Objective To observe the clinical effect and the incidence of neuralgia of acupoint injection with nerve growth factor onRamsay Hunt syndrome. Methods 65 patients with Ramsay Hunt syndrome were divided into treatment group (n=33) and control group(n=32). All the patients were treated with the anti-virus and glucocorticoid medicine, and were acupunctured at the similar points with electroacupuncturewith the disperse dense wave. The treatment group was injected with mouse nerve growth factor at the acupoints. 2 days offevery 5 times, 10 times as a course for 2 courses. The treatment effect and the incidence of sequelae neuralgia after 3 months were compared.Results The total effective rate of treatment group (93.9%) was significantly higher than control group (71.9%) (P<0.01). The incidenceof sequelae neuralgia of the treatment group (6.3%) was significantly lower than the control group (26.7%) (P<0.01). ConclusionAcupoint injection of mouse nerve growth factor can improve the efficacy and reduce the incidence rate of sequelae neuralgia in patientswith Ramsay Hunt syndrome.

Objective To study the application of waveforms in treatment of peripheral facial paralysis with electroacupuncture. Methods 66 patients with peripheral facial paralysis were divided into two groups: treatment group (34 cases) and control group(32 cases). All the patients were acupunctured at the similar points with electroacupuncture, while the disperse-dense wave and the discontinuous wave were used successively in the treatment group, and the disperse-dense wave was used alone in the control group. Results There were significant differences between the two groups in the total effective rate and the days for cure (P<0.01). Conclusion The effect of alternate disperse-dense wave and discontinuous wave is superior to that of disperse-dense wave alone in electroacupuncture treatment for peripheral facial paralysis.

Myoblast transplantation has been carried out for a certain period,but its efficiency in repairing inherited myopathy or severe muscular injury is not satisfactory.The improvements in myoblast culture,transplantation approach,recipient and cell preparation before transplantation,scaffold material and its selection,and body immunity can positively influence transplantation efficacy,and improve recipient function.In addition,these improvements influence the prospect of myoblast transplantation or cell-mediated gene therapy.

Objective To explore the size changes of erythrocytes infected with Plasmodium malariae,so as to improve the basic?level experimenters’microscopy capabilities for P. malariae identification in thin blood smears. Methods The micro?scopic features of erythrocytes infected with P. malariae in thin peripheral blood smears were observed,and a microscope image processing software was used to measure and analyze the diameter changes of the erythrocytes infected with P. malariae. Re?sults The diameter of erythrocytes infected with P. malariae decreased significantly compared with that of the normal erythro?cytes. The three parameters in this study:the diameter of erythrocytes,the value of diameter variation,and the ratio of diameter variation varied at different developmental stages of P. malariae,and the differences were statistically significant(all P<0.01). The variances of the three parameters grouped by different cases or different developmental stages in different cases were ana?lyzed,all showing statistically significant differences(all P<0.01). Conclusion All the developmental stages of P. malariae will cause the decrease of the diameters of infected erythrocytes in peripheral blood smears,but the influence on the diameter of erythrocytes,value of diameter variation,and ratio of diameter variation varies at different developmental stages in different cases.

Objective To compare the two kinds of the single nostril transsphenoidal approach of minimally invasive surgery of pituitary adenoma and analyze the clinical data ,and then provide references for clinical diagnosis and treatment .Methods The prospective study methods were used in the study .A total of 54 cases of pituitary tumor patients were collected , they were randomly divided into microscope group ( 31 cases ) and neural endoscopic group (23 cases).The operation time,intraoperative blood loss,hospital stay,the resection rate,postoperative complications ( mainly observed transient postoperative insipidus and transient electrolyte disorder ) , the hormone levels , and the comparison the results of patients ,follow-up within 1 year were observed .Results The operation time of microscope group(121.3+19.4)min was longer than that of the neuroendoscopic group (78.5 ±7.8)ml,but the intraoperative blood loss in the microscope group was less than that of the neuroendoscopic group [(50.3 ±3.2) mL vs (78.5 ± 7.8)mL,t=0.993,1.032,all P>0.05].The postoperative hospital stay [(8.7 +1.1) d vs (9.1 +2.3) d,t=2.897,P>0.05],the resection rate(93.5% vs 91.3%,χ2 =3.191,P>0.05) had no significant differences be-tween the two groups .The postoperative complications ( including transient insipidus and transient electrolyte disorder ) also had no obvious differences .The analysis of the endocrine hormone levels of different tumors found that there were no significant differences between the microscope group and neuroendoscopic group .The levels of these pre operative and postoperative hormones were as follows:PRL[(387.3 ±100.8)μg/L vs (145.3 ±27.4)μg/L and (390.2 ± 133.7)μg/L vs (148.4 ±57.2)μg/L],GH[(63.4 ±5.7)μg/L vs (10.6 ±2.4)μg/L and (65.6 ±6.2)μg/L vs (12.4 ±1.6)μg/L],ACTH[(202.9 ±73.7)ng/L vs (38.5 ±4.6)ng/L and (206.8 ±78.6)ng/L vs (35.6 ± 2.5)ng/L],they all had significant differences (t =1.456,1.301,0.973,1.034,0.774,0,732,all P <0.05). Conclusion The curative effects of the two surgical treatment of pituitary adenoma have no obvious differences ,they have advantages of minimally invasive , safe and effective, but the microscopic surgery has less bleeding , while neuroendoscopic surgery can shorten the operation time .

Polyamidoamine (PAMAM) dendrimers is considered a better genetic carrier for its ability to form with DNA a nano-sized polyelectrolyte complex of high stability and dissolubility. The mechanism of PAMAMmediated gene endocytosis has been fairly clear and the researches in vitro have confirmed that PAMAM had high transfection efficiency and low cytotoxicity. The researches in vivo have further confirmed that PAMAM had a potential application foreground in the field of gene therapy including in the prevention and treatment of ewing's sarcoma and pulmonary fibrosis, in heart transplantation, and in the preparation of falciparum malaria vaccine.

Objective To study the influence on xerostomia of radiotherapy in treatment of head and neck cancer.Methods 82 cases with head and neck cancer from August 2005 to January 2006 were analyzed retrospectively.All patients were asked about their xerostomia at the end of every week.Then xerostomia and xerostomia-related problems were analyzed.Results There were obvious difference in the incidence rate of xerostomia( ?2=26.24,P 0.05).Conclusion During radiotherapy,acute radiation xerostomia may happen.The rate of it is different.depending on the radiation dose to be accepted while not on the age.

Pesticides plays an important role in the history of agricultural development,but the health hazards and environmental pollution problems are attracting increasing attention. Methyl parathion is one of the most widely used pesticides,its threat to human health caused increased emphasis. This paper introduced the chronic toxicity of methyl parathion to various systems such as the nervous system,endocrine system,immune system and reproductive system.

Objective To observe the effects of esophageal stricture treated with esophageal stenting.Methods 23 esophagus stenosis patients were expanded to 1 lmm,and the stent was implanted-.Results 23 patients were set into successfully,7 cases could enter the common food,11 caces could enter the soft food,5 cases could enter the semiliquid food.18 cases had the ache,6 cases had the stomach esophagus regurgitation symptom.During 1?12 month follow-up,10 cases survived,13 cases died,the cause of death was mainly later period cancer failure and metastasis.Conclusion Esophagus stenosis treatment operation was simple,safe,and the wound small,the short-term curative effect would be good.

Objective:To investigate procalcitonin and C-reactive protein levels in diarrheal patients who underwent irinotecan che-motherapy. Methods:Procalcitonin and C-reactive protein were detected among 85 diarrheal and 63 non-diarrheal patients after irinote-can chemotherapy. Results:According to WHO classification, patients without diarrhea are classified as grade 0, whereas patients with diarrhea can be classified as gradesⅠ-Ⅳ. In grades 0,Ⅰ,Ⅱ,Ⅲ, andⅣpatients, the levels of procalcitonin were 0.29 ± 0.17, 0.30 ± 0.18, 0.36 ± 0.20, 1.24 ± 0.22, and 2.15 ± 0.26 ng/mL on the second day, respectively. However, on the fourth day, the procalcitonin lev-els were 0.28 ± 0.15, 0.30 ± 0.14, 0.34 ± 0.18, 2.00 ± 0.22, and 2.40 ± 0.28 ng/mL, respectively. Moreover, in grades 0,Ⅰ,Ⅱ,Ⅲ, andⅣ, the levels of C-reactive protein were 6.06 ± 1.85, 6.12 ± 1.16, 6.20 ± 1.68, 22.62 ± 4.55, and 31.26 ± 5.23 mg/L on the second day, respectively. On the fourth day, the C-reactive protein levels were 5.80 ± 1.82, 5.94 ± 1.14, 6.15 ± 1.55, 30.52 ± 4.74, and 38.67 ± 5.68 mg/L, respectively. No significant difference was found between the procalcitonin and C-reactive protein levels of stagesⅠandⅡpa-tients (P>0.05), but a significant difference was found between stagesⅠ, andⅡpatients and stagesⅢandⅣpatients (P<0.05). Con-clusion: Monitoring levels of procalcitonin and C-reactive protein may be helpful in the early evaluation of the severity of diarrhea. This process has prognostic effect and can be used to assess whether patients have enterogenous bacterial infection. Monitoring the lev-els of these proteins has certain clinical value and can be used to guide early anti-infection therapy.