statistical differences before the treatment (allP>0.05). After a 60-day treatment, the shoulder pain severity, active range of motion of the shoulder joint and ADL in the observation group were significantly improved than those in the control group (allP<0.01). In addition, no adverse events were reported by participants in the observation group. Conclusion:Superficial needling plus club swing plays a positive role in improving post-stroke motion impairment of the shoulder joint. This safe, reliable and economical therapy has good patient compliance and is suitable for community promotion.
7. Clinical effect of high pressure oxygen and Butylphthalide in the recovery of cerebral metabolism after carbon monoxide poisoning
Qian HAN ; Xintao GUO ; Huanhuan ZHANG ; Rui TIAN ; Xiao GAO ; Zhiqing ZHANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(8):591-593
Objective:
To study the clinical effect of high pressure oxygen and Butylphthalide in the recovery of cerebral metabolism after carbon monoxide poisoning.
Methods:
84 patients treated from May 2014 to May 2016 in our hospital were selected. The subjects were randomly and equally divided into two groups. The control group adopted the conventional therapy and high pressure oxygen; on the basis, the observation group also took Butylphthalide. The clinical effect, duration of coma, recovery of consciousness, incidence rate of delayed encephalopathy was observed. After 1m of treatment, the HDS point was evaluated.
Results:
The total effective rate of control group (76.19%, 32/42) was lower than that of observation group (95.24%, 40/42) (
8.Network Meta-Analysis of Effectiveness of First-Line Immunotherapy Treatments for Patients with Brain Metastases from Advanced Non-Small Cell Lung Cancer
Muyuan JIA ; Hongjun ZHANG ; Lin LI ; Jianhui WU ; Huanhuan GONG ; Bowen REN ; Han LIU
Cancer Research on Prevention and Treatment 2024;51(5):336-341
Objective To conduct a network meta-analysis on the effectiveness of first-line immunotherapy on patients with brain metastases from advanced non-small cell lung cancer (NSCLC). Methods Two investigators conducted a computerized search of Pubmed, Embase, Cochrane, and other databases to screen the literature, extract the information, and assess the risk of bias of the included studies. The included clinical trials were statistically analyzed using R (4.1.3) software. For the study outcome indicators OS and PFS, the risk ratios (
9.Study on the correlation of eosinophils and sinus CT findings in histology of chronic rhinosinusitis
Quanjie YOU ; Zhi LI ; Liang HAN ; Huanhuan YANG ; Hong FANG ; Yue ZHANG
Chinese Archives of Otolaryngology-Head and Neck Surgery 2017;24(12):633-636
OBJECTIVE To investigate the correlation between eosinophils(Eos) and CT findings in patients with chronic rhinosinusitis(CRS).METHODS The clinical data of 355 CRS patients who were treated at the First Affiliated Hospital of Wenzhou Medical University from January 2013 to June 2015 were studied.Friedman stage and Lund score and pathophysiological Eos count were recorded in all cases.The results were divided into eosinophilic CRS(ECRS) group and non-eosinophilic CRS(non-eosinophilic CRS,NECRS) group.The scores of CT findings of the two groups were compared and analyzed,and the correlation of the results of CT scores and the number of Eos in the tissue were explored.RESULTS There was a significant difference in total CT scores between ECRS and NECRS groups(Z=1.674,P<0.01),but there was no significant difference in CT scores among T1,T2,T3 and T4 stages(Z=1.011,0.892,0.612,0.80,P<0.01).And CT scores were positively correlated with Eos counts and percentages in the tissues (correlation coefficients r were 0.216,0.223,P<0.01).CONCLUSION 1.The difference of Eos in the tissue of the CRS is the cause of different CT manifestations.2.The more Eos count or the percentage,the higher the CT scores of patients with chronic sinusitis,which means the more severe of the CT imaging.
10.Effect of rhBNP on serum chemerin and IL-37 levels in acute myocardial infarction patients undergoing emergency PCI
Limin MENG ; Hua YANG ; Chao CHANG ; Shuanli XIN ; Xiufeng ZHAO ; Lijun LIU ; Liying HAN ; Min LI ; Huanhuan LI
Chinese Journal of Geriatric Heart Brain and Vessel Diseases 2018;20(4):378-381
Objective To study the effect of rhBNP on serum chemerin and IL-37 levels in acute myocardial infarction (AMI) patients undergoing emergency PCI.Methods Eighty AMI patients who underwent emergency PCI were randomly divided into cortrol group (n =40) and rhBNP treatment group (n=40).The patients in control group were treated with conventional drugs and those in rhBNP treatment group were treated with intravenous rhBNP.Their serum chemerin and IL-37 levels were measured by ELISA.Their LVEDD and LVEF were compared.Results The serum level of chemerin was significantly lower while that of IL-37 was significantly higher in two groups at 72 h and on day 7 after PCI than before PCI (P<0.05).The serum level of chemerin was significantly lower while that of IL-37 was significantly higher in rhBNP group than in control group at 72 h and on day 7 after PCI (P<0.05).The LVEDD was significantly shorter while the LVEF was significantly higher in two groups on day 7 and month 1 after PCI than before PCI (P<0.05).The LVEDD was significantly shorter in rhBNP group than in control group on day 7 and month 1 after PCI (P<0.05).Conclusion rhBNP can effectively reduce the serum chemerin level,increase the serum IL-37 level,and improve the cardiac function in AMI patients following emergency PCI.The effect of rhBNP is better than that of conventional drugs in AMI patients after emergency PCI.