Purpose:It has been shown that the heart is a chaotic oscillator. So it is appropriiate to use the Lyapunov exponent, an important parameter to identify the nature of non-linear dynamical systems, for identifying the state of human heart. Methods:Preliminary results are obtained in this paper using Wolf's algorithm for 8 normal and 107 abnormal ECG recordings. Results:Significant differences are found between the Lyapunov exponents of normal ECG and ECG with obvious coronary stenosis (OCS), but there is no significant difference between the Lyapunov exponents of normal ECG and ECG with mild coronary stenosis (MCS);Significant differences are also found between the Lyapunov exponents of R-R interval series of normal ECG、ECG with MCS and ECG with OCS. Conclusions:It is apparent that the R-R interval series can give us more messages about human heart, and the Lyapunov exponents of ECG and R-R interval series are the appropriate parameters for the identification of the physiological states of human heart. It is possible to use Lyapunov exponent for early diagnosis of Coronary Heart Disease.

OBJECTIVE To investigate pathogens in neonatal septicemia and the antimicrobial resistance of these bacteria in recent years to guide the clinical treatment.METHODS The data of 76 neonatal septicemia confirmed by hemoculture from Jan 2005 to Jan 2008 in our hospital were analyzed retrospectively.RESULTS Coagulase-negative Staphylococcus(CNS) were the main pathogens,among which their were 47.36% of S.epidermidis,10.53% of S.haemolyticus,9.21% of S.aureus,and the others of 7.88% Escherichia coli.Bacteria were highly resistant to penicillin,ampicillin and erythromycin.Vancomycin,imipenem,ciprofloxacin and amikacin were the most sensitive drugs.CONCLUSIONS Gram-positive cocci are main pathogens in neonatal septicemia,in which the CNS are the most common ones.To choose sensitive antibiotics bases on the drug sensitivity tests may decrease the occurrence of drug resistance to bacteria and increase the clinical curative effects.

Objective To explore the incidence of olfactory dysfunction in patients with Parkinson disease and the characteristic as well as its possible influencing factors. Methods The SS-12 was used to evaluate the olfactory function of 106 patients with Parkinson's disease and 110 healthy volunteers. The data was then compared between the two groups to investigate the correlation of olfactory function with age, gender, education, smoking, disease duration, Hohn-Yahr stage, UPDRSⅢscores, the dosage of levodopao and olfactory scores. Results Mean identification scores were significantly lower in patients(5.97 ± 2.27)than in controls(8.04 ± 2.00)(t=7.108, P=7.108). Parkinson's disease group did worse than the control group in identifying some odors including peppermint, bananas,liquorice,coffee,pineap?ple,rose and fish (P<0.05). Receiver Operating Characteristic curves revealed that 7.5 was the best olfactory dysfunc?tion diagnostic boundary value with 67.3%sensitivity and 73.6%specificity. Thus, the incidence of olfactory dysfunc?tion in Parkinson's disease was 73.6%. Correlation analysis showed that gender(rs=0.243, P=0.012), education(rs=0.208, P=0.032) and smoking(rs=0.279, P=0.279) were associated with odor identification ability, while age, disease du?ration, Hohn-Yahr stage, UPDRSⅢscores and the dosage of levodopa were not related with odor identification abilityin Parkinson disease (P>0.05). Conclusion Olfactory dysfunction occurs in Parkinson disease with an hign incidence rate. Olfactory function has nothing to do with disease duration, Hohn-Yahr stage, UPDRSⅢscores and the dosage of levodopa in Parkinson disease.

Objective To establish and identify transgenic mice model that specifically express delta drosophila homolog-like 1 (DLK1) in liver for the functional study of DLK1 on liver differentiation,hepatopathy,and hepatocellular carcinoma.Methods The coding sequence of DLK1 cDNA was inserted downstream of mouse albumin gene enhancer/promoter to construct a liver-specific DLK1 expression recombinant vector.The DNA fragment of transgene digested from the recombinant vector by Pine I was transfected to Hepl-6 cells to verify the expression of DLK1 in vitro.Then the transgene fragment was microinjeeted into fertilized eggs of C57×CBA F1 mice and 20 transgenic founder mice were generated.The F1 mice of DLK1 transgenie founder mice were used to identify the expression of the transgene in liver and other tissues.Results RT-PCR and cellular immunofluorescence showed DLK1 expression when the transgene fragment was transfeeted into Hep1-6.For transgenic mice,RT-PCR and immunohistochemistry showed that DLK1 was specifically expressed in adult F1 mice liver.Conclusion Successfully established the liver-specific DLK1 expression transgenic mice model.

Hyperuricemia is closely associated with abdominal obesity .The prevalence of hyperuricemia combined with abdominal obesity has been increased significantly in recent years , along with the improvement of daily life and the changes in dietary structure .The state of hyperuricemia combined with abdominal obesity is most harmful , and becomes a common and high risk metabolic disease .Animal model with hyperuricemia combined with abdominal obesity is very impor-tant for the research of pathomechanism and treatment of this disease .

Objective To study the important virulence regulation genes of Brucella,and to understand their function.Methods Quantitative RT-PCR was used to quantify their relative transcription profiles under stress conditions and during macrophage cell infection.Results These genes were activated at different levels under these conditions and during cell infection,indicating their roles in pathogenesis at different srage of infection.Conclusion The transcription profiles of these genes have different effects about their functions.

Tremor, a rhythmic and involuntary oscillatory movement of one or several body parts, is the movement resulting from the abnormal synchronization of motor neural units. Detecting and analyzing the ACC, EMG and EEG signals of tremor patients by signal processing methods are very important for clinical diagnosis, rating evaluation and detection of incipient illness. This paper introduces the applications of time domain,frequency domain, artificial neural network, high order accumulation, approximate entropy, fuzzy, chaos, discriminant analysis in the researches of tremor signals, and finally points out the application foreground of researches on tremor signals.
Electroencephalography ; methods ; Electromyography ; methods ; Humans ; Motor Cortex ; physiopathology ; Muscle, Skeletal ; physiopathology ; Signal Processing, Computer-Assisted ; Tremor ; physiopathology

Objective: To explore the effect of mouth muscle training game on salivation cerebral palsy children. Methods: Sixty-eight children with cerebral palsy combined with salivation were randomly divided into experimental group and control group, with 34 cases in each group. The control group received routine rehabilitation treatment, while the experimental group performed oral muscle training game on the basis of the control group. Both the two groups were assessed by teacher salivation grading (TDS) and oral motor function assessment grading standards before and 8 weeks after intervention for salivation and oral motor function of cerebral palsy children. Results: After 8 weeks of intervention, there were 30 children with grade III-V salivation turned to grade I-IV in the experimental group, and 21 children with grade III-V salivation turned to grade I-IV in the control group, with the total effective rate of TDS of 88.2%(30/34) and 61.8%(21/34), respectively, and there was statistically difference (χ²= 5.02, P<0.05). The score of oral transport function in the experimental group was 87.68±12.93, which was higher than that of 76.53±10.92 in the control group, and there was statistically differences (t=3.841, P<0.01). Conclusions Oral muscle training games can stimulate self-exploration and training for oral movement of cerebral palsy children, so as to improve the range, flexibility and strength of the jaw, lip and tongue movement, and promote the self-control of swallowing of saliva, reduce the salivation and improve the quality of life.

Objective To explore the relationship of vertebral artery hypoplasia (VAH) with acute posterior circulation cerebral infarction (APCCI).Methods One hundred and eighty-nine consecutive patients diagnosed as having acute cerebral infarction admitted to our hospital from March 2015 to March 2016 were enrolled in our study;retrospective analysis was performed on their clinical and radiographic data to compare the general clinical characteristics of patients with posterior circulation infarction (PCI) and anterior circulation infarction (ACI).VAH was conformed by cranial magnetic resonance angiography (MRA),vertebral artery ultrasound or cranial MRA+vertebral artery Ultrasound.The infarction lesions in VAH patients with APCCI were typed;the correlation between vertebral artery diameter's differences and National Institute of Health stroke scale (NIHSS) scores in VAH patients with APCCI was illuminated.Results In 189 consecutive patients with acute cerebral infarction,32 (16.93％) were with VAH;after exclusion of 8 with infarction in both anterior and posterior circulation,90 (49.72％) were in PCI group while 91 (50.28％) in the ACI group.Univariate analysis showed that statistical differences were noted between the two groups in triglyceride (TG) and VAH levels (P＜0.05).In 23 patients with APCCI with VAH,brain stem infarction consisted of 9 patients (5 VAHs were ipsilateral to the infarction,3 VAHs contralateral and one bi-VAH),cerebellum infarction consisted of one patient (ipsilateral side),thalamus infarction 10 patients (4 VAHs were ipsilateral to the infarction,and 6 VAHs contralateral),occipital lobe infarction 2 patients (one ipsilateral and one contralateral) and cerebellum with thalamus infarction one patient (the side of VAH was ipsilateral to the cerebellum and contralateral to the thalamus).After excluding one patient with bi-VAH,in 22 patients with APCCI combined with VAH,significantly positive correlation between vertebral artery diameter's differences and NIHSS scores was noted (r=0.560,P=0.000).Conclusions It might be the best assessment standard of VAH measured by combination of transcranial MRA and vertebral ultrasound.TG and VAH may be risk factors of APCCI,and VAH could be one independent risk factor of APCCI.The side of VAH is usually ipsilateral to the brain stem and cerebellum infarction,while contralateral to the thalamus infarction in APCCI patients with VAH.Also,vertebral artery diameter's differences are positively correlated with NIHSS scores.

Objective:To explore the effect of International Classification of Functioning, Disability and Health, Children and Youth Version (ICF-CY) framed oral motor therapy combined with acupoint massage intervention on children with cerebral palsy swallowing disorder.Methods:From September 2018 to September 2019, 80 children with cerebral palsy accompanied by dysphagia who were diagnosed and treated in the Department of Neurological Rehabilitation of our hospital were selected as the study subjects, and were divided into the experimental group (40 cases) and the control group (40 cases) by random number table method. A total of 76 patients completed the study. On the basis of routine swallowing training and oral movement intervention, the experimental group was comprehensively evaluated from the aspects of body function and structure, activity and participation, and environmental factors with ICF-CY theory as the framework, and was given oral and facial acupoint massage for 12 weeks. The control group was given conventional swallowing training and oral exercise intervention for 12 weeks. The modified water swallow test was used to evaluate the effect of improving swallowing disorder in two groups of children after 12 weeks of intervention. The improvement of oral motor function in two groups of children after 4 and 12 weeks of intervention was evaluated by using the Grading Criteria for Evaluation of Oral Motor Function.Results:After 12 weeks of intervention, the total effective rate of swallowing disorder improvement in the experimental group and the control group were 89.47% (34/38) and 63.16% (24/38), respectively, and the difference between the two groups was statistically significant ( χ2 value was 7.28, P<0.05). After 4 and 12 weeks of intervention, the functional scores of oral motors in the experimental group were (67.13±2.35) and (82.08±2.33) points, respectively, which were higher than those of the control group at the same time (56.16±2.57) and (65.63±2.54) points, and the differences were statistically significant ( t values were -3.320, -4.433 P<0.05). After 4 and 12 weeks of intervention, the children's functional scores of oral motors in each group were higher than those in each group before intervention. Compared with the group before intervention, t values of the experimental group were -5.377 and -5.376; t values of the control group were -10.715 and -17.486; compared to the group with 4-week intervention, t value of the experimental group was -12.732; t value of the control group was -13.281, all the differences were statistically significant (P<0.05). The results of repeated measures analysis of variance showed that the time effect, grouping effect, and the interaction between time effect and grouping effect of functional scores of oral motors were statistically significant ( F values were 426.780, 9.084, 26.538, P < 0.05). Conclusions:Oral motor therapy combined with acupoint massage based on ICF-CY framework can effectively promotes the recovery and reconstruction of mouth perception and motor function in children with cerebral palsy, significantly improves the children's dysphagia and their ability to actively participate in feeding.