Objective:To establish a microfluidic chip system for immunoassay.Methods:We use direct-writing laser micromachining technology to fabricate microfluidic chip,establish microfluidic chemiluminescence immunoassay system based on superparamagnetic microbeads,and use this system to detect alpha-fetoprotein. Results:We can complete the analysis within 20 minutes with 5?l sample or reagent,the linear range was 1-800 ng/ml with average intra-assay and inter-assay precision at 6.3% and 7.8%,respectively and average recovery at 94.3%. Conclusion:The microfluidic chip system is a time-saving,cost-low and convenient method for AFP analysis.

Parpillary adenocarcinoma is the most common type of thyroid cancer with high probability of cervicel lymph node metastasis. Prophylactic neck dissection is not necessary for patients with clinical negative neck. Modified radical neck dissection is the standard for therapeutic neck dissection. The range of dissection remains controversial. Level Ⅰ is not included routinely, level Ⅱ is recommended. Dissection of level Ⅴ may cause malfunction of the neck and shoulder, clinical negative level Ⅴ for low-risk group can be avoided with close follow-up. Rountine central lymph node dissection tends to reduce the local recurrence. Selective neck dissection for thyroid parpillary adenocarcinoma needs more investigations.

Vascular calcification is a common pathology frequently occurred in the process of atherosclerosis ,hypertension , diabetes,chronic kidney disease,vascular injury and aging,resulting in an increased prevalence of cardiovascular events. Along with the social aging process,the incidence becomeshigher,andthis attract more attention. This review summarized a series of our works on vascular calcification in recent years ,including the update concept of vascular calcification ,recent classification and mechanism,and the confusion and hope in experimental and translational researches. This article aiming to promote further studies on the mechanisms and prevention of vascular calcification.

Objective To investigate the clinical effects and significance of continuous intravenous infusion and sequential therapy in pneumonia in children.Methods 90 patients with pneumonia were randomly divided into the observation group and the control group,with 45 patients in each group,observation group received continuous intravenous infusion,the control group received sequential therapy.The expectoration and rales disappear time,chest X-ray and body temperature return to normal time,clinical efficacy were observed.Results The expectoration and rales disappear time,chest X-ray and body temperature return to normal time were longer than control group,which were significantly different (x2 =6.21,P ＜ 0.05),the total effective rate of observation group was 77.8％,the total effective rate of control group was 97.8％,the differences were statistically significant (t =3.58,4.62,6.18,7.01,all P ＜ 0.05).Conclusion The sequential therapy treatment pneumonia in children has better clinical effect,shorter hospital stay and worthy of clinical application.

Objective To explore the relationship between the clinical characteristics and laboratory examinations.Methods Retrospective analysis was performed on the clinical data in 172 patients diagnosed as infectious mononucleosis(IM)in our department recently.Results The onset rate was high from 3 to7 years old.The main clinical presentations included fever,neck lymphadenopathy,pharyngitis,hepatomegaly,and splenomegaly.Some patients accompanied with obvious sonorous.The occurring rate of the complications was 74.4%,especially deteriorated liver function was the most common,next came to the lung infection.The proportion of atypical lymphocytes raised in 73.3% cases.The positive rate of EBV-VCA-IGM was 65.1%.Myocardium ferment chart went up in 27.9% cases,Erythrocyte sedimentation rate increased fast in 61.6%,CRP gently got higher in 62.8%.However,the ratio of atypical lymphocyte and EBV-VCA-IGM positive or negative had little to do with the pathologic condition and complications in IM.Conclusions The clinical progress of most IM is benign and multiple organs can be involved with atypical clinical manifestations.Examination for atypical lymphocytes is an easy and effective means to screen IM.

AIM:To approach the effects of Bacille Calmette-Guerin(BCG)on immunological function of infants with bronchiolitis in vitro. METHODS: The monocytes were segregated from the peripheral blood of infants with acute bronchiolitis and healthy infants as control. The cytokines of IL-10 and IL-12 were measured by ELISA after interfered with different concentrations of BCG in vitro. IL-4, produced by lymphocytes treated with the cultural supernatant of BCG-stimulated monocytes were determined by ELISA. Acute bronchiolitis infants and health children treated with normal saline were as control. RESULTS: BCG stimulated IL-10 and IL-12 production in a dose response manner in a certain concentration range. The higher level of IL-10 was observed when BCG concentration reached 50 ?L/mL, while IL-12 had no significant increase. The level of IL-4 was markedly decreased compared with pre-treatment (P

Glucokinase (GK) is considered to be the key regulatory enzyme in glucose metabolism, whose activity changing will lead to glucose metabolic disturbance and diabetes. Recently, people have realized that there is a good extensive perspective in diabetes research by means of glucokinase investigation. In this paper, we review the mechanism of diabetes resulting from abnormality of glucokinase activity and the status of glucokinase activators, propose the viewpoint that glucokinase is a powerful target for diabetes therapy and research, and illuminate that searching of glucokinase activators is the guidance in the future.

0.05).Conclusions These results suggest that the level of serum ECP can reflect the inflammation in the bronchial tube and the activated state of eosincyte more sensitively than IgE.

Objective: To explore the relationship between the cognitive style of field dependence-independence and self-supporting levels in primary school students.Methods: One hundred and twenty grade 4 to 6 primary school students were examined by the Self-supporting Behavior Questionnaire for 6 to 12 Years Old Children,and then the subjects were divided into two groups,of which the high-self-supporting group includes the top 27% according to the questionnaire total score,and the low-self-supporting group includes the bottom 27%.Both the groups were tested by the Embedded Figures Test.Results: ①The high self-supporting group get significantly higher score in the Embedded Figures Test than the low self-supporting group;and the field-independence group get significantly higher scores in the scholastic self-supporting and self-determination subscales of the Self-supporting Behavior Questionnaire than the field-dependence group;②the score of Embedded Figures Test was significantly correlated with the scores of scholastic self-supporting,self-supporting in daily life,self-determination,self-action,responsibility undertaking,and the total score of the Self-supporting Behavior Questionnaire.Conclusion: Children's self-supporting levels were related to their cognitive style,namely,the high self-supporting children were inclined to field-independence cognitive style,and vice versa.

In order to deal with huge work load of processing and reviewing applications,many personnels have been loaned from universities and research institutes to National Natural Science Foundation of China (NSFC) every year.This phenomenon has received much concern.In this paper,the features of NSFC loaned personnel model were analysed,and the necessity for and feasibility of improving the training function of NSFC by reforming current loaned personnel model were discussed.