Objective To study the protective effect of leuprorelin particle on ovarian reserve function applied before the laparoscopic cystectomy of ovarian endometriosis.Methods 106 patients with endometriosis treated from January 2015 to December 2015 in our hospital were selected.The subjects were divided into two groups according to the random number table, 53 cases of each group.The control group adopted the laparoscopic surgery;the observation group took the leuprorelin particle before the laparoscopic surgery.The operation condition, ovarian hormone and function were observed. Results The operation time and bleeding amount in observation group was better than control group ( P <0.05 ) .After surgery, the exhaust time between two groups had no significantly different.After surgery, the level of follicle stimulating hormone (FSH) in observation group was lower than control group; the level of estradiol (E2) in observation group was higher than control group (P <0.05); the level of luteinizing hormone (LH) between two groups had no significantly different.After surgery, the ovarian volume and antral follicle count ( AFC) in observation group was better than control group (P<0.05).Conclusion Before the laparoscopic cystectomy of ovarian endometriosis, the leuprorelin particle protects the ovarian function and morphology and has less impact in the level of hormone.

Endothelial Cells ; metabolism ; physiology ; Stem Cells ; metabolism ; physiology

Heart Failure ; therapy ; Humans ; Practice Guidelines as Topic

OBJECTIVE:To investigate the correlation of serum resistin and adiponectJn Ievels with bone mineral density(BMD)in 232 healthy male volunteers in Changsha area.METHODS:A totaI of 232 healthy Chinese men of Han population were selected randomly from Changsha area.All subjects gave their informed consent to participate in this study.Subjects were excluded from the study if they had conditions or received treatment that affecting bone metabolism.Serum adiponectin and resistin levels were determined by ELISA.BMD was measured by dual energy X-ray absorptiometry in total body,lumbar spine,and total hip.Body fat mass and lean body mass were measured by the whole body scanning.The correlation of serum adiponectin and resistin levels with fat mass and BMD at various sites were analyzed;the predictors of BMD at various sites was assayed using multiple linear stepwise regression analysis.RESULTS:Adiponectin was negatively correlated with fat mass(r=-0.216.P＜0.05),while resistin had no correlation with fat mass.The correlation disappeared after adjustment for age and fat mass(r=-0.006,P＞0.05).The significant negative correlations between adiponectin and BMD were found,and remained significant after adjustment for age and fat mass.There was no correlation between resistin and BMD at various sites.In the multiple linear stepwise regression analysis.adiponectin was an independent predictor of BMD in men.CONCLUSION:Resistin js not correlated with fat mass and BMD at various sites.Adiponectin is correlated with fat mass,and has a negative correlation with BMD at various sites,it is an independent predictor of BMD in Chinese men.

BACKGROUND:Compared with the bone marrow mesenchymal stem cells (BMSCs),cells from the umbilical cord blood (UCB)are considered"very young",and its proliferation and differentiation cannot decrease with age.Thus,cells from UCB are a great substitute of BMSCs.OBJECTIVE:To observe the characteristics of in vitro differentiation of UCB-MSCs into cardiomyocytes.DESIGN,TIME AND SETTING:The observational experiment was performed at the Beijing Sijitan Hospital from March 2005 to February 2007.MATERIALS:Cells from UCB were collected from healthy arturients after signing the informed consent.METHODS:Mononuclear cells were isolated to further harvest MSCs.At the third passage,CD34,CD44 and CD90 were measured using immunofluorescence flow cytometry.After 4 weeks of 5-azacitidine treatment,myosin heavy chain,GATA4 and troponin 1 were identified using immunohistochemistry and reverse transcription-polymerase chain reaction.MAIN OUTCOME MEASURES:β-myosin heavy chain,GATA4 and troponin I expression.RESULTS:UCB-MSCs showed a fibrolast-like morphology,clonally expanded after 5-azacitidine reatment.The immunophenotype of these clonally expanded cells is consistent with that reported for BMSCs.Immunohistochemistry and reverse transcription-polymerase chain reaction showed β-myosin heavy chain,GATA4 and troponin I expression.CONCLUSION:UCB-MSCs differentiate into cardiomyocyte-like cells,which have the characteristics of cardiomyocytes.

Objective　To investigate the early changes and clinical significance of plasma endothelin(ET) and nitric oxide(NO) in patients with acute brain injury.　Methods　The plasma ET and NO concentration at 1 day after injury were checked with radioimmunoassay and Green method in 48 cases of acute brain injury, in 42 cases of non-brain injury and in 38 healthy volunteers. 　Results　 The early plasma concentrations of ET and NO were (109.73±12.61) ng/Lf and (92.82±18.21) μmol/L, respectively, in 48 cases of acute cerebral injury, which were higher than those in the non-brain injury group［(67.90±11.33) ng/L and (52.66±12.82) μmol/L, respectively, P<0.01］ and in the normal control group ［(50.65±17.12)ng/L and (36.12±12.16) μmol/L, respectively, P<0.001］. The amounts of ET and NO in the patients with severe brain injury were (116.18±18.12) ng/L and (108.19±13.28) μmol/L, respectively, which were significantly higher than that of the moderate brain injury group［(92.33±16.32) ng/L and (76.38±12.71) μmol/L, respectively, P<0.01］.The early levels of ET and NO were negatively related to the GCS scores of the patients with acute brain injury. The amounts of ET and NO were (126.23±15.23)ng/l and (118.18±10.12) μmol/L, respectively, in the patients with subdural hematomas, which were significantly higher than those in the patients with epidural hematomas ［(81.13±12.37) ng/L and(68.02±13.18) μmol/L, respectively, P<0.01］. The stable plasma concentrations of ET and NO at 14 days after injury in the patients with acute brain injury and with non-brain injury were similar to that in the normal controls. 　Conclusions　 ET and NO are related to the pathophysiological process in the early period of acute brain injury, and the values of ET and NO correlate positively with the clinical manifestations. The changes of plasma ET and NO can be regarded as important indexes to diagnose the severity of acute brain injury.

BACKGROUND: Wear particles factually serve as a medium carrying extensive information on the wear process involved in hip implants. Moreover, it has been demonstrated that wear particles contribute to the failure of Charnley total hip replacement.OBJECTIVE: To investigate the morphological characteristics of wear particles generated by couples of ultrahigh molecular weight polyethylene (UHMWPE) cups and Al2O3 femoral heads, mounted in a simulator in the presence of distilled water and explore the wear mechanism of UHMWPE cup.DESIGN: Wear tests were performed on a ball-in-socket hip simulator.SETTING: Department of Mechanical Engineering, Xuzhou Institute of Technology.MATERIALS: The molecular weight of UHMWPE selected as cup was 3 000 000. Its density was 0.935 g/cm3, hardness 30 HB, fracture strength 35 MPa, and water absorption < 0.01%. The experimental femoral head was made of alumina and zirconia (Al2O3: 87%, ZrO2: 13%). Its density was 4 g/cm3, hardness 90 HRA, tensile strength > 450 MPa, and tenacity 7 MPaM1/2.METHODS: The experiment was performed in the laboratory of tribology, China University of Mining and Technology from December 2003 to July 2005. Hip simulator was used to assess the tribological performance of Al2O3-UHMWPE at room temperature, 100 r/min, 784 N load for 1.5×106 cycles. Distilled water was used as the lubricant. Wear of UHMWPE acetabulum was measured every 3×105 cycles by taking out the acetabulum from simulator, cleaning in distilled water for 3 minutes in an ultrasonic bath, putting in an oven for 40 minutes at 80 ℃, and cooling off in a desiccator. A BT211D electronic balance (reciprocal sensibility, 0.01 mg) was used to measure the weight loss of UHMWPE acetabula according to the reference sample method to calculate the wear. A sample (20 mL) of distilled water lubricant used in test was obtained when the simulator was stopped for gravimetric analysis. Morphology of wear particles in samples and surface of worn UHMWPE acetabula were observed by using scanning electron microscope (SEM). The components of wear particles were determined by X-ray. Detailed measurements of the wear particles distribution were conducted by LS100(R) Laser Particle Size Analyzer (including LS SVM Plus).MAIN OUTCOME MEASURES: Morphology and size distribution of wear particles; Wear mechanism and wear rate of UHMWPE acetabula.RESULTS: The wear particles showed a wide range of particle sizes, varying from 0.3 to 180 μm, most of which ranged from 5 to 20 μm. More than 99% of wear particles were UHMWPE particles, which were classified into five groups in terms of their morphological characteristics: spherical particle, tearing particle, lamellar particle, fatigued flaking and rod-shaped particle. Lamellar or cube was the predominant form of Al2O3 ceramic particles with flat surface structure and upright edges. The worn surfaces of UHMWPE cup showed evidence of multidirectional scratching, adhesive and micro-contact fatigue wear, and plastic deformation.CONCLUSION: ①The dominant wear mechanisms acting in the UHMWPE cup are abrasive wear, adhesive wear, microcosmic fatigue wear and flaking associated with plastic deformation. ②The size of particles accumulating mostly is tiny among the particle groups.

Objective　To measure the levels of urokinase-type plasminogen activator (uPA) and its receptor (uPAR) in synovial fluid and plasma of patients with RA and investigate its clinical significance.Methods　By using ELISA sandwich method,the levels of uPA and uPAR in plasma from 46 patients with RA and 8 with osteoarthritis (OA) were measured.Those in synovial fluid (SF) from 14 patients with RA,and plasma from 12 healthy subjects were measured as controls.Results　The concentration of uPA and uPAR in SF from patients was significant higher than that in their plasma (P＜0.001,P＜0.01),and the concentration of uPA and uPAR in plasma of RA was also much higher than that in plasma of OA patients (P＜0.05,P＜0.000 1) and healthy subjects (P＜0.000 1,P＜0.001).There was no significance between plasma levels of RF＋ and RF－ in RA patients.The concentration of uPA and uPAR of plasma in RA correlated positively with CRP,RF and the number of swelling joints.Conclusion　The levels of uPA and uPAR in SF and plasma are useful parameters for monitoring disease activity of RA.These findings suggest that the uPA and uPAR genes may play an important role via proteolytic damage of the extracellular matrix during the development of RA.

ObjectiveTo study the heat shock protein 47 ( HSP 47 ) andtype Ⅰ ,Ⅲ collagen relevance expression in keloid tissue. MethodsUSE immunohistochemistry to detect 21 patients( HS group) pathological keloid tissue HSP47, Ⅰ-type, Ⅲ collagen levels,and normal foreskin(control group) compared to the expression of HSP 47 and Ⅰ and Ⅲ collagen. ResultsHS group HSP 47 scar absorbance was( A value) ( 133.0 ± 58.7) ,it was significantly lower than the control group( 169.6 ± 53.2) ( t =2. 697 ,P =0.024) ;two groups of collagen type Ⅰ, Ⅲ collagen levels had statistically differentce(t =14.898,12.610,all P =0.000) ; HSP 47 level and type Ⅰ collagen were positively correlated(r =3.058,P =0. 034),and type Ⅲ collagen content was also positively correlated(r =4. 610, P =0. 029). ConclusionHSP 47 in keloid tissue could cause dermal layer assembly of collagen synthesis and significantly increased,and it was an important mechanism for the formation of keloids.

Objective To investigate the efficacy and safety of tranexamic acid in perioperative blood loss in old patients with multi-level lumbar spinal stenosis.Methods From January 2009 to September 2010,a total of 68 consecutive patients with multi-level lumbar spinal stenosis ( ≥65 years old) underwent posterior decompression,internal fixation and bone graft fusion who were randomly divided into group A and group B with 34 patients in each.The patients in group A received tranexamic acid and the patients in group B received an equal volume of normal saline.The amounts of intraoperative blood loss,postoperative wound drainage,blood transfusion,the number of the patients needing blood transfusion and hemoglobin,fibrinogen,prothrombin time and so on were examined preoperatively and 24 hours postoperatively.All the patients were observed for the clinical symptoms of deep vein thrombosis.Results The amounts of intraoperative blood loss,postoperative wound drainage,blood transfusion and the number of the patients needing blood transfusion in group A were significantly decreased than those in group B[ (641.1 ± 128.4) ml vs.(780.1 ± 107.3) ml,(228.80 ± 52.07) ml vs.(345.50 ±42.16) ml,(1.02 + 1.56) U vs.(2.89 ± 1.76) U,16 cases vs.28 cases ],there were significant differences between two groups (P ＜ 0.05 ).As for the value of postoperative hemoglobin concentration in group A [ ( 104.00 ± 4.87) g/L ] was significantly higher than that in group B [ (92.20 + 5.47 ) g/L ] (P ＜ 0.05 ).There were no significant differences in the levels of fibrinogen,prothrombin time,and activated partial thromboplastin time between two groups (P ＞ 0.05).No deep vein thrombosis was found 7 days postoperatively.Conclusion Tranexamic acid can be effectively used in spine surgery in old patients with multi-level lumbar spinal stenosis without increasing the risk of venous thrombosis.