0.05). Conclusions The LTs synthesis is elevated in RSV bronchiolitis and LTs may participate in the pathogenesis of RSV bronchiolitis. Leukotriene receptor antagonist may play an important role in RSV bronchiolitis. However there still needs further research on the relationship between LTs and eosinophils count in the pathogenesis of RSV bronchiolitis.

Objective: To develop a novel fast auto blood viscosity analyzer based on the relation between flow rate and Casson viscosity of non-Newton fluid such as blood. Methods: Different from the tradition friction method of measuring blood viscosi-ty, the instrument was developed based on the Casson flow equation for blood in round tube and Stokes equation, using virtual instrument technique to calculate the Casson viscosity and stress, and pressure sensor and peristaltic pump to perform a setup. Results: Controlled by the data acquisition system, the blood Casson viscosity and stress were measured quickly and simply. Conclusions: Compared with the similar instruments used in clinic, this analyzer is faster in measurement and has high preci-sion, can determine the apparent viscosity and other Casson parameters of blood at any shear rate, and gives more clinic informa-tion for patients.

Objective To develop a method for the determination of cobalt in urine by graphite furnace atomic absorption spectrometry(GFAAS).Methods Usingthe matrixmodifier tofind the best condition byimprovingthe cineration temperature and reducing the interference of gas phase in atomization stage.Results The best cineration temperature and atomization temperature was 1 200 ℃and 2 450 ℃respectively,taking15% PdCl2,NH3H2PO4 and HNO3 as the matrix modifier.The background absorb value was less than 0.05.The linear range was 0～75 ?g/L,the correlation coefficient was over 0.999,the average recovery rate was 97.2%,the relative standard deviations were 3.5%～6.5%,the detection limit was 1.5 ?g/L.The result of 16 urine samples determined with the present method showed no significant difference compared with that by using polarography.Conclusion This method is sensitive,accurate,simple and is applicable tothe determination ofcobalt in urine.

Objective To investigate the causes and treatment of recurrent laryngeal nerve (RLN) injury during the operation of thyroidectomy. MethodsClinical data of 48 patients that RLN were injured during thyroidectomy in and out of our hospital from Jun. 2003 to Mar. 2007 were reviewed. ResultsNo patient died while operation and staying in hospital. There were 47 cases of unilateral RLN injury, 1 case of bilateral RLN injury; 21 cases (43.7%) were injured because of suture or scar adhesion, 13 cases (27.1%) were partly broken with formed scar, 14 cases (29.2%) were completely cut off; The locations of RLN injuries were closely adjacent to the crossing of the inferior thyroid artery and RLN in 13 cases (27.1%) and 35 cases (72.9%) were within 2 cm below the point of RLN entering into throat. The injured RLN were repaired surgically in 43 cases, among which 39 cases’ phonation and vocal cord movement were restored completely or had their vocal cord movement recovered partly; There were only 4 cases that the phonation and vocal cord movement were not recovered. Another 5 cases that did not take any repair did not recovered naturally. ConclusionThe location of most RLN injuries caused by mechanical injury during thyroid surgery is closely adjacent to the entrance of RLN into throat. Early nerve exploratory operation should be performed once the RLN is injured, and the method of repair should be decided according to concrete conditions of injury.

Objective To establish a SPE-HPLC method for the determination of carbaryl and atrazine in water.Methods Carbaryl and atrazine in water were extracted with Oasis HLB and were eluted with CH3OH,CH3OH-H2O(55:45) was applied as mobile phase,the flow rate was 0.8 ml/min,detection wavelength was 220 nm,column was Symmetry Shield RP18(150 mm ? 3.9 mm,5 ?m),carbaryl and atrazine in water were determined by PDA detector.Results The linear range of the method was 0.05-10.0 mg/L(y=316 263x-350 9,r=0.999 9,for carbary;y=140 151x-258 6,r=0.999 9,for atrazine),The lowest detected concentration was 0.0002 mg/L for carbary and 0.000 5 mg/L for atrazine,respectively(S/N≥3).The recovery rates were 89.6%-97.0%,and the relative standard deviation was 4.0%-6.7%.Conclusion The method was sensitive,accurate and repeatable,which was applicable to the determination of carbaryl and atrazine in water.

The formation of carcinomas of liver, lung and kidney in rats by feeding aminopyrine (AP) plus sodium nitrite (Na NO2) in diet was investigated. 72 young Wister rats were divided into A,B,C,D group. Group A was set as control receiving basal diet and tap water. Another 3 groups were given basal diet containing AP plus Na NO2 (2g/kg each). However, group B was given tap water, group C ascorbic acid solution, group D sea buckthorn (Hippophae rhamnoides L.) juice for drinking. After 38 week's feeding, group B developed tumors in the livers (17/17), lungs (6/17) and kidneys (4/17) and the average life-span was 195 days, group D delayed the development of tumors and the average life-span was 270 days, significantly longer than that of group B (195 days, p

Using calmodulin dependent cyclic neucleotide phosphodiesterase and dansyl-labeled calmodulin,honokiol's action on calmodulin is studied. It is found that honokiol inhibits the activity of cyclic neucleotide phosphodiesterase induced by stimulus of calmodulin. With the increasing of calmodulin concentration, the IC50 value was increased. In the presence of Ca2+, honokiol reduces the fluorescence intensity of dansyl-calmodulin, and makes the spectrumpeaked red. The results indicate that honokiol can combine with calmodulin in the presence of Ca2+ and antagonise the effect caused by calmodulin's stimulation upon the phosphodiesterase. In addition , honokiol may exert its effect on the basal activity of calmodulin dependent cyclic neucleotide phosphodiesterase.

Objective: To observe the therapeutic effects of Erchen decoction, Taohongsiwu decoction on CYP2E1 activity and microsome protein content in non-alcoholic fatty liver rats.Methods: Experimental animal model of non-alcoholic fatty liver rats were established , then treated with Erchen decoction, TaohongSiwu decoction.Microsome protein content and cyp2e1 activity are tested after treatment.Results: Microsome protein content of non-alcoholic fatty liver rats reduced and cyp2e1 activity increased. Compared with model group, in Erchen decoction group, the microsome protein content in hepatocyte increased significantly (P

The potential advantage of Traditional Chinese Medicine(TCM) theories of disease prevention,especial the thinking mode of "health preservation from both physique and spirit "has been generally recognized and widely understood by international society increasingly.In TCM,methods of disease prevention are various and the "health preservation from both physique and spirit" is praised the highest.This article demonstrates the theory that preserving the spirit firstly and then combining with physique,also the methods of keeping an undisturbed spirit and protecting the physique from any harm,for the purpose to make new contribution to the mankind’s health-care industry.

AIM:To approach the effects of Bacille Calmette-Guerin(BCG)on immunological function of infants with bronchiolitis in vitro. METHODS: The monocytes were segregated from the peripheral blood of infants with acute bronchiolitis and healthy infants as control. The cytokines of IL-10 and IL-12 were measured by ELISA after interfered with different concentrations of BCG in vitro. IL-4, produced by lymphocytes treated with the cultural supernatant of BCG-stimulated monocytes were determined by ELISA. Acute bronchiolitis infants and health children treated with normal saline were as control. RESULTS: BCG stimulated IL-10 and IL-12 production in a dose response manner in a certain concentration range. The higher level of IL-10 was observed when BCG concentration reached 50 ?L/mL, while IL-12 had no significant increase. The level of IL-4 was markedly decreased compared with pre-treatment (P