Objective To investigate expression of PP2CE in human hepatocellular carcinoma(HCC)tissues and the clinical pathological significance,and test its sub-cellular localization in liver cells and HCC cells.Methods A total of 50 samples of HCC tissues and their para-carcinoma tissues were collected in Tongji Hospital(Wuhan).PP2CE mRNA and protein levels were detected in HCC tissues and their para-carcinoma tissues by qRT-PCR and immunohistochemistry method.Immunofluorescent assay was performed to test the sub-cellular localization of PP2CE in the cells.Results PP2CE mRNA and protein expression levels were significantly decreased in HCC tissues as compared with those in para-carcinoma tissues(both P＜0.05).Immunohistochemistry analysis found that PP2CE expression was associated with tumor size,differentiated degree,portal vein invasion and clinical stage(both P＜0.05).Immunofluorescent assay suggested that PP2CE was mainly located in the cytoplasm and perinuclear area.Conclusion PP2CE is reduced in HCC and may be implicated in the development and progression of HCC.

The protecting effects of HZD, DZ and Mg~(2+) on isolated cultured rat li ver cells injured by CCl_4 was compared in this article. The results showed that HZD (15?l/ml)or DZ(10~(-4)M)or Mg~(2+)(4.8?10~(-3)M)added to the liquid culture of rat liver cells, inhibited the overloading of calcium ions and the leakage of LDH and GOT in hepatocytes effectively. The contents of Ca~(2+) ion were positively related to the leakage of enzymes.The intensity of inhibition effects to the calcium overloading and the enzyme leakage in HZD were situated between those in DZ and Mg~(2+).

Objective To investigate the stability and comparability of recombinant human muscle creatine kinase isozyme(CK-MM)in different matrix,and to find out a suitable matrix as a reference material for application of CK measurement system.Methods The stability of the recombinant CK-MM was observed by measuring its catalytic activity in self-made matrix,anti-freeze additive matrix,the certified reference material(CRM)matrix and human serum matrix respectively.For understanding the commutability among different measurement system total CK catalytic activity in various concentration of human serum and the catalytic activity of recombinant CK-MM in different matrix specimen were measured with IFCC recommended reference method and routine reagent produced by Roche and Zhongsheng Diagnostic Company respectively.Results The recombinant CK-MM was stable for 25 days in the self-made matrix,23 days in CRM matrix and at least 240 days in antifreeze additive matrix stored at-20 ℃.The recombinant enzyme protein in self-made matrix and CRM matrix was commutable.Low concentrations of recombinant CK-MM in human serum and in anti-freeze additive matrix were also commutable.Conclusion The recombinant CK-MM had good stability and commutability in either self-made matrix or CRM matrix.It might be used as a candidate reference material for the determination of total creatine kinase in serum.

OBJECTIVE:To investigate the development of implants for internal fixation of spinal tuberculosis,and to evaluate the clinical effect and biocompatibility. METHODS:Articles were retrieved from CNKI and Medline database from 1998 to 2008 with the of "spinal tuberculosis,thoracic spine,internal fixation,bone fusion,biocompatibility" in both Chinese and English. Among 50 retrieval articles,20 were included in the final evaluation according to inclusion and exclusion criteria. This study was designed to summarize therapy development of spinal tuberculosis and selection of implants for internal fixation,and to investigate clinical development of spinal tuberculosis therapy,types of implantation,biocompatibility of implants,and complications. RESULTS:Spinal tuberculosis treated by debridement bone graft and internal fixation characterized by reconstructing spinal stability,enhancing bone graft fusion ratio,reducing relapse rate,and preventing loss of corrected angle degree,thus it became a tendency for treatment of spinal tuberculosis. Implants which were implanted in focus of spinal tuberculosis were safe because they could not cause infection diffusion or formation of tuberculose focus in the local region. Otherwise,the implants had a good biocompatibility to host. Individualized program was chosen according to lesion degree and site in order to thoroughly clean focus,reconstruct spinal stability,and provide a stable environment for rehabilitation. Implantation of autologous ilium and titanium-net cancellated bone were widely used on clinic,and both of them had an excellent fusion ratio. Artificial bone did not have immunological rejection to organism,and it was gradually degraded and provided sufficient bone source. CONCLUSION:Spinal tuberculosis treated by debridement bone graft and internal fixation is an effective method to treat severe spinal tuberculosis. At present,autologous ilium and titanium-net cancellated bone are frequently used for clinical internal fixation,because both of them have good biocompatibility. Artificial bone characterizes by well clinical effect and biocompatibility,but the long-term effect and safety need to be further studied.

Objective] Explain the intentions of those famous TCM combinations for disease according to Shennong Bencao Jing. [Methods] Find out the principles to make up those famous TCM prescriptions based on understanding the efficacy of medicine described in Shennong Bencao Jing, through combining comparing the original documents of each other with the clinical practice of Mr. Xu Xiaodong. The natural medicine such as Gan Dihuang, Baizhu and Chai Hu were used as mediums to relate those famous TCM prescriptions and Shennong Bencao Jing. [Results] The original documents of Shennong Bencao Jing and those famous TCM prescriptions which were related together by natural medicine such as Gan Dihuang, Baizhu and Chai Hu have much in common. For example, Gan Dihuang used in Si Wutang, Huang Tutang etc. according to the effect of promoting blood circulation and removing blood stasis described in Shennong Bencao Jing. Chang Pu used in Changpu Yujintang according to the effect of resuscitation and restoring consciousness described in Shennong Bencao Jing. Baizhu used in Fangji Huangqi Tang, Mahuang Jiazhu Tang of Jinkui Yaolue according to the antiperspirant effect described in Shennong Bencao Jing. Chai Hu used in Xiao Chaihu Tang, Chaihu Shugan San etc. according to the effect of relieving the abdominal discomfort, attacks of chills and fever described in Shennong Bencao Jing. The theory of Shennong Bencao Jing is well applied in clinical teaching and practice by Mr. Xu Xiaodong, bringing students a lot of inspiration. It is possible to explain the principles of making up those famous TCM prescriptions and guide the compatibility of TCM according to Shennong Bencao Jing. [Conclusion] Doctors in the ancient world have inherited and developed the Shennong Bencao Jing theory when filling the prescription; comprehension and application of those famous TCM prescriptions will be more original according to Shennong Bencao Jing. The theory of Shennong Bencao Jing is worth our further study and extension.

Objective To investigate the early diagnosis of sudden cardiac death so as to increase the resuscitating success rate.Methods 24 cases with sudden cardiac death were analyzed.Results In 24 cases,there were 10 cases resuscitated successfully,8 cases with heartbeats recovered died in 1～3 days,5 of which had average blood glucose of 21.6 mmol/L and blood sodium of 156 mmol/L,and 6 cases resuscitated unsuccessfully.Conclusion 70.83% was caused by AMI in 24 cases.The early diagnosis of AMI is mainly based on clinical manifestation.Althogh early ECG did not show it,AMI should not be denied and misdiagnosed.Resuscitation should be performed on the spot as soon as cardiac arrest occurs.Naloxone and NaHCO3 should be used rationally.Meanwhile,be sure to prevent and treat reperfusion-injury.The prognosis would be extremely poor if the patient showed hyperglycemia and hypernatremia during resuscitation.

icating that Fas/FasL plays an impertant role in the regulation of immune/inflammation response in the CNS, and such role does not depend on the apoptotic pathway, This article reviews its progress in research on the inflammatory response in the CNS.

indicators of oxidative stress.

Objective To observe the effects of esophageal stricture treated with esophageal stenting.Methods 23 esophagus stenosis patients were expanded to 1 lmm,and the stent was implanted-.Results 23 patients were set into successfully,7 cases could enter the common food,11 caces could enter the soft food,5 cases could enter the semiliquid food.18 cases had the ache,6 cases had the stomach esophagus regurgitation symptom.During 1?12 month follow-up,10 cases survived,13 cases died,the cause of death was mainly later period cancer failure and metastasis.Conclusion Esophagus stenosis treatment operation was simple,safe,and the wound small,the short-term curative effect would be good.

Objective Psychological intervention combined with carboprost ammonia butyl alcohol three for pregnancy clinical effect in the treatment of postpartum hemorrhage and its impact on quality of life. Methods According to the different nursing methods from January 2015 to December2016 in our hospital with high risk of postpartum hemorrhage patients were divided into 50 groups: control group with routine nursing care plus carboprost ammonia butyl alcohol three treatment, the observation group with psychological intervention plus carboprost ammonia butyl alcohol three treatment; comprehensive observation of the two groups of 2h and 24h in patients with postpartum hemorrhage, bleeding time, quality of life and other aspects, and the related data were analyzed. Results The psychological intervention plus carboprost ammonia Butyl alcohol three (observation group) carboprost ammonia butyl alcohol three with better effect than the treatment of postpartum hemorrhage pregnancy (control group) treatment, 2h and 24h in patients with postpartum bleeding was less than the control group, the bleeding time was shorter than the control group, the quality of life is better than the control group, the difference was statistically significant (P<0.05). Conclusion Postpartum hemorrhage: Patients with psychological intervention plus carboprost ammonia butyl alcohol three treatment effect of pregnancy, can decrease the 2h and 24h in patients with postpartum hemorrhage, bleeding time is short, the quality of life of patients, is worthy of clinical widely used to treat patients with postpartum hemorrhage pregnancy.