Objective To investigate T cell-mediated effects of dendritic cells(DC)transfected with kidney associated antigen G250 gene using adenovirus(Ad)as vector in the treatment of kidney cancer. Methods Peripheral blood mononuclear cells(PBMC)were isolated by standard FicollPaque density gradient centrifugation of heparinized blood obtained from healthy donors.After removal of the nonadherent ceils,the adherent cells were divided into gene transfer group,protein pulsed group and control group.The gene transfer group was infected with Ad/G250 virus;the protein pulsed group was added with G250 protein; the control group was added with PBS.The DCs were cultured and proliferated in vitro using recombinant human granulocyte macrophage colony stimulating factor and inlerleukin-4.The autologous T cells were added into the 3 DC groups(responders:dendritics,20:1).After 5 d co-culture,3 eytotoxic T lymphocyte(CTL)groups were collected.TheG250 mRNA expression was detected in transduced DCs using RT-PCR.The G250 protein translation products and cell surface marker of DCs were analyzed by flow cytometry.The specific cytolytic activities of CTL to different target cells(renal carcinoma 786-0 cell line and lung cancer A549 cell line)were assessed by MTT method. Results Ad/G250 transfected DC successfully and the G250 expression was confirmed by RT-PCR. The expression levels of CD_(80), CD_(83). HLA-DR and CD_(86) in the Ad/G250 group were higher than those in the other 2 groups. The cytotoxicity to 786-0 of T lymphocytes activated by Ad/G250 transfecting DCs(83. 4±2. 8)% was greater than those of T lymphocytes in the protein pulsed group(79. 6±2. 4)% and control group(77. 3±2. 1)%. There were no significant differences of the cytotyxicities to A549 among the 3 cytotoxic T lymphocyte groups(F=0. 373.P=0.693). Conclusions Ad-loading DCs may be useful for immunotherapeutic protocols against self-antigens for the kidney cancer.

Objective To observe the changes of serum IGF-I,VEGF levels in premature infants,and to dis-cuss the predicting values for the development of ROP;Methods Preterm infants admitted into those whose birth weight ( BW)≤1 500g and ( or) gestational age ( GA)≤31 weeks joined in ROP-screen,and those who were diag-nosed as ROP according to ICROP were included into ROP group.In the same time,those who didn't develop ROP were divided into the control group.Serum IGF-I,VEGF were measured one week from their birth or correct gestational age of 31 weeks.Serum IGF-I, VEGF were evaluated with ELISA method.Results The concentration of IGF-I, VEGF:Group ROP[(20.65 ±11.11)μg/L,(195.61 ±105.98)ng/L] were all significantly lower than the control group [(42.86 ±18.72)μg/L,(363.59 ±101.08)ng/L](all P<0.01).There were significant different of birth weight[ROP group (1 229.69 ±232.37)g,the control group (1 401.32 ±171.78)g;t=4.144)],GM[ROP group (3.20 ±1.23)mmol/L,the control group (3.70 ±1.17)mmol/L;t=2.082],the time of oxygen inhaling[ROP group (32 ±22)d,the control group (21 ±12)d;t=-2.856],oxygen inhalation mode(the percentage of mechanical ventilation)[ROP group 33/48(68.8%),the control group 16/50(32.0%);χ2 =16.363] and fertilization way(the percentage of test tube baby)[ROP group 13/48(27.1%),the control group 4/50(8.0%);χ2 =6.262] between ROP and control group (all P<0.05).Conclusion The low serum IGF-I(<28.08μg/L),VEGF(<232.89ng/L) of a premature in the first week or correct gestational age of 31 weeks and (or)BW≤1 500g would probably suggest the development of ROP.

This paper collected the traditional Chinese medicine (TCM) prescriptions of osteoporosis and osteoarthritis from CNKI to set up database and used TCM inheritance system to analyze the composing principles in prescriptions. We collected 103 and 106 prescriptions for osteoporosis and osteoarthritis respectively, then analyzed inherent principles between herbs in prescriptions by using the TCM inheritance system, and obtained the frequency of each herb, and drug combinations of two diseases' prescriptions. The result showed that the principles in TCM prescriptions of osteoporosis and osteoarthritis are similarities, buch as tonifying kidney and invigorating the circulation of blood, but differences of the specific drugs. That is to say, tonifying kidney is the main method of osteoporosis treatment, however, invigorating the circulation of blood is the main method of osteoarthritis treatment.
Blood Circulation ; drug effects ; Databases, Factual ; Drug Prescriptions ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Humans ; Kidney ; drug effects ; physiopathology ; Osteoarthritis ; drug therapy ; physiopathology ; Osteoporosis ; drug therapy ; physiopathology

BACKGROUND:Liver cancer pathogenesis and intervention have attracted increasing attentions. Mesenchymal stem cel s become a popular tool for cel cancer research, because of their low immunogenicity and tumor tropism. At present, mesenchymal stem cel s have been applied to the study of liver cancer.
OBJECTIVE:To summarize the advances of mesenchymal stem cel s used in liver cancer in basic and clinical research.
METHODS:An online retrieval of CNKI and Pubmed database was performed by the first author for the articles about mesenchymal stem cel s and effect of modified mesenchymal stem cel s on hepatoma carcinoma cel s published from January 2004 to January 2013. The key words were“mesenchymal stem cel , liver cancer, tumor”in Chinese and English. Repetitive research was excluded, and 47 studies met the inclusion criteria.
RESULTS AND CONCLUSION:Stem cel s are seldom reported in liver cancer, and the limited present study show that mesenchymal stem cel s may have a certain influence on the hepatoma carcinoma cel proliferation, invasion and biological behavior. However, due to the differences of cel lines used by the various laboratories, experimental conditions, animal models, as wel as infusion means of stem cel s, experimental results are also inconsistent. Scholars have conducted a series of studies on the mechanism of the Wnt pathway and tumor necrosis factor-related apoptosis-inducing ligand pathway. Tropism of mesenchymal stem cel s to tumor cel s, including liver cancer is widely recognized, so scholars imported therapeutic genes and drugs into mesenchymal stem cel s to interfere with the development of liver cancer, and have achieved some progress. This evidence provides new avenues for cel therapy for liver cancer. Less safety studies in vivo and clinical trials of mesenchymal stem cel s are available, therefore security risks deserve further research.

Objective To compare the clinical efficacy of moxibustion Zhiyin with knee chest decubitus,ear acupoint magnetic bead press combined with knee chest decubitus and knee chest decubitus in correction of breech position,thus to provide more safe and effective correction method for the clinical treatment.Methods 150 pregnant women with breech were selected.They were randomly divided into observation group Ⅰ,observation group Ⅱ and control group according to the digital table,50 cases in each group.The control group was treated with knee chest decubitus to correct breech position,the observation group Ⅰ was given moxibustion Zhiyin combined with knee chest position,the observation group Ⅱ was given ear acupoint magnetic bead press combined with knee chest decubitus.Results The effective rates of the observation group Ⅰ and observation group Ⅱ were 96％,92％,respectively,which were higher than 76％ of the control group,the difference were statistically significant (χ2=5.09,4.18,all P＜0.05).The effective rate between the observation group Ⅰ and observation group Ⅱ showed no significant difference (P＞0.05).Conclusion Moxibustion Zhiyin combined with knee chest decubitus and ear acupoint magnetic bead press combined with knee chest decubitus in the correction of breech position have short course of treatment,high success rate,and are more economical and convenient,with high safety and good curative effect,which are worthy of promotion.

OBJECTIVE To investigate the incidence of integrons in Pseudomonas aeruginosa strains isolated from blood samples in Peking University People′s Hospital and to analyze the correlation between integrons and drug resistance of P.aeruginosa.METHODS Forty-two strains of clinically isolated P.aeruginosa were collected.The antibiotics susceptibility was tested by K-B methods.Integrase gene of integron was amplified by PCR using degenerate primers.The integrons were classified by restriction fragment length polymorphism(RFLP) analysis of positive PCR products with Hinf Ⅰ restriction enzyme.RESULTS The drug-resistance rates of 42 strains of P.aeruginosa against 20 kinds of antibiotics ranged from 9.5% to 100%.Twenty-three strains were resistant to 12 kinds of antibiotics.Nineteen of the 42 isolates(45.2%) contained integrons,all of which were revealed as class Ⅰ of integrons by RFLP analysis.Neither class Ⅱ nor class Ⅲ of integron was detected.The positive percentage of integrons was increased by years.CONCLUSIONS Class Ⅰ integrons are widespread in isolates from blood samples in our hospital.The presence of integrons is closely associated with multi-drug resistance of P.aeruginosa.

Objective To investigate the expression of phospho-histone H3 (PHH3) protein in pancreatic neuroendocrine neoplasms (PNENs) and explore its potential value for pathological grading of PNENs.Methods Clinical and pathological data of 283 patients with PNENs treated in Changhai Hospital from December 2000 to May 2016 were retrospectively analyzed.PNENs tissue chip was prepared,and immunohistochemistry was used to examine the expression of PHH3 and Ki67.The receiver operating characteristic curve for PHH3 was drawn and the area under the curve(AUC) was calculated to determine the cutoff value for PNENs grading.Ki67 was used for PNENs grading according to domestic and intemational criteria for PNENs classification.The relationship of PHH3 and Ki67 classification with clinical pathological features and prognosis of PNENs as well as the correlation between the two classification methods were analyzed.Results Of 283 patients,132 were male and 151 were female,aged from 16 to 78 years old.The average age was (50 ± 1) years old.There were 44 cases with functional PNENs and 239 with non-functional PNENs.The diameter of tumors ranged from 1.1 cm to 17 cm and the average diameter was (4.1 ± 1.2)cm.According to the Ki67 standard,there were 127,116,33 and 7 cases of Grade G1,G2,pancreatic neuroendocrine tumor(PNET) G3 and pancreatic neuroendocrine cancer(PNEC) G3.According to the PHH3 standard,there were 118,119,3 8 and 8 cases of Grade G1,G2,PNET G3 and PNEC G3.There was a positive correlation between the two grading criteria (r =0.941,P ＜0.001).PHH3 expression and PNENs grading were not correlated with age,gender,tumor functional or not and tumor locations (all P ＞ 0.05),but were both positively correlated with tumor size,histological grade,lymph node metastasis,TNM stage and prognosis (all P ＜0.05).The average time of observing PHH3 and Ki67 staining per case in the immunohistochemistry of tissue chip was 9 min and 45 min,respectively.Conclusions Detection of PHH3 expression in PNENs can be used for the pathological diagnosis,grading and prognostic evaluation,which was more accurate and convenient than Ki67 criteria.

Objective:To inve tigate the value o electron beam tomography and echocardiography or diagno ing the comple congenital heart di ea e . Method :Thirty our patient with comple congenital heart di ea e underwent both echocardiography and electron beam tomography,which wa con irmed by angiocardiography in 18 patient .Twenty one patient were operated on.All data were comparatively analyzed Re ult :The rate o diagno i coincidence in diagno ing comple congenital heart di ea e wa 82 4% or electron beam tomography,and 73 5% or echocardiography.The accuracy o electron beam tomography in diagno ing e tracardiac abnormal tructure wa 97 9% and obviou ly higher than 53 2% by echocardiography.The accuracy o echocardiography in diagno ing intracardiac abnormal tructure wa 95 9% and higher than 81 6% by electron beam tomography.Electron beam tomography and echocardiography had a imilar accuracy or diagno ing abnormal tructure at the junction o ve el with cardiac chamber. Conclu ion:Electron beam tomography combined with echocardiography can greatly improve the diagno i accuracy o comple congenital heart di ea e and can al o reduce the u e o inva ive angiocardiography.

Adolescent ; Humans ; Male ; Pigmentation Disorders ; pathology ; Skin ; pathology

Objective To investigate etiology , clinical characteristics and imaging expression of senile brain stem infarct. Methods The etiology and characteristics of clinic, CT and MRI of 51 cases had been summarized . Results The main causes had hypertension, diabetes mellitus and hyperlipidemia, symptoms complicated, frequently occuring site was brain pontine. Conclusion The main clinical appearances of brain stem infarct are complicated and varies, including typical cranial and cross symptoms, and non-typical early diagnosis, so it is easy to misdiagnose and miss diagnose, MRI had unique advantage in the diagnosis of brain stem infarct .