1.Changes of sympathovagal balance in endotoxemia rats
Jian HUANG ; Zhi-Huan YANG ; Nian-Chun ZHANG ; Li-Yong CHEN ; Jian ZHOU ;
Chinese Journal of Trauma 2003;0(10):-
Objective To investigate the changes of sympathovagal balance and the effects of va- gus stimulation on sympathovagal balance in endotoxemia rats.Methods Twenty-four Spragne Dawley rats were randomly divided into four groups.The frequency domain of heart rate variability(HRV)com- ponent was analyzed at 0 min,2 ,4 and 6 hours after intravenous injection of lipopolysaccharide(LPS, 5mg/kg)or physiologic saline,and cervical vagal nerve was stimulated(5mv,2ms,1Hz,5 min lasted, 20 min interval)when LPS or physiologic saline was injected.The levels of Noepinephine(NE)and Ace- tylcholin(ACh)were measured in liver tissues.Results Normalized low frequency(LFnm),hormali- zed high frequency(HFnm),very low frequency(VLF),LF/HF values and liver ACh were significantly increased(P<0.05)and the level of liver NE was significantly decreased (P<0.05)after LPS admin- istration.Vagal nerve stimulation markedly increased HFnm but decreased LFnm,VLF,LF/HF values, and the liver ACh also significant increased(P<0.05 ).Conclusion The results suggest that the ac- tivity of sympathetic and vagal nerve was increased during endotoxemia,but the sympathetic activity was more excitable than that of vagal nerve.Vagal nerve stimulation increased the tone of vagus nerve while the tone of sympathetic nerve was decreased in this study.This may be beneficial for anti-inflammatory activity of vagal nerve.
2.Goblet cell carcinoid of appendix: report of two cases.
Xue-dong ZHANG ; Chun-nian HE ; Jin-ping ZHAI ; Huan-fen ZHAO ; Chen CHEN ; Wei-dong SHI
Chinese Journal of Pathology 2006;35(2):126-127
Adenocarcinoma, Mucinous
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pathology
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Aged
;
Appendectomy
;
methods
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Appendiceal Neoplasms
;
pathology
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surgery
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Appendicitis
;
pathology
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Appendix
;
pathology
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Carcinoid Tumor
;
pathology
;
surgery
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Humans
;
Male
3.Primary Hodgkin lymphoma of the intestinal tract: report of a case.
Wei-Dong SHI ; Chun-Nian HE ; Huan-Fen ZHAO ; Ping LI ; Chen CHEN ; Xiu-Zhi ZHANG ; Shu-Yan ZHANG
Chinese Journal of Pathology 2008;37(7):493-494
Aged
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Female
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Hodgkin Disease
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diagnosis
;
pathology
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Humans
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Reed-Sternberg Cells
;
pathology
4.Relationship between HPVtype16/18 status and the development of cervical intraepithelial neoplasia.
Ming-tang XU ; Chun-nian HE ; Chang-tian XU ; Huan-fen ZHAO ; Shu-song WANG ; Xiu-zhi ZHANG ; Chen CHEN ; Zhi-bin HAO
Chinese Journal of Pathology 2013;42(6):400-401
Adult
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Carcinoma
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virology
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Cervical Intraepithelial Neoplasia
;
virology
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Female
;
Human papillomavirus 16
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isolation & purification
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Human papillomavirus 18
;
isolation & purification
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Humans
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In Situ Hybridization
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Middle Aged
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Papillomavirus Infections
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Uterine Cervical Neoplasms
;
virology
5.Detection of TERC gene amplification by fluorescence in-situ hybridization in cervical intraepithelial lesions.
Yan-long YUAN ; Chun-nian HE ; Ming-tang XU ; Cui-qing XU ; Yu-ning SUN ; Huan-fen ZHAO ; Chen CHEN
Chinese Journal of Pathology 2011;40(3):182-186
OBJECTIVETo explore the feasibility and practical value of fluorescence in situ hybridization (FISH) detection of TERC gene amplification in cervical intraepithelial lesions (CIN) and squamous cell carcinoma (SCC).
METHODSTissue microarray was constructed to cover 150 cases of various cervical conditions, including 24 cases of normal cervical mucosa, 78 cases of CINs (CINI, 25 cases; CINII, 21 cases and CINIII, 32 cases) and 48 cases of SCC. FISH was used to detect TERC gene amplification.
RESULTSTERC gene amplification was detected in 8% (2/25) CINI, 47.6% (10/21) CINII, 71.9% (23/32) CINIII and 87.5% (42/48) SCC. There were significant differences among these groups (P < 0.05). The amplification rates of TERC gene in SCC, CINIII and CINII were significantly higher than those of normal cervical epithelium and CINI (P < 0.05). Significant differences were also observed among CINI and CINII, CINIII and SCC (P < 0.05), and between CINII and SCC (P < 0.05). There were no significant differences between normal cervical epithelium and CINI, CINII and CIN III, and between CINIII and SCC (P > 0.05). FISH detection of amplification of TERC gene in CINI and CINII-III demonstrated the following statistics: sensitivity of 62.3%, specificity of 92.0%, accuracy of 71.8%, positive and negative predictive values of 94.3% and 53.5%, respectively.
CONCLUSIONSFISH detection is a reliable method in detecting TERC gene amplification using paraffin tissue sections. When histological evaluation becomes difficult, TERC amplification detectable by FISH may offer a diagnostic distinction of CINI from CINII. Moreover, TERC amplification may be used as a biomarker in predicting CIN progression to invasive cancer.
Adenoma ; diagnosis ; genetics ; Adult ; Aged ; Biomarkers, Tumor ; analysis ; Carcinoma, Squamous Cell ; diagnosis ; genetics ; Cervical Intraepithelial Neoplasia ; diagnosis ; genetics ; Disease Progression ; Female ; Gene Amplification ; Humans ; In Situ Hybridization, Fluorescence ; Middle Aged ; RNA ; genetics ; Sensitivity and Specificity ; Telomerase ; genetics ; Uterine Cervical Neoplasms ; diagnosis ; genetics ; Young Adult
6.Infectious bovine rhinotracheitis viral gG expression and gG-ELISA development.
Bang-Fen YAN ; Zeng CHEN ; Shu-Huan ZHANG ; Xiang-Mei LIN ; Ying-Yu CHEN ; Yan-Jie CHAO ; De-Xue LI ; Nian-Hua SONG ; Huan-Chun CHEN ; Ai-Zhen GUO
Chinese Journal of Biotechnology 2007;23(5):806-811
Taking the genome DNA of Infectious Bovine Rhinotracheitis Virus (IBRV) as the template, the gG gene was amplified with PCR and cloned into the T cloning vector pMD18-T. After being identified by restriction digestion and DNA sequencing, the insert was subcloned into the expression vector pGEX-KG. Sodium docecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot assay showed that this gene was expressed as both soluble form and inclusion body by the transformed E. coli BL21 strain (DE3). The fusion protein was purified and used as the coating antigen to develop the indirect Enzyme-Linked Immunosorbent Assay (ELISA). Comparison between this gG-ELISA and commercial IBRV gB-ELISA Kit (IDEXX) was made in the detection of 380 cow serum samples. The results demonstrated an agreement of 92%. By using this novel gG-ELISA, 1248 cow serum samples were tested and the average positive rate of IBRV antibodies for imported cows is 21.7%, while the positive rate ranged greatly from 0.0%-41.5% for Hubei local Chinese Black and White Dairy Cows.
Animals
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Antibodies, Viral
;
blood
;
immunology
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Antigens, Viral
;
genetics
;
immunology
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Cattle
;
Cloning, Molecular
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Enzyme-Linked Immunosorbent Assay
;
methods
;
Escherichia coli
;
genetics
;
metabolism
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Female
;
Herpesvirus 1, Bovine
;
genetics
;
immunology
;
Male
;
Recombinant Proteins
;
biosynthesis
;
genetics
;
immunology
;
Sensitivity and Specificity
;
Viral Proteins
;
biosynthesis
;
genetics
;
immunology
7.The effect of celecoxib on tissue factor expression in pancreatic cancer cells.
Hui-yuan WANG ; Yin-mo YANG ; Yan ZHUANG ; Huan-nian CHEN ; Yuan-lian WAN ; Yan-ting HUANG
Chinese Medical Journal 2007;120(20):1753-1756
BACKGROUNDTissue factor (TF) is overexpressed in many malignant tumours and is linked to the pathogenesis and prognosis of such malignancies. In vitro studies have proved that reduced expression of TF has inhibitory effect on the angiogenesis and cell proliferation of the malignant tumour. Therefore, TF suppression has been raised as a possible treatment for malignant tumours. Here we investigated the effect of celecoxib on TF expression induced by tumour necrosis factor alpha (TNFalpha) in PANC-1 cells and a possible molecular mechanism underlying the celecoxib effect.
METHODSVarious doses of celecoxib solution were added to standard cell numbers of PANC-1 cells mixed with equal dose of TNFalpha for 6 hours. The expression of tissue factor was detected quantitatively by Western blot, whilst the activation of nuclear factor kappaB was tested by electromobility shift assay.
RESULTSAs the doses of celecoxib increased, the tissue factor expression was decreased in PANC-1 cells and so was the activation of nuclear factor kappaB.
CONCLUSIONSCelecoxib can downregulate the expression of tissue factor induced by TNFalpha in PANC-1 cells. This antitumour effect of celecoxib can be explained indirectly via its suppressive role in activation of nuclear factor kappaB.
Celecoxib ; Cell Line, Tumor ; Cyclooxygenase 2 Inhibitors ; pharmacology ; Gene Expression Regulation ; drug effects ; Humans ; NF-kappa B ; metabolism ; Pancreatic Neoplasms ; metabolism ; pathology ; Pyrazoles ; pharmacology ; Sulfonamides ; pharmacology ; Thromboplastin ; genetics ; Tumor Necrosis Factor-alpha ; antagonists & inhibitors
8.In vivo Breath Analysis by Extractive Electrospray Ionization-Mass Spectrometry for Investigation of Metabolic Responses to Traditional Chinese Medicine Massages
Teng-Gao ZHU ; Jing HAN ; Jun-Wen SHU ; Mu-Fang KE ; Dan WANG ; Wen-Jie LIU ; Nian-Xiang LIN ; Huan-Wen CHEN
Chinese Journal of Analytical Chemistry 2018;46(3):400-405
Traditional Chinese Medicine massage is a kind of physiotherapy which affects on specific parts of the body surface by means of training to regulate the function of the body to achieve the therapeutic effect. In this work,under positive detection model, the chemical fingerprint of exhaled breath from volunteers before and after receiving Traditional Chinese Medicine massage within m/z 50-1000 were detected by extractive electrospray ionization-mass spectrometry (EESI-MS). And through high resolution mass spectrometry analysis, the metabolites such as epinephrine (m/z 184. 0889), 3-(3-hydroxyphenyl) propionic acid (m/z 167.0615) and L-tryptophan (m/z 205. 0933) were successfully identified. Besides, chemical fingerprints of volunteers before and after receiving Traditional Chinese Medicine massage under different health condition were clearly differentiated via partial least squares discrimination analysis (PLS-DA). The results showed that Traditional Chinese Medicine massage could significantly change the metabolic process of volunteers. Moreover, it further indicated that the established method could provide a real time fashion to follow metabolic changes caused by Traditional Chinese Medicine massage.
9.Expression of Twist in papillary thyroid carcinomas and its roles in differential diagnosis.
Chun-Nian HE ; Lili HE ; Jin Q CHENG ; Shi-Chao CHEN ; Huan-Fen ZHAO ; Jin-Ping ZHAI ; Jing ZHANG
Chinese Journal of Pathology 2008;37(1):35-39
OBJECTIVETo study Twist expression in thyroid papillary carcinoma (PTC) by immunohistochemistry and to assess its usefulness as marker in the differential diagnosis of PTC, follicular adenomas (FA) and benign papillary lesions (BPL).
METHODSFifty cases of PTC, 48 cases of FA and 47 cases of BPL were evaluated using manual tissue chip and SP immunohistochemical stain to detect the expression of Twist and HBME-1, and comparing the staining to that of cytokeratin 19 (CK19).
RESULTSIn PTC, positive rates of Twist, HBME-1 and CK19 were 100% (48/48), 94.0% (47/50) and 78.0% (39/ 50) respectively; in FA, positive rates were 0, 6.7% (3/45) and 0 respectively; in BPL, positive rates were 7.0% (3/34), 2.1% (1/47) and 0, respectively. The differences between PTC and FA and between PTC and BPL were both statistically significant (P = 0. 000). The sensitivity of Twist, HBME-1 and CK19 was 100%, 94.0% and 78.0%; the specifity was 96.4%, 95.7% and 100%; overall accurary was 97.7%, 95.1% and 91.9%, respectively.
CONCLUSIONSPositive rates of Twist is higher than the other markers in PTC. Immunohistochemical staining of Twist has important significance in the differential diagnosis of thyroid lesions. Twist immunohistochemistry maybe helpful in diagnosis and differential diagnosis of PTC.
Adenocarcinoma, Follicular ; metabolism ; Adenocarcinoma, Papillary ; pathology ; Adenoma ; diagnosis ; metabolism ; Biomarkers, Tumor ; immunology ; Carcinoma, Papillary ; diagnosis ; metabolism ; pathology ; Carcinoma, Papillary, Follicular ; metabolism ; Diagnosis, Differential ; Galectin 3 ; genetics ; metabolism ; Immunohistochemistry ; Keratin-19 ; genetics ; Keratins ; genetics ; metabolism ; Nuclear Proteins ; genetics ; metabolism ; Thyroid Neoplasms ; diagnosis ; metabolism ; pathology ; Thyroid Nodule ; pathology ; Twist-Related Protein 1 ; genetics ; metabolism
10.Diagnostic value of contrast-enhanced ultrasound in breast precancerous lesions
Ting YUE ; Qin CHEN ; Jun LUO ; Hao WU ; Wanyue DENG ; Lina TANG ; Yijie CHEN ; Zhongshi DU ; Lichun YANG ; Xiaomao LUO ; Yinghua NIAN ; Zhihong LYU ; Ehui HAN ; Huan LI ; Yinrong CHENG ; Lei YANG ; Lijun YUAN ; Yong YANG ; Yilin YANG ; Yan CHENG ; Zizhen ZHANG ; Baoyan BAI ; Shengli WANG ; Honghong XUE
Chinese Journal of Ultrasonography 2017;26(12):1048-1052
Objective To evaluate the diagnostic value of contrast-enhanced ultrasound in breast precancerous lesions . Methods Retrospectively analyzed the contrast-enhanced ultrasound model and angiographic predictive model of 465 cases of the A prospective multicenter study of breast nodules contrast-enhanced ultrasound" that led the Sichuan Provincial People′s Hospital from January 2016 to April 2017 ,which included 69 cases of breast precancerous lesions and 396 other types benign lesions ,and the sensitivity ,specificity and accuracy of the diagnosis of breast precancerous lesions were calculated . Results The sensitivity of ultrasound predictive model for the diagnosis of precancerous lesions was 60 .9% and AUC was 0 .681 . Precancerous lesions mainly showed non-concentricity , increased homogeneity , and increased lesions;other types of benign lesions mainly showed non-centripetal ,high uniformity enhancement and lesion size unchanged . Conclusions Contrast-enhanced ultrasound shows a potential value in the differential diagnosis of precancerous lesions and other types of benign lesions ,that can help clinicians to take early intervention measures for breast precancerous lesions ,but there are still many problems to be solved .