Objective To investigate the bacterial distribution and drug sensitivity characteristics of chronic suppurative sinusitis.Methods Nasal secretions of 89 cases of chronic suppurative sinusitis patients before operation were collected in this study,and nasal secretions were culture in medium.Disk diffusion method (Kirby-Bauer,K-B)was used for drug sensitivity test.Sensitivity of bacteria strain to cefazolin,ceftazidime,cefepime,imipenem,mero-penem,vancomycin,cefoperazone -sulbactam,ciprofloxacin and other antibiotics were analyzed.Results After the submission of purulent secretion specimens of nasal meatus in 89 cases chronic suppurative sinusitis patients with sticky,we found that 80 of the patients were detected bacteria.The positive rate was 89.89% (80/89).80 patients were detected in 82 strains of bacteria,a total of 15 species,of which 1 cases of nasal meatus sticky purulent secretion specimens there were 2 strains of bacteria.In 15 kinds of staphylococcus aureus were detected bacteria,staphylococcus aureus,escherichia coli,pseudomonas,enterobacter aerogenes,5 kinds of bacteria occupy the top 5,respectively, accounted for 21.95%,18.29%,14.63%,13.41%,10.98%.Drug sensitivity test of staphylococcus aureus showed that all of the staphylococcus aureus was sensitive to vancomycin,the sensitivity was 100%;the sensitivity of cefazo-lin,ceftazidime,cefepime,imipenem,meropenem,cefoperazone sulbactam,ciprofloxacin and other antibiotics were 0.00%.All the staphylococcus epidermidis were sensitive to imipenem,meropenem,vancomycin,cefoperazone sulbac-tam,the sensitivity was 100.00%;followed by cefazolin,the sensitivity was 86.67%,and to ceftazidime,cefepime, ciprofloxacin sensitive,were all below 30.00%.Escherichia coli,pseudomonas aeruginosa and enterobacter aerogenes and other gram negative bacteria were sensitive to imipenem,meropenem,the sensitivity was 100.00%.Conclusion The bacterial infection in patients with chronic suppurative sinusitis pathogens,including staphylococcus epidermidis, staphylococcus aureus,escherichia coli,pseudomonas,enterobacter aerogenes,bacterial culture positive to vancomy-cin,imipenem,meropenem,cefoperazone Shubatan,cefazolin and antimicrobial susceptibility,and so on antimicrobial treatment but should closely monitor the antimicrobial spectrum of change,in order to adjust the medication.

In the treatment of 100 cases of prolapsed lumbar intravertebral disc mainly by electric acupuncture on Jiaji( Ex-B 2) plus traction, the total effecrive rate was 91%.

FoxO as an important transcription factors,can be deacetylated by SIRT1 (a sort of deacetylases),SIRT1-FoxO-au-tophagy pathway is likely to play a vital role in some diseases,e. g.diabetes,aging,obesity,cancer,cardiovascular diseases and muscle atrophy,etcetera.This paper aims to analy transcription-al regulation mechanism of FoxO,and reviews the progress of SIRT1-FoxO-autophagy pathway.

Magnetic iron oxide nanoparticles (MIONs) have been widely used in biomedical fields, including targeted drug deliv-ery, magnetic resonance imaging, separation and enrichment of cancer cells, and tumor-targeted therapy. However, MIONs are increas-ingly necessary to ensure reduced toxicity, more stable colloidal dispersion, better biocompatibility, and higher magnetic responsive-ness. Thus, apart from the continuous improvement of material synthesis, specific surface modification of MIONs is essential to choose appropriate materials. This paper reviews the available methods and materials and their function in MION surface modification as well as their applications for the separation of circulating tumor cells.

For hypertension,lowering blood pressure is the basis and well controlled pressure is the core.The ultimate goal of the therapy is to decrease the risk of target organs damage,then minimize the occurrence of cardiovascular endpoint events.Evidence based medicine has confirmed the advantage of ACEI and CCB therapy in controlling blood pressure,lowering the incidence and the mortality of coronary disease.

Objective To evaluate the clinical value of fetal holoprosencephaly complicated with face malformation by two-dimensional(2D) and three-dimensional(3D) ultrasonography.Methods Fetal faces and brains were examined regularly with 3D ultrasonography when they were suspected abnormal with 2D ultrasonography and compaired the diagnoses with postnatal or induced delivery results.Results 11 cases of holoprosencephaly complicated with face malformation were diagnosed using 2D and 3D ultrasonography.Among these cases,there were 4 with single median mostril,2 with proboscis,2 with cheiloschisis,2 with multipte face malformation,1 with arhinia.They were all comfirmed by postnatal or induced delivery.Conclusions 3D ultrasonography provides visualized,three-dimensional,more precise and clearly fetal structure imagine.It has important supplement to 2D ultrasonography.2D ultrasonography combined with 3D ultrasonography can greatly enhance the diagnosis rate to holoprosencephaly complicated with face malformation.

Objective To establish an animal model of passive transferred myasthenia gravis(PTMG) in C57BL/6 mice aged 4 to 5 weeks with nicotinic acetylcholine receptor monoclonal antibody-mAb35 so as to decide the effective dose of mAb35 required to induce PTMG and to supply animal model for further study of the pathogenesis and immunotherapy of human MG.Methods The C57BL/6 mice aged 4 to 5 weeks in the experimental group were divided into three subgroups(E1,E2,E3),injected intraperitoneally(i.p.) with 0.2 ml of Ringer's buffer solution containing 0.5,1.0 or 1.5 mg/kg capital monoclonal antibody mAb35 of acetylcholine receptor respectively.The control group(N) were injected i.p.with 0.2 ml of Ringer's buffer solution without mAb35.To decide whether the model was successful,the clinical symptoms,ultrastructural changes of the mice as well as pharmacological and electrophysiological evaluation were observed and the serum levels of acetylcholine receptor antibody(AChRAb) were detected.Results All mice in the experimental groups were induced of clinic symptoms of myasthenia.The features in pharmacology,electrophysiology,ultrastructure and increased level of acetylcholine receptor antibody were in accordance with those of MG patients.Conclusion The model of PTMG can be successfully induced in C57BL/6 mice with mAb35 at effective dose of 1.0 mg/kg.The method is convenient and reliable.A fine animal model is established for experimental study of myasthenia gravis in children.

H~3-thymidine was used in determining the neuronal mitosis of the traumati cerebral cortex in rats. Intraperitoneal injection of H~3-thymidine was given to the rats immediately after corticectomy of the parietal lobes. On the 4th day after operation, the cerebral tissue was removed, fixed in Carnoy, serial sectioned, and stained with cresyl violet.The results showed that in the surrounding area 100—200?m from the traumatic site, 2-5％ of the various types of cells in the cerebral tissue were labeled, i. e. silver granules were distributed within the nuclei. Macrophages, endothelial cells and pericytes of vessels make up most of the 2—5％, whereas neuroglia and neuron rank next. The nuclei of the majority of the labeled cells were in the interphase state and a few of them were in the mitotic state. The more the labeled cells are in the interphase state,, the more the mitosis occur. On the contrary, it is less. They are always in direct proportion. In the case of neurons the same situation occurs.By inference, the mitotic division of the neurons in the cerebral cortex may occur in situ. Possibly some kind of substance is produced after the trauma leads to the division of the mature neurons. The basis for this inference is that a certain number of the neurons in division have typical features of the pyramidal neurons.

The plasmids of recombinant suicide vector containing the truncated stn gene and the deleted stn gene were constructed. The Salmonella stn-deficient mutant was prepared by homologous recombination between the mutant stn gene in the recombinant suicide vector and the wild-type gene in the chromosome of S. Typhimurium 2000. The detection of biological activity of stn gene production in those mutants indicated that Salmonella stn-deficient mutants evoked significantly less fluid secretion in mouse intestinal loops compared to that seen with wild-type Salmonella. Upon oral challenge of mice, the fifty percent lethal dose of the Salmonella stn-deficient mutants was greater than that for the wild-type bacteria. Those studies showed that the stn gene is very important factor in the pathogenesis of salmonellosis.

Chylous Ascites ; congenital ; therapy ; Humans ; Infant ; Male ; Parenteral Nutrition, Total ; Somatostatin ; therapeutic use