Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Ventricular Premature Complexes ; therapy

Objective To study the expression and clinical significance of COX-2 and the relation- ship between COX-2 and PTEN in endometrial adenocarcinoma(EAC).Methods The expression of COX-2, PTEN protein was detected by SP-immunohistochemical method in EAC,endometrial intraepithelial neoplasia, corresponding normal endometrium.Results The positive rates of COX-2 protein increased from normal en- dometrium(13.33%) to endometrial intraepithelial neoplasia(50.00%) and adenocarcinoma(64.58 %)(P

Objective To study the clinical significance of detecting the levels of homocysteine,lipoprotein(a)and high sensi-tivity C-reaction protein in serum from patients with diabetes mellitus.Methods Serum Hcy,Lp(a)and hsCRP levels were detected from 30 patients of diabetes mellitus with microangiopathy,and 30 patients of simple diabetic,45 heslthy individuals as normal controls,all the data were analysed by SPSS13.0 software.Results The levels of Hcy,Lp(a)and hsCRP in dia-betes mellitus with microangiopathy group were higher than that of normal controls and simple diabetic group.In all groups, the positive rate of Lp(a)was highest (P<0.05).Detecting the serun Hcy combined with Lp(a)and hsCRP had higher positive rate then single detecting (P<0.05).Conclusion The generation and development of diabetes mellitus were corre-lated with the serum levels of Hcy,Lp(a)and hsCRP.Detecting the serum Hcy combined with Lp(a)and hsCRP have more clinical significance than single detecting.Serum Hcy,Lp(a)and hsCRP level has the remarkable significance to diagnosis and prediction diabetes mellitus with microangiopathy.

OBJECTIVETo study the TCM syndrome differentiation rules in coronary artery bypass grafting (CABG) patients during peri-operation period.

METHODSAccording to the Guideline for Clinical Trail of Traditional Chinese New Drugs and experience of Professor DENG Tie-tao, the standard of TCM syndrome differentiation for CABG patients was established, with which 161 CABG patients were classified before and two weeks after CABG operation.

RESULTSBefore CABG, the qi stagnation and blood stasis syndrome type was the type most commonly seen (46.0%), the next in turn was the phlegm stagnation type (24.8%), the yang-qi deficiency type (15.5%), and the yin-blood deficiency type (13.7%). While after CABG, the phlegm stagnation syndrome type was the most frequently occurred one (35.9%), then in turn was the yang-qi deficiency type (30.8%), the yin-blood deficiency type (21.2%) and the qi stagnation with blood stasis type (12.2%).

CONCLUSIONFor patients before CABG, the pathological characteristic is mostly the deficiency in root with excess in superficiality, and the commonest syndrome is the qi stagnation with blood stasis type. For patients after CABG, both deficiency and excess is the pathological characteristic, the Xin-qi and Xin-yang deficiency syndrome the commonest type, and with phlegm as the main pathologic factor.

Adult ; Aged ; Aged, 80 and over ; Coronary Artery Bypass ; Coronary Artery Disease ; drug therapy ; surgery ; Diagnosis, Differential ; Female ; Humans ; Intraoperative Period ; Male ; Medicine, Chinese Traditional ; Middle Aged

Humans ; Siblings ; Forensic Medicine ; Forensic Genetics

OBJECTIVE Based on the methods of microdialysis,HPLC-MS/MS and gene chip tech-nology,the mechanism of Baicalin and Geniposide(BC/GP)against excitatory amino acid toxicity in ce-rebral ischemia was studied. This will provide guidance for the clinical application of BC/GP and the study of excitatory amino acid toxicity in cerebral ischemia.METHODS (1)Microdialysis technique and HPLC-MS/MS was performed to study the pharmacodynamics of BC/GP against cerebral ischemia. ①18 SD rats with body weight of(280±20)g were randomly divided into control group,treatment groups with BC/CP at low dose,medium dose and high dose(equal to the dosage of crude drugs for 30 mg·kg-1, 45 mg·kg-1and 60 mg·kg-1respectively).Rats in each group were given intragastric administration for seven days to establish cerebral ischemia model. Then, microdialysis probe was applied to collect cerebrospinal fluid from hippocampus before and after cerebral ischemia. ② First, we established the HPLC-MS/MS method for measuring drugs and excitatory amino acids.Then we detected the microdi-alysis samples and observed their changes in animals.(2)The mechanism of BC/GP against excitatory toxicity of cerebral ischemia were observed at gene level by chip technique. ① 16 SD rats with body weight of 240±20 g were randomly divided into sham group, model group, treatment group of BC(60 mg·kg-1),treatment group of GP(60 mg·kg-1)and treatment group of BC/GP(7:3)(60 mg·kg-1).Rats in eachgroup were given intragastric administration for seven days to establish cerebral ischemia model. Then the rats were sacrificed,and the hippocampus were rapidly harvested and stored at-80℃for further detection. ②After the quality inspection of the hippocampal,the qualified samples were subjected to detect the levels of neurotransmitter receptor gene in the ischemic of rats by gene chip technology.Finally,the results were analyzed by the method of Δ ΔCt.RESULTS (1)Only three compounds includ-ed GP,glutamic acid and aspartic acid were detected in microdialysis samples by HPLC-MS/MS.The concentration of GP increased and lasted for 120 min with a significant dose-dependent after cerebral ischemia.Compared with low dose group,the AUC(0-t),MRT(0-∞),Cmaxand t1/2zin high-dose group showed significant difference(P<0.01).Compared with the model group,the levels of glutamic acid and aspartic acid in the treatment groups decreased significantly,especially in the middle and high dose groups.(2) 89 genes in the neurotransmitter receptor gene signaling pathway were detected by gene chip technol-ogy. There were 22 genes with |Fold Regulation|>1.5 in the model group, compared with the sham group.Five of the 22 genes showed statistically significant differences,including Grin2c(2.9026),Chrna7 (-1.5877), and Tacr2 (-1.7695). Htr3a (-1.8172) and Grm6 (-2.3527). There were 5 genes with |Fold Regulation|>1.5 in the BC group, compared with the model group, Two of them exhibited statistically significant differences,including Brs3(1.797)and Grin2c(-1.7979).There were 14 genes with|Fold Reg-ulation|>1.5 in the GP group, compared with the model group. Three of them displayed statistically significant differences,including Hcrtr2 (-1.6584), Sctr (-3.8524) and Grin2c (-4.8408). Compared with model group, the genes of |Fold Regulation|>1.5 in BC/GP (7:3) group are 5, and only one of them showed a significant differences. CONCLUSION (1)After administration of BC and GP,GP can cross the blood-brain barrier and reduce the release of excitatory amino acids in the hippocampus. (2) BC/GP can inhibit the interaction between excitatory amino acids and excitatory amino acid receptors and attenuate the toxicity of excitatory amino acids by down-regulating the expression of glutamic acid receptor Grin2c gene.(3)BC/GP may exert their brain protection effect by reducing the release of excit-atory amino acids and inhibiting the expression of excitatory amino acid receptors.

BACKGROUND: Gelatin methacryloyl has been widely used in the field of tissue engineering, as it has suitable biological properties, tunable physical and chemical properties, non-cytotoxic and non-immunogenicity.OBJECTIVE: To summarize the latest advances in the repair of skin and soft tissue damage using gelatin methacryl hydrogel materials. METHODS: PubMed and SciFinder were retrieved for articles concerning gelatin methacrylate hydrogels published from January 2007 to August 2017. The key words were "seed cell, skin regeneration, wound vascularization, gelatin, gelatin methacryloyl, scaffold material, wound healing, microenvironment, tissue construction, skin tissue engineering". RESULTS AND CONCLUSION: The gelatin methacryloyl hydrogel is very similar to the native extracellular matrix, and has a cell adhesion site, a matrix metalloproteinase-reactive peptide-based sequence and a cross-linkable property exhibiting good tissue affinity. The hydrogel has adjustable physical and chemical properties, a certain degree of adhesion and biodegradability, which make it an ideal cell scaffold, allowing all kinds of cells to proliferate and extend on its surface. Therefore, gelatin methacrylamide hydrogel has broad prospects in the skin tissue engineering, which can accelerate wound vascularization and epithelial tissue regeneration, improve wound healing rate, reduce the probability of infection, and improve the patient's quality life. The gelatin methacrylamide hydrogen is proved to provide an efficient and portable gel dressing for burn wounds and war wounds, and it can also be used to fill skin and soft tissue defects such as trauma and ulcers, and cover cosmetic incisions.

BACKGROUND: Compound gelatin-methacryloyl (GelMA) hydrogel has been shown to have unique advantages in bone, cartilage, myocardium, and vascular regeneration.OBJECTIVE: To summarize the novel progress of compound GelMA hydrogel for bone tissue engineering. METHODS: PubMed database and CNKI database from 2010 to 2017 were searched by using the keywords of "gelatin, methacrylamide, hydrogel, hydroxyapatite, bone tissue engineering, bone regeneration, seed cells, microenvironment" in Chinese and English, respectively. RESULTS AND CONCLUSION: Inorganic components can be added in a specific way into the compound GelMA hydrogel to prepare tissue-engineered bone using photolithography, microfluidics, microfabrication and 3D printing techniques. The prepared tissue-engineered bone has similar structural, mechanical and biological properties to natural bone tissue, and importantly, it has osteogenic ability. Gene-modified seed cells that are co-cultured with the compound GelMA hydrogel in a 3D environment are found to grow well and express some genes related to bone regeneration and vascular regeneration. Therefore, the compound GelMA hydrogel has a good osteogenesis effect in vitro,which is an excellent material for bone tissue engineering.

To investigate the application of diagnosis methods for coronary artery disease (CAD) and the status quo of its syndrome typing. The literature content analysis was used in this study. The frequency statistics was performed by comprehensively collecting CAD (thoracic obstruction) syndrome typing correlated literatures, designing content analysis tables, extracting information such as typing methods, typing elements, and syndrome typing, and so on. Results showed that blood stasis, yin deficiency, qi deficiency, yang deficiency, phlegm turbidity, and other syndrome elements extensively exist in literatures concerning CAD syndrome typing. Modem doctors prefer to use syndrome typing of qi, blood, jinye, and eight principles in syndrome typing of CAD more frequently. The asthenia in origin and asthenia in superficiality has been widely recognized as the basic syndrome of CAD.
Coronary Artery Disease ; classification ; diagnosis ; Diagnosis, Differential ; Humans ; Medicine, Chinese Traditional ; methods ; Yang Deficiency ; diagnosis ; Yin Deficiency ; diagnosis

OBJECTIVETo identify the expression characteristics of the 1700001022RIK (RIKEN cDNA 1700001022) gene in mice and explore its function by bioinformatic analysis.

METHODSUsing the expression profile of gene microarray, we detected the expression of a new testis-specific gene, 1700001022RIK, in mice. We analyzed its expression characteristics in the testis tissue and their changes in different developmental stages of the testis by RT-PCR, real-time RT-PCR, Western blot, and immunohistochemistry. We performed bioinformatic analysis using a bioinformatic software.

RESULTSThe 1700001022RIK gene was specifically expressed in the mouse testis in an age-dependent manner, most highly in the adult mice. The 1700001022RIK protein was mainly expressed in the spermatogonia, spermatocytes, and round spermatids of the adult mice. Bioinformatic analysis showed that the 1700001022RIK protein amino acid sequence had a high similarity in human and mice, which indicated that this gene was highly conserved in mammals.

CONCLUSION1700001022RIK is a testis-specific gene mainly expressed in the spermatogonia, spermatocytes, and round spermatids of seminiferous tubules, which might be involved in the regulation of spermatogenesis.

Age Factors ; Animals ; Blotting, Western ; Computational Biology ; DNA, Complementary ; Gene Expression ; Genomics ; Male ; Mice ; Molecular Chaperones ; genetics ; Seminiferous Tubules ; Spermatids ; Spermatocytes ; Spermatogenesis ; genetics ; Spermatogonia ; Testis