Transcatheter arterial chemoembolization (TACE) is the main treatment method for advanced hepatocellular carcinoma (HCC). As a non-invasive functional MR imaging, diffusion weighted imaging (DWI) can relfect the functional changes in tumor before morphologic changes. In the follow-up of HCC after TACE, DWI can detect new lesions and distinguish necrotic neoplastic tissue and residual neoplastic tissue timely and accurately.

Objective To investigate the effect of thalidomide and As_2O_3 to the secretion of VEGF of the cell of ANLL and study if there is coorperation in thalidomide and As_2O_3.Methods From October 2001 to February 2003,23 patients with ANLL were analyzed.Heparinized marrow was taken,then it was putt into Ficoll-isopaque solution to be purified for MNC,which was cultured in the RPMI 1 640 medium,placed in 5% CO_2 saturation and 37℃ to proliferation.Choosing the cells in logarithm growth phase,adding cultural solution to abstaining the cellular density of 2?10~9/L and then putting it into 24-well plates.There are 4 groups :contrast group,thalidomide(300 ?g?mL~ -1 )group,As_2O_3(25 ?mol/L)group,and thalidomide(300 ?g?mL~ -1 )andAs_2O_3(25 ?mol/L)group,cultured for 72 hours.Examine the concentration of VEGF in upper liquid with ELISA method.Results The concentration of VEGF in upper liquid of 4 groups were respectively(1 582.67?281.51)ng/L、(1 206.29?264.87)ng/L、(941.72?236.34)ng/L、(639.12?211.98)ng/L.There were statisticd difference(P

Acclimatization ; physiology ; Animals ; Animals, Newborn ; Chickens ; Cold Temperature ; Endocrine Glands ; physiology ; Female ; Male ; Rats ; Rats, Wistar

Objective to observe the effect of lung and spleen mixture on lung spleen deficiency AECOPD with Ⅱ respiratory failure of TCM symptoms integral,nutrition,blood gas and related indicators.Methods in accordance with AECOPD with Ⅱ type of respiratory failure need to use the noninvasive or have a respirator assisted ventilation,and conform to the type of TCM for patients with spleen-deficiency type,40 cases were divided into two groups,control group to the conventional western medicine therapy and Chinese medicine group on the basis of western medicine treatment for reasons of Yifeijianpi mixture,oral or tube feeding,a week for a period of treatment,observation of patients before and after treatment of TCM symptoms and physical signs integral,APACHE Ⅱ score,serum albumin,pre-albumin,neutrophil percentage,CRP and blood gas analysis and related indicators was conducted.Results The results show that the TCM group can improve the patients' TCM symptoms and physical signs integral,there was significant difference between the two groups after treatment (P＜ 0.05);compare the serum albumin,pre-albumin,transferrin,neutrophil percentage,CRP and arterial blood gas between two groups of patients after treatment,Chinese medicine group is superior to the control group,with statistical significance (P＜ 0.05);And compared with control group,the APACHE Ⅱ improvement situation of Chinese medicine group were superior to control group after the treatment after a week,with statistical significance (P＜ 0.05).Conclusion on the basis of using conventional western medicine treatment,treatment based on the theory of ridging raw gold formulae of Yifeijianpi mixture has good effect in the treatment of AECOPD combined type Ⅱ respiratory failure,and can improve the nutritional status of patients and the indicators of arterial blood gas analysis.

The content of SO2 in Paeoniae Radix Alba (RPA) was determined by the method documented in Chinese Pharmacopoeia (CP) 2010 edition to validate the repeatability of the method for evaluating RPA, and the contents of paeoniflorin sulfonate in both the residual material and distilled solution of RPA were determined by HPLC to study the transformation of paeoniflorin sulfonate to SO2 by HCl. It was found that the repeatability of the method in CP for evaluating RPA is unacceptable, and paeoniflorin sulfonate was detectable in both the residual material and distilled solution of RPA even at "the end point" of SO2 determination, merely about 50% of paeoniflorin sulfonate was transformed to SO2 by HCl, indicating that the current SO2 determination method in CP is not able to accurately quantify SO2 in RPA. It is recommended that more special method for determining SO2 content in RPA should be developed regarding the chemical characteristics of sulfur-fumigated RPA.
Chemistry Techniques, Analytical ; methods ; Chemistry, Pharmaceutical ; Drugs, Chinese Herbal ; analysis ; Fumigation ; Glucosides ; analysis ; Monoterpenes ; analysis ; Paeonia ; chemistry ; Pharmacopoeias as Topic ; standards ; Sulfur Dioxide ; chemistry

To investigate the curative effect of minimally invasive sclera buckling on single retinal detachment.METHODS:Totally, 100 cases of patients with retinal detachment ( 106 eyes ) enrolled in our hospital were randomly divided into observation group and control group, 53 eyes in each group. Patients in observation group were treated with minimally invasive sclera buckling, while patients in control group received traditional limbal conjunctival incision. After surgery, patients were all followed up for 6 ~18mo, during which the retinal recurrence situation, degree of vision enhancement and compliance occurrence rate was recorded. RESULTS: The retinal reattachment rate once of observation group (96. 22%) was significantly higher than that of control group (88. 68%), there was statistically significance (P<0. 05). The vision enhancement rate of observation group (84. 90%) was significantly higher than that of control group (71. 70%), there was statistically significance (P<0. 05). The compliance occurrence rate of observation group (11. 32%) was significantly lower than that of control group (32. 08%), there was statistically significance (P<0. 05).CONCLUSlON: The improved minimally invasive sclera buckling can significantly enhance the curative effect for retinal detachment, decrease the compliance occurrence rate, improve vision function, and is a scientific, practical and rigorous tool for retinal detachment treatment.

Objective To determine the inhibitive effect of interferon-a on hepatic metastasis of colon cancer. Methods Metastatic model of human colon cancer was established by orthotopic implantation of histologically intact human tumor tissue into the colon wall of nude mice.The mice were randomly divided into 4 groups:(1)Control group(receiving saline solution only);(2)5-FU treatment group;(3) IFN-?-2b treatment group;and(4)5-FU combined with IFN-?-2b treatment group(combined group). 5-FU and IFN-?-2b were given via peritoneum injection 1 time/2 days for eight weeks.The mice were killed at nine weeks.The tumors were weighed,the microvessel density(MVD) was detected,and the liver was examined histologically in order to discover the micrometastasis. Results In control group, 5-FU group,IFN-2b group and combined group,the tumor weight was(1.53?0.78)g,(0.87?0.59)g,(0.81?0.43)g and ( 0.23?0.09)g, respectively;the tumor inhibition rate was 0,43.1%,47.1% and 84.9%, respectively;the hepatic metastasis rate was 85.7%,78.6%,21.4% and 0 respectively.MVD in IFN-? group and combined group was significant lower than that in control group and 5-FU group. Conclusions IFN-? can inhibit the growth and hepatic metastasis of orthotopic implanted human colon cancer by inhibiting the tumor angiogenesis.It may be more effective when INF-? combined with cyotoxic agents.

Objective To investigate the clinical characteristics, diagnosis and treatment of placenta increta.Methods A retrospective analysis was carried out on 13 admitted cases of placenta increta from 1989～2006. Results Among the 13 cases analysed, 5 cases with a history of Caesarian section had a 0% success rate of treatment with conservative care (0/5), 100% less than that of cases with no history of Caesarian section (8/8), P＜0.05; the success rate of treatment of partial placenta increta with methotrexate with Jia Wei Sheng Hua Tang was 100%. Conclusion Caesarian section is a risk factor of placenta increta, and its prognosis is poor; however, Jia Wei Sheng Hua Tang has proven satisfactory as a supplementary treatment for placenta inereta.

Objective To investigate the prenatal or intrapartum risk factors,resuscitation and its outcomes among full term but dying newborns after severe asphyxia (Apgar score was 0～1 at 1 min after birth).Methods Totally,39 dying full-term newborns,who were admitted to the neonatal intensive care unit of Shenzhen Maternity&Child Healthcare Hospital,Southern Medical University from January,2003 to June,2014 were analyzed retrospectively.Information on perinatal influencing factors and outcomes of resuscitation were collected and analyzed with Chi-square test.Results All of the babies were divided into died (n=7,18％,one refused to further treatment) and survived group [n=32,among which 15 suffered from hypoxic-ischemic encephalopathy(HIE) (seven mild,seven moderate and one severe case)].Fetal heart beat was undetectable in more babies in the died group than in the survived group [2/7 vs 0％(0/32),x2=4.695,P=0.028].There were 19 infants with Apgar score ≤ 5 at 5 min,and seven of them died (7/19).The other 12 survived with HIE occurred in eight cases (four mild,three moderate and one severe case) and the rest four babies did not report any neurological complications.Among the ten infants with Apgar score ≤ 5 at 10 min,seven died or refused to treatment and the other three survived with moderate HIE.Conclusions Most of the dying term babies may have promising outcomes after effective resuscitation,especially for those with Apgar score over 5 at 5 min.However,for those dying term babies with Apgar score remains ≤ 5 at 5 min,management after resuscitation should be strengthened to reduce the occurrence of any sequelae or complications.

Background and purpose:Early evaluating the therapeutic efficacy of transcatheter arterial chemoembolization (TACE) in patients with hepatic cancer is still a diffcult clinical problem. The purpose of this study was to evaluate the ability of the apparent diffusion coeffcient (ADC) to help predict early disease progression after TACE.Methods:Institutional review board approval was obtained, and all patients signed informed consent. Magnetic resonance imaging (MRI) and diffusion-weighted imaging (DWI) (b=50, 500, 1 000 mm2/s) were performed before and 1 month after initiating TACE for 23 patients with hepatic cancer (14 were male, 9 were female; mean age: 53.3 years;range: 21-85 years). Contrast-enhanced MRI was performed 3 months after initiating TACE. Patients were classiifed as either progressing or non-progressing according to RECIST 1.1. The preoperative ADC values of tumor and the ADC values of tumor 1 month after TACE were analyzed by pairedt-test in both progressing and non-progressing group. Unpairedt-test was used to compare ADC parameters between progressing and non-progressing group. In all the 23 hepatic cancer patients, receiver operating characteristic (ROC) curve analysis was performed to determine a threshold ADC ratio (ADC%) to differentiate progressing from non-progressing patients.Results:Thirteen progressing and 9 non-progressing patients were evaluated. Increase in ADCs of tumor was observed in non-progressing patients at 1 month after TACE compared with preoperative ADCs. There was a signiifcant difference between the 2 groups (P=0.01). In progressing group, preoperative ADCs of tumor were similar to those at 1 month after TACE (P=0.221). There was no significant difference in preoperative ADCs of tumor and ADC% between the progressing and non-progressing groups. In patients with hepatic cancer, 1 month ADC ratio in non-progressing patients were signiifcantly higher than those of progressing patients (P=0.029). Using ROC to evaluate the ability of ADC% could predict early disease pro-gression after TACE. Using -6.455% as the threshold, the area under the ROC curve was 0.867 (95%CI: 0.643-1.000). The sensitivity was 100%, and the speciifcity was 66.7%.Conclusion:One month after TACE, the increases in ADCs of tumor were observed only in the non-progressing group; and the ADC ratio seems to be a promising tool for helping predict the early disease progression after TACE in patients with hepatic cancer.