1.Chronic Gastritis (Liver Stagnation and Spleen Deficiency Syndrome) 45 Cases Treated by Chaishao Liujunzi Decoction
Journal of Zhejiang Chinese Medical University 2006;0(03):-
[Objective] To explore the curative effect of Chaishao Liujunzi Decoction for Chronic Gastritis(Liver Stagnation and Spleen Deficiency Syndrome).[Method] 90 cases were allocated to 2 groups(each group 45 cases) randomly.The treatment group was treated by the Decoction,the control group by Motilium and NexiumTablets.2 weeks constituted the course of treatment.The curative effect,improvement of symptoms and signs,manifestation of endoscopy before and after treatment were observed.[Result] The curative effect in treatment group was significantly different as compared with control group after treatment(P
2.Application progress of 18F-FDG positron emission tomograph in differentiated thyroid cancer.
Journal of Biomedical Engineering 2014;31(2):445-451
Positron emission tomography (PET) is a highly sensitive and low invasive technology for cancer biological imaging. Integrated PET/computed tomography (PET/CT) cameras combine functional and anatomical information in a synergistic manner that improves diagnostic interpretation. The role of 18F FDG PET/CT in differentiated thyroid cancer (DTC) is well established, particularly in patients presenting with elevated thyroglobulin (Tg) levels and negative radioactive iodine scan. This review presents the evidence supporting the use of 18F FDG PET/CT throughout the diagnosis and management of thyroid cancer, and provides suggestions for its clinical uses.
Fluorodeoxyglucose F18
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Humans
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Positron-Emission Tomography
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Thyroglobulin
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blood
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Thyroid Neoplasms
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diagnosis
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Tomography, X-Ray Computed
3.Correlation between microtubule damage and mitochondria damage in hypoxic cardiomyocytes
Journal of Third Military Medical University 2003;0(15):-
Objective To investigate the relationship between microtubule damage and mitochondria damage in hypoxic cardiomyocytes.Methods Cardiomyocytes from neonatal Wistar rat were isolated and cultured for 3-5 d,then divided into three groups: normal control,hypoxia(cultured in hermetic container containing 1%O_(2),5%CO_(2),95%N_(2)),hypoxia with microtubule depolymerization(by adding 4 ?mol/L colchicine).The fluorescence intensity of ?-microtubule was determined by flow cytometry,the morph and distribution of ?-microtubule and mitochondria were checked by confocal laser scanning microscopy(CLSM) at 30 min,1 h after the cadiomyocytes being oxygen deficit.Results As compared with the normal control cells,the fluorescence intensity of ?-microtubule decreased and the microtubule ruptured and distributed confusedly,and the mitochondria changed into disorder in hypoxic cells.As compared with the hypoxic cells,the distribution of mitochondria confused and the fluorescence intensity decreased in the cells of hypoxia with microtubule depolymerization.Conclusion Microtubule damage occurred at the early stage of hypoxia(30 min).Microtubule damage may lead to mitochondria damage in hypoxic cardiomyocytes.
5.Influence of Eryngium foetidum L. on Inflammatory Cell Factors of Pleuritics Rat Model
Ling HUANG ; Zaoyuan KUANG ; Zeying LIU
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(03):-
Pleuritis rat model was adopted to observe the influence of Eryngium foetidun L . on in- flammatory cell factors (TNF-a, IL- 6 and NO) . The results showed that Eryngium foetidum L. can decrease the contents of TNF-a , IL- 6 and NO in serum. It is indicated that the anti- inflam- matory effect of Eryngium foetidum L. is probably related to the decrease of the contents of the above factors.
6.MRI study on the hippocampal atrophy due to mild Alzheimer's disease
Weihong KUANG ; Mingsheng HUANG ; Jin LI
Chinese Journal of Geriatrics 2000;0(04):-
Objective To study the features of hippocampal atrophy (HA) by MRI in mild Alzheimer's disease(AD) patients. Methods Fifteen mild AD patients and 30 healthy controls were included in this study and all subjects were examined by both MRI and MMSE. The differences in the hippocampal volume between the mild AD and the control were analysed, and relationships between the hippocampal volume and age, MMSE scores were evaluated. Results The hippocampal volume of the mild AD was decreased significantly than that of the controls 〔(2 08?0 46)cm 3 vs (3 11?0 19)cm 3, t =2 71, P 0 05). Conclusions MRI-based HA could be used to differentiate the mild AD patients effectively from the healthy subjects and could also be regarded as a status marker for the early clinical diagnosis of AD.
7.Application of polyterafluoroethylene in repairing secondary nasal deformity of unilateral cleft lip after opration
Guangxiang HUANG ; Shifeng KUANG ; Guosan DENG
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(04):-
Objective To explore the methodology of repairing secondary nasal deformity of congenital unilateral cleft lip after operation and clinical outcome of the patients. Methods The repairing operation were performed in 46 cases,in which polyterafluoroethylene (PTFE) implants were inserted into a pocket under the nasal dorsum, ala or base through a small incision. Results The flat nasal dorsa, tips and hollow nosal base were elevated. The profile view of noses was improved apparently. The outcome was satisfactory. Conclusion PTFE is a optimal plastic material,which is compatible perfectly with circumferential tissues. The outcome of filling is excellent. Therefore, this technique is one of the efficient methods to repair secondarynasal deformity of unilateral cleft lip.
8.Treatment of Parkinson's Disease by Microelectrode-guided Thalamotomy and/or Pallidotomy: a Report of 225 Cases
Qichang ZENG ; Hongxing HUANG ; Weiping KUANG
Journal of Chinese Physician 2001;0(07):-
Objective To explore indications, ablated targets, efficacy and complications of microelectrode-guided thalamotomy and pallidotomy for treating Parkinson's disease(PD). Methods The clinical date of 225 PD patients underwent microelectrode guided thalamotomy and/or pallidotomg were retrospectively analyzed with UPDRS scores. Results The outcome of operation was excellent.The rate of improvement for tremor, rigidity, gait, balance and bradykinesia were 98 0%, 89 0%, 72 8%, 70 5% and 47 8% respectively. Postoperative motor UPDRS scores of both "on"/"off"states significantly decreased by 54 4% and 62 6% respectively. The frequency of post-operative complications was such as the following: bleeding caused by puncturing (0 48%),transient hiccup(6 7%), temporary dysarthria(5 5%), transient somnolence(10 0%) and mental disorder(4 5%). There were no severe and permanent complications. Conclusions The thalamotomy and/or pallidotomy guided by the microelectrode were the effective and safe method for treatment of PD, but the indications of this surgery should be well familiarize. The ablated target selection and its precise localization were of key importance for a better outcome.
9.Relationship between hemolymph phenol oxidase and melanization of Plasmodium yoelii oocysts in Anopheles dirus
Wenyue XU ; Fusheng HUANG ; Xilin ZHANG ; Mingshu KUANG ; Jianhua DUAN
Journal of Third Military Medical University 2001;23(4):440-442
Objective To explore the relationship between hemolymph phenol oxidase and the melanization of Plasmodium yoelii oocysts in Anopheles dirus. Methods An Anopheles dirus-Plasmodium yoelii system was used Anopheles dirus were divided into 3 groups, that is, non-blood-fedding (N), normal-blood-fedding (B) and infected-blood-fedding (I). The activities of MPO and o-DPO in hemolymph from 3 groups were determined with native polyacrylamide gel electrophoresis (PAGE) and density scanning at 5, 7, 11 and 15 d after blood feeding. Results Both MPO and o-DPO activity were significantly higher in group I than group N and B (P<0.05). But with the melanization of Plasmodium yoelii oocysts, both MPO and o-DPO activity in group I were decreased in comparison with group N, especially on the 15 th day after infected-blood feeding. MPO and o-DPO activity in group B were significantly stronger than those of group N. Conclusion Blood feeding and infection of Plasmodium yoelii both can activate the cascade. The heamolymph phenol oxidase may play an important role in the melanization of Plasmodium yoelii oocysts in Anopheles dirus.
10.Comparison of Total DNA Extraction Methods for Studying Endophyte Diversity in Mulberry
Yali HUANG ; Man MA ; Ren ZHANG ; Xing WAN ; Zaoyuan KUANG
Journal of Guangzhou University of Traditional Chinese Medicine 2015;(4):729-734
Objective To compare the advantages and disadvantages of the four DNA extraction methods according to the endophytic diversity in the roots, stems, and leaves of mulberry analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) , and by taking the yield, purity and PCR amplification as indexes. Methods Four common methods, i.e., cetyltrimethyl ammonium bromide ( CTAB) , sterile phosphate buffered saline (SPBS) vibration, liquid nitrogen grinding (LNG) , and KIT methods, were used to extract the total DNA from different tissues of mulberry, and then were compared based on the diversity analysis results for endophyte by PCR-DGGE. Results From the roots and stems of mulberry, we got the highest concentration of DNA by LNG extraction method, and got the lowest concentration by SPBS extraction method. But for the leaves of mulberry, the results of the four extraction methods were completely opposite to those for the roots and stems. For different tissues of mulberry, the purity of DNA extracted by KIT method was the best. According to the endophytic bacteria diversity analyzed by 16S rDNA PCR-DGGE, the appropriate method for extraction of DNA was LNG or CTAB, but was not KIT. And according to the results of endophytic fungi diversity analyzed by ITS PCR-DGGE, the best extraction method was KIT, and the unsuited methods varied from the tissues of mulberry. Conclusion The optimum DNA extraction method for mulberry varies from the tissues of mulberry and endophtic bacteria.