China ; Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening

Calreticulin (CRT) participates in the progression and apoptosis of many kinds of tumor cells. Recent studies indicate expression of CRT on tumor cells acts as an "eat me" signal for dendritic cells,allowing them to phagocytose tumor cells. It can induce immunogenic tumor cell death by adsorbing recombinant calreticulin to tumor cells or by enforcing the translocation of endogenous calreticulin to the cell surface. In animal experiments,CRT related tumor vaccine has good therapeutic effects in some tumor bearing mice,which can be used in tumor immunotherapy and targeted therapy.

Objective To clarify the morphological features, types, regional distributions and cell densities of gastrointestinal (GI) endocrine cells in various parts of the digestive track (DT) of Eumeces elegans. Methods Using immunohistochemical techniques of streptavidin-peroxidase(S-P) method. Results The endocrine cells found in the DT were round or oval, spindle, shuttle, pyramid, flask and bacilliform in shape. Three types of endocrine cells, namely 5-hydroxytryptamine (5-HT) positive cells, somatostatin (SS) positive cells and gastrin (GAS) positive cells were found. No substance P(SP)-, glucagon (GLU)-, pancreatic polypeptide ( PP)- and insulin(INS) positive cells were detected. 5-HT positive cells distributed throughout the whole DT at various densities and they were most predominant cell types in the DT. SS positive cells were detected only in stomach. GAS positive cells showed a restricted distribution and were only demonstrated in the pylorus and duodenum. In the DT of E. elegans the region with the highest degree of cell type heterogeneity was pylorus and all types of endocrine cell along the DT showed peak density in the pylorus as well. Conclusion Some common features of the distribution of different types of GI endocrine cells are found between E. elegans and other reptiles. This common trait may reflect the similarity in digestive physiology of various vertebrates. On the other hand, some species-dependent unique distribution features of endocrine cells in DT were also detected in E. elegans.

Objective To study the effects of repetitive transcranial magnetic stimulation (rTMS) on rats'locomotor function after hemisectional spinal cord injury (SCI). Methods A model of hemisectional SCI was established at the T10 segment in 12 rats,which were then randomly divided into an SCI-rTMS group and an SCI-control group.Another 6 rats served as normal controls.The SCI-rTMS group received threshold rTMS daily for 4 weeks,while the SCI-control group was given sham rTMS.The normal control group was left without any treatment.BassoBeatti-Bresnahan (BBB) locomotor scores were recorded weekly.Motor evoked potential (MEP) was detected at the 28th day after the operation.Neurofilament-200 (NF-200) was detected with immunofluorescent staining of the spinal cord lesions. Results The BBB scores in the SCI-rTMS group improved more than those in the SCI-control group.MEP of the fight hindlimb was detected in the rTMS group,but not in the control group.Expression of NF-200 marker increased significantly more than in the rTMS group. Conclusions rTMS can improve locomotor function of rats after spinal cord hemisectioning,which may result from increased expression of NF-200 and the regeneration of axons.

A patient with sparganosis mansoni was treated with praziquantel and cured with little side effect.

A patient with eosinophilic syndrome due to Paragonimus infection wag treated with praziquantel and cured.

Objective To investigate the activation of matrix metalloproteinase (MMP)9 by acute arterial injury and the involved signaling mechanism in murine femoral artery. Methods In the C57BL/6 mice femoral artery denudation injury were performed. Total protein and membrane protein extracts were prepared from targeted arteries. The MMP 9 activity was measured by zymography assay, the expressions of MMP 9 antigen and protein kinase C (PKC) isoforms were measured by Western blot. Seventy-two hours after mice fed with PKCβ inhibitor (ruboxistaurin), the denudation injury triggered MMP 9 activation was reassessed. Results Within 4-24 hours after denudation injury, MMP 9 activity in femoral arteries was significantly increased, with a peak induction of (99.3±9.5) times the sham control (F=51.49,P＜0.01) at 8 h. MMP 9 antigen increased in parallel with MMP 9 activity. Within 15-120 minutes after denudation injury, there was a significant induction of PKCβⅡ in membrane fraction of femoral arteries, with a maximum induction of (7.50±0.60) times the sham control (F=207.06,P＜0.01)at 30 min. Injury-induced MMP 9 activation was significantly inhibited by ruboxistaurin. Conclusions MMP 9 activation is, at least in part, mediated by PKCβ in acute arterial denudation injury, it highlights the new target for therapeutic intervention to suppress the over-activation of MMP 9, which plays a critical role in restenosis.

AIM:To observe the effect of supplementing bilirubin on the anti-oxidative stress ability of red cell membrane in training rats through establishing animal models of high-intensity training. METHODS:The experiment was performed in the physiological laboratory of Physical Institute of Jiangxi Normal University from December 2004 to January 2005.①After one week of adaptive feeding,24 male SD rats were randomly divided into quiet control group,high intensity sports training and physiological saline group(training group),high intensity sports training and bilirubin group(training and supplementing group)with 8 animals in each group.②The training group and the training and supplementing group did the running on the platform with the slope of 0?at the first three weeks and 5?at the fourth week,6 days every week expect Sunday.The training and supplementing group was intragastrically infused with bilirubin by 40mg/kg body mass at 17:00-18:00 after training every day,and the other groups were given physiological saline,six times every week.Anticoagulatory blood about 2mL of heparin was harvested to prepare red cell membrane sample and measure the relevant indexes on the 4~(th)Sunday.③The serum total bilirubin level was determined using automatic biochemical analysis instrument;the malondialdehyde(MDA)and glutathione peroxidase(GSH-Px)of red cell membrane by TBA;the activity of superoxide dismutase(SOD)of red cell membrane using the method of Xanthine oxidase.All the treatment for animals was based on the ethical standards. RESULTS:All 24 rats were involved in the result analysis.①The serum total bilirubin level in training group was lower than quiet control group(P0.05).②The MDA of the red cell membrane in the training group was higher than the control and training and supplementing group(P0.05).③The activity of SOD in training group was lower than quiet control group(P0.05),but the training and supplementing group was higher than the control group and the training group(P

Objective To detect the expression of HSPA2 mRNA and protein in pancreatic cancer and corresponding adjacent nontumorous tissues, and investigate the expression of HSPA2 protein in pancreatic cancer and its association with clinicopathological characteristics.Methods Western blot and qPCR (real-time quantitative PCR) was used to evaluate HSPA2 expression in pancreatic cancer and corresponding adjacent nontumorous tissues.Immunohistochemistry was used to investigate the HSPA2 expression in pancreatic cancer and its association with clinicopathological characteristics.Results Both the mRNA and protein levels of HSPA2 were significant higher in pancreatic cancer tissues than those in their paired adjacent nontumorous tissues.High HSPA2 expression was detected in 68.8% (55/80) of pancreatic cancer tissues.HSPA2 protein expression was significantly associated with tumor differentiation, vascular invasion, and status of metastasis (P=0.011,0.005,0.012).Conclusion HSPA2 expression is elevated in pancreatic cancer, and positively correlated the process of invasion, metastasis and progression of pancreatic cancer.

Objective To investigate the clinical effect of Sodium Hyaluronate Injection and psychological intervention in the treatment of knee osteoarthritis. Methods The control group of patients with knee osteoarthritis treated treatment group in the conventional research on Sodium Hyaluronate Injection, Sodium Hyaluronate Injection treatment combined with psychological intervention, record the two groups before and after treatment of knee joint function changes. Results There was no significant difference between the two groups in the knee function before treatment. After the corresponding treatment, the Lysholm scores of the two groups were significantly higher than before, and the knee joint function improvement in the study group was better (P<0.05). Conclusion The application of sodium hyaluronate and psychological intervention in the treatment of osteoarthritis of knee joint can significantly improve the efficacy of patients, and is conducive to the protection of their physical and mental health, quality of life.