2.Experimental study on effect of arsenic trioxide on vascular smooth muscle cells
Qin LU ; Yan-Li AN ; Zi-Yu WANG ; Dong-Sheng ZHANG ; Huan-Zhang NIU ; Juan-Juan FANG ; Gao-Jun TENG ;
Chinese Journal of Radiology 2001;0(04):-
Objective To investigate the effect of arsenic trioxide(As_2 O_3)nanoparticles on rabbit vascular smooth muscle cells in vitro in comparison with normal form As_2 03.Methods The rabbit vascular smooth muscle cells were cultured in vitro.Nano and normal forms of As_2O_3 with drug concentrations of 3?mol/L were added into the cells.Cell proliferation curve was drawn according to the light absorption values of MTT test.Flow cytometry was applied to observe the apoptosis.DNA was extracted and underwent electrophoresis.Results Cell proliferation treated with the 3?mol/L concentration of As_2O_3 was inhibited. Cell growth was inhibited markedly with increased treatment time,and the inhibition effect of nano drug form seemed stronger than that of normal form.MTT light absorption values of cells treated at 24,48 and 72 h showed statistically significant difference(H=10.934,15.039,15.539,P
3.Acupuncture for premature ovarian insufficiency: a systematic review and meta-analysis
Ya-Qian YIN ; Huan-Fang XU ; Yi-Gong FANG ; Hui-Sheng YANG ; Xiao-Tong LI ; Jie SHANG ; Chen-Si ZHENG ; Ming-Zhao HAO
Journal of Acupuncture and Tuina Science 2020;18(1):24-32
Objective: To analyze the effect of acupuncture versus hormone replacement therapy (HRT) for premature ovarian insufficiency (POI). Methods: China National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full-text Database (Wanfang), Chongqing VIP Database (CQVIP), China Biology Medicine Disc (CBM), Web of Science, Cochrane Library, PubMed, and Excerpta Medica Database (EMBASE) were searched up to January 31st, 2019 to identify randomized controlled trials (RCTs) evaluating the effect of acupuncture for POI. The primary outcome was the level of basal serum follicle- stimulating hormone (FSH). Secondary outcomes included serum levels of luteinizing hormone (LH), estradiol (E2) and anti-Müllerian hormone (AMH). Two authors extracted data independently and assessed the risk of bias and the methodological quality using the Cochrane's tool. Meta-analysis was conducted by RevMan version 5.3. Results: Eight eligible RCTs with a total of 496 POI patients were included in the meta-analysis. The pooled results showed that there was a significant reduction in the basal serum FSH level (MD=-5.82, 95%CI:-9.76 to -1.87, I2=82%, P=0.004) and a remarkable elevation in the basal E2 level (SMD=0.93, 95%CI: 0.34 to 1.52, I2=88%, P=0.002) in the acupuncture group when compared with the control. Subgroup analysis showed that compared with HRT, a significant decrease in the FSH level was observed in both acupuncture alone (MD=-4.53, 95%CI:-8.96 to -0.10, I2=73%, P=0.04) and acupuncture plus HRT (MD=-9.60, 95%CI:-17.60 to -1.61, I2=50%, P=0.02), while a remarkable elevation of E2 was only found in acupuncture plus HRT (SMD=1.43, 95%CI: 1.03 to 1.82, I2=0%, P<0.00001). There was no significant difference in the LH level between acupuncture and HRT (MD=-3.16, 95%CI:-9.41 to 3.10, I2=0%, P=0.32), only one trial reported AMH, and no significant difference was found between acupuncture and HRT. Conclusion: The present study indicated that acupuncture had an advantage over HRT in reducing serum FSH level and increasing serum E2 level in women with POI. However, evidence supporting the finding is limited due to the small sample size, potential methodological flaws and significant heterogeneity. Hence, this conclusion still needs to be verified by high-quality RCTs.
4.Research on cooperation between Streptococcus mutans and Lactobacilli in dental caries lesion.
Li-huan ZHANG ; Tao-sheng SUN ; Hong-liang WU ; Fang-ming SUN
West China Journal of Stomatology 2009;27(6):657-659
OBJECTIVETo analyze the count of Streptococcus mutans (S. mutans) and Lactobacilli (LB) in different groups and the cases in dental caries. To research the synergistic effect of S. mutans and LB in the process of dental caries.
METHODS110 cases with dental caries were selected and divided according to the different degree of caries, nature and ages. To culture bacteria in the selective culture medium, then count the number of colonies. The detection rate of two kinds of bacteria in different groups, means of the bacteria count and the cariogenic cases were analyzed.
RESULTSThe means of the two bacteria count increased along with the degree of caries increased (P < 0.05), and increased in the older group (P < 0.05) and the active stage (P < 0.05). The cases of two bacteria increased with the degree of caries increased (P < 0.05), and increased in the older group (P < 0.05). But there were no significant differences in evolution period and arrested caries.
CONCLUSIONThe pathopoiesis capability of S. mutans and LB enhanced with the extent of caries increased. In the older group, their synergism role play a lead position. In evolution period and arrested caries, S. mutans and LB were difference only in quantity and their solo cariogenic potential all enhanced in active stage, but there were not correlation on pathopoiesis capability and active or stationary phase.
Bacteria ; Dental Caries ; Dental Caries Susceptibility ; Female ; Humans ; Lactobacillus ; Saliva ; Streptococcus mutans
5.Effect of autophagy inhibitor chloroquine on the proliferation of PASMCs induced by hypoxia.
Huan-Mian ZHU ; Ran CHEN ; Feng XUE ; Yang-Ping SHENTU ; Xiao-Fang FAN ; Yong-Sheng GONG ; Hong-Yu ZHANG ; Xiao-Xia KONG
Chinese Journal of Applied Physiology 2014;30(1):8-12
OBJECTIVETo investigate the role of autophagy inhibitor chloroquine (CQ) in the proliferation of pulmonary arterial smooth muscle cells (PASMCs) in hypoxia conditions.
METHODSThe following groups in this study were set up: control group, hypoxia group, 50 micromol/L CQ + hypoxia group, 50 micromol/L CQ group. The viability of PASMCs in every group was detected by MTT assay. Autophagic vacuoles in the cells were observed by MDC staining. Protein expression of microtubule associated protein light chain 3 (LC3) was measured by Western blot. Migration of PASMCs was detected by wound healing assay.
RESULTSCompared with control group, no effect on the viability of PASMCs was observed treated by CQ alone. In 1% hypoxia group, cell viability increased significantly compared with that in control group. The number of autophagic vacuoles and the rate of cell migration and also protein expression of LC3-II were also markedly increased. Compared with hypoxia group, addition of CQ increased the number of autophagic vacuoles and the levels of LC3-II protein, but decreased the proliferation and migration of PASMCs.
CONCLUSIONHypoxia could activates autophagy and contributes to proliferation and migration of PASMCs, and autophagy inhibitor CQ could decrease the effect of hypoxia on PASMCs through inhibiting autophagy process.
Autophagy ; drug effects ; Cell Hypoxia ; Cell Movement ; Cell Survival ; Cells, Cultured ; Chloroquine ; pharmacology ; Humans ; Microtubule-Associated Proteins ; metabolism ; Myocytes, Smooth Muscle ; drug effects ; Pulmonary Artery ; cytology
6.Experimental study of human skin fibroblasts cultured in three-dimension(3D).
Zhi-guo LIU ; Jing-ning HUAN ; Yu-lin CHEN ; Sheng-de GE ; Zhi-yang FANG ; Tian-xiang OUYANG ; Xin XING
Chinese Journal of Plastic Surgery 2004;20(6):443-446
OBJECTIVETo investigate the biological characters of human skin fibroblasts in fibroblast populated collagen lattice (FPCL).
METHODSThe human fibroblasts were cultured in 3D and the collagen of the rat tail was also prepared. They were examined with the comprising cell cycle and apoptosis, mRNA expression of TGF beta1, and fibronectin, and cell morphology.
RESULTSThe flow cytometry showed that the G0/G1, stage cells were 79% +/- 3%, 87% +/- 2% after the 7 days and 14 days separately, and there were not apoptosis peak observed. RT-PCR analysis revealed that the mRNA expression of TGF beta1, and fibronectin had no difference between human skin fibroblasts cultured in 3D and 2D. Electron microscope showed the cells were plenty of chromatin and organelles.
CONCLUSIONSThe proliferation of the human skin fibroblasts in FPCL is slow, but its biological viability is better.
Animals ; Cell Culture Techniques ; Cell Division ; Cells, Cultured ; Collagen ; Extracellular Matrix ; Fibroblasts ; cytology ; Humans ; Rats ; Skin ; cytology ; Tissue Engineering ; methods
7.Study of 161 chronic idiopathic myelofibrosis patients for clinicopathological staging.
Ai-ning LIU ; Hui-shu CHEN ; En-bin LIU ; Li-huan FANG ; Qing-ying YANG ; Lin-sheng QIAN
Chinese Journal of Hematology 2006;27(3):178-182
OBJECTIVETo investigate the significance of clinicopathological stage of chronic idiopathic myelofibrosis (CIMF) in WHO classification of 2001.
METHODSHistopathological analysis of bone marrow biopsy plastic-embedded sections stained with H-G-E and Gomori's stains and clinical features of 113 cases previously diagnosed as primary myelofibrosis (PMF) and 48 cases MPD-U (total of 161 cases which including male 79 and female 82) were studied retrospectively.
RESULTSThere was no significant differences on the clinical features among the cellular phase, collagen fiber phase, sclerotic phase and osteomyelosclerosis of 113 previously diagnosed patients. According to WHO classification 2001 of CIMF, previously diagnosis in 48 cases with MPD-U was WHO pre-CIMF, and in 113 cases with PMF was WHO CIMF-Fs. There were significant differences between of WHO pre-CIMF and WHO CIMF-Fs about clinicopathological features except age. The percentage of immature granulocytes, normoblasts, lymphocytes in peripheral blood, the size of hepatosplenomegaly, and the percent age of tear drop-like red blood cells in pre-CIMF were significantly lower than those in CIMF-Fs (P < 0.05). However, the number of hemoglobin and platelets in patients with pre-CIMF were significantly higher than that with CIMF-Fs (P < 0.01).
CONCLUSIONpre-CIMF and CIMF-Fs in clinical and histopathological features were different development stage of CIMF, while osteomyelosclerosis is a variant of CIMF, but not an independent disease.
Adult ; Aged ; Biopsy ; Bone Marrow ; pathology ; Chronic Disease ; Female ; Humans ; Male ; Middle Aged ; Primary Myelofibrosis ; classification ; pathology ; Thrombopoiesis
8.Plasma visfatin level in obese patients of nondiabetic first-degree relatives of type 2 diabetes
Jun LIU ; Ying ZHA ; Fang WANG ; Zao-Ping CHEN ; Jiong XU ; Li SHENG ; Huo-Geng XU ; Xiao-Huan LIAO ; Yu-Ling GUO ;
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
The plasma visfatin,endothelium-dependent artery dilation and intima-media thickness of common carotid arteries were measured in first-degree relatives of type 2 diabetes,obese patients and control subjects.Regional body fat were detected by MRI.The result suggested that plasma visfatin levels were significantly higher in obese subjects than those in non-obese subjects,and hypervisfatinemia is independently associated with fasting blood glucose.
9.Analysis of the epidemiological characteristics and response to public health emergencies in Putuo District of Shanghai from 2004 to 2019
Fang HU ; Zhen PENG ; Huan-sheng ZHANG
Shanghai Journal of Preventive Medicine 2021;33(12):1187-1190
ObjectiveTo analyze the epidemiological characteristics of public health emergencies in Putuo District and provide scientific evidence to effectively respond to public health emergencies in the future. MethodsDescriptive epidemiological methods were used to analyze the data of public health emergencies reported by the national online system in Putuo District from 2004 to 2019. ResultsA total of 59 public health emergencies were reported from 2004 to 2019, during which 1 252 patients and 6 death cases were involved. From 2004 to 2019, the number of public health emergencies in Putuo District first increased and then decreased, fluctuating at a low level yearly. Most of the emergencies were general events (72.88%, 43/59). 69.49 percent (41/59) of the events were infectious diseases, and 67.80 percent (40/59) occurred in schools and child care institutes, with primary schools with the highest number of events. The peaks of infectious disease events occurred in June and November, while the majority of occupational poisoning and heat stoke events occurred in July and August, with 4 patients dead from heat stroke. The medians of events detection, verification, reporting and controlling timelines were 10.81, 3.00, 1.70 and 307.40 h; different categories of events varied in their detection and controlling time interval (all
10.Morphologic and clinical study of 131 cases of plasma cell myeloma.
Hui-shu CHEN ; En-bin LIU ; Ting-ting WANG ; Ren-chi YANG ; Li-huan FANG ; Qing-ying YANG ; Ji-yong GAO ; Ming-hua YU ; Lin-sheng QIAN
Chinese Journal of Pathology 2004;33(1):44-48
OBJECTIVETo study the characteristics histologic and cytologic features and clinical usefulness of plasma cell myeloma (PCM) subtyping according to WHO PCM classification.
METHODSBone marrow biopsy plastic-embedded sections were stained with H-G-E and Gomori's stains, and bone marrow aspirate smears were stained with Wright's stain. The clinicopathologic findings were then analyzed.
RESULTSOf the 131 cases with PCM, three types of growth patterns were noted: interstitial (21 cases, 16.0%), nodular (46 cases, 35.1%) and packed (64 cases, 48.9%). Besides, there were three cytologic subtypes: mature plasma cell type (43 cases, 32.8%), immature (81 cases, 61.8%) and pleomorphic (7 cases, 5.3%) types. The age of patients with mature plasma cell type was significantly higher than that of immature type (P = 0.005); and the number of tumour cells in bone marrow smears was significantly higher than that of immature type (P = 0.003). The numbers of WBC and platelets in peripheral blood were also significantly higher than that of pleomorphic type (P = 0.024, P = 0.002, respectively). On the other hand, the number of platelets in peripheral blood of immature type was significantly higher than that of pleomorphic type (P = 0.019). Marrow fibrosis was more frequently observed in immature type than in mature plasma cell type (P = 0.000). The incidence of marrow fibrosis and osteolytic lesions was higher in high risk group than in low risk group (P = 0.000, P = 0.023 respectively). Twenty-one cases (56.8%) of the 37 cases treated with MP or MP and M2 chemotherapeutic regimens showed good response. However, there was no significant difference in treatment response and survival between different subtypes.
CONCLUSIONSEach subtype of PCM carries different clinicopathologic features in some aspects. The classification carries important value in pathologic diagnosis and probably in predicting prognosis.
Adult ; Aged ; Aged, 80 and over ; Biopsy ; Bone Marrow Examination ; Female ; Humans ; Immunophenotyping ; Male ; Middle Aged ; Multiple Myeloma ; classification ; immunology ; pathology ; Prognosis