Objective To observe the influences on NO, NOS, ET of the Therapy of Tonifying the Kidney in patients with angina pectoris. Methods 50 patients with angina pectoris were treated by Chinese medicine of tonifying the kindey and the NO, NOS, ET, symptoms, ECG (ischemic ST-T changes), holter ECG, blood viscosity and blood fat were recorded. Results Obvious rise of NO, NOS and drop of ET. The improving of NO(?mol/L) from 46.42?7.94 to 70.16?12.94, NOS(u/mL) from 1.62?0.22 to 2.36?0.30, ET(pg/mL) from 64.46?15.12 to 41.42?8.76, the total efficacy of Chinese medicine of tomifying the kidney to improving of angina pectoris amounted to 92%, to improving of ECG amounted to 68%, the improving time (minutes) of holter ECG from 104.70?12.25 to 24.26?3.41. The rise of NO, NOS, the drop of ET and the therapeutic efficacy of Chinese medicine of tonifying the kidney are better than isosorbidi mononitratis with significant difference (P

Objective To observe the effect of the therapy of tonifying the kidney on plasma NO and ET in dog with myocardial ischemia. Methods 20 dogs of myocarclial ischemia were divided into the model group control group, isosorbide mononitrate group and the therapy of tonifying the kidney group by random. The NO and ET of serum were measured before and after treatment. Results The level of NO was higher and the level of ET was lower than that of the isosorbide mononitrate group. There was significant difference between the control group and the therapy of tonifying the kidney group (P

Objective To analyse CT signs of lumbar spinal degenerative diseases.Methods CT findings of lumbar spine in 1180 cases with degenerative disease lumbocrural pain were analysed.CT classification of degenerative disease of lumbar spine was carried out according to the lumbar structures in combination with clinical sign.Results Lumbar spinal degenerative disease could be classified into 6 types:intervertebral disc degeneration(degenerative rate 65.3%);lumbar vertebra degeneration(degenerative rate 48.1%);vertebra facet joint degeneration(degenerative rate 36.0%);ligment degeneration(degenerative rate 25.4%) ;degenerative stenosis of lumbar spinal canal(degenerative rate 44.0%);and degenerative lumbar spine unstability(degenerative rate 25.0%).The former 4 types were belonged to primary degeneration of lumbar spine,the latter 2 types were belonged to secondary degeneration of lumbar spine.Conclusion CT classification of lumbar spinal degenerative disease is helpful to clinic in diagnosis and therapy.

Objective To study the Value of low dose CT scanning with gas-contrasted and low window level in diagnosing gastro-colon lesions.Methods 126 cases suspected with gastro-colon lesions were randomly devided into two groups,group A(69 cases) were scanned with conventional water-contrast or non-contrast and conventional expose dose,and with conventional window level,group B(57 cases) were scanned with gas-contrasted and low expose dose,and using low window level.The images were evaluated by 2 experienced radiologists,the results including doctor's self-confidence and diagnostic accurate rate in both group A and group B were compared based on the endoscopic results.Results The diagnostic self-confidence of radiologists and the accurate rate in evaluating the lesions in group B were obviously superior to group A.Conclusion CT scanning of gastro-colon with gas-contrasted,low expose dose and low window level is markedly superior to conventional one.

Objective To design capsule of gas-produced powder.Methods Glutin capsule was prepared with 0.50g gas-produced powder medicine packed in.Objects were divided randomly into three groups,and were given 2,5,10 grains dose separately.Then every object was scanned by CT and their attitudes were investigated on taking the medicine.Results 2 grains dose produced less gas,and stomach cavity were not filly distended;5,10 grains dose produced more gas,and stomach cavity were filly distended.After 5 minutes,CT image displayed capsule did not produce gas fully;after 10 to 15 minutes,capsule produced gas fully;after 20 minutes,gas decreased.Objects could accept 2 or 5 grains dose,but unwillingly accepted 10 grains dose.Conclusion Capsule of gas-produced powder produces gas steadily and lasts long.5 grains dose is suggested and the patient should take scan 10~15 minutes after taking capsules.

Objective The purpose of our study is to investigate the clinical value of virtual touch tissue quantification (VTQ)in the differential diagnosis of the benign and malignant liver tumor.Methods VTQ was used to inspect 53 patients with liver space-occupying lesions.Results After choosing the little lesions (the largest diameter less than 35 mm) from all the case groups,the VTQ value of the malignant group was higher than the hepatic hemangioma group (with significant differences among the different groups,P ＞0.05).Through the analysis of receiver operating characteristic curve (ROC),the optimal cutoff point as diagnosis reference value for differential diagnosis between malignant and benign groups was available.When the Area under the curve was 0.778,and with the VTQ value of 1.91 m/s,the sensitivity,specificity and Youden index was 78.6％,90.0％,0.686 respectively.The correlation analysis showed that the VTQ values of the lesion was positively correlated with the largest diameter of the lesion in the liver hemangioma group,with significant statistically differences (P ＜ 0.05).The correlation index was 0.812.Conclusions VTQ could provide a new effective way to benign and malignant liver lesions in differential diagnosis.

To gain more insights into the rice base editor (rBE3 and rBE4), we evaluated the mutation efficiency, off-target and inheritance of OsSERK1(D428N) and pi-ta(S918F) genes modified with rBE endonucleases. We predicted and analyzed the putative off-target sites of the sgRNA designed for OsSERK1(D428N) and pi-ta(S918F) by PCR amplification and Sanger sequencing. Then we further characterized the inheritance and stability of targeted base mutations and T-DNA segregation in the progeny of the self-fertilized T0 plants. Analysis of the DNA sequencing data of T0 plants of OsSERK1(D428N) revealed no nucleotide change at any of the four potential off-target sites. For OsSERK1(D428N) and Os08g07774 carry the same sgRNA targeting sites, base substitution at both two loci were detected at a frequency of 41.67%. The targeted base mutations could be transmitted readily to T1 progeny. Furthermore, genetic segregation caused the loss of T-DNA at a frequency between 25.0% and 40.9% in the T1 transgenic plants of OsSERK1(D428N) and pi-ta(S918F). These results demonstrated that the rBE3 and rBE4 systems could mediate specifically targeted base editing in one- or multi-site, and the targeted base editing could be stably inherited to next generation.

Dissolving microneedles carried drug molecules can effectively penetrate the stratum corneum of skin to improve the transdermal drug delivery. The traditional Chinese medicine acupuncture is based on the needle stimulation at a specific location (acupoint) to generate and transmit biochemical and physiological signals which alter the pathophysiological state of patients. However, the pain associated with conventional acupuncture needles and the requirement of highly trained professionals limit the development of acupuncture in non-Asian countries. The purpose of this study is to investigate whether the dissolving microneedles can be utilized as a self-administered painless replacement for acupuncture and locally released drug molecules can achieve expected therapeutic outcomes. Immunosuppressive rats were treated with acupuncture at Zusanli (ST36) acupoint using microneedles containing thymopentin. The immune functions and psychological mood of the immunosuppressed animals were examined. The proliferation of splenocytes was examined by CCK-8 assay. CD4 and CD8 expression patterns in spleen cells were detected by flow cytometry. The current study showed that use of either microneedles containing thymopentin or conventional acupuncture both resulted in immune cell proliferation, which was confirmed by flow cytometry. Furthermore, either conventional acupuncture or microneedles were able to effectively mitigate the anxiety caused by immune-suppression when applied on the ST36.