Objective To observe the clinical efficacy of intratympanic dexamethasone injection for patients with sudden sensorineural hearing loss (SSNHL). Methods Forty-four SSNHL cases (44 ears) which failed to respond to regular treatments were divided into two groups. The intratympanic injection group (24 eases) were injected dexamethasone 2.5 mg to the tympanic cavity every two days,4 times in all. The control group (20 eases) were treated with B1,B12 and triphosaden. Pure tone average (PTA) was tested before and 3 days after treatment. Results The total effective rate of intratympanic injection group was higher than that of control group [37.5%(9/24) vs 10.0%(2/20)] (P < 0.05). In intratympanic injection group, PTA of post-injection was significantly lower than the pre-injection[ (52.75±20.14) dB vs (70.26±20.76) dB ] (P < 0.05). PTA of post-injection in intratympanic injection group was significantly better than that in control group[(52.75±20.14) dB vs (63.55±19.36) dB](P< 0.05). Conclusion Intratympanic dexamethasone injection can be applied to SSNHL patients who failed to respond to systemic corticosteroid treatment and it can avoid the side effects brought on by high dose systemic corticosteroid treatment.

Objective:To investigate the expression of focal adhesion kinase(FAK) and Ki67 in the invasive breast ductal carcinoma tissues, seeking their relationship with tumor invasion and metastasis and to study the mechanisms for cell signaling transduction mediated by FAK in the carcinogenesis and progress of breast carcinoma.Methods:Tissues were obtained from 88 patients with invasive breast ductal carcinoma and 25 with breast ductal epithelial benign proliferative disease. The expression of FAK and Ki67 was examined by immunohistochemical technique(streptavidin peroxidase conjugate method, SP).Results:In 88 cases of invasive breast ductal carcinoma, the positive incidences of FAK was 68.2%(60/88), and the Ki67 proliferation index was 29.6%?22.9%, which distinguished apparently to those of the control group(P0.05). In breast carcinoma tissues, the expression of FAK was positively related to Ki67 proliferation index(P

Humans ; Musculoskeletal Diseases ; psychology ; Occupational Diseases ; psychology ; Psychology, Social

To explore the mode of matrix and cell injury of articular cartilage subjected to high stress mechanical loads with a low rate of loading. Loads characterized by a constant stress rate of 0.26MPa/s to a peak stress between 2MPa and 11MPa being applied to the adult bovine articular cartilage explants was designated as the test groups. No mechanical loads were applied to the control group. All the explants were maintained in culture for the next few days after loads. The spatial patterns and severity of chondrocyte injury were determined using fluorescein diacetate and propidium iodide. Matrix damages were assessed by inspection of the microstructure of the collagen fiber network under SEM. When the peak stress was 5MPa and higher, the rate of cell death in the explants of test groups was significantly greater compared with controls, and the greater peak stress applied, the higher percentage of dead versus total cells was documented, and a more diffuse distribution of dead cells was observed. When the peak stress reached 9MPa, cell death involving full depth of the cartilage was observed in test groups . Damages to the collagen fiber network were not observed both in test and control groups. The results indicated that high stress mechanical loads with a low rate of loading resulted in more severe chonrocytes damage rather than collapse of collagen fiber network.

Objective To investigate the therapeutic effect of ipratropium bromide on bronchial spasm of patients with asthma and chronic obstructive pulmonary disease (COPD).Methods 174 patients with asthma and COPD were selected and divided into two groups, 87 cases in the control group treated with budesonide and formoterol fumarate powder for inhalation , 87 cases in the experimental group received ipratropium bromide on the basis of the control group, pulmonary ventilation function, airway resistance and cytokine levels in induced sputum, the clinical effect and incidence of adverse reactions were compared after the treatment.Results The effective rate in the control group(81.61%)was lower than the experimental group (93.11%), with significant difference (P<0.05); compared with the control group, levels of peak expiratory flow (PEF), maximum midexpiratory flow (MMEF), forced expiratory volume in one second to forced vital capacity ratio (FEV1%), forced vital capacity (FVC) were higher in the experimental group after treatment, levels of airway resistance (Raw), Raw% were lower, level of specific airway conductance (Gsp) was higher after treatment, induced sputum levels of vascular endothelial growth factor (VEGF), intercellular adhesion molecular-1 (ICAM-1), interleukin-13 (IL-13), IL-17 were lower after treatment, with significant difference (P<0.05);there was no significant difference in the incidence of adverse reactions between the two groups.Conclusion The clinical effect of ipratropium bromide in the treatment of asthma and COPD was exactly , ipratropium bromide can significantly improve the pulmonary ventilation function in patients, relieve bronchial spasm, reduce airway resistance, inhibit airway inflammation, and the safety is higher.

Purpose Glioblastoma and metastasis are the common and aggressive type of brain tumors in adults,and a definitive diagnosis has an important guiding significance to the clinical practice.Our purpose is to investigate the value of post contrast-enhanced T1WI-based histogram analysis in the differential diagnosis of glioblastoma and solitary brain metastasis.Materials and Methods Sixty-eight consecutive patients with glioblastoma or solitary metastasis confirmed pathologically or surgically at Meizhou People's Hospital between January 2012 and November 2015 were included in the study.Glioblastoma was diagnosed in 34 patients and solitary brain metastasis was diagnosed in the rest 34 patients.All patients had undergone routine brain MR examination before surgical resection and the imaging data were retrospectively analyzed.The ROIs were manually placed in enhanced solid parts of the tumors on the maximal cross-sectional post contrasted-enhanced T1WI by Image J.Then the mean,standard deviation (SD),minimum,maximum,skewness and kurtosis were calculated respectively.Results Kurtosis of post contrast-enhanced T1WI of metastasis (1.260 ± 1.271) was statistically higher than that of glioblastoma (0.071 ± 0.667)(P＜0.05).When the optimal threshold criterion ofkurtosis was set at 0.736,the areas under ROC curve of kurtosis was 0.792 (95％ CI:0.676-0.881),and the sensitivity,specificity,positive predictive value and negative predictive value at the optimum cutoff value were 76.47％,88.24％,80.65％,78.38％,respectively.Conclusion Kurtosis of post contrastenhanced T 1 WI-based histogram is valuable in differential diagnosis of glioblastoma and brain metastasis,and thus provides reliable and objective basis for differentiating the two kinds of tumors.

Adolescent ; Adult ; Capsules ; Drugs, Chinese Herbal ; therapeutic use ; Hepatitis B, Chronic ; drug therapy ; Humans ; Male ; Middle Aged ; Phytotherapy

Objective:To find out the key demand and connection point of health quality education for students of different stages by analysis of their health quality characteristics and the main contribution path, and then explore strategies to improve health quality. Methods:The stratified random cluster sampling method was carried out to select the sample population in Hangzhou. The variance analysis, curve fitting and multivariate linear regression analysis were used to analyze health quality characteristics and the main contribution path. Results:The health quality of pri-mary school students was best;the difference in quality between college students and junior high school students was not statistically significant ( P>0. 05 );and high school students had the worst health quality. The high school stage is the weakest link of health quality education. The order of the health education path and the means for improving the health quality of college, high school, and primary school students differ. With the improvement of students’ lev-el, the students that require physical quality promotion gradually decreases; the moral quality demand remains sta-ble;and the psychological quality and social adaptation ability present the tendency of a gradual increase. Conclu-sion:Student health quality shows a tendency to decreases with the rise of learning phase. The education departments should foster health consciousness and habits for students, form a healthy lifestyle behavior and ways of thinking for high school students, shape healthy personalities to improve health literacy and social adaptation abilities for college student, which is the key strategy for promoting the health quality of all-round development of the students and a-chieve the primary education goal of “health education”.

Objective To investigate the effect and mechanism of STAT3 gene silencing on the growth of xenografts in human pancreatic cancer SW1990 cells in nude mice.Methods The expression vector inserted with shRNA targeting at STAT3 gene was constructed and was stably transfected into SW1990 cells (SW1990-RNAi group).SW1990 cells transfected with negative control shRNA expression vector (SW1990-Con group) and parent SW1990 (SW1990 group) were used as controls.STAT3,VEGF,MMP-2 protein expressions in these groups were determined by using Western blot.The subcutaneous xenografts models were established in nude mice,and the growth of xenografts was observed,CD34 expressions were determined by immunohistochemistry and MVD was measured.Results The expression of STAT3 protein was 84.69 ± 9.31,82.00 ± 7.76,7.93 ± 1.24,repectively,in SW1990 group,SW1990-Con group,SW1990-RNAi group,and the expression of VEGF protein was 82.94 ± 8.97,80.86 ± 10.28,39.04 ± 6.23,respectively,and the expression of MMP-2 protein was 40.88 ± 5.09,38.26 ± 5.71,12.54 ± 2.15,respectively.The expression in SW1990-RNAi group was significantly lower than those in other 2 groups (P ＜ 0.05),while the expression of all three proteins between SW1990-Con group and SW1990 group was not significantly different.The weight of the xenografts in SW1990 group,SW1990-Con group,SW1990-RNAi group was (2.2 ± 0.4),(2.2 ± 0.3),(0.5 ± 0.3) g,respectively ; the MVD of the xenografts was (20.35 ± 2.41),(18.79 ± 1.94),(9.62 ± 1.06) per high power field,respectively,and the number in SW1990-RNAi group was significantly lower than those in other 2 groups (P ＜ 0.05 or P ＜ 0.01),while the difference between SW1990-Con group and SW1990 group were not significant.Conclusions Inhibition of STAT3 gene expression can significantly slow the growth of SW1990 xenografts in nude mice,and the mechanism may be related with down-regulation of VEGF and MMP-2 expression and inhibition of the angiogenesis of pancreatic cancer.

Chitinases genes from Metarhizium anisopliae which is an important entomopathogenic fungus were considered one of the key factors to invade their hosts. One Metarhizium anisopliae HN1 strain was isolated and screened. A chitinase gene was amplified by RT-PCR from Metarhizium anisopliae HN1, The whole length of this gene was 1275bp,and the nucleotide sequence of the gene was 96% similarity to that of the M. anisopliae E6 accessed in GenBank ( AF02749). The gene has been registered in GenBank and its accession number is DQ011865. The gene was subcloned into prokaryon expression vector pET-22b( + ), transformed this recombinant expression plasmid into E. coli strain BL 21 and effective expressed. The SDS-PAGE analysis indicated that the recombinant protein was 42kDa which is same to the reported article. The expression level of recombinant protein was about 63. 3% of whole expressed proteins , And when recombinant E. coli were crushed by freeze and supersonic wave , the activity assay indicates that the chitinase expressed in bacteria possesses biological activity.