BACKGROUND: Tissue engineering has a promising prospect in corneal transplantation. Scaffolds are always restricting the development of tissue-engineered cornea. OBJECTIVE: To analyze application of different seed cells and scaffolds, and to summarize the progress of tissue-engineered cornea in recent years.METHODS: First author searched literature from CNKI (2000/2010-10) and PubMed database (2000/2010-10). The key words are tissue engineering, corneal transplantation in Chinese or English. A total of 223 literatures were seized by computers, according to the inclusion criteria, papers concerning the advance, application and reconstruction of tissue-engineered cornea were analyzed. Finally, 33 papers were included for further analysis. The present study was to analyze the seed cells, scaffolds, organ building and clinical applications of tissue-engineered cornea and investigate the development direction in the future. RESULTS AND CONCLUSION: The results show that seed cells and scaffolds are the focus of the studies now. Corneal transplantation has a high success rate in organ transplantation since the immune privilege of eye and avascular cornea, and it will be able to be a tissue engineering organ that can be largely built, easy to transplant. Reconstruction of cornea has reached first base, but every kind of scaffold has certain drawbacks, so the next goal is to find an ideal scaffold material.

Making dental impressions and models is a part of daily routine work for dental nurses in the orthodontic department in China. This paper analyzes the common problems encountered in the process of making dental impressions and models, and emphasizes the importance of quality control and supervision during this process. A method of combining real-time monitoring and periodic inspection based on nurse-doctor cooperation was put forward to ensure the quality of dental impressions and models. Firstly, the nurses should be well trained with the instruction of doctors before on duty; Secondly, the head nurse should be responsible for implementing real-time monitoring and periodic inspections to find the problems and take measures in time. Also, the doctors should check the dental impressions and models in time, especially for those models with special demands, and help the nurses to improve their skills constantly.

To evaluate the role of nitric oxide in the embryo implantation in mice, three experiments were carried out using the mice implantation model. In the experimentⅠand experimentⅡ, NG-nitro-L-arginine methyl ester (L-NAME), the non-specific NOS inhibitor, was administered intraperitoneally at doses of 0.5 mg-5 mg on the day 3 of pregnancy and at dose of 3 mg on different days of pregnancy (day 1-6) . In the experiment Ⅲ, L-aragnine, a donor of NO, was co-administered with L-NAME to evaluate the effect of L-aragnine on L-NAME, and the embryo development was assessed. In all these three experiments, the endometrium was histologically examined. Results showed compared with the control groups intraperitoneal administration of a dose of L-NAME between 1 and 5 mg on the day 3 of pregnancy led to the decrease in the number of implantation sites (P<0.05), and 4 to 5 mg of L-NAME caused inhibition of implantation completely. L-NAME resulted in failure of pregnancy when administrated at 3 mg between day 3 and 5 of pregnancy. The characteristic vascular permeability changes and decidualization in the endometrium were significantly attenuated and embryo growth was retarded. The L-NAME-mediated effects were significantly reversed when L-aragnine was co-administered with L-NAME. This study demonstrated that NO promoted the implantation in mice through regulating the permeability and decidulization of endometrium and the development of embryo.

Objective To observe changes of plasma thrombinogen segment 1+2(FI+2)and D-dimer(D-D)in multiple trauma patients and exphlore their relations with traumatic disseminated intra-vascular coagulation(DIC). Methods A total of 66 multiple trauma patients were divided into minor injury group(ISS＜16 points,21 patients)and severe multiple trauma group(ISS≥16 points,45 pa-tients).Then.severe multiple trauma group were divided into DIC group(12 patients)and non-DIC group(33 patients).Another 10 healthy pemons were served as control group.Venous blood was collect-ed once in the control group and that collected from other groups at days 1,3 and 7 after injury.The F1 +2 concentrarion was detetrained with ELISA.and the D-D concentration was measured by automated la-tex enhanced immunoassay. Results The F1+2 and D-D levels in the minor injury group and sever multiple trauma group were both higher than that of the control group.In the meantime.the F1+2 and D-D levels in severe injury group were remarkably higher than that in the minor injury group.The plasma F1 +2 and D-D levels were elevated continuously in traumatic DIC group and remarkably higher than that in the non.DIC group.in which the plasma F1+2 and D-D levels gradually declined.Plasma F1+2 and D-D levels had significantly positive correlations at days 1,3 and 7 after injury. Conclusions Hiigher levels of F1+2 and D.D at acute stage is not only relevant to the injury severity,but also closely to the occurrence of traumatic DIC after injury.Detection of plasma F1+2 and D-D levels may play an impor-taut role in early prediction of DIC.

The incidence of invasive candida infection in critically ill patients is rising year by year. It's clinical manifestation lacks specificity which is easy to result in missed diagnosis or misdiagnosis.Tradi-tional diagnosis methods need very long time and have low positive rate,which are easy to delay treatment,so early diagnosis is particularly important.1 ,3-β-D-glucan,mannan and antibodies,anti-enolase antibodies,can-dida score and colonization index may be helpful to make early diagnosis.

Purpose: To observe the clinical therapeutic effect in the treatment of enuresis by ginger-partitioned moxibustion plus cupping therapy. Methods: Ginger-par -titioned moxibustion was applied on Guanyuan (CV 4)and bilateral Zusanli (ST 36) and cupping therapy was applied on Shenque (CV 8) in the treatment of 82 cases of enuresis, in comparison with 76 cases treated by Chinese herbal medicine. Results: The effective rate was 84.1% in the treatment by ginger-partitioned moxibustion plus cupping therapy and was 64.5% in the treatment by Chinese herbal medicine. Conclusion: Ginger-partitio -ned moxibustion plus cupping therapy was better than Chinese herbal medicine in the treatment of enuresis (P ＜0.05).

In the treatment of 80 cases of the patients with deficient and cold pattern in the spleen and stomach by external application of herbal paste on the acupoints, the results showed remarkable effect in 52 cases, improvement in 24 cases, failure in 4 cases and the total effective rate in 95.0%. Remarkable therapeutic effects were achieved in three different diseases of same pattern. External application of herbal paste on the acupoints is definitely effective for stomachache in deficient and cold pattern of the spleen and stomach.

Objective To explore the clinical application of function-preserving pancreatic surgery for treating pancreatic neuroendocrine neoplasms (pNENs).Methods Twenty-eight patients with pNENs treated by the function-preserving pancreatic surgery at Anhui Province Hospital from January 2002 to December 2015 were analyzed retrospectively.Results Among the 28 patients, enucleation surgery was performed in 18 cases, distal pancreatectomy was performed in 8 cases, and middle pancreatectomy was performed in 2 patients.Total average operation time was (197±68) min, and the blood loss was (106±99) ml.The postoperative pancreatic fistula was observed in 7 patients, seroperitoneum was in 4 patients, incisional infection was in 1 patient, pulmonary infection was in 2 patients, and all the patients were cured by conservative treatment.There was no death during perioperative period.The postoperative hospital stay was (13±6)d.Analysis of pathological examination and clinical symptoms showed that 24 cases were diagnosed as functional pNENs (including 23 with insulinoma and 1 with gastrinoma), and 4 cases were as non-functional pNENs.The tumor grade G1 was categorized in 19 cases, G2 was in 9 cases.The TNM was I stage in all patients.There were no vascular or nerve invasion or lymph node metastasis.The follow-up period ranged from 4 to 144 months.2 patients were lost, and other patients were all alive.No diabetes or hypoglycemia occurred.Conclusions The function-preserving surgery for pNENs was safe and feasible, especially for well-differentiated functional pNENs.

As a new laboratory test for evaluation of endogenous pain inhibition,conditioned pain modulation (CPM) deficiency means dysfunction of endogenous pain inhibitory systems and higher incidence of chronic pain.Age,psychological factors and physical activity all seem to influence the individual CPM effect.A standard CPM testing way has an important role in comparison between different researches.

MicroRNAs are a class of small (-22nt) non-coding RNAs that bind to the 3' untranslated regions (3'-UTRs) of their target mRNAs in a complementary or partially complementary manner via the seed sequence in their 5'-region to regulate biological effect. MicroRNAs also expressin malignant tumors and have close relations with occurrence, development and other biological characteristics of tumor. The effect of radiotherapy and the prognosis of cancer patients are limited and influenced by radioresistant all the time. In recent years, the application of microRNAs to improve the radiation sensitivity of tumor cells is a new field in tumor biotherapy. This paper mainly reviews the identification of related microRNAs participating in and regulating the formation of radiosensitivity/radioresistent, and the research progress of their possible mechanisms.
Humans ; MicroRNAs ; metabolism ; therapeutic use ; Neoplasms ; metabolism ; radiotherapy ; Prognosis ; RNA, Messenger ; metabolism ; Radiation Tolerance